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PDE1A (NM_001003683) Human Untagged Clone
CAT＃: SC300512
PDE1A (untagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 2
CN¥ 8,550.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAM-PDE-1A; CAM-PDE 1A; HCAM-1; HCAM1; HSPDE1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC300512 representing NM_001003683.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGTCTAGTGCCACAGAGATTGAAGAATTGGAAAACACCACTTTTAAGTATCTTACAGGAGAACAG ACTGAAAAAATGTGGCAGCGCCTGAAAGGAATACTAAGATGCTTGGTGAAGCAGCTGGAAAGAGGTGAT GTTAACGTCGTCGACTTAAAGAAGAATATTGAATATGCGGCATCTGTGCTGGAAGCAGTTTATATCGAT GAAACAAGAAGACTTCTGGATACTGAAGATGAGCTCAGTGACATTCAGACTGACTCAGTCCCATCTGAA GTCCGGGACTGGTTGGCTTCTACCTTTACACGGAAAATGGGGATGACAAAAAAGAAACCTGAGGAAAAA CCAAAATTTCGGAGCATTGTGCATGCTGTTCAAGCTGGAATTTTTGTGGAAAGAATGTACCGAAAAACA TATCATATGGTTGGTTTGGCATATCCAGCAGCTGTCATCGTAACATTAAAGGATGTTGATAAATGGTCT TTCGATGTATTTGCCCTAAATGAAGCAAGTGGAGAGCATAGTCTGAAGTTTATGATTTATGAACTGTTT ACCAGATATGATCTTATCAACCGTTTCAAGATTCCTGTTTCTTGCCTAATCACCTTTGCAGAAGCTTTA GAAGTTGGTTACAGCAAGTACAAAAATCCATATCACAATTTGATTCATGCAGCTGATGTCACTCAAACT GTGCATTACATAATGCTTCATACAGGTATCATGCACTGGCTCACTGAACTGGAAATTTTAGCAATGGTC TTTGCTGCTGCCATTCATGATTATGAGCATACAGGGACAACAAACAACTTTCACATTCAGACAAGGTCA GATGTTGCCATTTTGTATAATGATCGCTCTGTCCTTGAGAATCACCACGTGAGTGCAGCTTATCGACTT ATGCAAGAAGAAGAAATGAATATCTTGATAAATTTATCCAAAGATGACTGGAGGGATCTTCGGAACCTA GTGATTGAAATGGTTTTATCTACAGACATGTCAGGTCACTTCCAGCAAATTAAAAATATAAGAAACAGT TTGCAGCAGCCTGAAGGGATTGACAGAGCCAAAACCATGTCCCTGATTCTCCACGCAGCAGACATCAGC CACCCAGCCAAATCCTGGAAGCTGCATTATCGGTGGACCATGGCCCTAATGGAGGAGTTTTTCCTGCAG GGAGATAAAGAAGCTGAATTAGGGCTTCCATTTTCCCCACTTTGTGATCGGAAGTCAACCATGGTGGCC CAGTCACAAATAGGTTTCATCGATTTCATAGTAGAGCCAACATTTTCTCTTCTGACAGACTCAACAGAG AAAATTGTTATTCCTCTTATAGAGGAAGCCTCAAAAGCCGAAACTTCTTCCTATGTGGCAAGCAGCTCA ACCACCATTGTGGGGTTACACATTGCTGATGCACTAAGACGATCAAATACAAAAGGCTCCATGAGTGAT GGGTCCTATTCCCCAGACTACTCCCTTGCAGCAGTGGACCTGAAGAGTTTCAAGAACAACCTGGTGGAC ATCATTCAGCAGAACAAAGAGAGGTGGAAAGAGTTAGCTGCACAAGAAGCAAGAACCAGTTCACAGAAG TGTGAGTTTATTCATCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001003683 |
| Insert Size | 1608 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001003683.2 |
| RefSeq Size | 2159 bp |
| RefSeq ORF | 1608 bp |
| Locus ID | 5136 |
| UniProt ID | P54750 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction |
| MW | 61.3 kDa |
| Gene Summary | Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009] Transcript Variant: This variant (2) differs in the 5' and 3' UTRs, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223864 | PDE1A (Myc-DDK-tagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 2 |
CN¥ 5,976.00 |
|
| RC223864L1 | Lenti-ORF clone of PDE1A (Myc-DDK-tagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 2 |
CN¥ 8,376.00 |
|
| RC223864L2 | Lenti-ORF clone of PDE1A (mGFP-tagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 2 |
CN¥ 6,460.00 |
|
| RC223864L3 | Lenti-ORF clone of PDE1A (Myc-DDK-tagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 2 |
CN¥ 6,460.00 |
|
| RC223864L4 | Lenti-ORF clone of PDE1A (mGFP-tagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 2 |
CN¥ 6,460.00 |
|
| RG223864 | PDE1A (tGFP-tagged) - Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 2 |
CN¥ 5,040.00 |
