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MPRIP (NM_015134) Human Tagged ORF Clone
CAT＃: RC211386
- TrueORF®
MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 1
ORF Plasmid: tGFP
"NM_015134" in other vectors (4)
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CNY 10,360.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | M-RIP; MRIP; p116Rip; RHOIP3; RIP3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211386 representing NM_015134
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCAGCCAAGGAGAACCCGTGCAGGAAATTCCAGGCCAACATCTTCAACAAGAGCAAGTGTCAGA ACTGCTTCAAGCCCCGCGAGTCGCATCTGCTCAACGACGAGGACCTGACGCAGGCAAAACCCATTTATGG CGGTTGGCTGCTCCTGGCTCCAGATGGGACCGACTTTGACAACCCAGTGCACCGGTCTCGGAAATGGCAG CGACGGTTCTTCATCCTTTACGAGCACGGCCTCTTGCGCTACGCCCTGGATGAGATGCCCACGACCCTTC CTCAGGGCACCATCAACATGAACCAGTGCACAGATGTGGTGGATGGGGAGGGCCGCACGGGCCAGAAGTT CTCCCTGTGTATTCTGACGCCTGAGAAGGAGCATTTCATCCGGGCGGAGACCAAGGAGATCGTCAGTGGG TGGCTGGAGATGCTCATGGTCTATCCCCGGACCAACAAGCAGAATCAGAAGAAGAAACGGAAAGTGGAGC CCCCCACACCACAGGAGCCTGGGCCTGCCAAGGTGGCTGTTACCAGCAGCAGCAGCAGCAGCAGCAGCAG CAGCAGCATCCCCAGTGCTGAGAAAGTCCCCACCACCAAGTCCACACTCTGGCAGGAAGAAATGAGGACC AAGGACCAGCCAGATGGCAGCAGCCTGAGTCCAGCTCAGAGTCCCAGCCAGAGCCAGCCTCCTGCTGCCA GCTCCCTGCGGGAACCTGGGCTAGAGAGCAAAGAAGAGGAGAGCGCCATGAGTAGCGACCGCATGGACTG TGGCCGCAAAGTCCGGGTGGAGAGCGGCTACTTCTCTCTGGAGAAGACCAAACAGGACTTGAAGGCTGAA GAACAGCAGCTGCCCCCGCCGCTCTCCCCTCCCAGCCCCAGCACCCCCAACCACAGGAGGTCCCAGGTGA TTGAAAAGTTTGAGGCCTTGGACATTGAGAAGGCAGAGCACATGGAGACCAATGCAGTGGGGCCCTCACC ATCCAGCGACACACGCCAGGGCCGCAGCGAGAAGAGGGCGTTCCCTAGGAAGCGGGACTTCACCAATGAA GCCCCCCCAGCTCCTCTCCCAGACGCCTCGGCTTCCCCCCTGTCTCCACACCGAAGAGCCAAGTCACTGG ACAGGAGGTCCACGGAGCCCTCCGTGACGCCCGACCTGCTGAATTTCAAGAAAGGCTGGCTGACTAAGCA GTATGAGGACGGCCAGTGGAAGAAACACTGGTTTGTCCTCGCCGATCAAAGCCTGAGATACTACAGGGAT TCAGTGGCTGAGGAGGCAGCCGACTTGGATGGAGAAATTGACTTGTCCGCATGTTACGATGTCACAGAGT ATCCAGTTCAGAGAAACTATGGCTTCCAGATACATACAAAGGAGGGCGAGTTTACCCTGTCGGCCATGAC ATCTGGGATTCGGCGGAACTGGATCCAGACCATCATGAAGCACGTGCACCCGACCACTGCCCCGGATGTG ACCAGCTCGTTGCCAGAGGAAAAAAACAAGAGCAGCTGCTCTTTTGAGACCTGCCCGAGGCCTACTGAGA AGCAAGAGGCAGAGCTGGGGGAGCCGGACCCTGAGCAGAAGAGGAGCCGCGCACGGGAGCGGAGGCGAGA GGGCCGCTCCAAGACCTTTGACTGGGCTGAGTTCCGTCCCATCCAGCAGGCCCTGGCTCAGGAGCGGGTG GGCGGCGTGGGGCCTGCTGACACCCACGAGCCCCTGCGCCCTGAGGCGGAGCCTGGGGAGCTGGAGCGGG AGCGTGCACGGAGGCGGGAGGAGCGCCGCAAGCGCTTCGGGATGCTCGACGCCACAGACGGGCCAGGCAC TGAGGATGCAGCCCTGCGCATGGAGGTGGACCGGAGCCCAGGGCTGCCTATGAGCGACCTCAAAACGCAT AACGTCCACGTGGAGATTGAGCAGCGGTGGCATCAGGTGGAGACCACACCTCTCCGGGAAGAGAAGCAGG TGCCCATCGCCCCCGTCCACCTGTCTTCTGAAGATGGGGGTGACCGGCTCTCCACACACGAGCTGACCTC TCTGCTCGAGAAGGAGCTGGAGCAGAGCCAGAAGGAGGCCTCAGACCTTCTGGAGCAGAACCGGCTCCTG CAGGACCAGCTGAGGGTGGCCCTGGGCCGGGAGCAGAGCGCCCGTGAGGGCTACGTGCTGCAGGCCACGT GCGAGCGAGGGTTTGCAGCAATGGAAGAAACGCACCAGAAGAAGATTGAAGATCTCCAGAGGCAGCACCA