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VPS4B (NM_004869) Human Untagged Clone
CAT＃: SC127708
VPS4B (untagged)-Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MIG1; SKD1; SKD1B; VPS4-2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127708 sequence for NM_004869 edited (data generated by NextGen Sequencing)
ATGTCATCCACTTCGCCCAACCTCCAGAAAGCGATAGATCTGGCTAGCAAAGCAGCGCAA GAAGACAAGGCTGGGAACTACGAAGAAGCCCTTCAGCTCTATCAGCATGCTGTGCAGTAT TTTCTTCATGTCGTTAAATATGAAGCACAGGGTGATAAAGCCAAGCAAAGTATCAGGGCA AAGTGTACAGAATATCTTGATAGAGCAGAAAAACTAAAGGAGTACCTGAAAAATAAAGAG AAAAAAGCACAGAAGCCAGTGAAAGAAGGACAGCCGAGTCCAGCAGATGAGAAGGGGAAT GACAGTGATGGGGAAGGAGAATCTGATGATCCTGAAAAAAAGAAACTACAGAATCAACTT CAAGGTGCCATTGTTATAGAACGACCAAATGTGAAATGGAGTGACGTTGCTGGACTTGAA GGAGCCAAAGAAGCACTGAAAGAGGCTGTGATACTGCCTATTAAATTTCCTCATCTTTTT ACAGGCAAGAGAACACCTTGGAGGGGAATCCTATTATTTGGGCCGCCTGGAACAGGAAAG TCCTACTTAGCCAAAGCTGTAGCAACAGAAGCCAACAACTCAACATTTTTTTCAATATCT TCCTCTGATCTTGTTTCTAAGTGGCTAGGTGAAAGTGAAAAACTGGTTAAGAATTTATTC CAACTTGCCAGAGAGAACAAGCCCTCCATTATCTTCATTGATGAAATTGATTCTCTCTGT GGTTCAAGAAGTGAAAATGAAAGTGAAGCCGCACGTAGAATTAAGACGGAGTTCCTAGTG CAAATGCAAGGGGTTGGTGTAGACAATGATGGAATTTTGGTTCTGGGAGCTACAAATATA CCCTGGGTTCTGGATTCTGCCATTAGGCGAAGATTTGAGAAACGAATTTATATTCCCTTG CCGGAACCCCATGCCCGAGCAGCAATGTTTAAACTGCACCTAGGGACCACTCAGAACAGT CTCACGGAAGCAGACTTTCGGGAACTTGGGAGGAAAACAGATGGTTATTCAGGGGCAGAT ATAAGTATCATTGTACGTGATGCCCTTATGCAGCCTGTTAGGAAAGTACAGTCAGCTACT CATTTTAAAAAGGTTCGCGGACCTTCCCGAGCTGATCCTAACCATCTTGTAGATGATCTG CTAACACCTTGCTCTCCAGGTGACCCTGGTGCCATTGAAATGACATGGATGGATGTCCCT GGAGATAAACTTTTGGAGCCAGTTGTTTCCATGTCGGATATGTTGCGGTCACTATCTAAC ACAAAACCTACAGTCAATGAACATGACTTGTTGAAATTAAAGAAGTTTACAGAAGATTTT GGTCAAGAAGGCTAA Clone variation with respect to NM_004869.3 >OriGene 5' read for NM_004869 unedited
CGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGTCCGAGAACCACCGGCCTC CCCAGTTTGAGGGCTGTTACCCCGTGCGCGCTTCGACGTTGCTGCTGTTGGCTCTCCTCG CCCCTCGTTCCCTTGGGAACCGCCTGGGAACTCCGCCATGTCATCCACTTCGCCCAACCT CCAGAAAGCGATAGATCTGGCTAGCAAAGCAGCGCAAGAAGACAAGGCTGGGAACTACGA AGAAGCCCTTCAGCTCTATCAGCATGCTGTGCAGTATTTTCTTCATGTCGTTAAATATGA AGCACAGGGTGATAAAGCCAAGCAAAGTATCAGGGCAAAGTGTACAGAATATCTTGATAG AGCAGAAAAACTAAAGGAGTACCTGAAAAATAAAGAGAAAAAAGCACAGAAGCCAGTGAA AGAAGGACAGCCGAGTCCAGCAGATGAGAAGGGGAATGACAGTGATGGGGAAGGAGAATC TGATGATCCTGANAAAAAGAAACTACAGAATCAACTTCAAGGTGCCATTGTTATAGAACG ACCAAATGTGAAATGGAGTGACGTTGCTGGACTTGAAGGAGCCAAAGAAGCACTGGAAGA GGCTGTGATACTGCCTATTAATTNCCTCATCTTTNTACAGGGCAGAGAACACCTTGGNAG