PNK (PNKP) (NM_007254) Human Untagged Clone
CAT#: SC323976
PNKP (untagged)-Human polynucleotide kinase 3'-phosphatase (PNKP)
CNY 3,888.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | AOA4; CMT2B2; EIEE10; MCSZ; PNK |
Vector | pCMV6-AC |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>OriGene sequence for NM_007254.2
GGAACCGACCGCCGCCGGCCGGGTTGCAGGCGGGGCACCTCGGGCAGGACCTCCCTGGTC
GGAAGTGGCCGTGAGCCCAAGCCGCGGTCCCGGGCCGGCACCCAGGATGGGCGAGGTGGA GGCCCCGGGCCGCTTGTGGCTCGAGAGCCCCCCTGGGGGAGCGCCCCCCATCTTCCTGCC CTCGGACGGGCAAGCCCTGGTCCTGGGCAGGGGACCCCTGACCCAGGTTACGGACCGGAA GTGCTCCAGAACTCAAGTGGAGCTGGTCGCAGATCCTGAGACCCGGACAGTGGCAGTGAA ACAGCTGGGAGTTAACCCCTCAACTACCGGGACCCAGGAGTTGAAGCCGGGGTTGGAGGG CTCTCTGGGGGTGGGGGACACACTGTATTTGGTCAATGGCCTCCACCCACTGACCCTGCG CTGGGAAGAGACCCGCACACCAGAATCCCAGCCAGATACTCCGCCTGGCACCCCTCTGGT GTCCCAAGATGAGAAGAGAGATGCTGAGCTGCCGAAGAAGCGTATGCGGAAGTCAAACCC CGGCTGGGAGAACTTGGAGAAGTTGCTAGTGTTCACCGCAGCTGGGGTGAAACCCCAGGG CAAGGTGGCTGGCTTTGATCTGGACGGGACGCTCATCACCACACGCTCTGGGAAGGTCTT TCCCACTGGCCCCAGTGACTGGAGGATCTTGTACCCAGAGATTCCCCGTAAGCTCCGAGA GCTGGAAGCCGAGGGCTACAAGCTGGTGATCTTCACCAACCAGATGAGCATCGGGCGCGG GAAGCTGCCAGCCGAGGAGTTCAAGGCCAAGGTGGAGGCTGTGGTGGAGAAGCTGGGGGT CCCCTTCCAGGTGCTGGTGGCCACGCACGCAGGCTTGTACCGGAAGCCGGTGACGGGCAT GTGGGACCATCTGCAGGAGCAGGCCAACGACGGCACGCCCATATCCATCGGGGACAGCAT CTTTGTGGGAGACGCAGCCGGACGCCCGGCCAACTGGGCCCCGGGGCGGAAGAAGAAAGA CTTCTCCTGCGCCGATCGCCTGTTTGCCCTCAACCTTGGCCTGCCCTTCGCCACGCCTGA GGAGTTCTTTCTCAAGTGGCCAGCAGCCGGCTTCGAGCTCCCAGCCTTTGATCCGAGGAC TGTCTCCCGCTCAGGGCCTCTCTGCCTCCCCGAGTCCAGGGCCCTCCTGAGCGCCAGCCC GGAGGTGGTTGTCGCAGTGGGATTCCCTGGGGCCGGGAAGTCCACCTTTCTCAAGAAGCA CCTCGTGTCGGCCGGATATGTCCACGTGAACAGGGACACGCTAGGCTCCTGGCAGCGCTG TGTGACCACGTGTGAGACAGCCCTGAAGCAAGGGAAACGGGTCGCCATCGACAACACAAA CCCAGACGCCGCGAGCCGCGCCAGGTACGTCCAGTGTGCCCGAGCCGCGGGCGTCCCCTG CCGCTGCTTCCTCTTCACCGCCACTCTGGAGCAGGCGCGCCACAACAACCGGTTTCGAGA GATGACGGACTCCTCTCATATCCCCGTGTCAGACATGGTCATGTATGGCTACAGGAAGCA GTTCGAGGCCCCAACGCTGGCTGAAGGCTTCTCTGCCATCCTGGAGATCCCGTTCCGGCT ATGGGTGGAGCCGAGGCTGGGGCGGCTGTACTGCCAGTTCTCCGAGGGCTGAGCCCCGCC CAGCTCCCCTCCACAATAAACGCTGTTTCTCCTTGAAAAAAAAAAAAAAAAAAAAAAAAA AAAAA |
Restriction Sites | ECoRI-NOT |
ACCN | NM_007254 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_007254.2, NP_009185.2 |
RefSeq Size | 1745 bp |
RefSeq ORF | 1566 bp |
Locus ID | 11284 |
UniProt ID | Q96T60 |
Protein Families | Druggable Genome, Phosphatase |
Gene Summary | This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207551 | PNKP (Myc-DDK-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 3,880.00 |
|
RC207551L1 | Lenti-ORF clone of PNKP (Myc-DDK-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,280.00 |
|
RC207551L2 | Lenti-ORF clone of PNKP (mGFP-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,460.00 |
|
RC207551L3 | Lenti-ORF clone of PNKP (Myc-DDK-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,280.00 |
|
RC207551L4 | Lenti-ORF clone of PNKP (mGFP-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,460.00 |
|
RG207551 | PNKP (tGFP-tagged) - Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 5,480.00 |
|
SC128040 | PNKP (untagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 3,888.00 |