NKCC1 (SLC12A2) (NM_001046) Human Untagged Clone
CAT#: SC302962
SLC12A2 (untagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2)
CNY 12,664.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | BSC; BSC2; KILQS; NKCC1; PPP1R141 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_001046 edited
GGGGGCCTCTGTGGCCGTCCAGGCTAGCGGCGGCCCGCAGGCGGCGGGGAGAAAGACTCT CTCACCTGGTCTTGCGGCTGTGGCCACCGCCGGCCAGGGGTGTGGAGGGCGTGCTGCCGG AGACGTCCGCCGGGCTCTGCAGTTCCGCCGGGGGTCGGGCAGCTATGGAGCCGCGGCCCA CGGCGCCCTCCTCCGGCGCCCCGGGACTGGCCGGGGTCGGGGAGACGCCGTCAGCCGCTG CGCTGGCCGCAGCCAGGGTGGAACTGCCCGGCACGGCTGTGCCCTCGGTGCCGGAGGATG CTGCGCCCGCGAGCCGGGACGGCGGCGGGGTCCGCGATGAGGGCCCCGCGGCGGCCGGGG ACGGGCTGGGCAGACCCTTGGGGCCCACCCCGAGCCAGAGCCGTTTCCAGGTGGACCTGG TTTCCGAGAACGCCGGGCGGGCCGCTGCTGCGGCGGCGGCGGCGGCGGCGGCAGCGGCGG CGGCTGGTGCTGGGGCGGGGGCCAAGCAGACCCCCGCGGACGGGGAAGCCAGCGGCGAGA GCGAGCCGGTTAAAGGCAGCGAGGAAGCCAAGGGCCGCTTCCGCGTGAACTTCGTGGACC CAGCTGCCTCCTCGTCGGCTGAAGACAGCCTGTCAGATGCTGCCGGGGTCGGAGTCGACG GGCCCAACGTGAGCTTCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGC ACTCCGGCGGCGGCGGCGGCAGTGGGCACCACCAGCACTACTATTATGATACCCACACCA ACACCTACTACCTGCGCACCTTCGGCCACAACACCATGGACGCTGTGCCCAGGATCGATC ACTACCGGCACACAGCCGCGCAGCTGGGCGAGAAGCTGCTCCGGCCTAGCCTGGCGGAGC TCCACGACGAGCTGGAAAAGGAACCTTTTGAGGATGGCTTTGCAAATGGGGAAGAAAGTA CTCCAACCAGAGATGCTGTGGTCACGTATACTGCAGAAAGTAAAGGAGTCGTGAAGTTTG GCTGGATCAAGGGTGTATTAGTACGTTGTATGTTAAACATTTGGGGTGTGATGCTTTTCA TTAGATTGTCATGGATTGTGGGTCAAGCTGGAATAGGTCTATCAGTCCTTGTAATAATGA TGGCCACTGTTGTGACAACTATCACAGGATTGTCTACTTCAGCAATAGCAACTAATGGAT TTGTAAGAGGAGGAGGAGCATATTATTTAATATCTAGAAGTCTAGGGCCAGAATTTGGTG GTGCAATTGGTCTAATCTTCGCCTTTGCCAACGCTGTTGCAGTTGCTATGTATGTGGTTG GATTTGCAGAAACCGTGGTGGAGTTGCTTAAGGAACATTCCATACTTATGATAGATGAAA TCAATGATATCCGAATTATTGGAGCCATTACAGTCGTGATTCTTTTAGGTATCTCAGTAG CTGGAATGGAGTGGGAAGCAAAAGCTCAGATTGTTCTTTTGGTGATCCTACTTCTTGCTA TTGGTGATTTCGTCATAGGAACATTTATCCCACTGGAGAGCAAGAAGCCAAAAGGGTTTT TTGGTTATAAATCTGAAATATTTAATGAGAACTTTGGGCCCGATTTTCGAGAGGAAGAGA CTTTCTTTTCTGTATTTGCCATCTTTTTTCCTGCTGCAACTGGTATTCTGGCTGGAGCAA ATATCTCAGGTGATCTTGCAGATCCTCAGTCAGCCATACCCAAAGGAACACTCCTAGCCA TTTTAATTACTACATTGGTTTACGTAGGAATTGCAGTATCTGTAGGTTCTTGTGTTGTTC GAGATGCCACTGGAAACGTTAATGACACTATCGTAACAGAGCTAACAAACTGTACTTCTG CAGCCTGCAAATTAAACTTTGATTTTTCATCTTGTGAAAGCAGTCCTTGTTCCTATGGCC TAATGAACAACTTCCAGGTAATGAGTATGGTGTCAGGATTTACACCACTAATTTCTGCAG GTATATTTTCAGCCACTCTTTCTTCAGCATTAGCATCCCTAGTGAGTGCTCCCAAAATAT TTCAGGCTCTATGTAAGGACAACATCTACCCAGCTTTCCAGATGTTTGCTAAAGGTTATG GGAAAAATAATGAACCTCTTCGTGGCTACATCTTAACATTCTTAATTGCACTTGGATTCA TCTTAATTGCTGAACTGAATGTTATTGCACCAATTATCTCAAACTTCTTCCTTGCATCAT ATGCATTGATCAATTTTTCAGTATTCCATGCATCACTTGCAAAATCTCCAGGATGGCGTC CTGCATTCAAATACTACAACATGTGGATATCACTTCTTGGAGCAATTCTTTGTTGCATAG TAATGTTCGTCATTAACTGGTGGGCTGCATTGCTAACATATGTGATAGTCCTTGGGCTGT