United States
Germany
Japan
United Kingdom
China
Topoisomerase II alpha (TOP2A) (NM_001067) Human Untagged Clone
CAT＃: SC127832
TOP2A (untagged)-Human topoisomerase (DNA) II alpha 170kDa (TOP2A)
CNY 15,720.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | TOP2; TOP2alpha; TOPIIA; TP2A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC127832 representing NM_001067
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTGTCACCATTGCAGCCTGTAAATGAAAATATGCAAGTCAACAAAATAAAGAAAAATGAAGATG CTAAGAAAAGACTGTCTGTTGAAAGAATCTATCAAAAGAAAACACAATTGGAACATATTTTGCTCCGCCC AGACACCTACATTGGTTCTGTGGAATTAGTGACCCAGCAAATGTGGGTTTACGATGAAGATGTTGGCATT AACTATAGGGAAGTCACTTTTGTTCCTGGTTTGTACAAAATCTTTGATGAGATTCTAGTTAATGCTGCGG ACAACAAACAAAGGGACCCAAAAATGTCTTGTATTAGAGTCACAATTGATCCGGAAAACAATTTAATTAG TATATGGAATAATGGAAAAGGTATTCCTGTTGTTGAACACAAAGTTGAAAAGATGTATGTCCCAGCTCTC ATATTTGGACAGCTCCTAACTTCTAGTAACTATGATGATGATGAAAAGAAAGTGACAGGTGGTCGAAATG GCTATGGAGCCAAATTGTGTAACATATTCAGTACCAAATTTACTGTGGAAACAGCCAGTAGAGAATACAA GAAAATGTTCAAACAGACATGGATGGATAATATGGGAAGAGCTGGTGAGATGGAACTCAAGCCCTTCAAT GGAGAAGATTATACATGTATCACCTTTCAGCCTGATTTGTCTAAGTTTAAAATGCAAAGCCTGGACAAAG ATATTGTTGCACTAATGGTCAGAAGAGCATATGATATTGCTGGATCCACCAAAGATGTCAAAGTCTTTCT TAATGGAAATAAACTGCCAGTAAAAGGATTTCGTAGTTATGTGGACATGTATTTGAAGGACAAGTTGGAT GAAACTGGTAACTCCTTGAAAGTAATACATGAACAAGTAAACCACAGGTGGGAAGTGTGTTTAACTATGA GTGAAAAAGGCTTTCAGCAAATTAGCTTTGTCAACAGCATTGCTACATCCAAGGGTGGCAGACATGTTGA TTATGTAGCTGATCAGATTGTGACTAAACTTGTTGATGTTGTGAAGAAGAAGAACAAGGGTGGTGTTGCA GTAAAAGCACATCAGGTGAAAAATCACATGTGGATTTTTGTAAATGCCTTAATTGAAAACCCAACCTTTG ACTCTCAGACAAAAGAAAACATGACTTTACAACCCAAGAGCTTTGGATCAACATGCCAATTGAGTGAAAA ATTTATCAAAGCTGCCATTGGCTGTGGTATTGTAGAAAGCATACTAAACTGGGTGAAGTTTAAGGCCCAA GTCCAGTTAAACAAGAAGTGTTCAGCTGTAAAACATAATAGAATCAAGGGAATTCCCAAACTCGATGATG CCAATGATGCAGGGGGCCGAAACTCCACTGAGTGTACGCTTATCCTGACTGAGGGAGATTCAGCCAAAAC TTTGGCTGTTTCAGGCCTTGGTGTGGTTGGGAGAGACAAATATGGGGTTTTCCCTCTTAGAGGAAAAATA CTCAATGTTCGAGAAGCTTCTCATAAGCAGATCATGGAAAATGCTGAGATTAACAATATCATCAAGATTG TGGGTCTTCAGTACAAGAAAAACTATGAAGATGAAGATTCATTGAAGACGCTTCGTTATGGGAAGATAAT GATTATGACAGATCAGGACCAAGATGGTTCCCACATCAAAGGCTTGCTGATTAATTTTATCCATCACAAC