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Nogo A (RTN4) (NM_153828) Human Untagged Clone
CAT＃: SC128187
RTN4 (untagged)-Human reticulon 4 (RTN4), transcript variant 2
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ASY; Nbla00271; Nbla10545; NI220/250; NOGO; NSP; NSP-CL; RTN-X; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC128187 sequence for NM_153828 edited (data generated by NextGen Sequencing)
ATGGAAGACCTGGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAG CCCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAGGAGGAAGAAGAGGAGGAG GAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCCCGCC GCCGGGCTGTCCGCGGCCCCAGTGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGATGGAC TTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCCCGTC GCCCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCA TCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCTGAGGACGACGAGCCTCCG GCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCAGGCAGAGCCCGTGTGGACC CCGCCAGCCCCGGCTCCCGCCGCGCCCCCCTCCACCCCGGCCGCGCCCAAGCGCAGGGGC TCCTCGGGCTCAGTGGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTG GTGTTTGGTGCCAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTA ACAGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAGCTTTAGGATATACAAGGGT GTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATCTGGAATCT GAAGTTGCTATATCTGAGGAGTTGGTTCAGAAGTACAGTAATTCTGCTCTTGGTCATGTG AACTGCACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTG AAGTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACA CTACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGTTATTTATGAACGGCATCAG GCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGCTAAA ATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAGCTGAATGA Clone variation with respect to NM_153828.2 >OriGene 5' read for NM_153828 unedited
TTGGATTTTGTAATACGACTCTCTATTAGGGCGGCCGCGAATTCGCACGAGCCACCCTCC AGCCATGGAAGACCTGGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCC GCAGCCCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAGGAGGAAGAAGAGGA GGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCC CGCCGCCGGGCTGTCCGCGGCCCCAGTGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGAT GGACTTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCC CGTCGCCCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGC GCCATCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCTGAGGACGACGAGCC TCCGGCCCGGGCCTCCCCCTTCTTCCCCGGNCAGNGNGAGCCCCCNAGCAGAGCCCGNGN GGACCCCGCCAGCCCCGGCTTCCGGCGNGCCCCCCNCCACCCCGGCCGCGCCCAAACGCA GGGGCNCCCCGGCCCCAGGGGNGGGGGACCCCCCGGACGGGAAAGACACAAAAAGACCGG AGGGGCGGCNGGNGCCCACCCAANCCCGCCGCCCACAGGCAGGACCCACGCAGGGGAGCG GAACAGCCCACAANGGCCGGGGCCCGGCCCCCGGACCAACAACCCGAAGGAAAACAGGGG GGGAACCAAGCGCCCCAAAAAAAAGAGGAGGGCCCCCCACCCGGGGCAAANNGGGACCCC GCAACGGCCCAACCCGAAGGGGGGGGCACAAAGACACCAAACCCGGCCCTGGACAAGGGA GCCGGCCAAAAAGCAACCCAGGCCCCCCNNCCAACGGCGACCCAACAGACCCCC >OriGene 3' read for NM_153828 unedited
GACTCGGATTTTTTTCTTTTTTTTTTTCTAGCTCCACCATCTCTGCAACTTGCCAANATG CGGCAAGACTATCTGCAACAAAGTAAAATATACAGGTTTTTTATTCCACCAGTGCCTCAG ATTAGATAGGAAAAAGATATGATTACGGTTTAAATCCATACATAGCAGCTTACAATACTT AAGATGATGAACACATGGCAGTCAAGACAGGTAATTTTTCCTCACAACAGTGCATGGCTA AAAATAAAGATCTAACAACGATCTGTGAAACTGCACTGCAACGTCAAGGTTCGTTCTTCC CTGACCCTCCCCCGTATAATCAAATGAATATCCCCTTTAAAGATGAACTCCTACTAATTA TTTTGGGCGTTTTCATTCAGCTTTGCGCTTCAATCCAGGGATTTTTGCTTGGATTTTAGC CATAGCATCTTTAACATTCTTATTTGCAAGTCCTAGATAATGATCTATCTGTGCCTGATG CCGTTCATAAATAACAGGGACACTGAAGAGTGAAAGGAGAGCCAAAATCAGTGGTGTCAG CCCATTAAACAAGGCCCCAACTTAGGGAAATACCCACATTAACCCTGCAAACTTTAGGGA ATCAACTTAATCATCCACTAAGAAGAGGCCCCTGGGTTCCTTTTTCGGGGCAGTTACCTG ACCCAGGGCGGAATTCCTGTACTTTTGAACCAACTCCTCAGATTTGGAACCTTAGATTCC CAGTTTTGCCCTGGAGGGGGGGGCCTTCAACTGGATTCTTGGGAAGGTTGGGACACACCC TTTGGTTTTCCAAAAGCTGTTGGGAACAGGAGGCAGGCCCAGGGAATGGTAGGCTGGAAC CCCCCCCAAGGTTGAATCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_153828 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153828.2, NP_722550.1 |
| RefSeq Size | 2414 bp |
| RefSeq ORF | 1122 bp |
| Locus ID | 57142 |
| UniProt ID | Q9NQC3 |
| Protein Families | Transmembrane |
| Gene Summary | This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2), also known as Foocen-M, Nogo-B, RTN-XS and RTN4-B1, lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (B), that is missing an internal segment, compared to isoform A. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219796 | RTN4 (Myc-DDK-tagged)-Human reticulon 4 (RTN4), transcript variant 2 |
CNY 5,856.00 |
|
| RC219796L1 | Lenti-ORF clone of RTN4 (Myc-DDK-tagged)-Human reticulon 4 (RTN4), transcript variant 2 |
CNY 8,256.00 |
|
| RC219796L2 | Lenti-ORF clone of RTN4 (mGFP-tagged)-Human reticulon 4 (RTN4), transcript variant 2 |
CNY 5,890.00 |
|
| RC219796L3 | Lenti-ORF clone of RTN4 (Myc-DDK-tagged)-Human reticulon 4 (RTN4), transcript variant 2 |
CNY 5,890.00 |
|
| RC219796L4 | Lenti-ORF clone of RTN4 (mGFP-tagged)-Human reticulon 4 (RTN4), transcript variant 2 |
CNY 5,890.00 |
|
| RG219796 | RTN4 (tGFP-tagged) - Human reticulon 4 (RTN4), transcript variant 2 |
CNY 4,370.00 |
