MAP1B (NM_005909) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FUTSCH; MAP5; PPP1R102; PVNH9
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_005909, the custom clone sequence may differ by one or more nucleotides ATGGCGACCGTGGTGGTGGAAGCCACCGAGCCGGAGCCGTCCGGCAGCATCGCCAACCCGGCGGCGTCCA CCTCGCCTAGCCTGTCGCACCGCTTCCTTGACAGCAAGTTCTACTTGCTGGTGGTCGTCGGCGAGATCGT GACCGAGGAGCACCTGCGGCGTGCCATCGGCAACATCGAGCTCGGAATCCGATCATGGGACACAAACCTG ATTGAATGCAACTTGGACCAAGAACTCAAACTTTTTGTATCTCGACACTCTGCAAGATTCTCTCCTGAAG TCCCAGGACAAAAGATCCTTCATCACCGAAGTGACGTTTTAGAAACAGTGGTCCTGATCAACCCTTCTGA TGAAGCAGTCAGCACCGAGGTGCGCTTAATGATCACTGATGCTGCCCGACACAAGCTGCTCGTGCTGACC GGGCAGTGCTTTGAAAATACCGGAGAGCTCATTCTCCAGTCCGGCTCTTTCTCCTTCCAGAACTTCATAG AGATTTTCACCGATCAAGAGATCGGGGAGTTACTAAGCACCACCCATCCTGCCAACAAAGCCAGCTTAAC CCTGTTCTGTCCTGAAGAAGGGGACTGGAAGAACTCCAATCTTGACAGACACAATCTCCAAGACTTCATC AATATTAAACTCAATTCAGCTTCTATCTTGCCAGAAATGGAAGGACTTTCTGAGTTTACCGAGTATCTCT CAGAATCAGTGGAAGTCCCATCTCCCTTTGACATCTTGGAACCTCCCACATCGGGTGGATTTCTGAAGCT CTCCAAGCCCTGCTGTTATATTTTTCCAGGAGGGAGGGGCGATTCTGCCTTGTTTGCAGTGAATGGTTTC AATATGCTCATCAATGGCGGATCAGAGAGAAAATCCTGCTTCTGGAAGCTCATCCGACACTTAGACCGAG TGGACTCCATCCTGCTCACCCACATTGGGGATGACAATTTGCCTGGAATAAACAGCATGTTACAGCGGAA AATTGCAGAGCTCGAGGAAGAACAGTCCCAGGGCTCCACCACAAATAGTGACTGGATGAAAAACCTCATC TCCCCTGACTTAGGAGTTGTATTTCTCAATGTACCTGAAAATCTCAAAAATCCAGAGCCAAACATCAAGA TGAAGAGAAGCATAGAAGAAGCCTGCTTCACTCTCCAGTACCTAAACAAATTGTCCATGAAACCAGAACC TCTGTTTAGAAGTGTAGGCAATACTATTGATCCTGTCATTCTTTTCCAAAAAATGGGAGTAGGTAAACTT GAGATGTATGTGCTTAATCCAGTCAAGAGCAGCAAGGAAATGCAGTATTTTATGCAGCAGTGGACTGGTA CCAACAAAGACAAGGCTGAATTCATTCTGCCTAATGGTCAAGAAGTAGATCTCCCGATTTCCTACTTAAC TTCAGTCTCATCTTTGATTGTGTGGCATCCAGCAAACCCTGCGGAGAAAATCATCCGAGTCCTGTTTCCT GGGAACAGCACCCAGTACAACATCCTGGAAGGGTTGGAAAAGCTCAAACATCTAGACTTTCTGAAGCAGC CACTGGCCACCCAAAAGGATCTCACTGGCCAGGTGCCCACTCCTGTGGTGAAACAAACAAAACTGAAACA GAGGGCTGATAGCCGAGAAAGTCTGAAGCCAGCCGCAAAACCACTTCCTAGCAAATCCGTGCGCAAGGAG TCAAAAGAAGAAACCCCTGAGGTCACAAAAGTGAATCACGTGGAAAAGCCACCCAAAGTTGAAAGCAAAG AAAAGGTAATGGTGAAAAAAGACAAGCCAATAAAAACAGAGACCAAACCTTCAGTGACTGAAAAGGAGGT