RAP1A (NM_002884) Human Untagged Clone
CAT#: SC127673
RAP1A (untagged)-Human RAP1A, member of RAS oncogene family (RAP1A), transcript variant 2
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_002884, the custom clone sequence may differ by one or more nucleotides
ATGCGTGAGTACAAGCTAGTGGTCCTTGGTTCAGGAGGCGTTGGGAAGTCTGCTCTGACAGTTCAGTTTG TTCAGGGAATTTTTGTTGAAAAATATGACCCAACGATAGAAGATTCCTACAGAAAGCAAGTTGAAGTCGA TTGCCAACAGTGTATGCTCGAAATCCTGGATACTGCAGGGACAGAGCAATTTACAGCAATGAGGGATTTG TATATGAAGAACGGCCAAGGTTTTGCACTAGTATATTCTATTACAGCTCAGTCCACGTTTAACGACTTAC AGGACCTGAGGGAACAGATTTTACGGGTTAAGGACACGGAAGATGTTCCAATGATTTTGGTTGGCAATAA ATGTGACCTGGAAGATGAGCGAGTAGTTGGCAAAGAGCAGGGCCAGAATTTAGCAAGACAGTGGTGTAAC TGTGCCTTTTTAGAATCTTCTGCAAAGTCAAAGATCAATGTTAATGAGATATTTTATGACCTGGTCAGAC AGATAAATAGGAAAACACCAGTGGAAAAGAAGAAGCCTAAAAAGAAATCATGTCTGCTGCTCTAG >OriGene 5' read for NM_002884 unedited
NGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGGGATCGTC AGTATTTAAACAGATCACATCATGCGTGAGTACAAGCTAGTGGTCCTTGGTTCAGGAGGC GTTGGGAAGTCTGCTCTGACAGTTCAGTTTGTTCAGGGAATTTTTGTTGAAAAATATGAC CCAACGATAGAAGATTCCTACAGAAAGCAAGTTGAAGTCGATTGCCAACAGTGTATGCTC GAAATCCTGGATACTGCAGGGACAGAGCAATTTACAGCAATGAGGGATTTGTATATGAAG AACGGCCAAGGTTTTGCACTAGTATATTCTATTACAGCTCAGTCCACGTTTAACGACTTA CAGGACCTGAGGGAACAGATTTTACGGGTTAAGGACACGGAAGATGTTCCAATGATTTTG GTTGGCAATAAATGTGACCTGGAAGATGAGCGAGTAGTTGGCAAAGAGCAGGGCCAGAAT TTAGCAAGACAGTGGTGTAACTGTGCCTTTTTTAGAATCTTCTGCAAAGTCAAAGATCAA TGTTAATGAGATATTTTATGACCTGGTCAGACAGATAAATAGGAAAACACCAGTGGAAAA GAAGAAGCCTAAAAAGAAATCATGTCTGCTGCTCTAGGCCCATAGTCAGCAGCAGCTCTG AGCCAGATTACAGGAATGAAGAACTGTTGCCTAATTGGAAAGTGCCAGCATTCCAGACTT CAAAAATAAAATCTGAGAGGCTTCTNCTGTTTATATATATGTGAAGATTTAGATCTTATA TGGTNNNGCACAGTCCTGGAGAAAAAAATGCTCTGTGTTNTCTCTTGGAAAATAGACATA GTTTTTCTCTTTGCATAGCAGTATTACAGATGTGAAATATACTGACTCTATATGATATAC AAAGAGCATGGTGCATTTCAATGTTAGATATGCTCTATATCAATGC |
Restriction Sites | NotI-NotI |
ACCN | NM_002884 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002884.2, NP_002875.1 |
RefSeq Size | 1812 bp |
RefSeq ORF | 555 bp |
Locus ID | 5906 |
UniProt ID | P62834 |
Domains | ras, RAN, RAS, RHO, RAB |
Protein Families | Druggable Genome |
Protein Pathways | Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Long-term potentiation, MAPK signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma |
Gene Summary | This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 4. Variants 1-5 encode the same protein. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215248 | RAP1A (Myc-DDK-tagged)-Human RAP1A, member of RAS oncogene family (RAP1A), transcript variant 2 |
CNY 2,400.00 |
|
RC215248L3 | Lenti ORF clone of Human RAP1A, member of RAS oncogene family (RAP1A), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC215248L4 | Lenti ORF clone of Human RAP1A, member of RAS oncogene family (RAP1A), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG215248 | RAP1A (tGFP-tagged) - Human RAP1A, member of RAS oncogene family (RAP1A), transcript variant 2 |
CNY 4,000.00 |