CUG BP1 (CELF1) (NM_006560) Human Untagged Clone

Name: CUG BP1 (CELF1) (NM_006560) Human Untagged Clone
SKU: SC128043
Price: 3656.00

CAT＃: SC128043

CELF1 (untagged)-Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 1

Datasheet

SDS

"NM_006560" in other vectors (6)

CNY 3,656.00

货期*

现货

规格

Cited in 5 publications.

Product images

经常一起买 (4)

TA500951

Anti-CUGBP1 mouse monoclonal antibody, clone OTI5B8 (formerly 5B8)

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP; hNab50; NAB50; NAPOR
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC128043 sequence for NM_006560 edited (data generated by NextGen Sequencing) ATGAACGGCACCCTGGACCACCCAGACCAACCAGATCTTGATGCTATCAAGATGTTTGTG GGCCAGGTTCCAAGGACCTGGTCTGAAAAGGACTTGCGGGAACTCTTCGAACAGTATGGT GCTGTGTATGAAATCAACGTCCTAAGGGATAGGAGCCAAAACCCGCCTCAGAGCAAAGGG TGCTGTTTTGTTACATTTTACACCCGTAAAGCTGCATTAGAAGCTCAGAATGCTCTTCAC AACATGAAAGTCCTCCCAGGGATGCATCACCCTATACAGATGAAACCTGCTGACAGTGAG AAGAACAATGCAGTGGAAGACAGGAAGCTGTTTATTGGTATGATTTCCAAGAAGTGCACT GAAAATGACATCCGAGTCATGTTCTCTTCGTTTGGACAGATTGAAGAATGCCGGATATTG CGGGGACCTGATGGCCTGAGCCGAGGTTGTGCATTTGTGACTTTTACAACAAGAGCCATG GCACAGACGGCTATCAAGGCAATGCACCAAGCACAGACCATGGAGGGTTGCTCATCACCC ATGGTGGTAAAATTTGCTGATACACAGAAGGACAAAGAACAGAAGAGAATGGCCCAGCAG CTCCAGCAGCAGATGCAGCAAATCAGCGCAGCATCTGTGTGGGGAAACCTTGCTGGTCTA AATACTCTTGGACCCCAGCATTTAGCACTCCTTCAGCAGACTGCCTCCTCTGGGAACCTC AACACCCTGAGCAGCCTCCACCCAATGGGAGGGTTGAATGCAATGCAGTTACAGAATTTG GCTGCACTAGCTGCTGCAGCTAGTGCAGCTCAGAACACACCAAGTGGTACCAATGCTCTC ACTACATCCAGCAGTCCCCTCAGCGTGCTCACTAGTTCAGGGTCCTCACCTAGCTCTAGC AGCAGTAATTCTGTCAACCCCATAGCCTCACTTGGAGCCCTGCAGACATTAGCTGGAGCA ACGGCTGGCCTCAATGTTGGCTCTTTGGCAGGAATGGCTGCTTTAAATGGTGGCCTGGGC AGCAGTGGCCTTTCCAATGGCACCGGGAGCACCATGGAGGCCCTCACTCAGGCCTACTCG GGTATCCAGCAATATGCTGCTGCTGCGCTCCCCACTCTGTACAACCAGAATCTTCTGACA CAGCAGAGTATTGGTGCTGCTGGAAGCCAGAAGGAAGGTCCAGAGGGAGCCAACCTGTTC ATCTACCACCTGCCCCAGGAGTTTGGTGATCAGGACCTGCTGCAGATGTTTATGCCCTTT GGGAATGTCGTGTCTGCCAAGGTTTTCATAGACAAGCAGACAAACCTGAGCAAGTGTTTT GGTTTTGTAAGTTACGACAATCCTGTTTCGGCCCAAGCTGCCATCCAGTCCATGAACGGC TTTCAGATTGGCATGAAGCGGCTTAAAGTGCAGCTCAAACGTTCGAAGAATGACAGCAAG CCCTACTGA Clone variation with respect to NM_006560.3 679 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_006560 unedited GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGCGGCAGCGGCAGC GGCGGCGGGACGCGGAGGCTCCCCCGGGATTCGGCCTCAGCAGCGAGGCGGCGGCGGCGG CTGCGGAGGCGCAGGCAGCAACTGAGGCAGCGGCAGGCTCAGGTGCAGCCGCTGGCTGGA GTGCAGTGGTGTGTTCTCAGCTCTGCAGCCCCCGCTTCCCAGGTTCAAGTAACTGCCTCA GCCTTCTGAGTAGCTGGGATTACAGGTGTGCACCACCACGCCCAGGCTGAACATAACTTC TTTTTTTCAAAGTTCCCTGTGTTGTGAACTGAGCTTGGGTGAAGTGAAATCTTTGAGGAA GCTAAAGCTGTGTGTATTTGTGGATCAGACAAGTGCAGCAAGTTAATATCATTGGCTTCT GAAGGGGAGAGTGAGGTGATGGCTGCGTTTAAGTTGGATTTCCTTCCAGAAATGATGGTG GATCATTGCTCTTTGAATTCCAGTCCCGTCTCAAAGAAAATGAACGGCACCCTGGACCAC CCAGACCAACCAGATCTTGATGCTATCAAGATGTTTGTGGGCCAGGTTCCAAGGACCTGG TCTGAAAAGGACTTGCGGGAACTCTTCGAACAGTATGGTGCTGTGTATGAAATCAACGTC CTAAGGGATAGGAGCCAAAACCCGCCTCAGACANAGGGTGCTGTTTTGTTACATTTTACA NCCCGTAAGCTGCATTAGAAGCTCAGAATGCTCTTCACACATGAAAGTCCTCCCAGGATG CATCACCCTATACAGATGANACCTGCTGACAGTGAGAAGAACAATGCAGTGGNAAGACAG AAAGCTGTTNATTGGTATGANTTCCCAGAAGTGCACTGANAATGACATNNCGAGTCATGT CTCTTCGTTNGGAANNGATGAANAATGCCGNATATTTGCGGGACCTGATGGCCTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' genomic read for NM_006560 unedited GTACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATTTTTATTTTTTG CATTTATTTAAAAAATCCCATCATGACCCTGGAAGCCTTTAGAACAGTTTTATCCTTTGA AACACAGGACACATTTCTCCCAGTGCGCAGAATTTCAAGTTTACGTGGTTCAGCTTAAGA AGTGTATGTTTCACGTTCCTTAGAGGACAACAGACCCAAGTTATCACTATGAGAAAGGAA CAGCTGTCCCAGCTTCAATGGGATAATCCAACACCACCAGCTACCTGTACAACAGTAAGA TGGTCAATCCCTGTCTGTTACCCACAGGGACAGCATGACAAGGAGAGAGCCCCCATCTGA CTTAATAGCAAACCAACCCCTTTATTCTTATCTCTGCTGCTAGGGGTCAAGACTGGTATT TGTTGAGGTTTCAGATTCCAGAGACCCAGGGATCCCATCCCCTGGCTGTGGCTACAGTCA CATCTTAACAGGGCCACCTCTGCCCAGGTGTACATTCCCAACAGCATGTCCTTCCTGGTT CTCTACCCCCACAGCACTTCTTAGAGCACAGGCCGAGCCCACAAGCTGTGTGGGTCACAG GCAAGAGCTGAAGTAAGACCTGCAAGAGGCGGCAGGGAGCTAACTGTACCACGAGCACAA AACGACCCGGTTATACTGAGGTAAACGAACACTCACTTCATGCGGAGGCTGGCAACGCCC TGATGTTCCCTCTGCCTCAGAGCAAACAAGGGATTGGAGTGTTTCCGGAAGGCCCTGCCA CACCCCTGGGGAAATCTACTCTGGGCACCTAATTAACGAGGCAGGGAAACCTCTGGCACT GGCACCGACCCTCTGGTTCGCCGGACCCTCGGTTAAGTCCCCAAAAAAACCCCCCCTCCC CATACAGGTTCGGCCCCTCCCCCAACGCCCCG
Restriction Sites	NotI-NotI
ACCN	NM_006560
Insert Size	3780 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_006560.2, NP_006551.1
RefSeq Size	2157 bp
RefSeq ORF	1449 bp
Locus ID	10658
UniProt ID	Q92879
Domains	RRM
Protein Families	Druggable Genome
Gene Summary	Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the predominant transcript and it encodes isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (5)

