YAP1 (NM_006106) Human Untagged Clone

CAT#: SC127739

YAP1 (untagged)-Human Yes-associated protein 1 (YAP1), transcript variant 2



  "NM_006106" in other vectors (6)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
Mouse Monoclonal YAP1 Antibody (1A12)
    • 100 ul

CNY 5,521.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms COB1; YAP; YAP2; YAP65; YKI
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC127739 sequence for NM_006106 edited (data generated by NextGen Sequencing)
ATGGATCCCGGGCAGCAGCCGCCGCCTCAACCGGCCCCCCAGGGCCAAGGGCAGCCGCCT
TCGCAGCCCCCGCAGGGGCAGGGCCCGCCGTCCGGACCCGGGCAACCGGCACCCGCGGCG
ACCCAGGCGGCGCCGCAGGCACCCCCCGCCGGGCATCAGATCGTGCACGTCCGCGGGGAC
TCGGAGACCGACCTGGAGGCGCTCTTCAACGCCGTCATGAACCCCAAGACGGCCAACGTG
CCCCAGACCGTGCCCATGAGGCTCCGGAAGCTGCCCGACTCCTTCTTCAAGCCGCCGGAG
CCCAAATCCCACTCCCGACAGGCCAGTACTGATGCAGGCACTGCAGGAGCCCTGACTCCA
CAGCATGTTCGAGCTCATTCCTCTCCAGCTTCTCTGCAGTTGGGAGCTGTTTCTCCTGGG
ACACTGACCCCCACTGGAGTAGTCTCTGGCCCAGCAGCTACACCCACAGCTCAGCATCTT
CGACAGTCTTCTTTTGAGATACCTGATGATGTACCTCTGCCAGCAGGTTGGGAGATGGCA
AAGACATCTTCTGGTCAGAGATACTTCTTAAATCACATCGATCAGACAACAACATGGCAG
GACCCCAGGAAGGCCATGCTGTCCCAGATGAACGTCACAGCCCCCACCAGTCCACCAGTG
CAGCAGAATATGATGAACTCGGCTTCAGCCATGAACCAGAGAATCAGTCAGAGTGCTCCA
GTGAAACAGCCACCACCCCTGGCTCCCCAGAGCCCACAGGGAGGCGTCATGGGTGGCAGC
AACTCCAACCAGCAGCAACAGATGCGACTGCAGCAACTGCAGATGGAGAAGGAGAGGCTG
CGGCTGAAACAGCAAGAACTGCTTCGGCAGGAGTTAGCCCTGCGTAGCCAGTTACCAACA
CTGGAGCAGGATGGTGGGACTCAAAATCCAGTGTCTTCTCCCGGGATGTCTCAGGAATTG
AGAACAATGACGACCAATAGCTCAGATCCTTTCCTTAACAGTGGCACCTATCACTCTCGA
GATGAGAGTACAGACAGTGGACTAAGCATGAGCAGCTACAGTGTCCCTCGAACCCCAGAT
GACTTCCTGAACAGTGTGGATGAGATGGATACAGGTGATACTATCAACCAAAGCACCCTG
CCCTCACAGCAGAACCGTTTCCCAGACTACCTTGAAGCCATTCCTGGGACAAATGTGGAC
CTTGGAACACTGGAAGGAGATGGAATGAACATAGAAGGAGAGGAGCTGATGCCAAGTCTG
CAGGAAGCTTTGAGTTCTGACATCCTTAATGACATGGAGTCTGTTTTGGCTGCCACCAAG
CTAGATAAAGAAAGCTTTCTTACATGGTTATAG

