United States
Germany
Japan
United Kingdom
China
RFNG (NM_002917) Human Untagged Clone
CAT＃: SC303249
RFNG (untagged)-Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG)
CNY 2,400.00
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_002917 edited
ATGAGCCGCGCGCGTGGGGCGCTGTGCCGGGCCTGCCTCGCGCTGGCCGCGGCCCTGGCC GCGCTGCTGTTACTGCCGCTGCCGCTGCCCCGCGCGCCCGCCCCGGCCCGGACCCCCGCC CCGGCCCCGCGCGCGCCCCCGTCCCGGCCCGCTGCCCCCAGCCTGCGGCCTGACGACGTC TTCATCGCCGTCAAGACCACCCGGAAGAACCACGGGCCGCGCCTGCGGCTGCTGCTGCGC ACCTGGATCTCCCGGGCCCGCCAGCAGACGTTTATCTTCACCGACGGGGACGACCCTGAG CTCGAGCTCCAGGGCGGCGACCGTGTCATCAACACCAACTGCTCGGCGGTGCGCACTCGT CAGGCCCTCTGCTGCAAGATGTCCGTGGAGTATGACAAGTTCATTGAGTCCGGGCGCAAG TGGTTTTGCCACGTGGATGATGACAATTATGTGAACGCAAGGAGCCTCCTGCACCTGCTC TCCAGCTTCTCACCCAGCCAGGACGTCTACCTGGGGCGGCCCAGCCTGGACCACCCCATT GAGGCCACCGAGAGGGTCCAGGGTGGCAGAACTGTGACCACGGTCAAGTTCTGGTTTGCT ACTGGTGGGGCCGGGTTCTGCCTCAGCAGAGGCCTTGCCCTCAAGATGAGCCCATGGGCC AGCCTGGGCAGCTTCATGAGCACAGCTGAGCAGGTGCGGCTGCCGGATGACTGCACAGTT GGCTACATCGTGGAGGGGCTCCTGGGCGCCCGCCTGCTGCACAGCCCCCTCTTCCACTCT CACCTGGAGAACCTGCAGAGGCTGCCGCCCGACACCCTGCTCCAGCAGGTTACCTTGAGC CATGGGGGTCCTGAGAACCCACAGAACGTGGTGAACGTGGCTGGAGGCTTCAGCCTGCAT CAAGACCCCACACGGTTTAAGTCTATCCATTGTCTTCTGTACCCAGACACGGACTGGTGT CCCAGGCAGAAACAGGGCGCCCCGACCTCTCGGTGA >OriGene 5' read for NM_002917 unedited
GACCTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCACCAGGTCCCGGCGGCCG CATGAGCCGCGCGCGTGGGGCGCTGTGCCGGGCCTGCCTCGCGCTGGCCGCGGCCCTGGC CGCGCTGCTGTTACTGCCGCTGCCGCTGCCCCGCGCGCCCGCCCCGGCCCGGACCCCCGC CCCGGCCCCGCGCGCGCCCCCGTCCCGGCCCGCTGCCCCCAGCCTGCGGCCTGACGACGT CTTCATCGCCGTCAAGACCACCCGGAAGAACCACGGGCCGCGCCTGCGGCTGCTGCTGCG CACCTGGATCTCCCGGGCCCGCCAGCAGACGTTTATCTTCACCGACGGGGACGACCCTGA GCTCGAGCTCCAGGGCGGCGACCGTGTCATCAACACCAACTGCTCGGCGGTGCGCACTCG TCAGGCCCTCTGCTGCAAGATGTCCGTGGAGTATGACAAGTTCATTGAGTCCGGGCGCAA GTGGTTTTGCCACGTGGATGATGACAATTATGTGAACGCAAGGAGCCTCCTGCACCTGCT CTCCAGCTTCTCACCCAGCCAGGACGTCTACCTGGGGCGGCCCACCCTGGACCACCCCAT TGAGGCCACCGAGAGGGTCCAGGGTGGCAGAACTGTGACCACGGTCAAGTTCTGGTTTGC TACTGGTGGGGCCGGCTTCTGCCTCAGCAGAGGCCTTGCCCTCAAGATGAGCCAATGGGC TAGCCTGAGCAGATTCATGAGCACAGCTGAACAAGTGCGGCTGCCGGATGACCTGCCCAG CTGGCTACTCGAGGACGGGCCTCCATGGGACGCCCAGCTGCTGCACAGCACCCTCTTCCA GTCTCCACTGGCAGAATCGTGGCAAAGCGTGCTCGTCAGACAGCAGGATCGGATAGCTTA CCTGAAGCCAGGTGGGGGTGCTGGAGATACCACAGACAGCGATGAATAGAGATTAGGAGG CATAGA >Forward primer walk for NM_002917 unedited
