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PPP1A (PPP1CA) (NM_001008709) Human Untagged Clone
CAT＃: SC127981
PPP1CA (untagged)-Human protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 3
CNY 3,656.00
CNY 5,510.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PP-1A; PP1A; PP1alpha; PPP1A |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001008709, the custom clone sequence may differ by one or more nucleotides
ATGTCCGACAGCGAGAAGCTCAACCTGGACTCGATCATCGGGCGCCTGCTGGAAGGGTCCAGGGTCCTGA CACCCCATTGCGCCCCAGTGCAGGGCTCGCGGCCTGGCAAGAATGTACAGCTGACAGAGAACGAGATCCG CGGTCTGTGCCTGAAATCCCGGGAGATTTTTCTGAGCCAGCCCATTCTTCTGGAGCTGGAGGCACCCCTC AAGATCTGCGGTGACATACACGGCCAGTACTACGACCTTCTGCGACTATTTGAGTATGGCGGTTTCCCTC CCGAGAGCAACTACCTCTTTCTGGGGGACTATGTGGACAGGGGCAAGCAGTCCTTGGAGACCATCTGCCT GCTGCTGGCCTATAAGATCAAGTACCCCGAGAACTTCTTCCTGCTCCGTGGGAACCACGAGTGTGCCAGC ATCAACCGCATCTATGGTTTCTACGATGAGTGCAAGAGACGCTACAACATCAAACTGTGGAAAACCTTCA CTGACTGCTTCAACTGCCTGCCCATCGCGGCCATAGTGGACGAAAAGATCTTCTGCTGCCACGGAGGCCT GTCCCCGGACCTGCAGTCTATGGAGCAGATTCGGCGGATCATGCGGCCCACAGATGTGCCTGACCAGGGC CTGCTGTGTGACCTGCTGTGGTCTGACCCTGACAAGGACGTGCAGGGCTGGGGCGAGAACGACCGTGGCG TCTCTTTTACCTTTGGAGCCGAGGTGGTGGCCAAGTTCCTCCACAAGCACGACTTGGACCTCATCTGCCG AGCACACCAGGTGGTAGAAGACGGCTACGAGTTCTTTGCCAAGCGGCAGCTGGTGACACTTTTCTCAGCT CCCAACTACTGTGGCGAGTTTGACAATGCTGGCGCCATGATGAGTGTGGACGAGACCCTCATGTGCTCTT TCCAGATCCTCAAGCCCGCCGACAAGAACAAGGGGAAGTACGGGCAGTTCAGTGGCCTGAACCCTGGAGG CCGACCCATCACCCCACCCCGCAATTCCGCCAAAGCCAAGAAATAG >OriGene 5' read for NM_001008709 unedited
AACCACGGGGACNNCCACCCCACCACGCCGGTTCAGATTTGTATACGACTCATATAGGCG GCCGCGAAATCGGCACCAGGTCCGACAGCGAGNAACTCAACCTGGACTCGATCATCGGGC GCCTGCTGGAAGGGTCCAGGGTCCTGACACCCCATTGCGCCCCAGTGCAGGGCTCGCGGC CTGGCAAGAATGTACAGCTGACAGAGAACGAGATCCGCGGTCTGTGCCTGAAATCCCGGG AGATTTTTCTGAGCCAGCCCATTCTTCTGGAGCTGGAGGCACCCCTCAAGATCTGCGGTG ACATACACGGCCAGTACTACGACCTTCTGCGACTATTTGAGTATGGCGGTTTCCCTCCCG AGAGCAACTACCTCTTTCTGGGGGACTATGTGGACAGGGGCAAGCAGTCCTTGGAGACCA TCTGCCTGCTGCTGGCCTATAAGATCAAGTACCCCGAGAACTTCTTCCTGCTCCGTGGGA ACCACGAGTGTGCCAGCATCAACCGCATCTATGGTTTCTACGATGAGTGCAAGAGACGCT ACAACATCAAACTGTGGAAAACCTTCACTGACTGCTTCAACTGCCTGCCCATCGCGGCCA TAGTGGACGAAAAGATCTTCTGCTGCCACGGAGGCCTGTCCCCGGACCTGCAGTCTATGG AGCAGATTCGGCGGATCATGCGGCCCACAGATGTGCCTGACCAGNGCCTGCTGTGTGACC TGCTGTGGTCTGACCCCTGACAAGACGTGCANGGCTGGGGCGAGAACGACCGTGCGTCTC TTTTACCTTTGGAGCCGAGTGGTGGCCAAGTCCTCCACAGCACGACTTGGACCTCATCTG CCGACACACCAGTGGTAGAAGACGCCTATGAGGTCTTTGCCAAGCGCAGCTGGTGACACT TTCTCAGCTCCAACTACTGTGNCGAGTTTGACAATGCTGGCGCCATGATGATGTGGAC >OriGene 3' read for NM_001008709 unedited
