NKCC1 (SLC12A2) (NM_001046) Human Tagged Lenti ORF Clone
CAT#: RC224053L4
- LentiORF®
Lenti ORF clone of Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2), mGFP tagged
"NM_001046" in other vectors (4)
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CNY 15,056.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Synonyms | BSC; BSC2; KILQS; NKCC1; PPP1R141 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC224053).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001046 |
ORF Size | 3636 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001046.2 |
RefSeq Size | 6891 bp |
RefSeq ORF | 3639 bp |
Locus ID | 6558 |
UniProt ID | P55011 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Vibrio cholerae infection |
MW | 131.3 kDa |
Gene Summary | The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224053 | SLC12A2 (Myc-DDK-tagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2) |
CNY 12,656.00 |
|
RC224053L3 | Lenti ORF clone of Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2), Myc-DDK-tagged |
CNY 13,970.00 |
|
RG224053 | SLC12A2 (tGFP-tagged) - Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2) |
CNY 14,256.00 |
|
SC302962 | SLC12A2 (untagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2) |
CNY 12,664.00 |