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Cyclin A1 (CCNA1) (NM_003914) Human Untagged Clone
CAT＃: SC127871
CCNA1 (untagged)-Human cyclin A1 (CCNA1), transcript variant 1
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CT146 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127871 sequence for NM_003914 edited (data generated by NextGen Sequencing)
ATGGAGACCGGCTTTCCCGCAATCATGTACCCTGGATCTTTTATTGGGGGCTGGGGAGAA GAGTATCTCAGCTGGGAAGGACCGGGGCTCCCAGATTTCGTCTTCCAGCAGCAGCCCGTG GAGTCTGAAGCAATGCACTGCAGCAACCCCAAGAGTGGAGTTGTGCTGGCTACAGTGGCC CGAGGTCCCGATGCTTGTCAGATACTCACCAGAGCCCCGCTGGGCCAGGATCCCCCGCAG AGGACAGTGCTAGGGCTGCTAACTGCAAATGGGCAGTACAGGAGGACCTGTGGCCAGGGG ATCACAAGAATCAGGTGTTATTCTGGATCAGAAAATGCCTTCCCTCCAGCTGGAAAGAAA GCACTCCCTGACTGTGGGGTCCAAGAGCCCCCCAAGCAAGGGTTTGACATCTACATGGAT GAACTAGAGCAGGGGGACAGAGACAGCTGCTCGGTCAGAGAGGGGATGGCATTTGAGGAT GTGTATGAAGTAGACACCGGCACACTCAAGTCAGACCTGCACTTCCTGCTGGATTTCAAC ACAGTTTCCCCTATGCTGGTAGATTCATCTCTCCTCTCCCAGTCTGAAGATATATCCAGT CTTGGCACAGATGTGATAAATGTGACTGAATATGCTGAAGAAATTTATCAGTACCTTAGG GAAGCTGAAATAAGGCACAGACCCAAAGCACACTACATGAAGAAGCAGCCAGACATCACG GAAGGCATGCGCACGATTCTGGTGGACTGGCTGGTGGAGGTTGGGGAAGAATATAAACTT CGAGCAGAGACCCTGTATCTGGCTGTCAACTTCCTGGACAGGTTCCTTTCATGTATGTCT GTTCTGAGAGGGAAACTGCAGCTCGTAGGAACAGCAGCTATGCTTTTGGCTTCGAAATAT GAAGAGATATATCCTCCTGAAGTAGACGAGTTTGTCTATATCACCGATGATACATACACA AAACGACAACTGTTAAAAATGGAACACTTGCTTCTGAAAGTTCTAGCTTTTGATCTGACA GTACCAACCACCAACCAGTTTCTCCTTCAGTACTTGAGGCGACAAGGAGTGTGCGTCAGG ACTGAGAACCTGGCTAAGTACGTAGCAGAGCTGAGTCTACTTGAAGCAGATCCATTCTTG AAATATCTTCCTTCACTGATAGCTGCAGCAGCTTTTTGCCTGGCAAACTATACTGTGAAC AAGCACTTTTGGCCAGAAACCCTTGCTGCATTTACAGGGTATTCATTAAGTGAAATTGTG CCTTGCCTGAGTGAGCTTCATAAAGCGTACCTTGATATACCCCATCGACCTCAGCAAGCA ATTAGGGAGAAGTACAAGGCTTCAAAGTACCTGTGTGTGTCCCTCATGGAGCCACCTGCA GTTCTTCTTCTACAATAA Clone variation with respect to NM_003914.3 >OriGene 5' read for NM_003914 unedited
GGCGGGTCAAATTTGTATACGACTCATATAGGGCGGCCGCGATTCCCGGGATGACGGGAA GAGCGGGGCCCGCTTTGGGGTCCAGGCAGGTTTTGGGGCCTCCTGTCTGGTGGGAGGAGG CCGCAGCGCAGCACCCTGCTCGTCACTTGGGATGGAGACCGGCTTTCCCGCAATCATGTA CCCTGGATCTTTTATTGGGGGCTGGGGAGAAGAGTATCTCAGCTGGGAAGGACCGGGGCT CCCAGATTTCGTCTTCCAGCAGCCCGTGGAGTCTGAAGCAATGCACTGCAGCAACCCCAA GAGTGGAGTTGTGCTGGCTACAGTGGCCCGAGGTCCCGATGCTTGTCAGATACTCACCAG AGCCCCGCTGGGCCAGGATCCCCCGCAGAGGACAGTGCTAGGGCTGCTAACTGCAAATGG GCAGTACAGGAGGACCTGTGGCCAGGGGATCACAAGAATCAGGTGTTATTCTGGATCAGA AAATGCCTTCCCTCCAGCTGGAAAGAAAGCACTCCCTGACTGTGGGGTCCAAGAGCCCCC CAAGCAAGGGTTTGACATCTACATGGATGAACTAGAGCAGGGGGACAGAGACAGCTGCTC GGTCAGAGAGGGGATGGCATTTGAGGATGTGTATGAAGTAGACACCGGCACACTCAAGTC AGACCTGCACTTCCTGCTGGATTTCAACACAGTTTCCCCTATGCTGGTAGATTCATCTCT CCTCTCCCAGTCTGAAGATATATCCAGTCTTGGCACAGATGTGATAAATGTGACTGAATA TGCTGAAGAATTTATCAGTACCTTNAGGAAGCTNGAATAGNGCACAGACCCAAAGCACAC TACATGAAGAAGCAGCCAGACATCACGGAAGGCATGCGCACGATTTCTGGTGACT |
| Restriction Sites | Please inquire |
| ACCN | NM_003914 |
| Insert Size | 1800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003914.2, NP_003905.1 |
| RefSeq Size | 1743 bp |
| RefSeq ORF | 1398 bp |
| Locus ID | 8900 |
| UniProt ID | P78396 |
| Domains | cyclin_C, CYCLIN, cyclin |
| Protein Families | Druggable Genome |
| Protein Pathways | Acute myeloid leukemia, Cell cycle, Pathways in cancer, Progesterone-mediated oocyte maturation |
| Gene Summary | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). |
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Resources
Other Versions
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| RC206615L2 | Lenti ORF clone of Human cyclin A1 (CCNA1), transcript variant 1, mGFP tagged |
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| RC206615L3 | Lenti ORF clone of Human cyclin A1 (CCNA1), transcript variant 1, Myc-DDK-tagged |
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| RC206615L4 | Lenti ORF clone of Human cyclin A1 (CCNA1), transcript variant 1, mGFP tagged |
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| RG206615 | CCNA1 (tGFP-tagged) - Human cyclin A1 (CCNA1), transcript variant 1 |
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