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MPRIP (NM_015134) Human Untagged Clone
CAT＃: SC127823
MPRIP (untagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 1
CNY 10,848.00
货期*
现货
规格
| Cited in 1 publication. |
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | M-RIP; MRIP; p116Rip; RHOIP3; RIP3 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127823 sequence for NM_015134 edited (data generated by NextGen Sequencing)
ATGTCGGCAGCCAAGGAGAACCCGTGCAGGAAATTCCAGGCCAACATCTTCAACAAGAGC AAGTGTCAGAACTGCTTCAAGCCCCGCGAGTCGCATCTGCTCAACGACGAGGACCTGACG CAGGCAAAACCCATTTATGGCGGTTGGCTGCTCCTGGCTCCAGATGGGACCGACTTTGAC AACCCAGTGCACCGGTCTCGGAAATGGCAGCGACGGTTCTTCATCCTTTACGAGCACGGC CTCTTGCGCTACGCCCTGGATGAGATGCCCACGACCCTTCCTCAGGGCACCATCAACATG AACCAGTGCACAGATGTGGTGGATGGGGAGGGCCGCACGGGCCAGAAGTTCTCCCTGTGT ATTCTGACGCCTGAGAAGGAGCATTTCATCCGGGCGGAGACCAAGGAGATCGTCAGTGGG TGGCTGGAGATGCTCATGGTCTATCCCCGGACCAACAAGCAGAATCAGAAGAAGAAACGG AAAGTGGAGCCCCCCACACCACAGGAGCCTGGGCCTGCCAAGGTGGCTGTTACCAGCAGC AGCAGCAGCAGCAGCAGCAGCAGCAGCATCCCCAGTGCTGAGAAAGTCCCCACCACCAAG TCCACACTCTGGCAGGAAGAAATGAGGACCAAGGACCAGCCAGATGGCAGCAGCCTGAGT CCAGCTCAGAGTCCCAGCCAGAGCCAGCCTCCTGCTGCCAGCTCCCTGCGGGAACCTGGG CTAGAGAGCAAAGAAGAGGAGAGCGCCATGAGTAGCGACCGCATGGACTGTGGCCGCAAA GTCCGGGTGGAGAGCGGCTACTTCTCTCTGGAGAAGACCAAACAGGACTTGAAGGCTGAA GAACAGCAGCTGCCCCCGCCGCTCTCCCCTCCCAGCCCCAGCACCCCCAACCACAGGAGG TCCCAGGTGATTGAAAAGTTTGAGGCCTTGGACATTGAGAAGGCAGAGCACATGGAGACC AATGCAGTGGGGCCCTCACAATCCAGCGACACACGCCAGGGCCGCAGCGAGAAGAGGGCG TTCCCTAGGAAGCGGGACTTCACCAATGAAGCCCCCCCAGCTCCTCTCCCAGACGCCTCG GCTTCCCCCCTGTCTCCACACCGAAGAGCCAAGTCACTGGACAGGAGGTCCACGGAGCCC TCCGTGACGCCCGACCTGCTGAATTTCAAGAAAGGCTGGCTGACTAAGCAGTATGAGGAC GGCCAGTGGAAGAAACACTGGTTTGTCCTCGCCGATCAAAGCCTGAGATACTACAGGGAT TCAGTGGCTGAGGAGGCAGCCGACTTGGATGGAGAAATTGACTTGTCCGCATGTTACGAT GTCACAGAGTATCCAGTTCAGAGAAACTATGGCTTCCAGATACATACAAAGGAGGGCGAG TTTACCCTGTCGGCCATGACATCTGGGATTCGGCGGAACTGGATCCAGACCATCATGAAG CACGTGCACCCGACCACTGCCCCGGATGTGACCAGCTCGTTGCCAGAGGAAAAAAACAAG AGCAGCTGCTCTTTTGAGACCTGCCCGAGGCCTACTGAGAAGCAAGAGGCAGAGCTGGGG GAGCCGGACCCTGAGCAGAAGAGGAGCCGCGCACGGGAGCGGAGGCGAGAGGGCCGCTCC AAGACCTTTGACTGGGCTGAGTTCCGTCCCATCCAGCAGGCCCTGGCTCAGGAGCGGGTG GGCGGCGTGGGGCCTGCTGACACCCACGAGCCCCTGCGCCCTGAGGCGGAGCCTGGGGAG CTGGAGCGGGAGCGTGCACGGAGGCGGGAGGAGCGCCGCAAGCGCTTCGGGATGCTCGAC GCCACAGACGGGCCAGGCACTGAGGATGCAGCCCTGCGCATGGAGGTGGACCGGAGCCCA GGGCTGCCTATGAGCGACCTCAAAACGCATAACGTCCACGTGGAGATTGAGCAGCGGTGG CATCAGGTGGAGACCACACCTCTCCGGGAAGAGAAGCAGGTGCCCATCGCGCCCGTCCAC CTGTCTTCTGAAGATGGGGGTGACCGGCTCTCCACACACGAGCTGACCTCTCTGCTCGAG AAGGAGCTGGAGCAGAGCCAGAAGGAGGCCTCAGACCTTCTGGAGCAGAACCGGCTCCTG CAGGACCAGCTGAGGGTGGCCCTGGGCCGGGAGCAGAGCGCCCGTGAGGGCTACGTGCTG CAGGCCACGTGCGAGCGAGGGTTTGCAGCAATGGAAGAAACGCACCAGAAGAAGATTGAA GATCTCCAGAGGCAGCACCAGCGGGAGCTAGAGAAACTTCGAGAAGAGAAAGACCGCCTC CTAGCCGAGGAGACAGCGGCCACCATCTCAGCCATCGAAGCCATGAAGAACGCCCACCGG GAGGAAATGGAGCGGGAGCTGGAGAAGAGCCAGCGGTCCCAGATCAGCAGCGTCAACTCG GATGTTGAGGCCCTGCGGCGCCAGTACCTGGAGGAGCTGCAGTCGGTGCAGCGGGAACTG GAGGTCCTCTCGGAGCAGTACTCGCAGAAGTGCCTGGAGAATGCCCATCTGGCCCAGGCG