ATP6V0A2 (AK056800) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	A2; ARCL; ARCL2A; ATP6A2; ATP6N1D; J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK056800, the custom clone sequence may differ by one or more nucleotides ACATTTATTCTGCTGGATTTAAAAATATGTACTTTTTTTTTTTTTGAGATGAAGTCTTGCTGTGTCACGT AGGCTGGAGTGCAATGGCACGATCGTGTCTCATTGCAACTTCCGCCTCCTGGATTCAGGTGATTCTCCTG TCTCAGCCTCCCGAGTAACTGGGATTACAGGCATGCGCCACCGTGACCAGCTAATTTTTTTGTATTTTTG TAGAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTTCTGACCTCAAGTGATCCACCTGCCTC GGCCTCCCTAAGTGCTGGGATTACAGGCGTGAGCCACCATGCCTGGCCAAAATATGCAAATATTTTTTAT CCTGTGCTACCTAGAATTACAAGTAGAGGATTGTTTTTACAATTTTGTCTTGTTTTAGTAATTGGGGACA ATGGGATAAAATGACATACAACAAATGGTTATCCCACTTGTATATTTTTTTAAAGACTCTTATCCTGTTA CCCTTACATAAAACCAGTTATGATAATTGTATGTTTGGAAGATAAATTGTATGCTGAGCCTTAAAACCCA GTGTGACTGTAGAAGATATAATGCGGACATGATTTGAATTTAGTGACAGCTTGGCTGATGTCACTGAACC AGAAGGGTTTATACTGAGTGAAGGAAAGGTAAAAAGCAGTATTTTGTATATTTTTGTAACAAAATATAAA TGAAGATATTTTACCATGAAGAGATTGCACTTATCCTTGAGAACAGTCTAAATATAAATGAAGATATTTT ACCATGAAGAGATTGCACTTATCCTTGAGAACAGTCTAATGATACTTTTTTTACTTTCAGACTGTTTCTA GCTAAAGTTCTGATAAAAGTATTGAGTTAATTTAAACTTTCAAACACTGTTTAGATCAGTTGAAAACTAA TTCTTAATAGCCATTGTTTTATAATCTTTAGTTTAAATTCATCTGTTTGACATTTGGTTTTTTGTGAACT TGAGTCAGTCCTATTGAACATTTTGACCTATATTCTTGCAAATTATGTTAATTTTTCATATTTTAGTTTG ATTTTAGACAATTATTTTCAAAAAGAAACCTGACTTTTTTTCACCTTCTTGACATTGTGAGTGATACACG TTAAAAGGCTATTTGCCCGCATCCTCCTCTATCCTGTTTCGAGGTAGAGAGCTTGGCACTTCTGTTACAA GCTGAGAGTCTGGAGGTGCTGAGTTTAACGTGGCTGTGCAGGTGATTTCAAGGCATGAGCTGAACAGCGT TGACACAGCCGTGACTTACAATTAAACCCTTTCAATTACAGTACAGATTCTGTGTGCTACTTGATGAGAT GTTACCCAAGGCCAGTTTGGACTTACTGCTTTTTGTCCCTCATATTAAATTATCAAATGATGCCGATGAA ATGCTTGAAAATGATTCTGCTCTCAAAGTTATGCTTCCCTAGAAAGGTGTCATGTCGGGCACTTGTGTTG GGTGACTATGACATCCCAGCAGTGCTGGTCAGGTCTCAGAGTTGCTTTCACCTAGAGCTGATGCTTCCAC AAGGGGACATTTGTGTTACTTTGCTCCAGGGGTCTGTCATAAAAACAATGGTAACTACTTCATCCCTAGG AAGCCCAAGACTTCAGGGCACTGTTAGCTGATTGGTAAATAAGGGACACGATATTCACGGGAATTGTTTA CTGCTCCCCACTAGTAATTCTCATGGGGCCACTTTTTTAACTCTTTCTAATTAACACCTCTTCTCAGCAT AGAGCAGAGGATGCAGTCATTTCTCTGTTGAAATCTATGTAGTATTTATGTAGAATGTCATTATATGAAA AGCGAATTCAGAATCCTAACTCTGGGAAAGCGCTCTCTGGAAATGTAGTTTGATAATAGTGTCTTATAGG GGCCACGGGAATTTGTTTCTCTATAAAGCGATGGGCTCCAGTGTCATGTTACCAGAGTTATCGCTCAGTT