TGGCCCTCTCTTCTGCGACATCGTTTTCTGGAGGAATTTATCACTCCCATTGTAAAGGTATCTAAAAACA AGCAAGAAATGGCATTTTACAGCCTTCCTGAATTTGAAGAGTGGAAGAGTTCTACTCCAAATCATAAAAA ATGGAAAGTCAAATATTACAAAGGTTTGGGCACCAGCACATCAAAGGAAGCTAAAGAATACTTTGCAGAT ATGAAAAGACATCGTATCCAGTTCAAATATTCTGGTCCTGAAGATGATGCTGCTATCAGCCTGGCCTTTA GCAAAAAACAGATAGATGATCGAAAGGAATGGTTAACTAATTTCATGGAGGATAGAAGACAACGAAAGTT ACTTGGGCTTCCTGAGGATTACTTGTATGGACAAACTACCACATATCTGACATATAATGACTTCATCAAC AAGGAACTTATCTTGTTCTCAAATTCTGATAACGAGAGATCTATCCCTTCTATGGTGGATGGTTTGAAAC CAGGTCAGAGAAAGGTTTTGTTTACTTGCTTCAAACGGAATGACAAGCGAGAAGTAAAGGTTGCCCAATT AGCTGGATCAGTGGCTGAAATGTCTTCTTATCATCATGGTGAGATGTCACTAATGATGACCATTATCAAT TTGGCTCAGAATTTTGTGGGTAGCAATAATCTAAACCTCTTGCAGCCCATTGGTCAGTTTGGTACCAGGC TACATGGTGGCAAGGATTCTGCTAGTCCACGATACATCTTTACAATGCTCAGCTCTTTGGCTCGATTGTT ATTTCCACCAAAAGATGATCACACGTTGAAGTTTTTATATGATGACAACCAGCGTGTTGAGCCTGAATGG TACATTCCTATTATTCCCATGGTGCTGATAAATGGTGCTGAAGGAATCGGTACTGGGTGGTCCTGCAAAA TCCCCAACTTTGATGTGCGTGAAATTGTAAATAACATCAGGCGTTTGATGGATGGAGAAGAACCTTTGCC AATGCTTCCAAGTTACAAGAACTTCAAGGGTACTATTGAAGAACTGGCTCCAAATCAATATGTGATTAGT GGTGAAGTAGCTATTCTTAATTCTACAACCATTGAAATCTCAGAGCTTCCCGTCAGAACATGGACCCAGA CATACAAAGAACAAGTTCTAGAACCCATGTTGAATGGCACCGAGAAGACACCTCCTCTCATAACAGACTA TAGGGAATACCATACAGATACCACTGTGAAATTTGTTGTGAAGATGACTGAAGAAAAACTGGCAGAGGCA GAGAGAGTTGGACTACACAAAGTCTTCAAACTCCAAACTAGTCTCACATGCAACTCTATGGTGCTTTTTG ACCACGTAGGCTGTTTAAAGAAATATGACACGGTGTTGGATATTCTAAGAGACTTTTTTGAACTCAGACT TAAATATTATGGATTAAGAAAAGAATGGCTCCTAGGAATGCTTGGTGCTGAATCTGCTAAACTGAATAAT CAGGCTCGCTTTATCTTAGAGAAAATAGATGGCAAAATAATCATTGAAAATAAGCCTAAGAAAGAATTAA TTAAAGTTCTGATTCAGAGGGGATATGATTCGGATCCTGTGAAGGCCTGGAAAGAAGCCCAGCAAAAGGT TCCAGATGAAGAAGAAAATGAAGAGAGTGACAACGAAAAGGAAACTGAAAAGAGTGACTCCGTAACAGAT TCTGGACCAACCTTCAACTATCTTCTTGATATGCCCCTTTGGTATTTAACCAAGGAAAAGAAAGATGAAC TCTGCAGGCTAAGAAATGAAAAAGAACAAGAGCTGGACACATTAAAAAGAAAGAGTCCATCAGATTTGTG GAAAGAAGACTTGGCTACATTTATTGAAGAATTGGAGGCTGTTGAAGCCAAGGAAAAACAAGATGAACAA GTCGGACTTCCTGGGAAAGGGGGGAAGGCCAAGGGGAAAAAAACACAAATGGCTGAAGTTTTGCCTTCTC CGCGTGGTCAAAGAGTCATTCCACGAATAACCATAGAAATGAAAGCAGAGGCAGAAAAGAAAAATAAAAA GAAAATTAAGAATGAAAATACTGAAGGAAGCCCTCAAGAAGATGGTGTGGAACTAGAAGGCCTAAAACAA