Dystonin (DST) (NM_001723) Human Untagged Clone
CAT#: SC303058
DST (untagged)-Human dystonin (DST), transcript variant 1e
CNY 51,020.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | BP240; BPA; BPAG1; CATX-15; CATX15; D6S1101; DMH; DT; EBSB2; HSAN6; MACF2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC303058 representing NM_001723.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCACAGTAGTAGTTATAGTTACCGTAGCAGTGATTCTGTGTTTAGTAACACTACCAGCACTCGAACC AGTCTTGATTCAAATGAAAATCTTCTCTTGGTTCATTGTGGTCCAACACTGATCAACTCTTGCATTAGC TTCGGCAGTGAATCCTTTGATGGACACAGGTTAGAAATGTTGCAACAGATTGCCAACAGAGTTCAGAGG GACAGTGTCATCTGTGAAGACAAACTGATTCTTGCTGGAAATGCTCTTCAGTCTGATTCTAAAAGATTA GAATCAGGAGTGCAGTTTCAGAATGAAGCAGAAATTGCTGGGTATATACTTGAATGTGAGAACCTTTTA CGCCAGCATGTAATTGATGTACAGATTCTTATTGATGGAAAATACTACCAGGCAGATCAATTGGTACAG AGGGTTGCAAAACTGCGTGACGAAATTATGGCCTTAAGGAACGAATGTTCTTCTGTGTACAGCAAAGGA CGCATACTGACAACAGAACAGACAAAGCTCATGATATCAGGAATCACTCAAAGTTTAAACTCAGGATTT GCACAGACCTTACACCCTAGTCTGACCTCAGGGCTGACCCAGAGTTTAACACCTTCCCTAACCTCTTCT AGTATGACTTCTGGCCTGTCATCAGGGATGACTTCCCGCCTGACTCCATCTGTCACTCCAGCTTATACA CCTGGTTTCCCATCAGGATTAGTTCCAAATTTCAGTTCAGGAGTAGAGCCAAATTCATTGCAAACTTTG AAGTTGATGCAGATCCGAAAACCCCTTCTAAAGTCTTCTTTGCTGGATCAAAATTTAACAGAAGAAGAA ATCAATATGAAATTTGTTCAGGATCTTTTGAATTGGGTTGATGAGATGCAGGTACAACTGGACCGCACT GAGTGGGGCTCAGATTTGCCAAGTGTTGAAAGCCATTTAGAAAATCATAAAAATGTTCATAGAGCTATT GAAGAATTTGAATCTAGTCTCAAAGAAGCTAAAATCAGTGAGATTCAAATGACAGCACCTCTTAAACTG ACTTATGCAGAAAAGTTGCACAGATTAGAGAGTCAGTATGCAAAACTCTTGAATACATCCAGGAATCAA GAACGGCACCTTGATACACTCCATAATTTTGTAAGTCGTGCGACTAATGAACTTATTTGGTTGAATGAA AAAGAAGAGGAGGAAGTTGCTTATGACTGGAGTGAGAGAAACACCAACATAGCTAGGAAAAAAGATTAT CATGCTGAATTAATGAGAGAACTTGATCAAAAGGAAGAAAATATTAAATCAGTTCAGGAGATAGCAGAG CAGCTACTTCTAGAAAATCATCCAGCCCGGTTAACTATTGAGGCCTACAGAGCGGCAATGCAGACGCAG TGGAGCTGGATCTTACAGCTCTGCCAGTGTGTGGAGCAGCACATAAAGGAGAACACAGCGTATTTCGAG TTTTTCAATGATGCCAAAGAAGCTACTGATTACTTAAGGAATCTAAAAGATGCCATTCAGCGGAAGTAC AGCTGTGATAGATCAAGCAGCATTCACAAGCTAGAAGACCTTGTTCAGGAATCAATGGAAGAGAAAGAA