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PNK (PNKP) (NM_007254) Human Untagged Clone
CAT＃: SC128040
PNKP (untagged)-Human polynucleotide kinase 3'-phosphatase (PNKP)
CNY 3,888.00
| Cited in 1 publication. |
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AOA4; CMT2B2; EIEE10; MCSZ; PNK |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC128040 sequence for NM_007254 edited (data generated by NextGen Sequencing)
ATGGGCGAGGTGGAGGCCCCGGGCCGCTTGTGGCTCGAGAGCCCCCCTGGGGGAGCGCCC CCCATCTTCCTGCCCTCGGACGGGCAAGCCCTGGTCCTGGGCAGGGGACCCCTGACCCAG GTTACGGACCGGAAGTGCTCCAGAACTCAAGTGGAGCTGGTCGCAGATCCTGAGACCCGG ACAGTGGCAGTGAAACAGCTGGGAGTTAACCCCTCAACTACCGGGACCCAGGAGTTGAAG CCGGGGTTGGAGGGCTCTCTGGGGGTGGGGGACACACTGTATTTGGTCAATGGCCTCCAC CCACTGACCCTGCGCTGGGAAGAGACCCGCACACCAGAATCCCAGCCAGATACTCCGCCT GGCACCCCTCTGGTGTCCCAAGATGAGAAGAGAGATGCTGAGCTGCCGAAGAAGCGTATG CGGAAGTCAAACCCCGGCTGGGAGAACTTGGAGAAGTTGCTAGTGTTCACCGCAGCTGGG GTGAAACCCCAGGGCAAGGTGGCTGGCTTTGATCTGGACGGGACGCTCATCACCACACGC TCTGGGAAGGTCTTTCCCACTGGCCCCAGTGACTGGAGGATCTTGTACCCAGAGATTCCC CGTAAGCTCCGAGAGCTGGAAGCCGAGGGCTACAAGCTGGTGATCTTCACCAACCAGATG AGCATCGGGCGCGGGAAGCTGCCAGCCGAGGAGTTCAAGGCCAAGGTGGAGGCTGTGGTG GAGAAGCTGGGGGTCCCCTTCCAGGTGCTGGTGGCCACGCACGCAGGCTTGTACCGGAAG CCGGTGACGGGCATGTGGGACCATCTGCAGGAGCAGGCCAACGACGGCACGCCCATATCC ATCGGGGACAGCATCTTTGTGGGAGACGCAGCCGGACGCCCGGCCAACTGGGCCCCGGGG CGGAAGAAGAAAGACTTCTCCTGCGCCGATCGCCTGTTTGCCCTCAACCTTGGCCTGCCC TTCGCCACGCCTGAGGAGTTCTTTCTCAAGTGGCCAGCAGCCGGCTTCGAGCTCCCAGCC TTTGATCCGAGGACTGTCTCCCGCTCAGGGCCTCTCTGCCTCCCCGAGTCCAGGGCCCTC CTGAGCGCCAGCCCGGAGGTGGTTGTCGCAGTGGGATTCCCTGGGGCCGGGAAGTCCACC TTTCTCAAGAAGCACCTCGTGTCGGCCGGATATGTCCACGTGAACAGGGACACGCTAGGC TCCTGGCAGCGCTGTGTGACCACGTGTGAGACAGCCCTGAAGCAAGGGAAACGGGTCGCC ATCGACAACACAAACCCAGACGCCGCGAGCCGCGCCAGGTACGTCCAGTGTGCCCGAGCC GCGGGCGTCCCCTGCCGCTGCTTCCTCTTCACCGCCACTCTGGAGCAGGCGCGCCACAAC AACCGGTTTCGAGAGATGACGGACTCCTCTCATATCCCCGTGTCAGACATGGTCATGTAT GGCTACAGGAAGCAGTTCGAGGCCCCAACGCTGGCTGAAGGCTTCTCTGCCATCCTGGAG ATCCCGTTCCGGCTATGGGTGGAGCCGAGGCTGGGGCGGCTGTACTGCCAGTTCTCCGAG GGCTGA Clone variation with respect to NM_007254.3 >OriGene 5' read for NM_007254 unedited
ATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACCAGGGGGAGGGATGGA GCACGCGTCGTCGCCTGGGAAACGGGTCGACCCGCGGAAGGCGAGCGGGTGGGACTTCCG GAGCAGTTAATGGCCGGCACCCAGGATGGGCGAGGTGGAGGCCCCGGGCCGCTTGTGGCT CGAGAGCCCCCCTGGGGGAGCGCCCCCCATCTTCCTGCCCTCGGACGGGCAAGCCCTGGT CCTGGGCAGGGGACCCCTGACCCAGGTTACGGACCGGAAGTGCTCCAGAACTCAAGTGGA GCTGGTCGCAGATCCTGAGACCCGGACAGTGGCAGTGAAACAGCTGGGAGTTAACCCCTC AACTACCGGGACCCAGGAGTTGAAGCCGGGGTTGGAGGGCTCTCTGGGGGTGGGGGACAC ACTGTATTTGGTCAATGGCCTCCACCCACTGACCCTGCGCTGGGAAGAGACCCGCACACC AGAATCCCAGCCAGATACTCCGCCTGGCACCCCTCTGGTGTCCCAAGATGAGAAGAGAGA TGCTGAGCTGCCGAAGAAGCGTATGCGGAAGTCAAACCCCGGCTGGGAGAACTTGGAGAA GTTGCTAGTGTTCACCGCAGCTGGGGTGAAACCCNCAGGCAGGGTGGCTGGCTTTGATCT GGACGGGACGCTCATCACCACACGCTCTGGGAAGGTCTTTCCCACTGGCCCCAGTGACTG GAGGGATCTGTACCCAGAGATTCCCCCGTAGCTCCGAGAGCTGNAAGCCGAGGGCTACAA GCTGGTGATCTTNNCACACCAGATGAGCATCGGGCGCGGGAAGCTGCCCGCCCGAGAGTT CAAGGCCCAAGTGNNAGCTGTGGTTGAAAAACTGGGGGTTCCCTTTCAGGTGCTGTGGGC ACCCCACCAGGCTTGAACCGAAACCCGTAACCGCCATTGGGAACATTTTGAGAACAGGCC ACCACGGGCCCCCCATTCCTTCGGGAA >OriGene 3' read for NM_007254 unedited
