STAU2 (AK002152) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	39K2; 39K3
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK002152, the custom clone sequence may differ by one or more nucleotides ATGGCAAACCCAAAAGAGAAAACTGCAATGTGTCTGGTAAATGAGTTAGCCCGTTTCAATAGAGTCCAAC CCCAGTATAAACTTCTGAATGAAAGAGGGCCTGCTCATTCAAAGATGTTCTCAGTGCAGCTGAGTCTTGG TGAGCAGACATGGGAATCCGAAGGCAGCAGTATAAAGAAGGCTCAGCAGGCTGTTGCCAATAAAGCTTTG ACTGAATCTACGCTTCCCAAACCAGTTCAGAAGCCACCCAAAAGTAATGTTAACAATAACCCAGGCAGTA TAACTCCAACTGTGGAACTGAATGGGCTTGCTATGAAAAGGGGAGAGCCTGCCATCTACAGGCCATTAGA TCCAAAGCCATTCCCAAATTATAGAGCTAATTACAACTTTCGGGGCATGTACAATCAGAGGTATCATTGC CCAGTGCCTAAGATCTTTTATGTTCAGCTCACTGTAGGAAATAATGAATTTTTTGGGGAAGGAAAGACTC GACAAGCTGCTAGACACAATGCTGCAATGAAAGCCCTCCAAGCACTGCAGAATGAACCTATTCCAGAAAG ATCTCCTCAGAATGGTGAATCAGGAAAGGATATGGATGATGACAAAGATGCAAATAAGTCTGAGATCAGC TTAGTGTTTGAAATTGCTCTGAAGCGAAATATGCCTGTCAGTTTTGAGGTTATTAAAGAAAGTGGACCAC CACATATGAAAAGCTTTGTTACTCGAGTGTCAGTAGGAGAGTTCTCTGCAGAAGGAGAAGGAAATAGCAA AAAACTCTCCAAGAAGCGCGCTGCGACCACCGTCTTACAGGAGCTTAAAAAACTTCCACCTCTTCCTGTG GTGGAAAAGCCAAAACTATTTTTTAAAAAACGCCCTAAAACAATAGTAAAGGCCGGACCAGAATATGGCC AAGGGATGAACCCTATTAGCCGCCTGGCGCAAATTCAACAGGCCAAAAAGGAAAAGGAGCCGGATTATGT TTTGCTTTCAGAAAGAGGAATGCCTCGACGTCGAGAATTTGTGATGCAGGTGAAGGTAGGCAATGAAGTT GCTACAGGAACAGGACCTAATAAAAAGATAGCCAAAAAAAATGCTGCAGAAGCAATGCTGTTACAACTTG GTTATAAAGCATCCACTAATCTTCAGGATCAACTTGAGAAGACAGGGGAAAACAAAGGATGGAGTGGTCC AAAGCCTGGGTTTCCTGAACCAACAAATAATACTCCAAAAGGAATTCTTCATTTGTCTCCTGATGTTTAT CAAGAGATGGAAGCCAGCCGCCACAAAGTAATCTCTGGCACTACTCTAGGCTATTTGTCACCCAAAGATA TGAACCAACCTTCAAGCTCTTTCTTCAGTATATCTCCCACATCGAATAGTTCAGCTACAATTGCCAGGGA ACTCCTTATGAATGGAACATCTTCTACAGCTGAAGCCATAGGTTTAAAAGGAAGTTCTCCTACTCCCCCT TGTTCTCCAGTACAACCTTCAAAACAACTGGAATATTTAGCAAGGATTCAAGGCTTTCAGGCAGCCTTAA GTGCCTTGAAACAATTTTCTGAACAAGGACTGGATCCAATCGATGGAGCAATGAATATCGAAAAAGGTTC TCTTGAAAAACAAGCCAAGCATCTGAGAGAGAAAGCGGACAATAACCAGGCACCCCCGGGCTCCATCGCT CAGGACTGCAAGAAATCAAACTCGGCCGTCTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK002152 unedited ACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTGCCAATGTTGGAGCCGTCTGC AAAGTGTCCCCGGCAAGAAGAGGCTGCCTACCACAAGGACTTTAGCTTACTTTTTAAAGA TTGAAGAAAAAAAAGAAGACAGAAAAAGAAGAACTCAAAGATACACAAAGTAATTTGAAC CAAGGCTCAGAAGTTTTTGGAGCCGTGAGGGATACAGCAGTTTGGTCAATATTGTCTTAA CATGCTTCAAATAAATCAGATGTTCTCAGTGCAGCTGAGTCTTGGTGAGCAGACATGGGA ATCCGAAGGCAGCAGTATAAAGAAGGCTCAGCAGGCTGTTGCCAATAAAGCTTTGACTGA