ATATTTATGTTACCTACAAAAAACCAGATGTGAATTGGGGATCCTCTACACAAGCCCTGA CTTACCTGAATGCACTGCAGCATTCAATTCGTCTTTCTGGAGTGGAAGACCACGTGAAAA ACTTTAGGCCACAGTGTCTTGTTATGACAGGTGCTCCAAACTCACGTCCAGCTTTACTTC ATCTTGTTCATGATTTCACAAAAAATGTTGGTTTGATGATCTGTGGCCATGTACATATGG GTCCTCGAAGACAAGCCATGAAAGAGATGTCCATCGATCAAGCCAAATATCAGCGATGGC TTATTAAGAACAAAATGAAGGCATTTTATGCTCCAGTACATGCAGATGACTTGAGAGAAG GTGCACAGTATTTGATGCAGGCTGCTGGTCTTGGTCGTATGAAGCCAAACACACTTGTCC TTGGATTTAAGAAAGATTGGTTGCAAGCAGATATGAGGGATGTGGATATGTATATAAACT TATTTCATGATGCTTTTGACATACAATATGGAGTAGTGGTTATTCGCCTAAAAGAAGGTC TGGATATATCTCATCTTCAAGGACAAGAAGAATTATTGTCATCACAAGAGAAATCTCCTG GCACCAAGGATGTGGTAGTAAGTGTGGAATATAGTAAAAAGTCCGATTTAGATACTTCCA AACCACTCAGTGAAAAACCAATTACACACAAAGTTGAGGAAGAGGATGGCAAGACTGCAA CTCAACCACTGTTGAAAAAAGAATCCAAAGGCCCTATTGTGCCTTTAAATGTAGCTGACC AAAAGCTTCTTGAAGCTAGTACACAGTTTCAGAAAAAACAAGGAAAGAATACTATTGATG TCTGGTGGCTTTTTGATGATGGAGGTTTGACCTTATTGATACCTTACCTTCTGACGACCA AGAAAAAATGGAAAGACTGTAAGATCAGAGTATTCATTGGTGGAAAGATAAACAGAATAG ACCATGACCGGAGAGCGATGGCTACTTTGCTTAGCAAGTTCCGGATAGACTTTTCTGATA TCATGGTTCTAGGAGATATCAATACCAAACCAAAGAAAGAAAATATTATAGCTTTTGAGG AAATCATTGAGCCATACAGACTTCATGAAGATGATAAAGAGCAAGATATTGCAGATAAAA TGAAAGAAGATGAACCATGGCGAATAACAGATAATGAGCTTGAACTTTATAAGACCAAGA CATACCGGCAGATCAGGTTAAATGAGTTATTAAAGGAACATTCAAGCACAGCTAATATTA TTGTCATGAGTCTCCCAGTTGCACGAAAAGGTGCTGTGTCTAGTGCTCTCTACATGGCAT GGTTAGAAGCTCTATCTAAGGACCTACCACCAATCCTCCTAGTTCGTGGGAATCATCAGA GTGTCCTTACCTTCTATTCATAAATGTTCTATACAGTGGACAGCCCTCCAGAATGGTACT TCAGTGCCTAGTGTAGTAACTGAAATCTTCAATGACACATTAACATCACAATGGCGAATG GTGACTTTTCTTTCACGATTTCATTAATTTGAAAGCACACAGGAAAGTTGCTCCATTGAT AACGTGTATGGAGACTTCGGTTTTAGTCAATTCCATATCTCAATCTTAATGGTGATTCTT CTCTGTTGAACTGAAGTTTGTGAGAGTAGTTTTCCTTTGCTACTTGAATAGCAATAAAAG CGTGTTAACTTTTTGATTGATGAAAGAAGTACAAAAAGCCTTTAGCCTTGAGGTGCCTTC TGAAATTAACCAAATTTCATCCATATATCCTCTTTTATAAACTTATAGAATGTCAAAAAA AAAAAAAAAA |
Restriction Sites | Please inquire |
ACCN | NM_001046 |
Insert Size | 4200 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_001046.2. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001046.2, NP_001037.1 |
RefSeq Size | 6891 bp |
RefSeq ORF | 3639 bp |
Locus ID | 6558 |
UniProt ID | P55011 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Vibrio cholerae infection |
Gene Summary | The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224053 | SLC12A2 (Myc-DDK-tagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2) |
CNY 12,656.00 |
|
RC224053L3 | Lenti ORF clone of Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2), Myc-DDK-tagged |
CNY 13,970.00 |
|
RC224053L4 | Lenti ORF clone of Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2), mGFP tagged |
CNY 15,056.00 |
|
RG224053 | SLC12A2 (tGFP-tagged) - Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2) |
CNY 14,256.00 |