TCCCAGCAAAGAAGAGCCATCTCCAGTGAAAGCCGAGGTGGCTGAGAAGCAAGCCACAGATGTCAAACCC AAAGCTGCCAAGGAGAAGACGGTGAAAAAGGAAACAAAGGTAAAGCCTGAAGACAAGAAAGAGGAGAAAG AAAAGCCAAAGAAAGAAGTGGCTAAAAAGGAGGACAAAACACCTATCAAGAAGGAGGAAAAACCAAAAAA GGAAGAGGTGAAAAAAGAAGTCAAAAAAGAGATCAAGAAAGAAGAGAAAAAAGAACCCAAGAAAGAGGTT AAGAAAGAAACACCGCCAAAGGAAGTCAAGAAGGAAGTTAAGAAGGAAGAGAAGAAGGAAGTGAAAAAGG AAGAAAAGGAACCCAAAAAAGAAATTAAGAAGCTCCCTAAAGACGCAAAGAAATCATCTACTCCTCTGTC TGAAGCAAAAAAACCAGCTGCTTTAAAACCAAAAGTACCCAAGAAGGAAGAGTCTGTCAAGAAAGATTCT GTTGCTGCCGGAAAGCCAAAGGAGAAGGGGAAAATAAAAGTCATTAAGAAGGAAGGCAAGGCCGCAGAGG CTGTCGCTGCAGCTGTCGGCACTGGAGCCACCACAGCAGCTGTCATGGCGGCAGCTGGAATAGCAGCCAT TGGCCCTGCCAAAGAACTCGAAGCTGAGAGGTCCCTTATGTCATCTCCTGAGGATCTAACCAAGGACTTT GAAGAGTTAAAGGCTGAAGAGGTCGATGTAACAAAGGACATCAAGCCTCAGCTGGAGCTAATCGAAGACG AAGAGAAACTGAAGGAAACTGAGCCAGTCGAAGCCTACGTCATCCAGAAGGAGAGAGAAGTCACCAAAGG TCCTGCCGAGTCCCCTGATGAGGGAATCACTACCACTGAAGGGGAGGGCGAATGTGAACAGACACCTGAG GAGCTGGAGCCCGTCGAGAAGCAGGGAGTAGACGACATTGAAAAATTTGAAGATGAAGGAGCCGGTTTTG AAGAATCTTCAGAGACTGGAGACTATGAAGAGAAGGCAGAAACTGAGGAGGCTGAGGAGCCAGAAGAGGA TGGGGAGGAACACGTATGTGTGAGCGCCTCCAAGCACAGCCCCACTGAGGATGAGGAAAGTGCCAAGGCG GAGGCTGATGCATACATCAGGGAGAAGAGGGAGTCTGTGGCCAGTGGGGATGACCGAGCCGAAGAAGACA TGGATGAGGCCATTGAGAAAGGAGAGGCTGAACAATCTGAAGAGGAGGCTGATGAGGAGGACAAAGCTGA AGATGCCAGAGAGGAGGAATATGAGCCGGAAAAAATGGAAGCTGAAGACTATGTGATGGCTGTGGTCGAC AAGGCTGCAGAGGCTGGTGGTGCCGAGGAGCAGTATGGATTCCTCACCACACCAACCAAGCAACTAGGAG CCCAGTCTCCTGGCCGAGAACCTGCATCTTCAATTCATGATGAGACTTTACCTGGAGGCTCAGAGAGCGA GGCCACCGCTTCTGATGAGGAGAATCGAGAAGACCAGCCTGAGGAATTCACTGCCACCTCTGGCTACACT CAGTCTACTATTGAGATATCCAGTGAGCCCACCCCCATGGATGAGATGTCTACCCCTCGAGACGTGATGA GTGATGAGACCAACAATGAAGAGACGGAGTCCCCTTCTCAGGAATTCGTAAATATCACCAAATATGAATC TTCATTGTATTCTCAGGAATACTCTAAACCTGCTGATGTTACACCGCTCAACGGATTTTCTGAAGGATCA AAAACAGATGCCACTGATGGCAAGGATTACAATGCTTCAGCCTCTACCATATCACCACCCTCTTCCATGG AGGAAGACAAATTCAGCAGATCTGCTTTACGTGATGCTTACTGCTCTGAAGTGAAAGCCAGCACCACTTT GGACATCAAAGATAGCATCTCAGCTGTTTCAAGTGAAAAGGTCAGCCCATCGAAGAGCCCGTCCCTGAGT CCATCTCCACCATCACCCTTAGAAAAGACCCCCCTGGGTGAACGTAGTGTGAACTTCTCTCTGACGCCCA ATGAGATTAAAGTCTCTGCAGAGGCAGAAGTAGCCCCGGTGTCTCCTGAGGTGACCCAAGAAGTAGTTGA