TCIRG1 (NM_006019) Human Untagged Clone

CAT#: SC128192

TCIRG1 (untagged)-Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1



  "NM_006019" in other vectors (6)

CNY 7,220.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
TCIRG1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms a3; Atp6i; ATP6N1C; ATP6V0A3; OC-116kDa; OC116; OPTB1; Stv1; TIRC7; Vph1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_006019, the custom clone sequence may differ by one or more nucleotides


ATGGGCTCCATGTTCCGGAGCGAGGAGGTGGCCCTGGTCCAGCTCTTTCTGCCCACAGCGGCTGCCTACA
CCTGCGTGAGTCGGCTGGGCGAGCTGGGCCTCGTGGAGTTCAGAGACCTCAACGCCTCGGTGAGCGCCTT
CCAGAGACGCTTTGTGGTTGATGTTCGGCGCTGTGAGGAGCTGGAGAAGACCTTCACCTTCCTGCAGGAG
GAGGTGCGGCGGGCTGGGCTGGTCCTGCCCCCGCCAAAGGGGAGGCTGCCGGCACCCCCACCCCGGGACC
TGCTGCGCATCCAGGAGGAGACGGAGCGCCTGGCCCAGGAGCTGCGGGATGTGCGGGGCAACCAGCAGGC
CCTGCGGGCCCAGCTGCACCAGCTGCAGCTCCACGCCGCCGTGCTACGCCAGGGCCATGAACCTCAGCTG
GCAGCCGCCCACACAGATGGGGCCTCAGAGAGGACGCCCCTGCTCCAGGCCCCCGGGGGGCCGCACCAGG
ACCTGAGGGTCAACTTTGTGGCAGGTGCCGTGGAGCCCCACAAGGCCCCTGCCCTAGAGCGCCTGCTCTG
GAGGGCCTGCCGCGGCTTCCTCATTGCCAGCTTCAGGGAGCTGGAGCAGCCGCTGGAGCACCCCGTGACG
GGCGAGCCAGCCACGTGGATGACCTTCCTCATCTCCTACTGGGGTGAGCAGATCGGACAGAAGATCCGCA
AGATCACGGACTGCTTCCACTGCCACGTCTTCCCGTTTCTGCAGCAGGAGGAGGCCCGCCTCGGGGCCCT
GCAGCAGCTGCAACAGCAGAGCCAGGAGCTGCAGGAGGTCCTCGGGGAGACAGAGCGGTTCCTGAGCCAG
GTGCTAGGCCGGGTGCTGCAGCTGCTGCCGCCAGGGCAGGTGCAGGTCCACAAGATGAAGGCCGTGTACC
TGGCCCTGAACCAGTGCAGCGTGAGCACCACGCACAAGTGCCTCATTGCCGAGGCCTGGTGCTCTGTGCG