GAACTTCTGCAGTACAAAAGCACTATAGCAAACCTAATGGGAAAAGCAAAAACAATAATTCAACTGAAG CCAAGGAATTCTGACTGTCCACTCAAAACTTCTATTCCGATCAAAGCTATCTGTGACTACAGACAAATT GAGATAACCATTTACAAAGACGATGAATGTGTTTTGGCGAATAACTCTCATCGTGCTAAATGGAAGGTC ATTAGTCCTACTGGGAATGAGGCTATGGTCCCATCTGTGTGCTTCACCGTTCCTCCACCAAACAAAGAA GCGGTGGACCTTGCCAACAGAATTGAGCAACAGTATCAGAATGTCCTGACTCTTTGGCATGAGTCTCAC ATAAACATGAAGAGTGTAGTATCCTGGCATTATCTCATCAATGAAATTGATAGAATTCGAGCTAGCAAT GTGGCTTCAATAAAGACAATGCTACCTGGTGAACATCAGCAAGTTCTAAGTAATCTACAATCTCGTTTT GAAGATTTTCTGGAAGATAGCCAGGAATCCCAAGTCTTTTCAGGCTCAGATATAACACAACTGGAAAAG GAGGTTAATGTATGTAAGCAGTATTATCAAGAACTTCTTAAATCTGCAGAAAGAGAGGAGCAAGAGGAA TCAGTTTATAATCTCTACATCTCTGAAGTTCGAAACATTAGACTTCGGTTAGAGAACTGTGAAGATCGG CTGATTAGACAGATTCGAACTCCCCTGGAAAGAGATGATTTGCATGAAAGTGTGTTCAGAATCACAGAA CAGGAGAAACTAAAGAAAGAGCTGGAACGACTTAAAGATGATTTGGGAACAATCACAAATAAGTGTGAG GAGTTTTTCAGTCAAGCAGCAGCCTCTTCATCAGTCCCTACCCTACGATCAGAGCTTAATGTGGTCCTT CAGAACATGAACCAAGTCTATTCTATGTCTTCCACTTACATAGATAAGTTGAAAACTGTTAACTTGGTG TTAAAAAACACTCAAGCTGCAGAAGCCCTCGTAAAACTCTATGAAACTAAACTGTGTGAAGAAGAAGCA GTTATAGCTGACAAGAATAATATTGAGAATCTAATAAGTACTTTAAAGCAATGGAGATCTGAAGTAGAT GAAAAGAGACAGGTATTCCATGCCTTAGAGGATGAGTTGCAGAAAGCTAAAGCCATCAGTGATGAAATG TTTAAAACGTATAAAGAACGGGACCTTGATTTTGACTGGCACAAAGAAAAAGCAGATCAATTAGTTGAA AGGTGGCAAAATGTTCATGTGCAGATTGACAACAGGTTACGGGACTTAGAGGGCATTGGCAAATCACTG AAGTACTACAGAGACACTTACCATCCTTTAGATGATTGGATCCAGCAGGTTGAAACTACTCAGAGAAAG ATTCAGGAAAATCAGCCTGAAAATAGTAAAACCCTAGCCACACAGTTGAATCAACAGAAGATGCTGGTG TCCGAAATAGAAATGAAACAGAGCAAAATGGACGAGTGTCAAAAATATGCAGAACAGTACTCAGCTACA GTGAAGGACTATGAATTACAAACAATGACCTACCGGGCCATGGTAGATTCACAACAAAAATCTCCAGTG AAACGCCGAAGAATGCAGAGTTCAGCAGATCTCATTATTCAAGAGTTCATGGACCTAAGGACTCGATAT ACTGCCCTGGTCACTCTCATGACACAATATATTAAATTTGCTGGTGATTCATTGAAGAGGCTGGAAGAG GAGGAGATTAAAAGGTGTAAGGAGACTTCTGAACATGGGGCATATTCAGATCTGCTTCAGCGTCAGAAG GCAACAGTGCTTGAGAATAGCAAACTTACAGGAAAGATAAGTGAGTTGGAAAGAATGGTAGCTGAACTA AAGAAACAAAAGTCCCGAGTAGAGGAAGAACTTCCGAAGGTCAGGGAGGCTGCAGAAAATGAATTGAGA AAGCAGCAGAGAAATGTAGAAGATATCTCTCTGCAGAAGATAAGGGCTGAAAGTGAAGCCAAGCAGTAC