NAAGGCGTACTATGNACGCGGCCGCTATCTANGATCGGTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGAAAAAAAGCGTTTATTGGGGAGGGGAG CTGGGCGGGGCTCACCCCTCGGAAAACTGGGAGTACAGCCGCCCCAGCCTGGGTTCCACC CATAGCCGGAACGGGATCTCCAGGATGGCAAAAAACCCTTCACCCAGCGTTGGGGCCTCA AACTGTTTCCTGGAGCCATACATGACCATGTTTGACACGGGGATATGAAAGGAGTCCGTC ATCTTTCAAAACCGGTTGTTGGGGCCCCCCTGCTCCAAAGGGGCGGGGAAAAGGAAACAG CGGCAGGGGACCCCCGGGGCTCGGGCACACTGGACGTACCTGGCGCGGTTCGGGGCGTTT GGGTTTGGGTTGTCAATGGCAACCCGTTTCCCTTGCTTAAGGGCTGTCTCACACGTGGTC ACACAGGGCTGCCAGGAGCCTAACGGGTCCCTGTTCACGTGGACATATCCGGCCGACACG AGGGGCTTCTTGAGAAAGGGGGACTTCCCGGCCCCAGGGAATCCCACTGGGACAACCACC TCCGGGCTGGCGCTCAGGAGGGCCCTGGACTCGGGGAGGCATAAAGGCCCTGAGCGGGAA ACAGCCCTCGGATCAAAGGCTGGGAGCTCGAACCCGGCTGCTGGCCACTTGAAAAAGTAC TCCTCNAGCGTGGGCAAAGGGGAGGCCAAGGTTGAGGGCAAAAAGGGCGATCGGCGGCAG GAAAAATCTTTCTTCTTTCCGCCCCGGGCCCCAATTGGCCCGGCGTCCCGGTTGGGGTTT CCAAAAAAAAAGCTGTCCCCCATGGGATATGGGGCG |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_007254 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007254.2, NP_009185.2 |
| RefSeq Size | 1745 bp |
| RefSeq ORF | 1566 bp |
| Locus ID | 11284 |
| UniProt ID | Q96T60 |
| Protein Families | Druggable Genome, Phosphatase |
| Gene Summary | This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Phosphorylation of polynucleotide kinase/ phosphatase by DNA-dependent protein kinase and ataxia-telangiectasia mutated regulates its association with sites of DNA damage
,Angela E. Zolner, Ismail Abdou, Ruiqiong Ye, Rajam S. Mani, Mesfin Fanta, Yaping Yu, Pauline Douglas, Nasser Tahbaz, Shujuan Fang, Tracey Dobbs, Chen Wang, Nick Morrice, Michael J. Hendzel, Michael Weinfeld, and Susan P. Lees-Miller,
Nucleic Acids Res., Nov 2011; 39: 9224 - 9237.
[PNKP]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207551 | PNKP (Myc-DDK-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 3,880.00 |
|
| RC207551L1 | Lenti-ORF clone of PNKP (Myc-DDK-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,280.00 |
|
| RC207551L2 | Lenti-ORF clone of PNKP (mGFP-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,460.00 |
|
| RC207551L3 | Lenti-ORF clone of PNKP (Myc-DDK-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,280.00 |
|
| RC207551L4 | Lenti-ORF clone of PNKP (mGFP-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,460.00 |
|
| RG207551 | PNKP (tGFP-tagged) - Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 5,480.00 |
|
| SC323976 | PNKP (untagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 3,888.00 |