CGCTTTAGTCTATCCTTGTCTTCTGTACCCAGGCACGGACTGGTGTCCCATGCAGAAACA GGGCGCCCCGACCTCTCGGTGACACCAACCACCCCGACCCAGGGCTGCCTGGCTCTGTCC CAGGCGCGGGGAACCAGAGCCCCCTATGGGCTCAGTGGGCTCCCTCAGGTGCCACGGCCG CACCAGTGAGATGCAGGCACCTGGCAGACCCTCTGGCTAGCCTGCAGCCCCCCCTCTCCC AGCCCCTGGTGGGCTGTGGTGATGGGTGTTTTGGGAGAACGAAGACAGCCAGGCTGATGG CCAGGGCCGCAGTGCCCCTCCCCCCGACCCAGCCCCAAGGTTGATCCCACGGGAACAGGC TTCCACCCCAGCACTTGCGCACCTGGGAGGGAGCTGCCATCCGGGCTCCATTACCTGTTG CTGAAGGCGGGTGCTAGGCTGGCTGGGTGTCAAGGAGCAGGCTCCAGGCCAAGGTCCTGG CCCAGCCACGGCCATTGCAAGGGCTCAGCCTGGCAGGCTTTGTGGGGGACGCCGCCCTCT CTGCCGCAGGCTGGGTGCACGGCCAGGCACCACAGTGGGACTCAGCCCGGGAAGGTCATG TTCTCGACCAGAGCTTTGCTCCCAGTCCAGGCGCCTCATTCGGAGGCCTCTTGACTGGGA CCACAGAGATGTTTTCTCCGCTCTGACTTGTGGCTCAGGACTACTTTCTGGGTCGTGCTC CTGCCCCCACTGTGCCTGGGCCCCATAAACAAACAGGAGCCTTTTGTTCCGCTGACCTGC CCATCCCAGCAGAACCCACCTCCCCGCCTGGTGCCTTTCCATGGAAGGGAAATGGGAACA GGGCCGTCATCTCCCCTCTGCCTCCTGCTTTGCTGTTGCCCGAGCACGAGTAAAGCTTTT GTTTCTTAACTTCACAGAAAACGACAGATA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002917 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002917.1, NP_002908.1 |
| RefSeq Size | 1828 bp |
| RefSeq ORF | 996 bp |
| Locus ID | 5986 |
| UniProt ID | Q9Y644 |
| Protein Families | Transmembrane |
| Protein Pathways | Notch signaling pathway |
| Gene Summary | Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in enhancement of NOTCH1 activation by DLL1 and JAG1. May be involved in limb formation and in neurogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224631 | RFNG (Myc-DDK-tagged)-Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG) |
CNY 2,400.00 |
|
| RC224631L3 | Lenti-ORF clone of RFNG (Myc-DDK-tagged)-Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG) |
CNY 5,890.00 |
|
| RC224631L4 | Lenti-ORF clone of RFNG (mGFP-tagged)-Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG) |
CNY 5,890.00 |
|
| RG224631 | RFNG (tGFP-tagged) - Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG) |
CNY 4,370.00 |
