MAGED1 (NM_001005332) Human Untagged Clone

CAT#: SC300905

MAGED1 (untagged)-Human melanoma antigen family D, 1 (MAGED1), transcript variant 3



  "NM_001005332" in other vectors (4)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Anti-MAGED1 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DLXIN-1; NRAGE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC300905 representing NM_001005332.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCTCAGAAAATGGACTGTGGTGCGGGCCTCCTCGGCTTCCAGGCTGAGGCCTCCGTAGAAGACAGC
GCCTTGCTTATGCAGACCTTGATGGAGGCCATCCAGATCTCAGAGGCTCCACCTACTAACCAGGCCACC
GCAGCTGCTAGTCCCCAGAGTTCACAGCCCCCAACTGCCAATGAGATGGCTGACATTCAGGTTTCAGCA
GCTGCCGCTAGGCCTAAGTCAGCCTTTAAAGTCCAGAATGCCACCACAAAAGGCCCAAATGGTGTCTAT
GATTTCTCTCAGGCTCATAATGCCAAGGATGTGCCCAACACGCAGCCCAAGGCAGCCTTTAAGTCCCAA
AATGCTACCCCAAAGGGTCCAAATGCTGCCTATGATTTTTCCCAGGCAGCAACCACTGGTGAGTTAGCT
GCTAACAAGTCTGAGATGGCCTTCAAGGCCCAGAATGCCACTACTAAAGTGGGCCCAAATGCCACCTAC
AATTTCTCTCAGTCTCTCAATGCCAATGACCTGGCCAACAGCAGGCCTAAGACCCCTTTCAAGGCTTGG
AATGATACCACTAAGGCCCCAACAGCTGATACCCAGACCCAGAATGTAAATCAGGCCAAAATGGCCACT
TCCCAGGCTGACATAGAGACCGACCCAGGTATCTCTGAACCTGACGGTGCAACTGCACAGACATCAGCA
GATGGTTCCCAGGCTCAGAATCTGGAGTCCCGGACAATAATTCGGGGCAAGAGGACCCGCAAGATTAAT
AACTTGAATGTTGAAGAGAACAGCAGTGGGGATCAGAGGCGGGCCCCACTGGCTGCAGGGACCTGGAGG
TCTGCACCAGTTCCAGTGACCACTCAGAACCCACCTGGCGCACCCCCCAATGTGCTCTGGCAGACGCCA
TTGGCTTGGCAGAACCCCTCAGGCTGGCAAAACCAGACAGCCAGGCAGACCCCACCAGCACGTCAGAGC
CCTCCAGCTAGGCAGACCCCACCAGCCTGGCAGAACCCAGTCGCTTGGCAGAACCCAGTGATTTGGCCA
AACCCAGTAATCTGGCAGAACCCAGTGATCTGGCCAAACCCCATTGTCTGGCCCGGCCCTGTTGTCTGG
CCGAATCCACTGGCCTGGCAGAATCCACCTGGATGGCAGACTCCACCTGGATGGCAGACCCCACCGGGC
TGGCAGGGTCCTCCAGACTGGCAAGGTCCTCCTGACTGGCCGCTACCACCCGACTGGCCACTGCCACCT
GATTGGCCACTTCCCACTGACTGGCCACTACCACCTGACTGGATCCCCGCTGATTGGCCAATTCCACCT
GACTGGCAGAACCTGCGCCCCTCGCCTAACCTGCGCCCTTCTCCCAACTCGCGTGCCTCACAGAACCCA
GGTGCTGCACAGCCCCGAGATGTGGCCCTTCTTCAGGAAAGAGCAAATAAGTTGGTCAAGTACTTGATG
CTTAAGGACTACACAAAGGTGCCCATCAAGCGCTCAGAAATGCTGAGAGATATCATCCGTGAATACACT
GATGTTTATCCAGAAATCATTGAACGTGCATGCTTTGTCCTAGAGAAGAAATTTGGGATTCAACTGAAA
GAAATTGACAAAGAAGAACACCTGTATATTCTCATCAGTACCCCCGAGTCCCTGGCTGGCATACTGGGA
ACGACCAAAGACACACCCAAGCTCGGTCTCCTCTTGGTGATTCTGGGTGTCATCTTCATGAATGGCAAC
CGTGCCAGTGAGGCTGTCCTCTGGGAGGCACTACGCAAGATGGGACTGCGTCCTGGGGTGAGACATCCC
CTCCTTGGAGATCTAAGGAAACTTCTCACCTATGAGTTTGTAAAGCAGAAATACCTGGACTACAGACGA
GTGCCCAACAGCAACCCCCCGGAGTATGAGTTCCTCTGGGGCCTCCGTTCCTACCATGAGACTAGCAAG
ATGAAAGTGCTGAGATTCATTGCAGAGGTTCAGAAAAGAGACCCTCGTGACTGGACTGCACAGTTCATG
GAGGCTGCAGATGAGGCCTTGGATGCTCTGGATGCTGCTGCAGCTGAGGCCGAAGCCCGGGCTGAAGCA
AGAACCCGCATGGGAATTGGAGATGAGGCTGTGTCTGGGCCCTGGAGCTGGGATGACATTGAGTTTGAG
CTGCTGACCTGGGATGAGGAAGGAGATTTTGGAGATCCCTGGTCCAGAATTCCATTTACCTTCTGGGCC
AGATACCACCAGAATGCCCGCTCCAGATTCCCTCAGACCTTTGCCGGTCCCATTATTGGTCCTGGTGGT
ACAGCCAGTGCCAACTTCGCTGCCAACTTTGGTGCCATTGGTTTCTTCTGGGTTGAGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001005332
Insert Size 2337 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001005332.1
RefSeq Size 2665 bp
RefSeq ORF 2337 bp
Locus ID 9500
UniProt ID Q9Y5V3
Protein Families Druggable Genome
Protein Pathways Neurotrophin signaling pathway
MW 86.2 kDa
Gene Summary This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode isoform b.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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