PTCH2 (NM_003738) Human Untagged Clone

CAT#: SC303371

PTCH2 (untagged)-Human patched 2 (PTCH2), transcript variant 1



  "NM_003738" in other vectors (4)

CNY 23,280.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


PTCH2 mouse monoclonal antibody, clone OTI1F5 (formerly 1F5)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms PTC2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC303371 representing NM_003738.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGACTCGATCGCCGCCCCTCAGAGAGCTGCCCCCGAGTTACACACCCCCAGCTCGAACCGCAGCACCC
CAGATCCTAGCTGGGAGCCTGAAGGCTCCACTCTGGCTTCGTGCTTACTTCCAGGGCCTGCTCTTCTCT
CTGGGATGCGGGATCCAGAGACATTGTGGCAAAGTGCTCTTTCTGGGACTGTTGGCCTTTGGGGCCCTG
GCATTAGGTCTCCGCATGGCCATTATTGAGACAAACTTGGAACAGCTCTGGGTAGAAGTGGGCAGCCGG
GTGAGCCAGGAGCTGCATTACACCAAGGAGAAGCTGGGGGAGGAGGCTGCATACACCTCTCAGATGCTG
ATACAGACCGCACGCCAGGAGGGAGAGAACATCCTCACACCCGAAGCACTTGGCCTCCACCTCCAGGCA
GCCCTCACTGCCAGTAAAGTCCAAGTATCACTCTATGGGAAGTCCTGGGATTTGAACAAAATCTGCTAC
AAGTCAGGAGTTCCCCTTATTGAAAATGGAATGATTGAGCGGATGATTGAGAAGCTGTTTCCGTGCGTG
ATCCTCACCCCCCTCGACTGCTTCTGGGAGGGAGCCAAACTCCAAGGGGGCTCCGCCTACCTGCCCGGC
CGCCCGGATATCCAGTGGACCAACCTGGATCCAGAGCAGCTGCTGGAGGAGCTGGGTCCCTTTGCCTCC
CTTGAGGGCTTCCGGGAGCTGCTAGACAAGGCACAGGTGGGCCAGGCCTACGTGGGGCGGCCCTGTCTG
CACCCTGATGACCTCCACTGCCCACCTAGTGCCCCCAACCATCACAGCAGGCAGGCTCCCAATGTGGCT
CACGAGCTGAGTGGGGGCTGCCATGGCTTCTCCCACAAATTCATGCACTGGCAGGAGGAATTGCTGCTG
GGAGGCATGGCCAGAGACCCCCAAGGAGAGCTGCTGAGGGCAGAGGCCCTGCAGAGCACCTTCTTGCTG
ATGAGTCCCCGCCAGCTGTACGAGCATTTCCGGGGTGACTATCAGACACATGACATTGGCTGGAGTGAG
GAGCAGGCCAGCACAGTGCTACAAGCCTGGCAGCGGCGCTTTGTGCAGCTGGCCCAGGAGGCCCTGCCT
GAGAACGCTTCCCAGCAGATCCATGCCTTCTCCTCCACCACCCTGGATGACATCCTGCATGCGTTCTCT
GAAGTCAGTGCTGCCCGTGTGGTGGGAGGCTATCTGCTCATGCTGGCCTATGCCTGTGTGACCATGCTG
CGGTGGGACTGCGCCCAGTCCCAGGGTTCCGTGGGCCTTGCCGGGGTACTGCTGGTGGCCCTGGCGGTG
GCCTCAGGCCTTGGGCTCTGTGCCCTGCTCGGCATCACCTTCAATGCTGCCACTACCCAGGTGCTGCCC
TTCTTGGCTCTGGGAATCGGCGTGGATGACGTATTCCTGCTGGCGCATGCCTTCACAGAGGCTCTGCCT
GGCACCCCTCTCCAGGAGCGCATGGGCGAGTGTCTGCAGCGCACGGGCACCAGTGTCGTACTCACATCC
ATCAACAACATGGCCGCCTTCCTCATGGCTGCCCTCGTTCCCATCCCTGCGCTGCGAGCCTTCTCCCTA
CAGGCGGCCATAGTGGTTGGCTGCACCTTTGTAGCCGTGATGCTTGTCTTCCCAGCCATCCTCAGCCTG
GACCTACGGCGGCGCCACTGCCAGCGCCTTGATGTGCTCTGCTGCTTCTCCAGTCCCTGCTCTGCTCAG
GTGATTCAGATCCTGCCCCAGGAGCTGGGGGACGGGACAGTACCAGTGGGCATTGCCCACCTCACTGCC
ACAGTTCAAGCCTTTACCCACTGTGAAGCCAGCAGCCAGCATGTGGTCACCATCCTGCCTCCCCAAGCC
CACCTGGTGCCCCCACCTTCTGACCCACTGGGCTCTGAGCTCTTCAGCCCTGGAGGGTCCACACGGGAC