AGAACATTGTGCTAGTCCTGAGGACAAGACTCTGGAAGTGGTGTCACCATCTCAGTCCGTGACTGGCAGT GCTGGTCACACACCTTACTATCAATCTCCTACTGACGAGAAATCCAGTCATCTCCCTACAGAAGTCATTG AAAAACCACCAGCAGTTCCAGTGAGTTTTGAATTCAGTGATGCCAAAGATGAGAATGAAAGGGCTTCAGT AAGCCCCATGGATGAGCCCGTGCCTGACTCAGAGTCTCCTATTGAAAAAGTTTTGTCTCCTTTACGCAGC CCGCCCCTCATTGGATCCGAGTCTGCTTATGAAAGTTTTCTAAGTGCTGATGACAAGGCTTCTGGCAGAG GTGCCGAAAGTCCTTTTGAAGAAAAGAGTGGAAAACAAGGCTCTCCAGACCAAGTAAGTCCAGTTTCTGA AATGACTTCTACTAGTCTTTACCAAGACAAACAGGAAGGGAAAAGCACAGACTTTGCACCAATAAAAGAA GACTTTGGCCAAGAAAAGAAAACTGATGATGTTGAAGCCATGAGTTCTCAACCAGCACTGGCTCTGGATG AAAGGAAATTAGGAGATGTTTCTCCCACACAAATAGATGTCAGTCAGTTTGGATCTTTTAAAGAAGACAC TAAGATGTCCATTTCTGAAGGTACTGTCTCAGACAAGTCAGCTACTCCTGTTGATGAGGGCGTAGCAGAA GACACGTACTCTCATATGGAGGGTGTGGCCTCAGTGTCCACAGCCTCAGTGGCTACGAGCTCATTTCCAG AGCCAACAACAGATGATGTGTCTCCATCTCTGCATGCTGAGGTTGGCTCCCCACATTCCACAGAAGTAGA TGACTCCCTTTCAGTGTCTGTTGTGCAAACACCTACCACATTCCAGGAAACAGAAATGTCTCCATCTAAA GAAGAATGCCCAAGACCGATGTCAATTTCTCCACCAGATTTCTCCCCTAAAACTGCAAAGTCCAGGACAC CCGTTCAAGATCACAGATCTGAACAGTCCTCAATGTCTATTGAATTTGGCCAAGAATCTCCTGAGCAATC CCTTGCTATGGACTTCAGTCGACAGTCTCCAGATCACCCTACAGTGGGTGCAGGCGTGCTTCACATCACT GAAAATGGGCCAACTGAAGTGGACTACAGTCCTTCTGACATGCAGGACTCCAGTTTATCACATAAGATAC CACCTATGGAGGAGCCGTCCTACACCCAAGATAATGATCTTTCTGAGCTCATCTCAGTATCTCAGGTAGA GGCCTCCCCGTCCACCTCTTCTGCTCATACCCCTTCTCAGATCGCTTCTCCTCTCCAAGAAGATACTCTA TCCGATGTTGCTCCTCCCAGAGATATGTCCTTATATGCCTCACTCACCTCTGAAAAAGTGCAAAGTCTGG AAGGAGAGAAGCTCTCTCCAAAATCTGATATCTCTCCACTCACCCCACGAGAGTCCTCTCCTTTATATTC ACCTACTTTTTCAGATTCTACCTCTGCAGTCAAAGAGAAAACAGCAACTTGCCACAGTTCCTCTTCTCCA CCAATAGATGCAGCATCCGCAGAGCCCTATGGCTTCCGTGCCTCAGTGTTATTCGATACAATGCAACACC ATCTAGCCTTGAATAGAGATTTGTCCACACCTGGCCTGGAGAAGGACAGTGGAGGGAAGACACCTGGTGA CTTTAGCTATGCCTATCAAAAGCCTGAGGAAACAACCAGGTCCCCAGATGAAGAAGATTATGACTATGAG TCTTATGAGAAGACCACCCGGACCTCAGATGTGGGTGGCTATTACTATGAGAAGATAGAGAGAACCACAA AATCTCCAAGTGACAGTGGCTACTCCTATGAGACCATTGGGAAAACTACCAAGACCCCTGAAGATGGTGA CTATTCCTATGAAATTATTGAGAAGACCACACGGACCCCTGAAGAGGGTGGGTACTCATATGACATAAGT GAAAAGACCACCAGCCCCCCCGAAGTGAGTGGTTACAGCTATGAAAAGACTGAGAGGTCTAGAAGGCTTC