PI4KA (NM_002650) Human Untagged Clone
CAT#: SC128296
PI4KA (untagged)-Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 1
CNY 6,264.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | PI4K-ALPHA; pi4K230; PIK4CA |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF sequence for NM_002650 edited
ATGCGGGAGATGGCAGGGGCCTGGCACATGACGGTGGAGCAGAAATTTGGCCTGTTTTCT GCTGAGATAAAGGAAGCAGACCCCCTGGCTGCCTCGGAAGCAAGTCAACCCAAACCCTGT CCCCCCGAAGTGACCCCCCACTACATCTGGATCGACTTCCTGGTGCAGCGGTTTGAGATC GCCAAGTACTGCAGCTCTGACCAAGTGGAGATCTTCTCCAGCCTGCTGCAGCGCTCCATG TCCCTGAACATCGGCGGGGCCAAGGGGAGCATGAACCGGCACGTGGCGGCCATCGGGCCC CGCTTCAAGCTGCTGACCCTGGGGCTGTCCCTCCTGCATGCCGATGTGGTTCCAAATGCA ACCATCCGCAATGTGCTTCGCGAGAAGATCTACTCCACTGCCTTTGACTACTTCAGCTGT CCCCCAAAGTTCCCTACTCAAGGAGAGAAGCGGCTGCGTGAAGACATAAGCATCATGATT AAATTTTGGACCGCCATGTTCTCAGATAAGAAGTACCTGACCGCCAGCCAGCTTGTTCCC CCAGATAATCAGGACACCCGGAGCAACCTGGACATAACTGTCGGCTCTCGGCAACAAGCC ACCCAAGGCTGGATCAACACATACCCCCTGTCCAGCGGCATGTCCACCATCTCCAAGAAA TCAGGCATGTCTAAGAAAACCAACCGGGGCTCCCAGCTGCACAAATACTACATGAAGCGC AGGACGCTGCTGCTGTCCCTGCTGGCCACTGAGATCGAGCGTCTCATCACATGGTACAAC CCGCTGTCAGCCCCGGAACTGGAACTAGACCAGGCCGGAGAGAACAGCGTGGCCAACTGG AGATCTAAGTACATCAGCCTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTCGCCTGG AGCATCTCTCCCTACCTAGCCGTGCAGCTGCCTGCCAGGTTTAAGAACACAGAAGCCATT GGGAACGAAGTGACCCGTCTCGTTCGGTTGGACCCGGGAGCCGTTAGTGATGTGCCTGAA GCAATCAAGTTCCTGGTCACCTGGCACACCATCGACGCCGATGCTCCAGAGCTCAGCCAT GTGCTGTGCTGGGCGCCCACGGACCCACCCACAGGCCTCTCCTACTTCTCCAGCATGTAC CCGCCGCACCCTCTCACGGCGCAGTACGGGGTGAAAGTCCTGCGGTCCTTCCCTCCGGAC GCCATCCTCTTCTACATCCCCCAGATTGTGCAGGCCCTCAGGTACGACAAGATGGGCTAT GTGCGGGAGTATATTCTGTGGGCAGCGTCTAAATCCCAGCTTCTGGCACACCAGTTCATC TGGAACATGAAGACTAACATTTATCTAGATGAAGAGGGCCACCAGAAAGACCCTGACATC GGCGACCTCCTGGATCAGTTGGTAGAGGAGATCACAGGCTCCTTGTCCGGCCCAGCGAAG GACTTTTACCAGCGGGAGTTTGATTTCTTTAACAAGATCACCAACGTGTCGGCTATCATC AAGCCCTACCCTAAAGGCGACGAGAGAAAGAAGGCTTGTCTGTCGGCCCTGTCTGAAGTG AAGGTGCAGCCGGGCTGCTACCTGCCCAGCAACCCTGAGGCCATTGTGCTGGACATCGAC TACAAGTCTGGGACCCCGATGCAGAGTGCTGCAAAAGCCCCATATCTGGCCAAGTTCAAG GTGAAGCGATGTGGAGTTAGTGAACTTGAAAAAGAAGGTCTGCGGTGCCGCTCAGACTCC GAGGATGAGTGCAGCACGCAGGAGGCCGACGGCCAGAAGATCTCCTGGCAGGCAGCCATC TTCAAGGTGGGAGACGACTGCCGGCAGGACATGCTGGCCCTGCAGATCATCGACCTCTTC AAGAACATCTTCCAGCTGGTCGGCCTGGACCTCTTTGTTTTTCCCTACCGCGTGGTGGCC ACTGCCCCTGGGTGCGGGGTGATCGAGTGCATCCCCGACTGCACCTCCCGGGACCAGCTG GGCCGCCAGACAGACTTCGGCATGTACGACTACTTCACACGCCAGTACGGGGATGAGTCC ACTCTGGCCTTCCAGCAGGCCCGCTACAACTTCATCCGAAGCATGGCCGCCTACAGCCTC CTGCTGTTCCTGCTGCAGATCAAGGACAGACACAACGGCAACATTATGCTGGACAAGAAG GGTCATATCATCCACATCGACTTTGGCTTCATGTTTGAAAGCTCGCCGGGCGGCAATCTC GGCTGGGAACCCGACATCAAGCTGACGGATGAGATGGTGATGATCATGGGGGGCAAGATG GAGGCCACACCCTTCAAGTGGTTCATGGAGATGTGTGTCCGAGGCTACCTGGCTGTGCGG CCCTACATGGACGCGGTCGTCTCCCTGGTCACTCTCATGTTGGACACGGGCCTGCCCTGT TTTCGCGGCCAGACAATCAAGCTCTTGAAGCACAGGTTTAGCCCCAACATGACTGAGCGC GAGGCTGCAAATTTCATCATGAAGGTCATCCAGAGCTGCTTCCTCAGCAACAGGAGCCGG ACCTACGACATGATCCAGTACTATCAGAATGACATCCCCTACTGA >OriGene 5' read for NM_002650 unedited
TACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAGTGGCTGCTGGCTGGCAAGN ATGGAGTGGAAGTGCCGTTCATGCGGGAGATGGCAGGGGCCTGGCACATGACGGTGGAGC AGAAATTTGGCCTGTTTTCTGCTGAGATAAAGGAAGCAGACCCCCTGGCTGCCTCGGAAG CAAGTCAACCCAAACCCTGTCCCCCCGAAGTGACCCCCCACTACATCTGGATCGACTTCC TGGTGCAGCGGTTTGAGATCGCCAAGTACTGCAGCTCTGACCAAGTGGAGATCTTCTCCA GCCTGCTGCAGCGCTCCATGTCCCTGAACATCGGCGGGGCCAAGGGGAGCATGAACCGGC ACGTGGCGGCCATCGGGCCCCGCTTCAAGCTGCTGACCCTGGGGCTGTCCCTCCTGCATG CCGATGTGGTTCCAAATGCAACCATCCGCAATGTGCTTCGCGAGAAGATCTACTCCACTG CCTTTGACTACTTCAGCTGTCCCCCAAAGTTCCCTACTCAAGGAGAGAAGCGGCTGCGTG AAGACATAAGCATCATGATTAAATTTTGGACCGCCATGTTCTCAATTAAGAAGTACCTGA CCGCCAGCCAGCTTGTTCCCCCAGATAATCAGGACACCCGGAGCAACCTGACCATAACTG TCGGCTCTCGCAACAAGCCACCCAAGCCTGGATCACACATACCCCCTGTCCAGCGGCATG TCCACCATCTCCAGAAATCCAGCATGTCTAAAAAACCAACCGGGGGCTCCAGCTGCCANA TACTACATGAAGCGCAGGAGCTTNTTGCTGCCCTGCTGCCACTGAAAATCGACGTTAATA CTGGTCAAACCCCTGTAGCCCCGGACTGGAATAAACAGCCGGAAAAAAAGGTGGCACCTG GAAAATAAGACATACCCTGGGAAAGCAGGGGAAGCCACGGGACCTTCTGGAA >OriGene 3' read for NM_002650 unedited
CGTCGGATGCACTTCAGGGCCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGG GATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTT TTTTTTTTCACTATTTTGGGTTTTTATTTTGTTGATGTTGGTTAAATCTTATCTCTTTTT TTATACACAATACTTCATGTACCTATGAAATAAGACAGGTAGGGAATATGTCCAGTGCAA ACAGAGGACTCACACCTGTGCATAGACAGCACCATCCATTGATTGTCGCTGCAGTCCATG GCGTTACCAAGGCTGCGCCACCCACGTGCTGCCCCAGGAGGCGCTACCAGGTTCTTTGGG CCACAGGCCTCTCCTCCACTGCATGTGGCGGCAGGGCAGGGAGGTCGCAGGGCTCCATGA TTGTGGGACAGCTTTGAGGGCACATGGGGCAAAGGCCCTCGAAGGTCCCCTCCTCAGTAG GGGATGTCATTCTGATAGTACTGGATCATGTCGTAGGTCCGGCTCCTGTTGCTGAGGAAG CAGCTCTGGATGACCTTCATGATGAAATTTGCAGCCTCGCGCTCAGTCATGTTGGGGCTA AACCTGTGCTTCAAGAGCTTGATTGTCTGGCCGCGAAAACAGGGCAGGCCCGTGTCCAAC ATGAGAGTGACCAGGGAGACGACCGCGTCCATGTAGGGCCGCACAGCCAGGTAGCCTCGG ACACACATCTCCATGAACCACTTGAAGGTGTGGCCTCCATCTTGCCCCCCATGATCATCA CCATCTCATCCGTCAGCTTGATGTCGGGTTCCCAGCCCGAGATTGCCGCCCGGCGAGCTT TCAACATGAGCAAGTCGATGTGATGATTGACCTCTGTCAGCATATGTGCGTTGTGTCTGT CTGATTGCACAGGACGCAGAGCCTGTAGCGCATGCCTCGGATGATTGAACGGCTGCTGAG CGAGTGGACTA |
Restriction Sites | NotI-NotI |
ACCN | NM_002650 |
Insert Size | 3000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002650.1, NP_002641.1 |
RefSeq Size | 3034 bp |
RefSeq ORF | 2565 bp |
Locus ID | 5297 |
Domains | PI3_PI4_kinase, PI3Ka |
Protein Families | Druggable Genome |
Protein Pathways | Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
Gene Summary | This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2018] Transcript Variant: This variant (2) differs in the 5' UTR and CDS compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218825 | PI4KA (Myc-DDK-tagged)-Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 1 |
CNY 6,256.00 |
|
RC218825L1 | Lenti ORF clone of Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 1, Myc-DDK-tagged |
CNY 10,450.00 |
|
RC218825L2 | Lenti ORF clone of Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 1, mGFP tagged |
CNY 10,450.00 |
|
RC218825L3 | Lenti ORF clone of Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 1, Myc-DDK-tagged |
CNY 8,656.00 |
|
RC218825L4 | Lenti ORF clone of Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 1, mGFP tagged |
CNY 10,450.00 |
|
RG218825 | PI4KA (tGFP-tagged) - Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 1 |
CNY 7,856.00 |