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SREBP1 (SREBF1) (NM_001005291) Human Untagged Clone
CAT＃: SC300896
SREBF1 (untagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 1
CNY 12,304.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHd1; HMD; IFAP2; SREBP1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC300896 sequence for NM_001005291 edited (data generated by NextGen Sequencing)
ATGGACGAGCCACCCTTCAGCGAGGCGGCTTTGGAGCAGGCGCTGGGCGAGCCGTGCGATCTGGACGCGG CGCTGCTGACCGACATCGAAGGTGAAGTCGGCGCGGGGAGGGGTAGGGCCAACGGCCTGGACGCCCCAAG GGCGGGCGCAGATCGCGGAGCCATGGATTGCACTTTCGAAGACATGCTTCAGCTTATCAACAACCAAGAC AGTGACTTCCCTGGCCTATTTGACCCACCCTATGCTGGGAGTGGGGCAGGGGGCACAGACCCTGCCAGCC CCGATACCAGCTCCCCAGGCAGCTTGTCTCCACCTCCTGCCACATTGAGCTCCTCTCTTGAAGCCTTCCT GAGCGGGCCGCAGGCAGCGCCCTCACCCCTGTCCCCTCCCCAGCCTGCACCCACTCCATTGAAGATGTAC CCGTCCATGCCCGCTTTCTCCCCTGGGCCTGGTATCAAGGAAGAGTCAGTGCCACTGAGCATCCTGCAGA CCCCCACCCCACAGCCCCTGCCAGGGGCCCTCCTGCCACAGAGCTTCCCAGCCCCAGCCCCACCGCAGTT CAGCTCCACCCCTGTGTTAGGCTACCCCAGCCCTCCGGGAGGCTTCTCTACAGGAAGCCCTCCCGGGAAC ACCCAGCAGCCGCTGCCTGGCCTGCCACTGGCTTCCCCGCCAGGGGTCCCGCCCGTCTCCTTGCACACCC AGGTCCAGAGTGTGGTCCCCCAGCAGCTACTGACAGTCACAGCTGCCCCCACGGCAGCCCCTGTAACGAC CACTGTGACCTCGCAGATCCAGCAGGTCCCGGTCCTGCTGCAGCCCCACTTCATCAAGGCAGACTCGCTG CTTCTGACAGCCATGAAGACAGACGGAGCCACTGTGAAGGCGGCAGGTCTCAGTCCCCTGGTCTCTGGCA CCACTGTGCAGACAGGGCCTTTGCCGACCCTGGTGAGTGGCGGAACCATCTTGGCAACAGTCCCACTGGT CGTAGATGCGGAGAAGCTGCCTATCAGCCGGCTCGCAGCTGGCAGCAAGGCCCCGGCCTCTGCCCAGAGC CGTGGAGAGAAGCGCACAGCCCACAACGCCATTGAGAAGCGCTACCGCTCCTCCATCAATGACAAAATCA TTGAGCTCAAGGATCTGGTGGTGGGCACTGAGGCAAAGCTGAATAAATCTGCTGTCTTGCGCAAGGCCAT CGACTACATTCGCTTTCTGCAACACAGCAACCAGAAACTCAAGCAGGAGAACCTAAGTCTGCGCACTGCT GTCCACAAAAGCAAATCTCTGAAGGATCTGGTGTCGGCCTGTGGCAGTGGAGGGAACACAGACGTGCTCA TGGAGGGCGTGAAGACTGAGGTGGAGGACACACTGACCCCACCCCCCTCGGATGCTGGCTCACCTTTCCA GAGCAGCCCCTTGTCCCTTGGCAGCAGGGGCAGTGGCAGCGGTGGCAGTGGCAGTGACTCGGAGCCTGAC AGCCCAGTCTTTGAGGACAGCAAGGCAAAGCCAGAGCAGCGGCCGTCTCTGCACAGCCGGGGCATGCTGG ACCGCTCCCGCCTGGCCCTGTGCACGCTCGTCTTCCTCTGCCTGTCCTGCAACCCCTTGGCCTCCTTGCT GGGGGCCCGGGGGCTTCCCAGCCCCTCAGATACCACCAGCGTCTACCATAGCCCTGGGCGCAACGTGCTG GGCACCGAGAGCAGAGATGGCCCTGGCTGGGCCCAGTGGCTGCTGCCCCCAGTGGTCTGGCTGCTCAATG GGCTGTTGGTGCTCGTCTCCTTGGTGCTTCTCTTTGTCTACGGTGAGCCAGTCACACGGCCCCACTCAGG CCCCGCCGTGTACTTCTGGAGGCATCGCAAGCAGGCTGACCTGGACCTGGCCCGGGGAGACTTTGCCCAG GCTGCCCAGCAGCTGTGGCTGGCCCTGCGGGCACTGGGCCGGCCCCTGCCCACCTCCCACCTGGACCTGG CTTGTAGCCTCCTCTGGAACCTCATCCGTCACCTGCTGCAGCGTCTCTGGGTGGGCCGCTGGCTGGCAGG CCGGGCAGGGGGCCTGCAGCAGGACTGTGCTCTGCGAGTGGATGCTAGCGCCAGCGCCCGAGACGCAGCC CTGGTCTACCATAAGCTGCACCAGCTGCACACCATGGGGAAGCACACAGGCGGGCACCTCACTGCCACCA