CGCAGGGAACTTGAAACCATTGTGAGAGAGAAGGAAGCCGCTGAAAGAGAACTGGAGCGGGTGAGGCAG CTCACCATAGAGGCCGAGGCTAAAAGAGCTGCCGTGGAAGAGAACCTCCTGAATTTTCGCAATCAGTTG GAGGAAAACACCTTTACCAGACGAACACTGGAAGATCATCTTAAAAGAAAAGATTTAAGTCTCAATGAT TTGGAGCAACAAAAAAATAAATTAATGGAAGAATTAAGAAGAAAGAGAGACAATGAGGAAGAACTCTTG AAGCTGATAAAGCAGATGGAAAAAGACCTTGCATTTCAGAAACAGGTAGCAGAGAAACAGTTGAAAGAA AAGCAGAAAATTGAATTGGAAGCAAGAAGAAAAATAACTGAAATTCAGTATACATGTAGAGAAAATGCA TTGCCAGTGTGTCCGATCACACAGGCTACATCATGCAGGGCAGTAACGGGTCTCCAGCAAGAACATGAC AAGCAGAAAGCAGAAGAACTCAAACAGCAGGTAGATGAACTAACAGCTGCCAATAGAAAGGCTGAACAA GACATGAGAGAGCTGACATATGAACTTAATGCCCTCCAGCTTGAAAAAACGTCATCTGAGGAAAAGGCT CGTTTGCTAAAAGATAAACTAGATGAAACAAATAATACACTCAGATGCCTTAAGTTGGAGCTGGAAAGG AAGGATCAGGCGGAGAAAGGGTATTCTCAACAACTCAGAGAGCTTGGTAGGCAATTGAATCAAACCACA GGTAAAGCTGAAGAAGCCATGCAAGAAGCTAGTGATCTCAAGAAAATAAAGCGCAATTATCAGTTAGAA TTAGAATCTCTTAATCATGAAAAAGGGAAACTACAAAGAGAAGTAGACAGAATCACAAGGGCACATGCT GTAGCTGAGAAGAATATTCAGCATTTAAATTCACAAATTCATTCTTTTCGAGATGAGAAAGAATTAGAA AGACTACAAATCTGCCAGAGAAAATCAGATCATCTAAAAGAACAATTTGAGAAAAGCCATGAGCAGTTG CTTCAAAATATCAAAGCTGAAAAAGAAAATAATGATAAAATCCAAAGGCTCAATGAAGAATTGGAGAAA AGTAATGAGTGTGCAGAGATGCTAAAACAAAAAGTAGAGGAGCTTACTAGGCAGAATAATGAAACCAAA TTAATGATGCAGAGAATTCAGGCAGAATCAGAGAATATAGTTTTAGAGAAACAAACTATCCAGCAAAGA TGTGAAGCACTGAAAATTCAGGCAGATGGTTTTAAAGATCAGCTACGCAGCACAAATGAACACTTGCAT AAACAGACAAAAACAGAGCAGGATTTTCAAAGAAAAATTAAATGCCTAGAAGAAGACCTGGCGAAAAGT CAAAATTTGGTAAGTGAATTTAAGCAAAAGTGTGACCAACAGAACATTATCATCCAGAATACCAAGAAA GAAGTTAGAAATCTGAATGCGGAACTGAATGCTTCCAAAGAAGAGAAGCGACGCGGGGAGCAGAAAGTT CAGCTACAACAAGCTCAGGTGCAAGAGTTAAATAACAGGTTGAAAAAAGTACAAGACGAATTACACTTA AAGACCATAGAGGAGCAGATGACCCACAGAAAGATGGTTCTGTTTCAGGAAGAATCTGGTAAATTCAAA CAATCAGCAGAGGAGTTTCGGAAGAAGATGGAAAAATTAATGGAGTCCAAAGTCATCACTGAAAATGAT ATTTCAGGCATTAGGCTTGACTTTGTGTCTCTTCAACAAGAAAACTCTAGAGCCCAAGAAAATGCTAAG CTTTGTGAAACAAACATTAAAGAACTTGAAAGACAGCTTCAACAGTATCGTGAACAAATGCAGCAAGGG CAGCACATGGAAGCAAATCATTACCAAAAATGTCAGAAACTTGAGGATGAGCTGATAGCCCAGAAGCGT