TTGTTTGTTAAGGCTTTTGCTAGAACCTATTATGTATCCAGTTTTTAAAACATACTTCATTCCCTTGATA GCAGAGATCCAGAGCACAGATGGCAAGTTGTGTGGATGATACTGAATATGTTTATTTTCCTAAAGATAAA TGTCACGACACGACATTTCTCAGTTTTTATTTAGATGTTTATTACCATGTGTTAACAGAATCTTAAAACC CAAGGGATTTCTTCAGTAAACTAGACTTTGATTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK056800 unedited GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATTATTTTCAAAAAGAA ACCTGACTTTTTTTCACCTTCTTGACATTGTGAGTGATACACGTTAAAAGGCTATTTGCC CGCATCCTCCTCTATCCTGTTTCGAGGTAGAGAGCTTGGCACTTCTGTTACAAGCTGAGA GTCTGGAGGTGCTGAGTTTAACGTGGCTGTGCAGCTGATTTCAAGGCATGAGCTGAACAG CGTTGACACAGCCGTGACTTACAATTAAACCCTTTCAATTACAGTACAGATTCTGTGTGC TACTTGATGAGATGTTACCCAAGGCCAGTTTGGACTTACTGCTTTTTGTCCCTCATATTA AATTATCAAATGATGCCGATGAAATGCTTGAAAATGATTCTGCTCTCAAAGTTATGCTTC CCTAGAAAGGTGTCATGTCGGGCACTTGTGTTGGGTGACTATGACATCCCAGCAGTGCTG GTCAGGTCTCAGAGTTGCTTTCACCTAGAGCTGATGCTTCCACAAGGGGACATTTGTGTT ACTTTGCTCCAGGGGTCTGTCATAAAAACAATGGTAACTACTTCATCCCTAGGAAGCCCA AGACTTCAGGGCACTGTTAGCTGATTGGTAAATAAGGGACACGATATTCACGGGAATTGT TTACTGCTCCCCACTAGTAATTCTCATGGGGCCACTNTNTTAACTCTTTCTAATTAACAC CTCTTCTCAGCATAGAGCAGAGGATGCAGTCATTTCTCTGGTGAATCTATGTAGTATTTA TGTAGAATGTNCATATATGAAAAGCGAATCAGAATCCTACTCTGGAAAGCGCTCTCTGGA AAGTAGNTGATATAGTGCTTATAGGGCACGGNAANTTGNTCTCTTAAGCGATGGCTCCAG NGTCATGTACAGAGTTACGTCAGTTTGTT
Restriction Sites	NotI-NotI
ACCN	AK056800
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK056800.1
RefSeq Size	2204 bp
RefSeq ORF	2204 bp
Locus ID	23545
Protein Families	Transmembrane
Protein Pathways	Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
Gene Summary	The protein encoded by this gene is a subunit of the vacuolar ATPase (v-ATPase), an heteromultimeric enzyme that is present in intracellular vesicles and in the plasma membrane of specialized cells, and which is essential for the acidification of diverse cellular components. V-ATPase is comprised of a membrane peripheral V(1) domain for ATP hydrolysis, and an integral membrane V(0) domain for proton translocation. The subunit encoded by this gene is a component of the V(0) domain. Mutations in this gene are a cause of both cutis laxa type II and wrinkly skin syndrome. [provided by RefSeq, Jul 2009]

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