CDC14B (NM_003671) Human Untagged Clone
CAT#: SC128030
CDC14B (untagged)-Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 1
CNY 7,320.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | Cdc14B1; Cdc14B2; CDC14B3; hCDC14B |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_003671, the custom clone sequence may differ by one or more nucleotides
ATGAAGCGGAAAAGCGAGCGGCGGTCGAGCTGGGCCGCCGCGCCCCCCTGCTCGCGGCGCTGCTCGTCGA CCTCGCCGGGTGTGAAGAAGATCCGCAGCTCCACGCAGCAAGACCCGCGCCGCCGGGACCCCCAGGACGA CGTGTACCTGGACATCACCGATCGCCTTTGTTTTGCCATTCTCTACAGCAGACCAAAGAGTGCATCAAAT GTACATTATTTCAGCATAGATAATGAACTTGAATATGAGAACTTCTACGCAGATTTTGGACCACTCAATC TGGCAATGGTTTACAGATATTGTTGCAAGATCAATAAGAAATTAAAGTCCATTACAATGTTAAGGAAGAA AATTGTTCATTTTACTGGCTCTGATCAGAGAAAACAAGCAAATGCTGCCTTCCTTGTTGGATGCTACATG GTTATATATTTGGGGAGAACCCCAGAAGAAGCATATAGAATATTAATCTTTGGAGAGACATCCTATATTC CTTTCAGAGATGCTGCCTATGGAAGTTGCAATTTCTACATTACACTTCTTGACTGTTTTCATGCAGTAAA GAAGGCAATGCAGTATGGCTTCCTTAATTTCAACTCATTTAACCTTGATGAATATGAACACTATGAAAAA GCAGAAAATGGAGATTTAAATTGGATAATACCAGACCGATTTATTGCCTTCTGTGGACCTCATTCAAGAG CCAGACTTGAAAGTGGTTACCACCAACATTCTCCTGAGACTTATATTCAATATTTTAAGAATCACAATGT TACTACCATTATTCGTCTGAATAAAAGGATGTATGATGCCAAACGCTTTACGGATGCTGGCTTCGATCAC CATGATCTTTTCTTTGCGGATGGCAGCACCCCTACTGATGCCATTGTCAAAGAATTCCTAGATATCTGTG AAAATGCTGAGGGTGCCATTGCAGTACATTGCAAAGCTGGCCTTGGTCGCACGGGCACTCTGATAGCCTG CTACATCATGAAGCATTACAGGATGACAGCAGCCGAGACCATTGCGTGGGTCAGGATCTGCAGACCTGGC TCGGTGATTGGGCCTCAGCAGCAGTTTTTGGTGATGAAGCAAACCAACCTCTGGCTGGAAGGGGACTATT TTCGTCAGAAGTTAAAGGGGCAGGAGAATGGACAACACAGAGCAGCCTTCTCCAAACTTCTCTCTGGCGT TGATGACATTTCCATAAATGGGGTCGAGAATCAAGATCAGCAAGAACCCGAACCGTACAGTGATGATGAC GAAATCAATGGAGTGACACAAGGTGATAGACTTCGGGCCTTGAAAAGCAGAAGACAATCCAAAACAAACG CTATTCCTCTCACTCTCTCCATTTCAAGGACTAAAACAGTCTTGCGTTAA >OriGene 5' read for NM_003671 unedited
GCCATGGTCGTGGCCCCCTGACGGGCCGCGGCCGCCTCCATGAAGCGGAAAAGCGAGCGG CGGTCGAGCTGGGCCGCCGCGCCCCCCTGCTCGCGGCGCTGCTCGTCGACCTCGCCGGGT GTGAAGAAGATCCGCAGCTCCACGCAGCAAGACCCGCGCCGCCGGGACCCCCAGGACGAC GTGTACCTGGACATCACCGATCGCCTTTGTTTTGCCATTCTCTACAGCAGACCAAAGAGT GCATCAAATGTACATTATTTCAGCATAGATAATGAACTTGAATATGAGAACTTCTACGCA GATTTTGGACCCCTCAATCTGGCAATGGGTTACAGATATTGGTGCANGATCAATCAAGAA TTAAAGTCCATTACAATGTTAAGGAAGAAAATCGTTCATTTTACTGGCTCTGATCAGAGA AAACAAGCAAATGCTGCCTTTCCTTGTTGGATGCTCCATGGGTATATATTTTGGGGGGAA CCCCCTGAAGAAGCCTCTTCAATATTAACTCTTTGGAGAGACCTTCCTTATTTCCTTTCC AGAGATGCCTCCCTTTGGTATGTTGCTTTTCTCCTCTTACACTTCTTTGCTGTCTCTCTT GCCCCCCACCAGGGCCTGCCTCCTTGGGTTTTCTGTTTTTCTGTTCTCTTTTTTCTCTCC TTTCTCTTTTCTTCCTTGTCGCTCTTTCCTCACCTTGCTCTTCTCTTCTGTGGCTCGTCT CCTCTCCGCCCTGCCTCTGCCTCTGTACCNGTCTCCTCGCCTCCCCCCTCCTCCGACTTC GTTCTCTCCTTCCTCGCCTCGCCCTCTTCTCCTCTTCCTCCCCGATCTTACTTATTATCG CTTTTCCTCGCTCTTCCACTTCTAATATAACTATAA >OriGene 3' genomic read for NM_003671 unedited
