TCIRG1 (NM_006019) Human Untagged Clone
CAT#: SC128192
TCIRG1 (untagged)-Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1
CNY 7,220.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | a3; Atp6i; ATP6N1C; ATP6V0A3; OC-116kDa; OC116; OPTB1; Stv1; TIRC7; Vph1 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_006019, the custom clone sequence may differ by one or more nucleotides
ATGGGCTCCATGTTCCGGAGCGAGGAGGTGGCCCTGGTCCAGCTCTTTCTGCCCACAGCGGCTGCCTACA CCTGCGTGAGTCGGCTGGGCGAGCTGGGCCTCGTGGAGTTCAGAGACCTCAACGCCTCGGTGAGCGCCTT CCAGAGACGCTTTGTGGTTGATGTTCGGCGCTGTGAGGAGCTGGAGAAGACCTTCACCTTCCTGCAGGAG GAGGTGCGGCGGGCTGGGCTGGTCCTGCCCCCGCCAAAGGGGAGGCTGCCGGCACCCCCACCCCGGGACC TGCTGCGCATCCAGGAGGAGACGGAGCGCCTGGCCCAGGAGCTGCGGGATGTGCGGGGCAACCAGCAGGC CCTGCGGGCCCAGCTGCACCAGCTGCAGCTCCACGCCGCCGTGCTACGCCAGGGCCATGAACCTCAGCTG GCAGCCGCCCACACAGATGGGGCCTCAGAGAGGACGCCCCTGCTCCAGGCCCCCGGGGGGCCGCACCAGG ACCTGAGGGTCAACTTTGTGGCAGGTGCCGTGGAGCCCCACAAGGCCCCTGCCCTAGAGCGCCTGCTCTG GAGGGCCTGCCGCGGCTTCCTCATTGCCAGCTTCAGGGAGCTGGAGCAGCCGCTGGAGCACCCCGTGACG GGCGAGCCAGCCACGTGGATGACCTTCCTCATCTCCTACTGGGGTGAGCAGATCGGACAGAAGATCCGCA AGATCACGGACTGCTTCCACTGCCACGTCTTCCCGTTTCTGCAGCAGGAGGAGGCCCGCCTCGGGGCCCT GCAGCAGCTGCAACAGCAGAGCCAGGAGCTGCAGGAGGTCCTCGGGGAGACAGAGCGGTTCCTGAGCCAG GTGCTAGGCCGGGTGCTGCAGCTGCTGCCGCCAGGGCAGGTGCAGGTCCACAAGATGAAGGCCGTGTACC TGGCCCTGAACCAGTGCAGCGTGAGCACCACGCACAAGTGCCTCATTGCCGAGGCCTGGTGCTCTGTGCG AGACCTGCCCGCCCTGCAGGAGGCCCTGCGGGACAGCTCGATGGAGGAGGGAGTGAGTGCCGTGGCTCAC CGCATCCCCTGCCGGGACATGCCCCCCACACTCATCCGCACCAACCGCTTCACGGCCAGCTTCCAGGGCA TCGTGGATGCCTACGGCGTGGGCCGCTACCAGGAGGTCAACCCCGCTCCCTACACCATCATCACCTTCCC CTTCCTGTTTGCTGTGATGTTCGGGGATGTGGGCCACGGGCTGCTCATGTTCCTCTTCGCCCTGGCCATG GTCCTTGCGGAGAACCGACCGGCTGTGAAGGCCGCGCAGAACGAGATCTGGCAGACTTTCTTCAGGGGCC GCTACCTGCTCCTGCTTATGGGCCTGTTCTCCATCTACACCGGCTTCATCTACAACGAGTGCTTCAGTCG CGCCACCAGCATCTTCCCCTCGGGCTGGAGTGTGGCCGCCATGGCCAACCAGTCTGGCTGGAGTGATGCA TTCCTGGCCCAGCACACGATGCTTACCCTGGATCCCAACGTCACCGGTGTCTTCCTGGGACCCTACCCCT TTGGCATCGATCCTATTTGGAGCCTGGCTGCCAACCACTTGAGCTTCCTCAACTCCTTCAAGATGAAGAT GTCCGTCATCCTGGGCGTCGTGCACATGGCCTTTGGGGTGGTCCTCGGAGTCTTCAACCACGTGCACTTT GGCCAGAGGCACCGGCTGCTGCTGGAGACGCTGCCGGAGCTCACCTTCCTGCTGGGACTCTTCGGTTACC