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G protein alpha S (GNAS) (NM_080426) Human Untagged Clone
CAT＃: SC127619
GNAS (untagged)-Human GNAS complex locus (GNAS), transcript variant 3
CNY 5,488.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; PITA3; POH; SCG6; SgVI |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_080426, the custom clone sequence may differ by one or more nucleotides
ATGGGCTGCCTCGGGAACAGTAAGACCGAGGACCAGCGCAACGAGGAGAAGGCGCAGCGTGAGGCCAACA AAAAGATCGAGAAGCAGCTGCAGAAGGACAAGCAGGTCTACCGGGCCACGCACCGCCTGCTGCTGCTGGG TGCTGGAGAATCTGGTAAAAGCACCATTGTGAAGCAGATGAGGATCCTGCATGTTAATGGGTTTAATGGA GACAGTGAGAAGGCAACCAAAGTGCAGGACATCAAAAACAACCTGAAAGAGGCGATTGAAACCATTGTGG CCGCCATGAGCAACCTGGTGCCCCCCGTGGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACAT CCTGAGTGTGATGAACGTGCCTGACTTTGACTTCCCTCCCGAATTCTATGAGCATGCCAAGGCTCTGTGG GAGGATGAAGGAGTGCGTGCCTGCTACGAACGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTACT TCCTGGACAAGATCGACGTGATCAAGCAGGCTGACTATGTGCCGAGCGATCAGGACCTGCTTCGCTGCCG TGTCCTGACTTCTGGAATCTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTTGACGTG GGTGGCCAGCGCGATGAACGCCGCAAGTGGATCCAGTGCTTCAACGATGTGACTGCCATCATCTTCGTGG TGGCCAGCAGCAGCTACAACATGGTCATCCGGGAGGACAACCAGACCAACCGCCTGCAGGAGGCTCTGAA CCTCTTCAAGAGCATCTGGAACAACAGATGGCTGCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAA GATCTGCTCGCTGAGAAAGTCCTTGCTGGGAAATCGAAGATTGAGGACTACTTTCCAGAATTTGCTCGCT ACACTACTCCTGAGGATGCTACTCCCGAGCCCGGAGAGGACCCACGCGTGACCCGGGCCAAGTACTTCAT TCGAGATGAGTTTCTGAGGATCAGCACTGCCAGTGGAGATGGGCGTCACTACTGCTACCCTCATTTCACC TGCGCTGTGGACACTGAGAACATCCGCCGTGTGTTCAACGACTGCCGTGACATCATTCAGCGCATGCACC TTCGTCAGTACGAGCTGCTCTAA >OriGene 5' read for NM_080426 unedited
GCAGTTCANATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCG AGCCCGCGCCCGGCCCGCCCGCCCGGCGCTGCCCCGGCCCTCCCGGCCCGCGTGAGGCCG CCCGCGCCCGCCGCCGCCGCAGCCCGGCCGCGCCCCGCCGCCGCCGCCGCCGCCATGGGC TGCCTCGGGAACAGTAAGACCGAGGACCAGCGCAACGAGGAGAAGGCGCAGCGTGAGGCC AACAAAAAGATCGAGAAGCAGCTGCAGAAGGACAAGCAGGTCTACCGGGCCACGCACCGC CTGCTGCTGCTGGGTGCTGGAGAATCTGGTAAAAGCACCATTGTGAAGCAGATGAGGATC CTGCATGTTAATGGGTTTAATGGAGAGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGC AACAGCGATGGTGAGAAGGCAACCAAAGTGCAGGACATCAAAAACAACCTGAAAGAGGCG ATTGAAACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCCGTGGAGCTGGCCAACCCC GAGAACCAGTTCAGAGTGGACTACATTCTGAGTGTGATGAACGTGCCTGACTTTGACTTC CCTCCCGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAAGGAGTGCGTGCCTGC TACGAACGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTACTTTCTGGACAAGATC GACGTGATCAAGCAGGCTGACTATGTGCCGAGCGATCAGGACCTGCTTCGCTGCCGTGTC CTGACTTCTGGAATCTTTGAGACCAAGTTTCCAGTGNACCGAAGTCACTTTCACATGTTT GACGCGGGTGGCCAGCGCGACGAACGCCGCAGTGGATCCACTGCTTCAACGATGCGACTG GCATCATTTTCGTGGGGGCCAGCAGCAGCTACACATGGCATCCCGGGAGACACN >OriGene 3' read for NM_080426 unedited
