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FBXW7 (NM_001013415) Human Untagged Clone
CAT＃: SC301782
FBXW7 (untagged)-Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 3
CNY 9,600.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC301782 representing NM_001013415.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCAAAACCGGGAAAACCTACTCTAAACCATGGCTTGGTTCCTGTTGATCTTAAAAGTGCAAAAGAG CCTCTACCACATCAAACTGTGATGAAGATATTTAGCATTAGCATCATTGCCCAAGGCCTCCCTTTTTGT CGAAGACGGATGAAAAGAAAGTTGGACCATGGTTCTGAGGTCCGCTCTTTTTCTTTGGGAAAGAAACCA TGCAAAGTCTCAGAATATACAAGTACCACTGGGCTTGTACCATGTTCAGCAACACCAACAACTTTTGGG GACCTCAGAGCAGCCAATGGCCAAGGGCAACAACGACGCCGAATTACATCTGTCCAGCCACCTACAGGC CTCCAGGAATGGCTAAAAATGTTTCAGAGCTGGAGTGGACCAGAGAAATTGCTTGCTTTAGATGAACTC ATTGATAGTTGTGAACCAACACAAGTAAAACATATGATGCAAGTGATAGAACCCCAGTTTCAACGAGAC TTCATTTCATTGCTCCCTAAAGAGTTGGCACTCTATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTA CAAGCAGCTCAGACATGTCGCTACTGGAGAATTTTGGCTGAAGACAACCTTCTCTGGAGAGAGAAATGC AAAGAAGAGGGGATTGATGAACCATTGCACATCAAGAGAAGAAAAGTAATAAAACCAGGTTTCATACAC AGTCCATGGAAAAGTGCATACATCAGACAGCACAGAATTGATACTAACTGGAGGCGAGGAGAACTCAAA TCTCCTAAGGTGCTGAAAGGACATGATGATCATGTGATCACATGCTTACAGTTTTGTGGTAACCGAATA GTTAGTGGTTCTGATGACAACACTTTAAAAGTTTGGTCAGCAGTCACAGGCAAATGTCTGAGAACATTA GTGGGACATACAGGTGGAGTATGGTCATCACAAATGAGAGACAACATCATCATTAGTGGATCTACAGAT CGGACACTCAAAGTGTGGAATGCAGAGACTGGAGAATGTATACACACCTTATATGGGCATACTTCCACT GTGCGTTGTATGCATCTTCATGAAAAAAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGG GATATTGAGACAGGCCAGTGTTTACATGTTTTGATGGGTCATGTTGCAGCAGTCCGCTGTGTTCAATAT GATGGCAGGAGGGTTGTTAGTGGAGCATATGATTTTATGGTAAAGGTGTGGGATCCAGAGACTGAAACC TGTCTACACACGTTGCAGGGGCATACTAATAGAGTCTATTCATTACAGTTTGATGGTATCCATGTGGTG AGTGGATCTCTTGATACATCAATCCGTGTTTGGGATGTGGAGACAGGGAATTGCATTCACACGTTAACA GGGCACCAGTCGTTAACAAGTGGAATGGAACTCAAAGACAATATTCTTGTCTCTGGGAATGCAGATTCT ACAGTTAAAATCTGGGATATCAAAACAGGACAGTGTTTACAAACATTGCAAGGTCCCAACAAGCATCAG AGTGCTGTGACCTGTTTACAGTTCAACAAGAACTTTGTAATTACCAGCTCAGATGATGGAACTGTAAAA CTATGGGACTTGAAAACGGGTGAATTTATTCGAAACCTAGTCACATTGGAGAGTGGGGGGAGTGGGGGA GTTGTGTGGCGGATCAGAGCCTCAAACACAAAGCTGGTGTGTGCAGTTGGGAGTCGGAATGGGACTGAA GAAACCAAGCTGCTGGTGCTGGACTTTGATGTGGACATGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001013415 |
| Insert Size | 1770 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001013415.1 |
| RefSeq Size | 3570 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 55294 |
| UniProt ID | Q969H0 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 66.1 kDa |
| Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (3) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (3) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221116 | FBXW7 (Myc-DDK-tagged)-Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 3 |
CNY 4,384.00 |
|
| RC221116L1 | Lenti ORF clone of Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 3, Myc-DDK-tagged |
CNY 6,784.00 |
|
| RC221116L2 | Lenti ORF clone of Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 3, mGFP tagged |
CNY 7,130.00 |
|
| RC221116L3 | Lenti ORF clone of Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 3, Myc-DDK-tagged |
CNY 7,130.00 |
|
| RC221116L4 | Lenti ORF clone of Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 3, mGFP tagged |
CNY 6,784.00 |
|
| RG221116 | FBXW7 (tGFP-tagged) - Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 3 |
CNY 5,700.00 |