TCGTGTTCCTAGTCATCTACAAGTGGCTGTGTGTCTGGGCTGCCAGGGCCGCCTCGGCCCCCAGCATCCT CATCCACTTCATCAACATGTTCCTCTTCTCCCACAGCCCCAGCAACAGGCTGCTCTACCCCCGGCAGGAG GTGGTCCAGGCCACGCTGGTGGTCCTGGCCTTGGCCATGGTGCCCATCCTGCTGCTTGGCACACCCCTGC ACCTGCTGCACCGCCACCGCCGCCGCCTGCGGAGGAGGCCCGCTGACCGACAGGAGGAAAACAAGGCCGG GTTGCTGGACCTGCCTGACGCATCTGTGAATGGCTGGAGCTCCGATGAGGAAAAGGCAGGGGGCCTGGAT GATGAAGAGGAGGCCGAGCTCGTCCCCTCCGAGGTGCTCATGCACCAGGCCATCCACACCATCGAGTTCT GCCTGGGCTGCGTCTCCAACACCGCCTCCTACCTGCGCCTGTGGGCCCTGAGCCTGGCCCACGCCCAGCT GTCCGAGGTTCTGTGGGCCATGGTGATGCGCATAGGCCTGGGCCTGGGCCGGGAGGTGGGCGTGGCGGCT GTGGTGCTGGTCCCCATCTTTGCCGCCTTTGCCGTGATGACCGTGGCTATCCTGCTGGTGATGGAGGGAC TCTCAGCCTTCCTGCACGCCCTGCGGCTGCACTGGGTGGAATTCCAGAACAAGTTCTACTCAGGCACGGG CTACAAGCTGAGTCCCTTCACCTTCGCTGCCACAGATGACTAG >OriGene 5' read for NM_006019 unedited
GAATTTGTAATACGACTCACTCATAGGGCGGCCGCGAATTCGCACGAGGCGGAGCGGGGC AGCCAGCAGCGGAGGCGCGGCGCGCAGCACACCCGGGGACCATGGGCTCCATGTTCCGGA GCGAGGAGGTGGCCCTGGTCCAGCTCTTTCTGCCCACAGCGGCTGCCTACACCTGCGTGA GTCGGCTGGGCGAGCTGGGCCTCGTGGAGTTCAGAGACCTCAACGCCTCGGTGAGCGCCT TCCAGAGACGCTTTGTGGTTGATGTTCGGCGCTGTGAGGAGCTGGAGAAGACCTTCACCT TCCTGCAGGAGGAGGTGCGGCGGGCTGGGCTGGTCCTGCCCCCGCCAAAGGGGAGGCTGC CGGCACCCCCACCCCGGGACCTGCTGCGCATCCAGGAGGAGACGGAGCGCCTGGCCCAGG AGCTGCGGGATGTGCGGGGCAACCAGCAGGCCCTGCGGGCCCAGCTGCACCAGCTGCAGC TCCACGCCGCCGTGCTACGCCAGGGCCATGAACCTCAGCTGGCAGCCGCCCACACAGATG GGGCCTCAGAGAGGACGCCCCTGCTCCAGGCCCCCGGGGGGCCGCACCANGACCTGAGGG TCAACTNTGTGGCATGTGCCGTGGAGCCCCACATGCCCCTGCCCTATAGCGCCTGCTCTG GATGGCCTGCCGCGGCTTCCTCATTGCCAGCTTCAGGGAGCTGGAGCAGCCGCTGGAGCA CCCCGTGACGATCGGACAGAAGATCCCGCAGATCACGGACTGCTTCCACTGCCACGTCTT NCCGTTTCTGCAGCAGGAAGATGCCCGCCTCGGGGCCTGCAGCAGCTGCAACAGCAAAGC CAGAGCTGGCANGAGGTCCCTCGGGGAGACAGAGCGGNTCCTGATCCCAGTGCTATGCCN GGNTGCTGCATCTGCTGCCGCAGGGNCAGTGCANGTCCACAAATGAAAGGCGTGTACCTG CCCCTGACATTGCATGTGAGCACAGCACATTGCCTCATGCGAGCCTGNGCTCTGGCAGAC TGCCCGCTGAGAGCN >OriGene 3' read for NM_006019 unedited