AGACCTGCCCGCCCTGCAGGAGGCCCTGCGGGACAGCTCGATGGAGGAGGGAGTGAGTGCCGTGGCTCAC
CGCATCCCCTGCCGGGACATGCCCCCCACACTCATCCGCACCAACCGCTTCACGGCCAGCTTCCAGGGCA
TCGTGGATGCCTACGGCGTGGGCCGCTACCAGGAGGTCAACCCCGCTCCCTACACCATCATCACCTTCCC
CTTCCTGTTTGCTGTGATGTTCGGGGATGTGGGCCACGGGCTGCTCATGTTCCTCTTCGCCCTGGCCATG
GTCCTTGCGGAGAACCGACCGGCTGTGAAGGCCGCGCAGAACGAGATCTGGCAGACTTTCTTCAGGGGCC
GCTACCTGCTCCTGCTTATGGGCCTGTTCTCCATCTACACCGGCTTCATCTACAACGAGTGCTTCAGTCG
CGCCACCAGCATCTTCCCCTCGGGCTGGAGTGTGGCCGCCATGGCCAACCAGTCTGGCTGGAGTGATGCA
TTCCTGGCCCAGCACACGATGCTTACCCTGGATCCCAACGTCACCGGTGTCTTCCTGGGACCCTACCCCT
TTGGCATCGATCCTATTTGGAGCCTGGCTGCCAACCACTTGAGCTTCCTCAACTCCTTCAAGATGAAGAT
GTCCGTCATCCTGGGCGTCGTGCACATGGCCTTTGGGGTGGTCCTCGGAGTCTTCAACCACGTGCACTTT
GGCCAGAGGCACCGGCTGCTGCTGGAGACGCTGCCGGAGCTCACCTTCCTGCTGGGACTCTTCGGTTACC
TCGTGTTCCTAGTCATCTACAAGTGGCTGTGTGTCTGGGCTGCCAGGGCCGCCTCGGCCCCCAGCATCCT
CATCCACTTCATCAACATGTTCCTCTTCTCCCACAGCCCCAGCAACAGGCTGCTCTACCCCCGGCAGGAG
GTGGTCCAGGCCACGCTGGTGGTCCTGGCCTTGGCCATGGTGCCCATCCTGCTGCTTGGCACACCCCTGC
ACCTGCTGCACCGCCACCGCCGCCGCCTGCGGAGGAGGCCCGCTGACCGACAGGAGGAAAACAAGGCCGG
GTTGCTGGACCTGCCTGACGCATCTGTGAATGGCTGGAGCTCCGATGAGGAAAAGGCAGGGGGCCTGGAT
GATGAAGAGGAGGCCGAGCTCGTCCCCTCCGAGGTGCTCATGCACCAGGCCATCCACACCATCGAGTTCT
GCCTGGGCTGCGTCTCCAACACCGCCTCCTACCTGCGCCTGTGGGCCCTGAGCCTGGCCCACGCCCAGCT
GTCCGAGGTTCTGTGGGCCATGGTGATGCGCATAGGCCTGGGCCTGGGCCGGGAGGTGGGCGTGGCGGCT
GTGGTGCTGGTCCCCATCTTTGCCGCCTTTGCCGTGATGACCGTGGCTATCCTGCTGGTGATGGAGGGAC
TCTCAGCCTTCCTGCACGCCCTGCGGCTGCACTGGGTGGAATTCCAGAACAAGTTCTACTCAGGCACGGG
CTACAAGCTGAGTCCCTTCACCTTCGCTGCCACAGATGACTAG