GGGATCCCNTATATTTGGCCGCCTGGAACAGGNAAGTCCTACTTAGCCAAAGCTGTAGCA ACAGAAGCCAANCCACTCACATTTTTTACAATATCTCCTCTGGATCTGNTNCTAAGTGGC TAGTGAAAGTGAAAAACTGGGTAAGAANTTATTNCNNACTGCCAGAGAGAACCAGCCCTC CATTATCTNCATTGATGAAAANTGATCTTCCTGTGGNTCAAGAAAGTGAAATGAAAGTGA GCCCCACGTANAATTAGACGGAGTCCTATGCCATGCAGGGGTNGNGTANCATGATGAATT TGGNTCTGGACTACAATTACC >OriGene 3' read for NM_004869 unedited
GCGGCCGCGGGGGCAGNCCGCANACTCTCCCNNNNGGTTTTTACTTGNNACGCGGCCGCT TTTNANATCGNGTTTTTTTTTTTTTTTTTTTTTAGGGTATATCAATTTACTTCACATAAG AGCATAATTCAGGCCTTTTCCATTTAATAACAAAAACAATCTGTACAGAAAACCCAAAGG CAACCACATAGCATATGTAAAATGTGCAAATACACTTTAAAATGCAAGTTATTCTATAGC ATTTGCAAGATAGAATTTCACTGTAATTAGGGAATCTAGTTCATCCTAACTTAATAGTCT TTTGCATGTATAGACAATGCAATTCTACAAGGCACAACTCAGCGTTGATGCTAAAGTATG AAACACATCCTCAGATTATTTATTTGAAAATATTAAAATAGCATCATGTTTATTATTTTT TAATGAGTCATGAGCTCATTTCTAAAGCTTCATAAAGCATTACACTGATAACATATGTGT GGTCAGGACAAACTGTTCCCTGAACTTAAAAGGTGAAGGACAAGACCCCATATTATTATC CTGTATTAAAAAAGGAAATATACATATATGTACACAGACACCCCATATCACAGACAAGAA ACTTCCCATAATTCAAAGGGAGACCATTTCCTTATTAGCAAAGGTGCGCATTACAGTATT TCATGACAGTTAAAAATTACACACCTACCACCTGTTTTTGGTCAATCTTGCTAAAAAAGA ACTGAAATAGACAATTTCTTCTTTAAGGTAAAGACAATGTCTAATTTAGAAAATCTTCCT CTTGAGATGCACAGTGAAAGGTATCAGNTAATTAATATTAAAGCTTACCGTTTTTGTTTT TTTTGAGACGAGTTTTATTCAGTCGCCAAGCTTGATGCAATGGGCTTCTTGGGCCCAAGC TGGATGCAATACGCGATTCAACTACTGGAACTCTTGCTTCAGTTCAGTGATTCTCCGCT |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_004869 |
| Insert Size | 3160 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004869.2, NP_004860.2 |
| RefSeq Size | 3337 bp |
| RefSeq ORF | 1335 bp |
| Locus ID | 9525 |
| UniProt ID | O75351 |
| Domains | AAA, AAA, MIT |
| Protein Families | Transcription Factors |
| Protein Pathways | Endocytosis |
| Gene Summary | The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208019 | VPS4B (Myc-DDK-tagged)-Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B) |
CNY 3,656.00 |
|
| RC208019L1 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208019L2 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B), mGFP tagged |
CNY 5,890.00 |
|
| RC208019L3 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208019L4 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B), mGFP tagged |
CNY 6,056.00 |
|
| RG208019 | VPS4B (tGFP-tagged) - Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B) |
CNY 5,256.00 |
|
| SC324083 | VPS4B (untagged)-Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B) |
CNY 3,656.00 |
