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Troponin T1 (TNNT1) (NM_003283) Human Untagged Clone
CAT＃: SC303290
TNNT1 (untagged)-Human troponin T type 1 (skeletal, slow) (TNNT1), transcript variant 1
CNY 2,400.00
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ANM; NEM5; STNT; TNT; TNTS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC303290 sequence for NM_003283 edited (data generated by NextGen Sequencing)
ATGTCGGACACCGAGGAGCAGGAATATGAGGAGGAGCAGCCGGAAGAGGAGGCTGCGGAG GAGGAGGAGGAAGCCCCCGAAGAGCCGGAGCCGGTGGCAGAGCCAGAAGAGGAACGCCCC AAACCAAGCCGCCCCGTGGTGCCTCCTTTGATCCCGCCAAAGATCCCAGAAGGGGAGCGC GTTGACTTCGATGACATCCACCGCAAGCGCATGGAGAAAGACCTGCTGGAGCTGCAGACA CTCATCGATGTACATTTCGAGCAGCGGAAGAAGGAGGAAGAGGAGCTGGTTGCCTTGAAG GAGCGCATTGAGCGGCGCCGGTCAGAGAGAGCCGAGCAACAGCGCTTCAGAACTGAGAAG GAACGCGAACGTCAGGCTAAGCTGGCGGAGGAGAAGATGAGGAAGGAAGAGGAAGAGGCC AAGAAGCGGGCAGAGGATGATGCCAAGAAAAAGAAGGTGCTGTCCAACATGGGGGCCCAT TTTGGCGGCTACCTGGTCAAGGCAGAACAGAAGCGTGGTAAGCGGCAGACGGGGCGGGAG ATGAAGGTGCGCATCCTCTCCGAGCGTAAGAAGCCTCTGGACATTGACTACATGGGGGAG GAACAGCTCCGGGCCCGGTCTGCCTGGCTGCCTCCATCACAGCCCTCCTGCCCTGCCAGG GAGAAAGCCCAGGAGCTGTCGGACTGGATCCACCAGCTGGAGTCTGAGAAGTTCGACCTG ATGGCGAAGCTGAAACAGCAGAAATATGAGATCAACGTGCTGTACAACCGCATCAGCCAC GCCCAGAAGTTCCGGAAGGGGGCAGGGAAGGGCCGCGTTGGAGGCCGCTGGAAGTGA Clone variation with respect to NM_003283.4 >OriGene 5' read for NM_003283 unedited
AGGNCGGCCCGCGNAATTCGGCCATTACGGCCGGGGAGCAAGGCTCAGCCTCAANATTCA CAGCATCTCAGACGCAGCCTAGGCCGCACCAGGATGTCGGACACCGAGGAGCAGGAATAT GAGGAGGAGCAGCCGGAAGAGGAGGCTGCGGAGGAGGAGGAGGAAGCCCCCGAAGAGCCG GAGCCGGTGGCAGAGCCAGAAGAGGAACGCCCCAAACCAAGCCGCCCCGTGGTGCCTCCT TTGATCCCGCCAAAGATTCCCAGAAGGGGAGCGCGTTGACTTCGATGACATCCACCGCAA GCGCATGGAGAAAGACCTGCTGGAGCTGCAGACACTCATCGATGTACATTTCGAGCAGCG GAAGAAGGAGGAAGAGGAGCTGGTTGCCTTGAAGGAGCGCATTGAGCGGCGCCGGTCAGA GAGAGCCGAGCAACAGCGCTTCAGAACTGAGAAGGAACGCGAACGTCAGGCTAAGCTGGC GGAGGAGAAGATGAGGAAGGAAGAGGAAGAGGCCAAGAAGCGGGCAGAGGATGATGCCAA GAAAAAGAAGGTGCTGTCCAACATGGGGGCCCATTTTGGCGGCTACCTGGTCAAGGCAGA ACAGAAGCGTGGTAAGCGGCAGACGGNGCGGGAGATGAAGGTGCGCATCCTCTCCGAGCG TAAGAAGCCTCTGGACATTGACTACTGGNGGGGAGGAACCAGCTCGGNNCCNGTCTGCCT GGGCTGCCTCCATCACAGCCCTCCTGCCCTGCCNAGGGAGAAAGCCCAGGAGCTGTTCGA CTGGNATCACCAGCTGGAGTCTGGAAAGTTCGACCTGATGGGCGAGCTTGAACAGCGAAT TTGAAATCACGTGCTG >OriGene 3' read for NM_003283 unedited
AGNAAAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGCTCTGTTCAGGAAACAGCTA TGACCGCGGCCGCAATCTAGAGTCGAGGGCCGAGGCGGCCGACATGTTTTTTTTTTTTTT TTTTTTTTTTTTTTTAGCGGGTGTCTGAGGGGAGCGTTTATTTCAAGCTACCGATGGGAC AAACACTCCCAGGCTTCCCAGGTGCCACTGTCCGGGGCGGCATCCTCACTTCCAGCGGCC TCCAACGCGGCCCTTCCCTGCCCCCTTCCGGAACTTCTGGGCGTGGCTGATGCGGTTGTA CAGCACGTTGATCTCATATTTCTGCTGTTTCAGCTTCGCCATCAGGTCGAACTTCTCAGA CTCCAGCTGGTGGATCCAGTCCGACAGCTCCTGGGCTTTCTCCCTGGCAGGGCAGGAGGG CTGTGATGGAGGCAGCCAGGCAGACCGGGCCCGGAGCTGTTCCTCCCCCATGTAGTCAAT GTCCAGAGGCTTCTTACGCTCGGAGAGGATGCGCACCTTCATCTCCCGCCCCGTCTGCCG CTTACCACGCTTCTGTTCTGCCTTGACCAGGTAGCCGCCAAAATGGGCCCCCATGTTGGA CAGCACCTTCTTTTTCTTGGCATCATCCTCTGCCCGCTTCTTGGCCTCTTCCTCTTCCTT CCTCATCTTCTCCTCCGCCAGCTTAGCCTGACGTTCGCGTTCCTTCTCAGTTCTGAAGCG CTGTTGCTTCGCTCTCTCTGACCGGCGCCGCTCAATGCGCTCCTTCAAGGGCACCAGCTN CTCTTCCTCCTTTCTCCGCTGCTCGAAAGTACATCGATGAGTGTCTGCAGCTCCAGCAGG TCTTTCTCCATGCGCTTGCGGTGGGTGTCATCCAAATCAACGCG |
| Restriction Sites | Please inquire |
| ACCN | NM_003283 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003283.3, NP_003274.2 |
| RefSeq Size | 980 bp |
| RefSeq ORF | 837 bp |
| Locus ID | 7138 |
| UniProt ID | P13805 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longest isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221318 | TNNT1 (Myc-DDK-tagged)-Human troponin T type 1 (skeletal, slow) (TNNT1), transcript variant 1 |
CNY 2,400.00 |
|
| RC221318L3 | Lenti ORF clone of Human troponin T type 1 (skeletal, slow) (TNNT1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221318L4 | Lenti ORF clone of Human troponin T type 1 (skeletal, slow) (TNNT1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG221318 | TNNT1 (tGFP-tagged) - Human troponin T type 1 (skeletal, slow) (TNNT1), transcript variant 1 |
CNY 4,000.00 |
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