NNNNCCCAACTGTGAACCGCGGCTCGCAATCTAGGATCAGTTTTGTTTTATGGTTTATAT TTTTGCCTTTTTTTGTTTTGTTTAGGGCAAAGGCGTACATCTTTATTCCTCTCCTGACTC AAGAGTCAAGAAAGAGGTCTGGCAGGACCTGCAGTGGGCCCTAATCAACTGTGGCACCGA AAGTGAAAGGACTCAACTTGTGACCCGTGCCTGAATAAAACTTGGTCTGGAATTCCACCC CTTGCAACCCCAGGGCGTGCAAGAAGGCTGAAAATCCCTCCATCACCAGCCGGATAACCA CGGGCATTACGGCTAAAGCCGCAAAAATGGGGACCAGTACCACAAGCGCCAAGCCCACCT CCCGGCCCAGGGCCAAGCCTATGCGCATCACCATGGCCCACAAAACCTCGGACAACTGGG CGTGGGCCAAGCTCAAGGCCCACAAGCACAAGNCAAGACGCTGTGTTGGAGACTCAGCCC AACAAAACTTCAAGGTGTGGATTGCCTGTGCATGAGCACCTCGGGAGGGGAGACAAGCTG GGGCTTCTTGTTATTATCCAAGCCCCCTGGTNNNTCTTTCCTATGGAGCCCCGGGCCTTT TCCAAAATGGTAAGGAAGGGTCCCGCACCCCGGCCCTGTTTTCCCCCCCGCGGGCAAAGG GCCCCCCTCCCAAAAAGGGGTTTAATGGAGGTTCCACACAGTTCCAGGTTTTCCCCCAAT TCTTGTTGGGGCCCCATGGCAAAGGCCCGAACCCCACAGGGGGGCCGGGGCCCACCCCCC CCCGGGGGGAGAAAAAACCCTTTTTTCGGGGGTTTGTGGCAAAAAAAAAAAACCTTTTAA AAAAAGGGGTAAGAAATCCCCGGGGCCCCCAGGGGGGCCTTTTAACCCCCATAAAACAGA GCCCTTTTTG |
Restriction Sites | NotI-NotI |
ACCN | NM_006019 |
Insert Size | 2700 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006019.2, NP_006010.2 |
RefSeq Size | 2700 bp |
RefSeq ORF | 2493 bp |
Locus ID | 10312 |
UniProt ID | Q13488 |
Domains | V_ATPase_sub_a |
Protein Families | Transmembrane |
Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
Gene Summary | This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, May 2017] Transcript Variant: This variant (1) encodes isoform a, also known as the OC116 protein because it is one of three V-ATPase 116 kD subunits. It is also referred to as the a3 isoform and is induced on osteoclast differentiation. This variant contains a unique 5' end and lacks an internal segment compared to variant 2, which results in an isoform (a) with a different N-terminus than that of isoform b. |
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Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202125 | TCIRG1 (Myc-DDK-tagged)-Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1 |
CNY 6,080.00 |
|
RC202125L1 | Lenti ORF clone of Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1, Myc-DDK-tagged |
CNY 8,480.00 |
|
RC202125L2 | Lenti ORF clone of Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
RC202125L3 | Lenti ORF clone of Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
RC202125L4 | Lenti ORF clone of Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
RG202125 | TCIRG1 (tGFP-tagged) - Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1 |
CNY 7,680.00 |