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ALDH7A1 (NM_001182) Human Untagged Clone
CAT＃: SC127737
ALDH7A1 (untagged)-Human aldehyde dehydrogenase 7 family, member A1 (ALDH7A1), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 5,720.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATQ1; EPD; PDE |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001182, the custom clone sequence may differ by one or more nucleotides
ATGTGGCGCCTTCCTCGCGCGCTGTGTGTGCACGCTGCAAAGACCAGCAAGCTCTCTGGACCTTGGAGCA GGCCTGCCGCCTTCATGTCCACTCTCCTCATCAATCAGCCCCAGTATGCGTGGCTGAAAGAGCTGGGGCT CCGCGAGGAAAACGAGGGCGTGTATAATGGAAGCTGGGGAGGCCGGGGAGAGGTTATTACGACCTATTGC CCTGCTAACAACGAGCCAATAGCAAGAGTCCGACAGGCCAGTGTGGCAGACTATGAAGAAACTGTAAAGA AAGCAAGAGAAGCATGGAAAATCTGGGCAGATATTCCTGCTCCAAAACGAGGAGAAATAGTAAGACAGAT TGGCGATGCCTTGCGGGAGAAGATCCAAGTACTAGGAAGCTTGGTGTCTTTGGAGATGGGGAAAATCTTA GTGGAAGGTGTGGGTGAAGTTCAGGAGTATGTGGATATCTGTGACTATGCTGTTGGTTTATCAAGGATGA TTGGAGGACCTATCTTGCCTTCTGAAAGATCTGGCCATGCACTGATTGAGCAGTGGAATCCCGTAGGCCT GGTTGGAATCATCACGGCATTCAATTTCCCTGTGGCAGTGTATGGTTGGAACAACGCCATCGCCATGATC TGTGGAAATGTCTGCCTCTGGAAAGGAGCTCCAACCACTTCCCTCATTAGTGTGGCTGTCACAAAGATAA TAGCCAAGGTTCTGGAGGACAACAAGCTGCCTGGTGCAATTTGTTCCTTGACTTGTGGTGGAGCAGATAT TGGCACAGCAATGGCCAAAGATGAACGAGTGAACCTGCTGTCCTTCACTGGGAGCACTCAGGTGGGAAAA CAGGTGGGCCTGATGGTGCAGGAGAGGTTTGGGAGAAGTCTGTTGGAACTTGGAGGAAACAATGCCATTA TTGCCTTTGAAGATGCAGACCTCAGCTTAGTTGTTCCATCAGCTCTCTTCGCTGCTGTGGGAACAGCTGG CCAGAGGTGTACCACTGCGAGGCGACTGTTTATACATGAAAGCATCCATGATGAGGTTGTAAACAGACTT AAAAAGGCCTATGCACAGATCCGAGTTGGGAACCCATGGGACCCTAATGTTCTCTATGGGCCACTCCACA CCAAGCAGGCAGTGAGCATGTTTCTTGGAGCAGTGGAAGAAGCAAAGAAAGAAGGTGGCACAGTGGTCTA TGGGGGCAAGGTTATGGATCGCCCTGGAAATTATGTAGAACCGACAATTGTGACAGGTCTTGGCCACGAT GCGTCCATTGCACACACAGAGACTTTTGCTCCGATTCTCTATGTCTTTAAATTCAAGAATGAAGAAGAGG TCTTTGCATGGAATAATGAAGTAAAACAGGGACTTTCAAGTAGCATCTTTACCAAAGATCTGGGCAGAAT CTTTCGCTGGCTTGGACCTAAAGGATCAGACTGTGGCATTGTAAATGTCAACATTCCAACAAGTGGGGCT GAGATTGGAGGTGCCTTTGGAGGAGAAAAGCACACTGGTGGTGGCAGGGAGTCTGGCAGTGATGCCTGGA AACAGTACATGAGAAGGTCTACTTGTACTATCAACTACAGTAAAGACCTTCCTCTGGCCCAAGGAATCAA GTTTCAGTAA >OriGene 5' read for NM_001182 unedited
GGTTCACATATGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGGCGCCTTCC TCGCGCGCTGTGTGTGCACGCTGCAAAGACCAGCAAGCTCTCTGGACCTTGGAGCAGGCC TGCCGCCTTCATGTCCACTCTCCTCATCAATCAGCCCCAGTATGCGTGGCTGAAAGAGCT GGGGCTCCGCGAGGAAAACGAGGGCGTGTATAATGGAAGCTGGGGAGGCCGGGGAGAGGT TATTACGACCTATTGCCCTGCTAACAACGAGCCAATAGCAAGAGTCCGACAGGCCAGTGT GGCAGACTATGAAGAAACCGTAAAGAAAGCAAGAGAAGCATGGAAAATCTGGGCAGATAT TCCTGCTCCAAAACGAGGAGAAATAGTAAGACAGATTGGCGATGCCTTGCGGGAGAAGAT CCAAGTACTAGGAAGCTTGGTGTCTTTGGAGATGGGGAAAATCTTAGTGGAAGGTGTGGG TGAAGTTCAGGAGTATGTGGATATCTGTGACTATGCTGTTGGTTTATCAAGGATGATTGG AGGACCTATCTTGCCTTCTGAAAGATCTGGCCATGCACTGATTGAGCAGTGGAATCCCGT AGGCCTGGTTGGAATCATCACGGCATTCAATTTCCCTGTGGCAGTGTATGGTTGGAACAA CGCCATCGCCATGATCTGTGGAAATGTCTGCCTCTGGAAAGGAGCTCCAACCACTTCCCT CATTAGTGTGGCTGTCACANAGATAATAGCCAAGGTTCTGGAGGACAACAAGCTGCCTGG TGCAATTTGTTCCTTGACTTGTGGTGGAGCAGATATGCCACAGNCATGGCCAAAGATGAC GAGTGACCTGCTGTCCTCCACTGGGAGCACTCAGTGGGAAAACAGTGGCCCTGATGGTGC ANGAGAGGTTA >OriGene 3' read for NM_001182 unedited
CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTATGTTGTAACAATTT TATTTTGATTTTTAAAAAAGGAATCTCTTGATTTAATCAGGGCTTTGGGGTCATAGGGGG ATTAGTCACTGTCACAGTCATAATAATGCATTTATTCAGGGAAAACTTTAATCTTCTTTG TCTTCTCCAAAAACAGCTGCTGGAACACCTCAAATTAAGGGATGTTCATCTAAAACACCT TTACTGAAACTTGATTCCTTGGGCCAGAGGAAGGTCTTTACTGTAGTTGATAGTACAAGT AGACCTTCTCATGTACTGTTTCCAGGCATCACTGCCAGACTCCCTGCCACCACCAGTGTG CTTTTCTCCTCCAAAGGCACCTCCAATCTCAGCCCCACTTGTTGGAATGTTGACATTTAC AATGCCACAGTCTGATCCTTTAGGTCCAAGCCAGCGAAAGATTCTGCCCAGATCTTTGGT AAAGATGCTACTTGAAAGTCCCTGTTTTACTTCATTATTCCATGCAAAGACCTCTTCTTC ATTCTTGAATTTAAAGACATAGAGAATCGGAGCAAAAGTCTCTGTGTGTGCAATGGACGC ATCGTGGCCAAGACCTGTCACAATTGTCGGTTCTACATAATTTCCAGGGCGATCCATAAC CTTGCCCCCATAGACCACTGTGCCACCTTCTTTCTTTGCTTCTTCCACTGCTCCAAGAAA CATGCTCACTGCCTGCTTGGTGTGGAGTGGCCCATNAGAGACATTANGGTCCCATGGGTT CCCAACTCGNATCTGTGCATANGCCTTTTTAAGTCTGTTTACAACCTCATCATGGATGCT TTCATGTATAAACAGTCGCCTCGCAGTGGTACACCTCTGGCCAGCTNGTCCACAGCAGCG AANAGACTGATGGACACTAAACTGAGTCTGCATCTCAAGCATAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001182 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001182.2, NP_001173.1 |