GAGGTTGAAAACCTGAAGCAAAAAATGGACCAACAGATCAAAGAGCATGAACATCAATTAGTTTTGCTC CAGTGTGAAATTCAAAAAAAGAGCACAGCCAAAGACTGTACCTTCAAACCAGATTTTGAGATGACAGTG AAGGAGTGCCAGCACTCTGGAGAGCTGTCCTCTAGAAACACTGGACACCTTCACCCAACACCCAGATCC CCTCTGTTGAGATGGACTCAAGAACCACAGCCATTGGAAGAGAAGTGGCAGCATCGGGTTGTTGAACAG ATACCCAAAGAAGTCCAATTCCAGCCACCAGGGGCTCCACTCGAGAAAGAGAAAAGCCAGCAGTGTTAC TCTGAGTACTTTTCTCAGACAAGCACCGAGTTACAGATAACTTTTGATGAGACAAACCCCATTACAAGA CTGTCTGAAATTGAGAAGATAAGAGACCAAGCCCTGAACAATTCTAGACCACCTGTTAGGTATCAAGAT AACGCATGTGAAATGGAACTGGTGAAGGTTTTGACACCCTTAGAGATAGCTAAGAACAAGCAGTATGAT ATGCATACAGAAGTCACAACATTAAAACAAGAAAAGAACCCAGTTCCCAGTGCTGAAGAATGGATGCTT GAAGGGTGCAGAGCATCTGGTGGACTCAAGAAAGGGGATTTCCTTAAGAAGGGCTTAGAACCAGAGACC TTCCAGAACTTTGATGGTGATCATGCATGTTCAGTCAGGGATGATGAATTTAAATTCCAAGGGCTTAGG CACACTGTGACTGCCAGGCAGTTGGTGGAAGCTAAGCTTCTGGACATGAGAACAATTGAGCAGCTGCGA CTCGGTCTTAAGACTGTTGAAGAAGTTCAGAAAACTCTTAACAAGTTTCTGACGAAAGCCACCTCAATT GCAGGGCTTTACCTAGAATCTACAAAAGAAAAGATTTCATTTGCCTCAGCGGCCGAGAGAATCATAATA GACAAAATGGTGGCTTTGGCATTTTTAGAAGCTCAGGCTGCAACAGGTTTTATAATTGATCCCATTTCA GGTCAGACATATTCTGTTGAAGATGCAGTTCTTAAAGGAGTTGTTGACCCCGAATTCAGAATTAGGCTT CTTGAGGCAGAGAAGGCAGCTGTGGGATATTCTTATTCTTCTAAGACATTGTCAGTGTTTCAAGCTATG GAAAATAGAATGCTTGACAGACAAAAAGGTAAACATATCTTGGAAGCCCAGATTGCCAGTGGGGGTGTC ATTGACCCTGTGAGAGGCATTCGTGTTCCTCCAGAAATTGCTCTGCAGCAGGGGTTGTTGAATAATGCC ATCTTACAGTTTTTACATGAGCCATCCAGCAACACAAGAGTTTTCCCTAATCCCAATAACAAGCAAGCT CTGTATTACTCAGAATTACTGCGAATGTGTGTATTTGATGTAGAGTCCCAATGCTTTCTGTTTCCATTT GGGGAGAGGAACATTTCCAATCTCAATGTCAAGAAAACACATAGAATTTCTGTAGTAGATACTAAAACA GGATCAGAATTGACCGTGTATGAGGCTTTCCAGAGAAACCTGATTGAGAAAAGTATATATCTTGAACTT TCAGGGCAGCAATATCAGTGGAAGGAAGCTATGTTTTTTGAATCCTATGGGCATTCTTCTCATATGCTG ACTGATACTAAAACAGGATTACACTTCAATATTAATGAGGCTATAGAGCAGGGAACAATTGACAAAGCC TTGGTCAAAAAGTATCAGGAAGGCCTCATCACACTTACAGAACTTGCTGATTCTTTGCTGAGCCGGTTA GTCCCCAAGAAAGATTTGCACAGTCCTGTTGCAGGGTATTGGCTGACTGCTAGTGGGGAAAGGATCTCT GTACTAAAAGCCTCCCGTAGAAATTTGGTTGATCGGATTACTGCCCTCCGATGCCTTGAAGCCCAAGTC AGTACAGGGGGCATAATTGATCCTCTTACTGGCAAAAAGTACCGGGTGGCCGAAGCTTTGCATAGAGGC