ACCTGGCGCTGAGTGCCCTGAACCTGGCAGAGTGTGCAGGGGATGCCGTGTCTGTGGCGACGCTGGCCGA GATCTATGTGGCGGCTGCATTGAGAGTGAAGACCAGTCTCCCACGGGCCTTGCATTTTCTGACACGCTTC TTCCTGAGCAGTGCCCGCCAGGCCTGCCTGGCACAGAGTGGCTCAGTGCCTCCTGCCATGCAGTGGCTCT GCCACCCCGTGGGCCACCGTTTCTTCGTGGATGGGGACTGGTCCGTGCTCAGTACCCCATGGGAGAGCCT GTACAGCTTGGCCGGGAACCCAGTGGACCCCCTGGCCCAGGTGACTCAGCTATTCCGGGAACATCTCTTA GAGCGAGCACTGAACTGTGTGACCCAGCCCAACCCCAGCCCTGGGTCAGCTGATGGGGACAAGGAATTCT TGGATGCCCTCGGGTACCTGCAGCTGCTGAACAGCTGTTCTGATGCTGCGGGGGCTCCTGCCTACAGCTT CTCCATCAGTTCCAGCATGGCCACCACCACCGGCGTAGACCCGGTGGCCAAGTGGTGGGCCTCTCTGACA GCTGTGGTGATCCACTGGCTGCGGCGGGATGAGGAGGCGGCTGAGCGGCTGTGCCCGCTGGTGGAGCACC TGCCCCGGGTGCTGCAGGAGTCTGAGAGACCCCTGCCCAGGGCAGCTCTGCACTCCTTCAAGGCTGCCCG GGCCCTGCTGGGCTGTGCCAAGGCAGAGTCTGGTCCAGCCAGCCTGACCATCTGTGAGAAGGCCAGTGGG TACCTGCAGGACAGCCTGGCTACCACACCAGCCAGCAGCTCCATTGACAAGGCCGTGCAGCTGTTCCTGT GTGACCTGCTTCTTGTGGTGCGCACCAGCCTGTGGCGGCAGCAGCAGCCCCCGGCCCCGGCCCCAGCAGC CCAGGGCACCAGCAGCAGGCCCCAGGCTTCCGCCCTTGAGCTGCGTGGCTTCCAACGGGACCTGAGCAGC CTGAGGCGGCTGGCACAGAGCTTCCGGCCCGCCATGCGGAGGGTGTTCCTACATGAGGCCACGGCCCGGC TGATGGCGGGGGCCAGCCCCACACGGACACACCAGCTCCTCGACCGCAGTCTGAGGCGGCGGGCAGGCCC CGGTGGCAAAGGAGGCGCGGTGGCGGAGCTGGAGCCGCGGCCCACGCGGCGGGAGCACGCGGAGGCCTTG CTGCTGGCCTCCTGCTACCTGCCCCCCGGCTTCCTGTCGGCGCCCGGGCAGCGCGTGGGCATGCTGGCTG AGGCGGCGCGCACACTCGAGAAGCTTGGCGATCGCCGGCTGCTGCACGACTGTCAGCAGATGCTCATGCG CCTGGGCGGTGGGACCACTGTCACTTCCAGCTAG Clone variation with respect to NM_001005291.2 1007:g=>a 2591:t=>c |
| Restriction Sites | Please inquire |
| ACCN | NM_001005291 |
| Insert Size | 4300 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to contain 2 SNPs compared with NM_001005291.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001005291.1, NP_001005291.1 |
| RefSeq Size | 5012 bp |
| RefSeq ORF | 3534 bp |
| Locus ID | 6720 |
| UniProt ID | P36956 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Insulin signaling pathway |
| Gene Summary | This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209183 | SREBF1 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 1 |
CNY 12,288.00 |
|
| RC209183L1 | Lenti-ORF clone of SREBF1 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 1 |
CNY 14,688.00 |
|
| RC209183L2 | Lenti-ORF clone of SREBF1 (mGFP-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 1 |
CNY 13,680.00 |
|
| RC209183L3 | Lenti-ORF clone of SREBF1 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 1 |
CNY 13,680.00 |
|
| RC209183L4 | Lenti-ORF clone of SREBF1 (mGFP-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 1 |
CNY 13,680.00 |
|
| RG209183 | SREBF1 (tGFP-tagged) - Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 1 |
CNY 13,888.00 |