GCGGGAGCTAGAGAAACTTCGAGAAGAGAAAGACCGCCTCCTAGCCGAGGAGACAGCGGCCACCATCTCA GCCATCGAAGCCATGAAGAACGCCCACCGGGAGGAAATGGAGCGGGAGCTGGAGAAGAGCCAGCGGTCCC AGATCAGCAGCGTCAACTCGGATGTTGAGGCCCTGCGGCGCCAGTACCTGGAGGAGCTGCAGTCGGTGCA GCGGGAACTGGAGGTCCTCTCGGAGCAGTACTCGCAGAAGTGCCTGGAGAATGCCCATCTGGCCCAGGCG CTGGAGGCCGAGCGGCAGGCCCTGCGGCAGTGCCAGCGTGAGAACCAGGAGCTCAATGCCCACAACCAGG AGCTGAACAACCGCCTGGCTGCAGAGATCACACGGTTGCGGACGCTGCTGACTGGGGACGGCGGTGGGGA GGCCACTGGGTCACCCCTTGCACAGGGCAAGGATGCCTATGAACTAGAGGTCTTATTGCGGGTAAAGGAA TCGGAAATACAGTACCTGAAACAGGAGATTAGCTCCCTCAAGGATGAGCTGCAGACGGCACTGCGGGACA AGAAGTACGCAAGTGACAAGTACAAAGACATCTACACAGAGCTCAGCATCGCGAAGGCTAAGGCTGACTG TGACATCAGCAGGTTGAAGGAGCAGCTCAAGGCTGCAACGGAAGCACTGGGGGAGAAGTCCCCTGACAGT GCCACGGTGTCCGGATATGATATAATGAAATCTAAAAGCAACCCTGACTTCTTGAAGAAAGACAGATCCT GTGTCACCCGGCAACTCAGAAACATCAGGTCCAAGAGTCTGAAGGAAGGCCTGACGGTGCAAGAACGGTT GAAGCTCTTTGAATCCAGGGACTTGAAGAAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211386 representing NM_015134
Red=Cloning site Green=Tags(s) MSAAKENPCRKFQANIFNKSKCQNCFKPRESHLLNDEDLTQAKPIYGGWLLLAPDGTDFDNPVHRSRKWQ RRFFILYEHGLLRYALDEMPTTLPQGTINMNQCTDVVDGEGRTGQKFSLCILTPEKEHFIRAETKEIVSG WLEMLMVYPRTNKQNQKKKRKVEPPTPQEPGPAKVAVTSSSSSSSSSSSIPSAEKVPTTKSTLWQEEMRT KDQPDGSSLSPAQSPSQSQPPAASSLREPGLESKEEESAMSSDRMDCGRKVRVESGYFSLEKTKQDLKAE EQQLPPPLSPPSPSTPNHRRSQVIEKFEALDIEKAEHMETNAVGPSPSSDTRQGRSEKRAFPRKRDFTNE APPAPLPDASASPLSPHRRAKSLDRRSTEPSVTPDLLNFKKGWLTKQYEDGQWKKHWFVLADQSLRYYRD SVAEEAADLDGEIDLSACYDVTEYPVQRNYGFQIHTKEGEFTLSAMTSGIRRNWIQTIMKHVHPTTAPDV TSSLPEEKNKSSCSFETCPRPTEKQEAELGEPDPEQKRSRARERRREGRSKTFDWAEFRPIQQALAQERV GGVGPADTHEPLRPEAEPGELERERARRREERRKRFGMLDATDGPGTEDAALRMEVDRSPGLPMSDLKTH NVHVEIEQRWHQVETTPLREEKQVPIAPVHLSSEDGGDRLSTHELTSLLEKELEQSQKEASDLLEQNRLL QDQLRVALGREQSAREGYVLQATCERGFAAMEETHQKKIEDLQRQHQRELEKLREEKDRLLAEETAATIS AIEAMKNAHREEMERELEKSQRSQISSVNSDVEALRRQYLEELQSVQRELEVLSEQYSQKCLENAHLAQA LEAERQALRQCQRENQELNAHNQELNNRLAAEITRLRTLLTGDGGGEATGSPLAQGKDAYELEVLLRVKE SEIQYLKQEISSLKDELQTALRDKKYASDKYKDIYTELSIAKAKADCDISRLKEQLKAATEALGEKSPDS ATVSGYDIMKSKSNPDFLKKDRSCVTRQLRNIRSKSLKEGLTVQERLKLFESRDLKKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015134 |
| ORF Size | 3114 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015134.4 |
| RefSeq Size | 3858 bp |
| RefSeq ORF | 3117 bp |
| Locus ID | 23164 |
| UniProt ID | Q6WCQ1 |
| Protein Families | Druggable Genome |
| MW | 117.9 kDa |
| Gene Summary | Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211386L3 | Lenti-ORF clone of MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 1 |
CNY 12,260.00 |
|
| RC211386L4 | Lenti-ORF clone of MPRIP (mGFP-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 1 |
CNY 12,260.00 |
|
| RG211386 | MPRIP (tGFP-tagged) - Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 1 |
CNY 13,160.00 |
|
| SC127823 | MPRIP (untagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 1 |
CNY 10,848.00 |