TGGATGACATCAGCAATGGCTATGATGACTCTGAGGATGGTGGCCACACACTTGGGGACCCCAGCTACTC TTATGAAACCACTGAGAAAATTACCAGTTTCCCTGAGTCTGAAGGTTATTCCTATGAGACATCTACAAAG ACAACACGAACCCCTGATACTTCCACATACTGTTACGAGACTGCAGAGAAAATCACTAGAACCCCTCAGG CATCCACATATTCCTACGAGACTTCAGACCTATGCTACACTGCAGAAAAGAAGTCCCCCTCAGAAGCCCG TCAGGATGTCGATTTATGCCTCGTGTCCTCTTGTGAATACAAGCACCCCAAGACAGAGCTTTCACCCTCT TTCATTAATCCCAATCCTCTTGAGTGGTTTGCCAGTGAAGAACCCACTGAAGAATCTGAAAAGCCCCTCA CTCAATCAGGGGGAGCCCCACCGCCTCCAGGAGGAAAGCAACAGGGCCGACAGTGTGATGAAACCCCTCC CACCTCAGTCAGCGAGTCAGCCCCATCCCAGACCGACTCTGATGTTCCCCCGGAGACTGAAGAGTGCCCC TCCATCACGGCCGATGCCAATATCGACTCTGAAGACGAGTCGGAAACCATCCCCACAGACAAAACTGTCA CGTACAAACACATGGACCCACCTCCAGCTCCCGTGCAAGACCGCAGCCCTTCGCCACGCCACCCTGATGT GTCCATGGTGGACCCAGAGGCCTTGGCCATTGAGCAGAACCTGGGCAAAGCTCTAAAGAAAGATCTGAAA GAGAAGACCAAAACCAAAAAGCCAGGTACAAAGACCAAGTCATCTTCACCTGTCAAAAAGAGTGATGGGA AGTCTAAGCCCTTGGCAGCTTCACCAAAACCAGCGGGCTTGAAAGAATCCTCGGATAAAGTGTCCAGGGT GGCTTCTCCTAAGAAGAAAGAATCTGTGGAAAAGGCAGCAAAACCCACCACCACTCCTGAGGTCAAAGCT GCACGTGGGGAAGAGAAAGACAAGGAGACCAAGAATGCTGCCAATGCCTCTGCATCCAAGTCGGCCAAGA CCGCCACTGCAGGACCAGGAACTACCAAGACGACCAAGTCATCTGCTGTGCCCCCAGGCCTCCCTGTGTA TTTGGACCTGTGCTACATTCCTAACCACAGCAATAGTAAGAATGTTGATGTGGAATTTTTCAAGAGAGTG CGGTCTTCCTACTACGTGGTGAGTGGGAATGACCCTGCTGCTGAGGAGCCCAGCCGGGCTGTCCTGGACG CTTTGTTGGAAGGAAAGGCTCAGTGGGGCAGCAACATGCAGGTGACACTGATCCCAACTCATGACTCAGA AGTGATGAGGGAATGGTACCAGGAGACCCATGAGAAACAGCAAGATCTCAACATCATGGTTTTAGCAAGC AGCAGCACAGTGGTTATGCAAGATGAATCCTTCCCTGCATGCAAGATTGAACTGTAA
Restriction Sites	Please inquire
ACCN	NM_005909
Insert Size	10000 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	A TrueClone.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_005909.2, NP_005900.1
RefSeq Size	9416 bp
RefSeq ORF	7407 bp
Locus ID	4131
UniProt ID	P46821
Domains	MAP1B_neuraxin
Gene Summary	This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) lacks exon 3A as compared to variant 2. It contains alternate exons, 1 and 2, which results in an alternate 5' UTR sequence and starting methionine. The encoded protein is thus longer at the N-terminus but otherwise id

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