GAGCACTGGNGGNAGGGGTCACAGGGCATGCCACCCGGGTACTGTTCAGGAAACAGCTAT GACCGCGGCCGCAATCTAGAGCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCGCTTTGCCCTTTATAA AAAAAACCAAAGGGGTGGCCCCAAAACCCGGGGGCCCCCCCGGAGGCCAAACCGGGGGGG AAACCCAAAACCGGGCCGGCCGTTGCCCGGACCTGCACCCCCGGCCCCAGGATCCTGGTA AAAGCCCCCGGAGGGGCAGGCAAAAAACACCCCGGGGGGACGGGCCCCCCCTTTTGTTTC TTTAAAAAAAAAAAAAAAAAATCCCCCGGGGCCCCTTGTCCCCCGGAACAGGGGGCCCGG AGGGGCCGGGGGACCCCCCCCCCAACAGGGGAGCAGGATTTTGGAACAAAAAACCCATCA TCGGGGGCACAGGGGGGGGGGGGGGGGCAATTTTTGGGCTTTGGCGAAATTGGGGGGGGG GGGGAGGGGCCGCCCCCCAGGGTTCAGGCCAATGAACGGCCCGTACTTCCCCTTGTTTTT GTCGGGGGGCTTGAGGATCTGAAAAAACACATGAGGGTCTCCCCCACACCCATTAGGGGC CCCGCTTTGTCAAACCCCCCACAATATTGGGGACCTGAAAAAATGCCCCCACCTCCCGTT TGGAAAAAAACTAATAGCCGTCTTCAACCCACTGGGGGGCCCCGGAAAAAAGGGCCAAAT CCGCCTTGGGGAAGAACTGGCCCCCCCCCCGGCCCCAAGGTAAAAAAACCCCCCGGTTGT TCTTCCCCACACCGGAGGTTCCTGGGAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001008709 |
| Insert Size | 1500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001008709.1, NP_001008709.1 |
| RefSeq Size | 1521 bp |
| RefSeq ORF | 1026 bp |
| Locus ID | 5499 |
| UniProt ID | P62136 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Focal adhesion, Insulin signaling pathway, Long-term potentiation, Oocyte meiosis, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
| Gene Summary | The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). This broadly expressed gene encodes the alpha subunit of the PP1 complex that associates with over 200 regulatory proteins to form holoenzymes which dephosphorylate their biological targets with high specificity. PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies suggest that PP1 is an important regulator of cardiac function and that PP1 deregulation is implicated in diabetes and multiple types of cancer. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2020] Transcript Variant: This variant (3, also called PP-1 alpha 2) uses an alternate in-frame splice site at the 5' end of an exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is longer compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212268 | PPP1CA (Myc-DDK-tagged)-Human protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 3 |
CNY 3,656.00 |
|
| RC212268L3 | Lenti ORF clone of Human protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212268L4 | Lenti ORF clone of Human protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG212268 | PPP1CA (tGFP-tagged) - Human protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 3 |
CNY 5,256.00 |