AGATTAGAAAAGAAACAGAAAAGAGAACCAGGTACAAAGACAAAGAAACAAACTACATTGGCATTTAAGC CAATCAAAAAAGGAAAGAAGAGAAATCCCTGGTCTGATTCAGAATCAGATAGGAGCAGTGACGAAAGTAA TTTTGATGTCCCTCCACGAGAAACAGAGCCACGGAGAGCAGCAACAAAAACAAAATTCACAATGGATTTG GATTCAGATGAAGATTTCTCAGATTTTGATGAAAAAACTGATGATGAAGATTTTGTCCCATCAGATGCTA GTCCACCTAAGACCAAAACTTCCCCAAAACTTAGTAACAAAGAACTGAAACCACAGAAAAGTGTCGTGTC AGACCTTGAAGCTGATGATGTTAAGGGCAGTGTACCACTGTCTTCAAGCCCTCCTGCTACACATTTCCCA GATGAAACTGAAATTACAAACCCAGTTCCTAAAAAGAATGTGACAGTGAAGAAGACAGCAGCAAAAAGTC AGTCTTCCACCTCCACTACCGGTGCCAAAAAAAGGGCTGCCCCAAAAGGAACTAAAAGGGATCCAGCTTT GAATTCTGGTGTCTCTCAAAAGCCTGATCCTGCCAAAACCAAGAATCGCCGCAAAAGGAAGCCATCCACT TCTGATGATTCTGACTCTAATTTTGAGAAAATTGTTTCGAAAGCAGTCACAAGCAAGAAATCCAAGGGGG AGAGTGATGACTTCCATATGGACTTTGACTCAGCTGTGGCTCCTCGGGCAAAATCTGTACGGGCAAAGAA ACCTATAAAGTACCTGGAAGAGTCAGATGAAGATGATCTGTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001067 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001067.2, NP_001058.2 |
| RefSeq Size | 5698 bp |
| RefSeq ORF | 4596 bp |
| Locus ID | 7153 |
| UniProt ID | P11388 |
| Domains | DNA_gyraseB, DNA_topoisoIV, HATPase_c |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TOP2A Promotes Cell Migration, Invasion and Epithelial–Mesenchymal Transition in Cervical Cancer via Activating the PI3K/AKT Signaling
,Wang, B;Shen, Y;Zou, Y;Qi, Z;Huang, G;,
Cancer Management and Research
,PubMed ID 32547216
[TOP2A]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221568 | TOP2A (Myc-DDK-tagged)-Human topoisomerase (DNA) II alpha 170kDa (TOP2A) |
CNY 15,712.00 |
|
| RC221568L1 | Lenti-ORF clone of TOP2A (Myc-DDK-tagged)-Human topoisomerase (DNA) II alpha 170kDa (TOP2A) |
CNY 18,112.00 |
|
| RC221568L2 | Lenti-ORF clone of TOP2A (mGFP-tagged)-Human topoisomerase (DNA) II alpha 170kDa (TOP2A) |
CNY 17,200.00 |
|
| RC221568L3 | Lenti-ORF clone of TOP2A (Myc-DDK-tagged)-Human topoisomerase (DNA) II alpha 170kDa (TOP2A) |
CNY 17,200.00 |
|
| RC221568L4 | Lenti-ORF clone of TOP2A (mGFP-tagged)-Human topoisomerase (DNA) II alpha 170kDa (TOP2A) |
CNY 18,112.00 |
|
| RG221568 | TOP2A (tGFP-tagged) - Human topoisomerase (DNA) II alpha 170kDa (TOP2A) |
CNY 17,312.00 |
其它Topoisomerase II alpha产品