CTGGAGGCCGAGCGGCAGGCCCTGCGGCAGTGCCAGCGTGAGAACCAGGAGCTCAATGCC CACAACCAGGAGCTGAACAACCGCCTGGCTGCAGAGATCACACGGTTGCGGACGCTGCTG ACTGGGGACGGCGGTGGGGAGGCCACTGGGTCACCCCTTGCACAGGGCAAGGATGCCTAT GAACTAGAGGTCTTATTGCGGGTAAAGGAATCGGAAATACAGTACCTGAAACAGGAGATT AGCTCCCTCAAGGATGAGCTGCAGACGGCACTGCGGGACAAGAAGTACGCAAGTGACAAG TACAAAGACATCTACACAGAGCTCAGCATCGCGAAGGCTAAGGCTGACTGTGACATCAGC AGGTTGAAGGAGCAGCTCAAGGCTGCAACGGAAGCACTGGGGGAGAAGTCCCCTGACAGT GCCACGGTGTCCGGATATGATATAATGAAATCTAAAAGCAACCCTGACTTCTTGAAGAAA GACAGATCCTGTGTCACCCGGCAACTCAGAAACATCAGGTCCAAGAGTCTGAAGGAAGGC CTGACGGTGCAAGAACGGTTGAAGCTCTTTGAATCCAGGGACTTGAAGAAAGACTAG Clone variation with respect to NM_015134.3 980 c=>a;1971 c=>g >OriGene 5' read for NM_015134 unedited
CCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAG CTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATT CGGCACGAGGCCCTAGCCCGCCGGGAGCGCCAGGCCGGCCAGGCCTGCGCCGCCGCCGCC GCCGCCGTCGCCGCCGCGCCGACCATGTCGGCAGCCAAGGAGAACCCGTGCAGGAAATTC CAGGCCAACATCTTCAACAAGAGCAAGTGTCAGAACTGCTTCAAGCCCCGCGAGTCGCAT CTGCTCAACGACGAGGACCTGACGCAGGCAAAACCCATTTATGGCGGTTGGCTGCTCCTG GCTCCAGATGGGACCGACTTTGACAACCCAGTGCACCGGTCTCGGAAATGGCAGCGACGG TTCTTCATCCTTTACGAGCACGGCCTCTTGCGCTACGCCCTGGATGAGATGCCCACGACC CTTCCTCAGGGCACCATCAACATGAACCAGTGCACAGATGTGGTGGATGGGGAGGGCCGC ACGGGCCAGAAGTTCTCCCTGTGTATTCTGACGCCTGAGAAGGAGCATTTCATCCGGGCG GAGACCAAGGAGATCGTCAGTGGGTGGCTGGAGATGCTCATGGTCTATCCCCGGNACCAA CAGCAGAATCAGAAGAAGAAACGGAAAGTGGAGCCCCCCACACCACAGGAGCCTGGGCCT GCCCAGGTGGCTGNTACCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCCCCAGTGCT GAGAAAGTCCCCCACACCCAGTCCCACACTCTGGCANGNAGAAATGAGGACCAAGGACCA GCCGATGGCAGCAGCCTGAGTCAGCTCAGAGTCCCAGCCAGACCAGCCTNCTGCTGCCAG CTCCCTGCGGGGACCTGGGCTAGAGAGCAAGAAGAGGAAACGCCATGAGTACGACCGCTG GACTGTGCCCCAAGTCCGGTGGAAAAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015134 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015134.2, NP_055949.2 |
| RefSeq Size | 3858 bp |
| RefSeq ORF | 3117 bp |
| Locus ID | 23164 |
| UniProt ID | Q6WCQ1 |
| Protein Families | Druggable Genome |
| Gene Summary | Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A Novel Marker for Purkinje Cells, KIAA0864 Protein. An Analysis Based on a Monoclonal Antibody HFB-16 in Developing Human Cerebellum
,Yasuhiro Nakamura, Munehiko Yamamoto, Eriko Oda, Yonehiro Kanemura, Eri Kodama, Atsuyo Yamamoto, Hideyuki Yamamoto, Kenji Miyado, Hirotaka James Okano, Ryouji Fukagawa, Koichi Higaki, Mami Yamasaki, and Hideyuki Okano,
J. Histochem. Cytochem. 2005 Apr;53(4):423-30.
[MPRIP]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211386 | MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 1 |
CNY 10,360.00 |
|
| RC211386L3 | Lenti-ORF clone of MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 1 |
CNY 12,260.00 |
|
| RC211386L4 | Lenti-ORF clone of MPRIP (mGFP-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 1 |
CNY 12,260.00 |
|
| RG211386 | MPRIP (tGFP-tagged) - Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 1 |
CNY 13,160.00 |
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