Clone variation with respect to NM_006106.4
>OriGene 5' read for NM_006106 unedited
CGCGAATTCCGCACCAGGGGCGCAGGGCGGGGGCGGAGGCGCCGGGGCGGGGGATGCGGG
GCCGCGGCGCAACCCCCCGGCCCTGAGAGCGAGGACAGCGCCGCCCGGCCCGCAGCCGTC
GCCGCTTCTCCACCTCGGCCCGTGGAGCCGGGGCGTCCGGGCGTANCCCTCGCTCGCCTG
GGTCAGGGGGTGCGCGTCGGGGGAGGCAGAAGCCATGGATCCCGGGCAGCAGCCGCCGCC
TCAACCGGCCCCCCAGGGCCAAGGGCAGCCGCCTTCGCAGCCCCCGCAGGGGCAGGGCCC
GCCGTCCGGACCCGGGCAACCGGCACCCGCGGCGACCCAGGCGGCGCCGCAGGCACCCCC
CGCCGGGCATCAGATCGTGCACGTCCGCGGGGACTCGGAGACCGACCTGGAGGCGCTCTT
CAACGCCGTCATGAACCCCAAGACGGCCAACGTGCCCCAGACCGTGCCCATGAGGCTCCG
GAAGCTGCCCGACTCCTTCTTCAAGCCGCCGGAGCCCANATCCCACTCCCGACAGGCCAG
TACTGATGCAGGCACTGCAGGAGCCCTGACTCCACAGCATGTTCGAGCTCATTCCTCTCC
AGCTTCTCTGCAGGTGGGAGCTGTTTCTCCTGGGACACTGACCCCCACTGGAGTAGTCTC
TGGCCCAGCAGCTACACCCACAGCTCAGCATCTTCGACAGTCTTCTTTTGAGAACCTGAT
GATGTACCTCTGCCAGCN
>OriGene 3' read for NM_006106 unedited
AGTAATTCGGGTTNATGAACCACATCAGGGACAAATTGAATACATAAGAAAAAAAGTAAA
TTTTATGTAAGTACCTAACATATGAGCATGCTCTTACATCTAAAACAAAAAATAAAAAGG
TAACATTGGTACTATATATATATATTTGACAAGTGTGCATTAAAGAATTCTCTAATATAA
AACATTTAAAATGTGGAGAATACTTTTTTATTATACAGAAAACAATTGTTATGATAGGCA
CACCCACAATTCTTATAACAACATGCTTGCGAGGATAAAATCCACCTGAGCACTCATTTC
ACAGATGTACCAACGCTAGAAAAGTGTTAAGCACTGAATATTGCCACCCACTTTTGCAAT
GTTTGAGTTTCAACACTGATTGGTATGAATTCTGAATTACACAATTAATTACTGTTATTT
TTCAGTCTTTCTGCCATGTTCCATATAGAAGGCATGTATTTAATATGAATACTTAACACA
GCAACATTATTTGTAGCAAAGTCACTTCCCTGTGTTCATTTTTCCTTTAAAGGCACTACA
TTTAGAAAAGGTAATACTACAAAAAGTGCTTTGGAAAATCTGAAACTCCAAATCAATGTG
CTTCATCCACCAAAAGTGTATCTTAGAAGCCCTGACTGAAAATACCCACATGTAAAAGTG
GTTAATTTTAAAGATTTAAAATTGGGTTATTGGTTAAGCAAGTTTAGAATTCCCGTTAAA
ATTTTATTCCAAGGTTTCGCATGTAACCAGAAACGGTGAAGACCCTGTTCCTATGGCATA
AGACGTTTATCAGGTCTCCAGCAGGGCTTTGTTTCCAGTCAAGAGGGAAAGCACTCTGAG
ACTTCTTCAGGTCAATACGTTGAGGTAATTACGACCAATCCTGGCCAGCGGTACC
Restriction Sites NotI-NotI     
ACCN NM_006106
Insert Size 4700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006106.2, NP_006097.1
RefSeq Size 5128 bp
RefSeq ORF 1365 bp
Locus ID 10413
Domains WW
Protein Families Druggable Genome
Gene Summary This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
Transcript Variant: This variant (2) uses an alternate in-frame splice site and lacks two alternate exons in the coding region, compared to variant 9. The encoded protein (isoform 2) represents the YAP1-1alpha isoform described in Figure 3 of PMID: 22939869. It is shorter, compared to isoform 9. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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