CTGGTTGATGAGGGGTTTGCCCAGCAGCTGCGACAGTGTGAATTAGTAATCACAGGGATTGGCCATCCC ATCACTAACAAAATGATGTCAGTGGTGGAAGCTGTGAATGCAAATATTATAAATAAGGAAATGGGAATC CGATGTTTGGAATTTCAGTACTTGACAGGAGGGTTGATAGAGCCACAGGTTCACTCTCGGTTATCAATA GAAGAGGCTCTCCAAGTAGGTATTATAGATGTCCTCATTGCCACAAAACTCAAAGATCAAAAGTCATAT GTCAGAAATATAATATGCCCTCAGACAAAAAGAAAGTTGACATATAAAGAAGCCTTAGAAAAAGCTGAT TTTGATTTCCACACAGGACTTAAACTGTTAGAAGTATCTGAGCCCCTGATGACAGGAATTTCTAGCCTC TACTATTCTTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001723 |
Insert Size | 7950 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001723.5 |
RefSeq Size | 9006 bp |
RefSeq ORF | 7950 bp |
Locus ID | 667 |
UniProt ID | Q03001 |
MW | 306.8 kDa |
Gene Summary | This gene encodes a member of the plakin protein family of adhesion junction plaque proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the full-length nature of some variants has not been defined. It has been reported that some isoforms are expressed in neural and muscle tissue, anchoring neural intermediate filaments to the actin cytoskeleton, and some isoforms are expressed in epithelial tissue, anchoring keratin-containing intermediate filaments to hemidesmosomes. Consistent with the expression, mice defective for this gene show skin blistering and neurodegeneration. [provided by RefSeq, Mar 2010] Transcript Variant: This variant (1e) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 2. This variant also differs in the 3' coding region and 3' UTR, compared to variant 2. The resulting isoform (1e) has shorter and distinct N- and C-termini compared to isoform 2. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214007 | DST (Myc-DDK-tagged)-Human dystonin (DST), transcript variant 1e |
CNY 26,232.00 |
|
RC214007L1 | Lenti ORF clone of Human dystonin (DST), transcript variant 1e, Myc-DDK-tagged |
CNY 20,890.00 |
|
RG214007 | DST (tGFP-tagged) - Human dystonin (DST), transcript variant 1e |
CNY 27,832.00 |
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