TTACATGATTGTAATTATACAACTTTCCACTATTCGATATTTTGTATAAAACCAGTTACA ACCCACAAGATTTTCAAATGTGACAATATGTATCAAACTACATACATATGCAAAGTTTAC ACGCCATTAGGAAGCTTTATCTTAAAAATACCTTCAGGAAAATAAACATTCATTCAACCA GTTCTCTTGGCTTTAAAAAATATGATTAGAAATATACGCTGTAAAAAAAAATCAAGACAA TGATTGATCAATACTGATCAGTTACATTTTAAAACTTTTAATAATCTGTACATGAGTGCA GGTTANATAAAAGTCTGCACATTATTAATAGAAAACTGCAGTACTATCATCGGATGAACA TGTCTGCTGACAACCCTACCAGGTAATTCAGGAGGTAATTTCCTCCCCAGTTAGGTGACA CGTCCAGTGTTTAACCACATCTGGCGTCCTGCGCCCCTTCCGCCTTTCCCACCCCGTTAC CGTGTGTCATGCGTCGCCTCCCCTCCCCCATTGACGCGCTCTGCTTCTCATGCATCCTCT GATTCCTGACCCCACTCCGCCCCGCGCTTCTCCCCTCCTTGCCTATCTCCTTCTTCCTCC TCGGCCCTACGCCTTCCCTTCTCCCTCAGCCCCCCCCGCCTGCTCTGTTTTTTTTCCCCC CGTGTACCATGCTCCTCCCCCCCCGCCTCCCCCCCTCACATATCCGCCTCTTCTCATCCC CCTTTCCTTTCCCTCCCCTCGCCCTCCACTTCCCCCCCCCCCCCCCCTTCTCCCCTCCCC CCCCCCCCGCGCCACTCTTTTCGCTCGCGCTCACGCCCCCGCCCCTCCCTCTCCGCGTTC TTCGCCACGTTTCTTCTCCTTTCCCCCCCCTCCGCTCCTTATTCTTCTTTCCTTTTTTTT CCCTTTCTTTTTCTTTTCCCCATTCCCGTCCCTTTTTCTCCTCTCCCCCCTCCGGGCCGG TTCTCCCTCTTCTTCTCC |
Restriction Sites | NotI-NotI |
ACCN | NM_003671 |
Insert Size | 4700 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003671.2, NP_003662.1 |
RefSeq Size | 5472 bp |
RefSeq ORF | 5472 bp |
Locus ID | 8555 |
UniProt ID | O60729 |
Domains | Y_phosphatase, DSPc, PTPc_motif |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | Cell cycle |
Gene Summary | The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) lacks an alternate in-frame exon, compared to variant 2, resulting in a shorter protein (isoform 1) that lacks an internal segment in the C-terminal region, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
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RC221121 | CDC14B (Myc-DDK-tagged)-Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 1 |
CNY 4,090.00 |
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RC221121L3 | Lenti-ORF clone of CDC14B (Myc-DDK-tagged)-Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 1 |
CNY 5,990.00 |
|
RC221121L4 | Lenti-ORF clone of CDC14B (mGFP-tagged)-Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 1 |
CNY 5,990.00 |
|
RG221121 | CDC14B (tGFP-tagged) - Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 1 |
CNY 4,470.00 |