CCCATTCCGGAATTTTAATCTTGTTTTTTTTCATGCTGCACAACACAATATTTATTTCAG TTGTTTCTTTTATTTCATTTTATTTGTTTGCTGCTGCTGTTTTATTTATTTTTACTGAAA GTGAGAGGGAACTTTTGTGGCCTTTTTTCCTTTTTCTGTAGGCCGCCTTAAGCTTTCTAA ATTTGGAACATCTAAGCAAGCTGAAGGGAAAAGGGGGTTTCGCAAAATCACTCGGGGGAA GGGAAAGGTTGCTTTGTTAATCATGCCCTATGGTGGGTGATTAACTGCTTGTACAATTAC GTTTCACTTTTAATTAATTGTGCTTAAGGCTTTAATTAAATTTGGGGGTTCCCTTCTTAG AGCAGCTCGTACTGACGAAGGTGCATGCGCTGAATGATGTCACGGCAGTCGTTGAACACA CGGCGGATGTTCTCAGTGTCCACAGCGCAGGTGAAATGAGGGTAGCAGTAGTGACGCCCA TCTCCACTGGCAGTGCTGATCCTCAGAAACTCATCTCGAATGAAGTACTTGGCCCGGGTC ACGCGTGGGTCCTCTCCGGGCTCGGGAGTAGCATCCTCAGGAGTAGTGTAGCGAGCAAAT TCTGGAAAGTAGTCCTCAATCTTCGATTTCCCAGCACGGACTTTCTCAGCGAGCAGATCT TGCTTGTTGAGGAACAGGATCACAGAGATGGTGCGCAGCCATCTGTTGTTCCAGATGCTC TTGAAGAGGTTCAGAGCCTCCTGCACGCGGTTGGACTGGTTGTCCTCCTNGATGACCATG TTGTAGCTGCTGCTGGCCACCCCCAAGAGCATGCAGTCCCATCGTTGAAGCACTGGACCA CTTGCGGCGTTCATCGCGCTTGCCCCCACGTCAACATGTGGAAGCTGCCTTTGTCCCGCT GNACACTTGTTTCAAAGATTCCAAAGTCCAGACACCGCCCCCAACCAGTCCTGN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_080426 |
| Insert Size | 1800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_080426.1, NP_536351.1 |
| RefSeq Size | 1551 bp |
| RefSeq ORF | 1143 bp |
| Locus ID | 2778 |
| UniProt ID | O95467 |
| Domains | G-alpha |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Calcium signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Long-term depression, Melanogenesis, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection |
| Gene Summary | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (3) is biallelically expressed. It lacks an internal exon and uses an alternate splice site, compared to variant 1. It encodes isoform GNASS, also known as alpha-S1, which differs in an internal region and has identical N- and C-termini, compared to isoform GNASL. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214318 | GNAS (Myc-DDK-tagged)-Human GNAS complex locus (GNAS), transcript variant 3 |
CNY 5,856.00 |
|
| RC214318L3 | Lenti-ORF clone of GNAS (Myc-DDK-tagged)-Human GNAS complex locus (GNAS), transcript variant 3 |
CNY 5,890.00 |
|
| RC214318L4 | Lenti-ORF clone of GNAS (mGFP-tagged)-Human GNAS complex locus (GNAS), transcript variant 3 |
CNY 5,890.00 |
|
| RG214318 | GNAS (tGFP-tagged) - Human GNAS complex locus (GNAS), transcript variant 3 |
CNY 4,370.00 |
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