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GPR149 (NM_001038705) Human Untagged Clone
CAT＃: SC302956
GPR149 (untagged)-Human G protein-coupled receptor 149 (GPR149)
CNY 8,040.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IEDA; PGR10; R35 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_001038705 edited
ATGTCTTTATTTCTCAGTAACTTATCAACAAATGACTCTAGCCTGTGGAAAGAGAATCAT AATTCTACGGACCTTTTAAATCCGCCAGGAACCCTGAATATCTATCTTTTTTGCTTGACA TGTCTCATGACTTTTGCAGCCTTGGTGGGCAGCATTTATTCACTAATTTCCCTGCTGAAA ATGCAGAACAGAACTGTTGTGTCCATGCTTGTGGCTTCCTGGTCTGTGGATGATCTCATG AGCGTCCTGTCGGTGACCATCTTCATGTTTTTGCAGTGGCCAAACGAGGTCCCCGGTTAC TTCCAATTTCTGTGCACCACCTCTGCCTTAATGTATTTATGCCAGGGCCTCTCTAGCAAC TTGAAGGCGACTCTCCTAGTCTCTTACAACTTTTATACGATGCACAGAGGTGTGGGGAGC CAGACAGCCTCCAGAAGATCGGGCCAGGTGCTCGGCGTGGTGCTGACCGTGTGGGCAGCC AGTCTGCTGCTCTCGGCGCTCCCGCTGTGCGGCTGGGGCGCCTTCGTGCGCACGCCCTGG GGCTGCCTGGTGGACTGCTCCAGCTCCTACGTACTATTCCTCTCTATCGTGTACGCTTTG GCCTTCGGACTCCTCGTGGGCCTCTCAGTCCCACTCACTCACCGATTGCTGTGTTCGGAG GAGCCGCCGAGACTCCACTCCAACTACCAGGAAATTTCCCGTGGAGCTTCAATTCCTGGG ACCCCTCCTACTGCGGGGAGAGTGGTTTCCCTGTCCCCAGAGGATGCTCCAGGCCCGAGT CTGCGGCGCTCTGGGGGATGCTCTCCGAGCTCCGACACCGTGTTCGGACCGGGTGCGCCC GCTGCCGCTGGGGCTGAAGCCTGCAGGCGTGAGAACCGGGGGACTCTCTATGGCACCAGG AGCTTCACCGTGAGCGTAGCGCAGAAGCGCTTCGCTTTGATCCTAGCGCTTACAAAAGTC GTCCTTTGGCTGCCCATGATGATGCACATGGTGGTCCAGAACGTCGTGGGGTTTCAGAGC CTTCCCTTGGAGACATTCAGCTTTCTACTTACCCTGCTGGCCACCACTGTAACCCCAGTG TTTGTCTTGTCCAAACGCTGGACCCACTTGCCCTGTGGCTGCATCATCAACTGCAGGCAG AACGCATATGCAGTGGCGTCCGATGGGAAAAAAATCAAGAGAAAAGGCTTTGAATTCAAT CTATCATTCCAAAAAAGTTATGGGATTTATAAAATAGCACATGAAGATTACTATGATGAT GATGAAAATTCCATATTCTATCACAACCTGATGAACTCTGAGTGTGAAACTACAAAAGAC CCTCAGAGAGACAACCGTAACATCTTCAATGCTATAAAAGTAGAAATCAGCACCACGCCC TCTCTGGACAGCTCCACACAAAGAGGCATCAACAAATGCACAAATACTGATATTACAGAA GCTAAACAGGATTCCAACAACAAAAAGGATGCGTTTTCTGACAAAACAGGAGGTGATATT AACTATGAAGAAACTACCTTTTCTGAAGGGCCAGAAAGAAGACTGTCTCATGAAGAGAGT CAGAAACCAGATCTTTCAGACTGGGAGTGGTGTAGGAGTAAATCAGAAAGAACCCCTCGT CAGCGTTCCGGTTATGCCCTTGCCATTCCCTTGTGTGCATTCCAGGGGACTGTGTCTCTC CATGCACCTACAGGGAAAACCCTATCTCTTTCTACCTATGAGGTAAGCGCAGAAGGGCAA AAAATAACTCCAGCCTCTAAGAAAATAGAAGTCTATCGATCCAAAAGTGTTGGCCATGAA CCAAACTCAGAAGATTCTTCATCCACGTTTGTGGACACCAGTGTGAAAATACACTTGGAG GTTCTTGAAATTTGTGATAATGAAGAGGCCTTGGACACTGTGTCAATCATTAGTAACATC AGTCAGTCCTCCACACAAGTCAGATCTCCATCCCTACGTTACTCCAGGAAAGAAAACAGA TTTGTTTCATGTGACCTAGGGGAAACAGCCTCATACTCCCTCTTTTTGCCCACCAGTAAT CCTGATGGTGATATTAATATCTCCATTCCAGACACAGTAGAAGCACACAGGCAGAACAGT AAAAGGCAGCATCAAGAGAGGGATGGCTACCAGGAGGAAATCCAGTTGTTAAATAAAGCT TACAGAAAAAGAGAGGAAGAAAGCAAGGGTAGTTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_001038705 |
| Insert Size | 5200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. It has a 3kb 3' UTR sequence not found in the RefSeq sequence. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001038705.1, NP_001033794.1 |
| RefSeq Size | 2323 bp |
| RefSeq ORF | 2196 bp |
| Locus ID | 344758 |
| UniProt ID | Q86SP6 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | This gene encodes a seven-transmembrane G protein coupled receptor (GPCR) class A family member. Although categorized as a class A GPCR, the encoded protein lacks the first two charged amino acids of the highly conserved Asp-Arg-Tyr (DRY) motif found in the third transmembrane helix of class A receptors which is important for efficient G protein-coupled signal transduction. Mice with a knockout of the orthologous gene are viable and have normal maturation of the ovarian follicle, but show enhanced fertility and ovulation. All GPCRs have a common structural architecture consisting of seven transmembrane alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptor, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits. The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. [provided by RefSeq, Jul 2017] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223772 | GPR149 (Myc-DDK-tagged)-Human G protein-coupled receptor 149 (GPR149) |
CNY 8,032.00 |
|
| RC223772L1 | Lenti ORF clone of Human G protein-coupled receptor 149 (GPR149), Myc-DDK-tagged |
CNY 10,432.00 |
|
| RC223772L2 | Lenti ORF clone of Human G protein-coupled receptor 149 (GPR149), mGFP tagged |
CNY 9,600.00 |
|
| RC223772L3 | Lenti ORF clone of Human G protein-coupled receptor 149 (GPR149), Myc-DDK-tagged |
CNY 9,600.00 |
|
| RC223772L4 | Lenti ORF clone of Human G protein-coupled receptor 149 (GPR149), mGFP tagged |
CNY 9,600.00 |
|
| RG223772 | GPR149 (tGFP-tagged) - Human G protein-coupled receptor 149 (GPR149) |
CNY 8,460.00 |