CTTCTAGGCCAGGAGGAGGAGACAAGGCAGAAGGCAGCCTGCAAGTCCCTGCCCTGTGCCCGCTGGAAT
CTTGCCCATTTCGCCCGCTATCAGTTTGCCCCGTTGCTGCTCCAGTCACATGCTAAGGCCATCGTGCTG
GTGCTCTTTGGTGCTCTTCTGGGCCTGAGCCTCTACGGAGCCACCTTGGTGCAAGACGGCCTGGCCCTG
ACGGATGTGGTGCCTCGGGGCACCAAGGAGCATGCCTTCCTGAGCGCCCAGCTCAGGTACTTCTCCCTG
TACGAGGTGGCCCTGGTGACCCAGGGTGGCTTTGACTACGCCCACTCCCAACGCGCCCTCTTTGATCTG
CACCAGCGCTTCAGTTCCCTCAAGGCGGTGCTGCCCCCACCGGCCACCCAGGCACCCCGCACCTGGCTG
CACTATTACCGCAACTGGCTACAGGGAATCCAGGCTGCCTTTGACCAGGACTGGGCTTCTGGGCGCATC
ACCCGCCACTCGTACCGCAATGGCTCTGAGGATGGGGCCCTGGCCTACAAGCTGCTCATCCAGACTGGA
GACGCCCAGGAGCCTCTGGATTTCAGCCAGCTGACCACAAGGAAGCTGGTGGACAGAGAGGGACTGATT
CCACCCGAGCTCTTCTACATGGGGCTGACCGTGTGGGTGAGCAGTGACCCCCTGGGTCTGGCAGCCTCA
CAGGCCAACTTCTACCCCCCACCTCCTGAATGGCTGCACGACAAATACGACACCACGGGGGAGAACCTT
CGCATCCCGCCAGCTCAGCCCTTGGAGTTTGCCCAGTTCCCCTTCCTGCTGCGTGGCCTCCAGAAGACT
GCAGACTTTGTGGAGGCCATCGAGGGGGCCCGGGCAGCATGCGCAGAGGCCGGCCAGGCTGGGGTGCAC
GCCTACCCCAGCGGCTCCCCCTTCCTCTTCTGGGAACAGTATCTGGGCCTGCGGCGCTGCTTCCTGCTG
GCCGTCTGCATCCTGCTGGTGTGCACTTTCCTCGTCTGTGCTCTGCTGCTCCTCAACCCCTGGACGGCT
GGCCTCATAGTGCTGGTCCTGGCGATGATGACAGTGGAACTCTTTGGTATCATGGGTTTCCTGGGCATC
AAGCTGAGTGCCATCCCCGTGGTGATCCTTGTGGCCTCTGTAGGCATTGGCGTTGAGTTCACAGTCCAC
GTGGCTCTGGGCTTCCTGACCACCCAGGGCAGCCGGAACCTGCGGGCCGCCCATGCCCTTGAGCACACA
TTTGCCCCCGTGACCGATGGGGCCATCTCCACATTGCTGGGTCTGCTCATGCTTGCTGGTTCCCACTTT
GACTTCATTGTAAGGTACTTCTTTGCGGCGCTGACAGTGCTCACGCTCCTGGGCCTCCTCCATGGACTC
GTGCTGCTGCCTGTGCTGCTGTCCATCCTGGGCCCGCCGCCAGAGGTGATACAGATGTACAAGGAAAGC
CCAGAGATCCTGAGTCCACCAGCTCCACAGGGAGGCGGGCTTAGGTGGGGGGCATCCTCCTCCCTGCCC
CAGAGCTTTGCCAGAGTGACTACCTCCATGACCGTGGCCATCCACCCACCCCCCCTGCCTGGTGCCTAC
ATCCATCCAGCCCCTGATGAGCCCCCTTGGTCCCCTGCTGCCACTAGCTCTGGCAACCTCAGTTCCAGG
GGACCAGGTCCAGCCACTGGGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_003738
Insert Size 3612 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003738.4
RefSeq Size 3773 bp
RefSeq ORF 3612 bp
Locus ID 8643
UniProt ID Q9Y6C5
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
MW 130.5 kDa
Gene Summary This gene encodes a transmembrane receptor of the patched gene family. The encoded protein may function as a tumor suppressor in the hedgehog signaling pathway. Alterations in this gene have been associated with nevoid basal cell carcinoma syndrome, basal cell carcinoma, medulloblastoma, and susceptibility to congenital macrostomia. Alternatively spliced transcript variants have been described.[provided by RefSeq, Oct 2009]
Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...