ATCTACGCTTCCCAAACCAGTTCAGAAGCCACCCAAAAGTAATGTTAACAATAACCCAGG CAGTATAACTCCAACTGTGGAACTGAATGGGCTTGCTATGAAAAGGGGAGAGCCTGCCAT CTACAGGCCATTAGATCCAAAGCCATTCCCAAATTATAGAGCTAATTACAACTTTCGGGG CATGTACAATCAGAGGTATCATTGCCCAGTGCCTAAGATCTTTTATGTTCAGCTCACTGT ANGAAATAATGAATTTTTTGGGGAAGGAAAGACTCGANCAGCTGCTAGACACAATGCTNG CATGAAAGCCCTCCAAGCACTGCAGAATGAACCTATTCCAGAAAGATCTNCTCAGAATGG TGAATCANNGAAGGATATGGATGATGANCAAGATGCANATAAGTCTGAGATCAAGCTTAG TGTTNNGAAANTGCTCTGAAGCGAAATATGCCCCTGTCAGTTTGAGGGTATTAAGAAAGT GGACACCACATATGAAAGCTTTGTACTCGAGTGTCAGTAGAGAGTCTCTGCAGAGGAGAA GGAATAGCANAAACTTTCAGAAGCGCGCTGCGACCACCGCTTAAGGAGCTTAAAACTCCA CTCTTNCTGTGGTGGAAAGCAAACTATTTTAAAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK002152 unedited GGCCTTAGAACCAACCCATTCTTACTGCATCTGCTCCACGCTGGCTTCCAACATGCTGGC CCGGAGCGTGGCTGGCTGGAAGCAACTCCAACAGGTTTTTCCCTTCCCCGTCATGTACAT TATTTATTTTTGATCCTACTCACTGTCCCAAGTCCAGAGGCAGTTACAAAAAACACTCTT GATGCAAACCGTGAGTGGCTACAACACACGGATGGGGGTGGGCGCGATTCCCACAACAGG GAGTGGAATCCGGGAAGATGATATATAGGGGCAAGACGCCCCCTTACTTGCTAAGAGTAT ATGGAGCTCAAAACCCACAATTGCTTTGTTTTGTTTCTCAGTTCCTGGAGTATGTTGAAA CTACTTGCTCTTAACATTAGTTCGTATTTTTCATCAGATATCTGACCTGATTTAAAACAT GTTTGTTTGCATACATCTTTTTGTAGTGCACCTTATAGTTATTCCTACTGATATAGGATG AAGATTATAAATATCTGCATAAAAAGAGAAACCATGTGACTCTATATGAAGACAACCATC ACATTCCACAGCACATCATTGGTTCATTTTCGAATTGTCAAGCAGTATTTGAAATGAGAG AGAGAGAGAATATAACTGAACACAAGCACCACGACAAAACAATCATAACAACAGAACCAA ACCCAACTTCTCTATTATTAATCATGTTAAAATTTTAGCTTTGTTTCTAACACTTTTCTT TACTTGTTATTTTAAGCTCCAGTAGCAGGATCAGATTTCTGCTGCCTCTAGGCAAATGAG TTATGATCTGATCTCGAATTCCAAGGGAAATGCTCAAGTTTTATTTTTCCCCATTGAATA AACATACCATGTATATTATCCTCTCGGGTAGAATAGTGCTGCCTTCCATAGACTCAATAA TGGTTTAGTCATCCATTTCCCTGACCACAACCN
Restriction Sites	NotI-NotI
ACCN	AK002152
Insert Size	2940 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK002152.1, BAA92111.1
RefSeq Size	2968 bp
RefSeq ORF	1713 bp
Locus ID	27067
Domains	DSRM
Protein Families	Transcription Factors
Gene Summary	Staufen homolog 2 is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. Staufen homolog 2 shares 48.5% and 59.9% similarity with drosophila and human staufen, respectively. The exact function of Staufen homolog 2 is not known, but since it contains 3 copies of conserved dsRNA binding domain, it could be involved in double-stranded RNA binding events. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

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