>OriGene 5' read for NM_006019 unedited
GAATTTGTAATACGACTCACTCATAGGGCGGCCGCGAATTCGCACGAGGCGGAGCGGGGC
AGCCAGCAGCGGAGGCGCGGCGCGCAGCACACCCGGGGACCATGGGCTCCATGTTCCGGA
GCGAGGAGGTGGCCCTGGTCCAGCTCTTTCTGCCCACAGCGGCTGCCTACACCTGCGTGA
GTCGGCTGGGCGAGCTGGGCCTCGTGGAGTTCAGAGACCTCAACGCCTCGGTGAGCGCCT
TCCAGAGACGCTTTGTGGTTGATGTTCGGCGCTGTGAGGAGCTGGAGAAGACCTTCACCT
TCCTGCAGGAGGAGGTGCGGCGGGCTGGGCTGGTCCTGCCCCCGCCAAAGGGGAGGCTGC
CGGCACCCCCACCCCGGGACCTGCTGCGCATCCAGGAGGAGACGGAGCGCCTGGCCCAGG
AGCTGCGGGATGTGCGGGGCAACCAGCAGGCCCTGCGGGCCCAGCTGCACCAGCTGCAGC
TCCACGCCGCCGTGCTACGCCAGGGCCATGAACCTCAGCTGGCAGCCGCCCACACAGATG
GGGCCTCAGAGAGGACGCCCCTGCTCCAGGCCCCCGGGGGGCCGCACCANGACCTGAGGG
TCAACTNTGTGGCATGTGCCGTGGAGCCCCACATGCCCCTGCCCTATAGCGCCTGCTCTG
GATGGCCTGCCGCGGCTTCCTCATTGCCAGCTTCAGGGAGCTGGAGCAGCCGCTGGAGCA
CCCCGTGACGATCGGACAGAAGATCCCGCAGATCACGGACTGCTTCCACTGCCACGTCTT
NCCGTTTCTGCAGCAGGAAGATGCCCGCCTCGGGGCCTGCAGCAGCTGCAACAGCAAAGC
CAGAGCTGGCANGAGGTCCCTCGGGGAGACAGAGCGGNTCCTGATCCCAGTGCTATGCCN
GGNTGCTGCATCTGCTGCCGCAGGGNCAGTGCANGTCCACAAATGAAAGGCGTGTACCTG
CCCCTGACATTGCATGTGAGCACAGCACATTGCCTCATGCGAGCCTGNGCTCTGGCAGAC
TGCCCGCTGAGAGCN
>OriGene 3' read for NM_006019 unedited
NNNNCCCAACTGTGAACCGCGGCTCGCAATCTAGGATCAGTTTTGTTTTATGGTTTATAT
TTTTGCCTTTTTTTGTTTTGTTTAGGGCAAAGGCGTACATCTTTATTCCTCTCCTGACTC
AAGAGTCAAGAAAGAGGTCTGGCAGGACCTGCAGTGGGCCCTAATCAACTGTGGCACCGA
AAGTGAAAGGACTCAACTTGTGACCCGTGCCTGAATAAAACTTGGTCTGGAATTCCACCC
CTTGCAACCCCAGGGCGTGCAAGAAGGCTGAAAATCCCTCCATCACCAGCCGGATAACCA
CGGGCATTACGGCTAAAGCCGCAAAAATGGGGACCAGTACCACAAGCGCCAAGCCCACCT
CCCGGCCCAGGGCCAAGCCTATGCGCATCACCATGGCCCACAAAACCTCGGACAACTGGG
CGTGGGCCAAGCTCAAGGCCCACAAGCACAAGNCAAGACGCTGTGTTGGAGACTCAGCCC
AACAAAACTTCAAGGTGTGGATTGCCTGTGCATGAGCACCTCGGGAGGGGAGACAAGCTG
GGGCTTCTTGTTATTATCCAAGCCCCCTGGTNNNTCTTTCCTATGGAGCCCCGGGCCTTT
TCCAAAATGGTAAGGAAGGGTCCCGCACCCCGGCCCTGTTTTCCCCCCCGCGGGCAAAGG
GCCCCCCTCCCAAAAAGGGGTTTAATGGAGGTTCCACACAGTTCCAGGTTTTCCCCCAAT
TCTTGTTGGGGCCCCATGGCAAAGGCCCGAACCCCACAGGGGGGCCGGGGCCCACCCCCC
CCCGGGGGGAGAAAAAACCCTTTTTTCGGGGGTTTGTGGCAAAAAAAAAAAACCTTTTAA
AAAAAGGGGTAAGAAATCCCCGGGGCCCCCAGGGGGGCCTTTTAACCCCCATAAAACAGA
GCCCTTTTTG
Restriction Sites NotI-NotI     
ACCN NM_006019
Insert Size 2700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006019.2, NP_006010.2
RefSeq Size 2700 bp
RefSeq ORF 2493 bp
Locus ID 10312
UniProt ID Q13488
Domains V_ATPase_sub_a
Protein Families Transmembrane
Protein Pathways Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
Gene Summary This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, May 2017]
Transcript Variant: This variant (1) encodes isoform a, also known as the OC116 protein because it is one of three V-ATPase 116 kD subunits. It is also referred to as the a3 isoform and is induced on osteoclast differentiation. This variant contains a unique 5' end and lacks an internal segment compared to variant 2, which results in an isoform (a) with a different N-terminus than that of isoform b.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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