| RefSeq Size | 3566 bp |
| RefSeq ORF | 1536 bp |
| Locus ID | 501 |
| UniProt ID | P49419 |
| Domains | aldedh |
| Protein Families | Druggable Genome |
| Protein Pathways | Arginine and proline metabolism, Ascorbate and aldarate metabolism, beta-Alanine metabolism, Butanoate metabolism, Fatty acid metabolism, Glycerolipid metabolism, Glycolysis / Gluconeogenesis, Histidine metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation |
| Gene Summary | The protein encoded by this gene is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. It is also involved in lysine catabolism that is known to occur in the mitochondrial matrix. Recent reports show that this protein is found both in the cytosol and the mitochondria, and the two forms likely arise from the use of alternative translation initiation sites. An additional variant encoding a different isoform has also been found for this gene. Mutations in this gene are associated with pyridoxine-dependent epilepsy. Several related pseudogenes have also been identified. [provided by RefSeq, Jan 2011] Transcript Variant: This variant (1) encodes two isoforms resulting from the use of alternative in-frame translation initiation codons. The longer isoform (1) is derived from an upstream AUG (at nt 193-195), while the shorter isoform (2) is derived from a downstream AUG (at nt 277-279). This RefSeq represents the longer isoform, which resides in the mitochondria (PMIDs: 20207735 and 19885858). Sequence Note: This Refseq, containing three potential in-frame translation initiation codons (all with weak Kozak signals), is annotated with a CDS starting from a downstream start codon (at nt 193-195) based on better conservation, N-terminal consistency with homologous proteins, and the presence of a transit peptide, which is essential for the localization of this isoform in the mitochondria (PMIDs: 20207735 and 19885858), and is consistent with the function of this gene in lysine catabolism (which is known to occur in the mitochondria). The use of an upstream start codon (at nt 112-114) that is present in only a subset of higher mammals, would increase the protein length by 27 aa. A shorter, soluble isoform resulting from the use of another downstream start codon (at nt 277-279) is represented in a separate RefSeq (NM_001201377.1). This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229276 | ALDH7A1 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 7 family, member A1 (ALDH7A1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 6,016.00 |
|
| RC229276L1 | Lenti ORF clone of Human aldehyde dehydrogenase 7 family, member A1 (ALDH7A1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 8,416.00 |
|
| RC229276L3 | Lenti ORF clone of Human aldehyde dehydrogenase 7 family, member A1 (ALDH7A1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RG229276 | ALDH7A1 (tGFP-tagged) - Human aldehyde dehydrogenase 7 family, member A1 (ALDH7A1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,040.00 |
|
| SC321987 | ALDH7A1 (untagged)-Human aldehyde dehydrogenase 7 family, member A1 (ALDH7A1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,720.00 |
|
| SC327911 | ALDH7A1 (untagged)-Human aldehyde dehydrogenase 7 family member A1 (ALDH7A1) |
CNY 16,944.00 |
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