The use of this cDNA Clones has been cited in the following citations:
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition ,Yan, JK;Zhang, T;Dai, LN;Gu, BL;Zhu, J;Yan, WH;Cai, W;Wang, Y;, FASEB J. ,PubMed ID 30514107 [CELF1]
Overexpression of miR-214-3p in esophageal squamous cancer cells enhances sensitivity to cisplatin by targeting survivin directly and indirectly through CUG-BP1 ,Phatak, P;Byrnes, KA;Mansour, D;Liu, L;Cao, S;Li, R;Rao, JN;Turner, DJ;Wang, JY;Donahue, JM;, Oncogene ,PubMed ID 26234674 [CELF1]
Competition between RNA-binding proteins CELF1 and HuR modulates MYC translation and intestinal epithelium renewal ,Liu, L;Ouyang, M;Rao, JN;Zou, T;Xiao, L;Chung, HK;Wu, J;Donahue, JM;Gorospe, M;Wang, JY;, Mol. Biol. Cell ,PubMed ID 25808495 [CELF1]
Jnk2 Deletion Disrupts Intestinal Mucosal Homeostasis and Maturation by Differentially Modulating RNA-binding Proteins HuR and CUGBP1 ,Chung, HK;Rao, JN;Zou, T;Liu, L;Xiao, L;Gu, H;Turner, DJ;Yang, P;Wang, JY;, Am. J. Physiol., Cell Physiol. April 2014 ,PubMed ID 24740539 [CELF1]
Competitive binding of CUGBP1 and HuR to occludin mRNA controls its translation and modulates epithelial barrier function ,Ting-Xi Yu, Jaladanki N. Rao, Tongtong Zou, Lan Liu, Lan Xiao, Miao Ouyang, Shan Cao, Myriam Gorospe, and Jian-Ying Wang, Mol. Biol. Cell, Jan 2013; 24: 85 - 99. [CELF1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC206275	CELF1 (Myc-DDK-tagged)-Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 3,990.00
RC206275L1	Lenti ORF clone of Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,056.00
RC206275L2	Lenti ORF clone of Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC206275L3	Lenti ORF clone of Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,056.00
RC206275L4	Lenti ORF clone of Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG206275	CELF1 (tGFP-tagged) - Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,256.00

其它CUG BP1产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies