United States
Germany
Japan
United Kingdom
China
STT3A (NM_152713) Human Untagged Clone
CAT＃: SC127610
STT3A (untagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A)
CNY 5,168.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ITM1; STT3-A; TMC |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127610 sequence for NM_152713 edited (data generated by NextGen Sequencing)
ATGACTAAGTTTGGATTTTTGCGATTGTCCTATGAGAAGCAGGACACACTTTTGAAGCTT CTCATTCTGTCAATGGCTGCTGTATTATCCTTCTCCACTCGTCTGTTTGCTGTCCTGAGA TTTGAAAGTGTTATCCATGAGTTTGATCCGTACTTTAATTATCGGACTACCAGGTTCCTG GCTGAGGAGGGGTTTTATAAATTCCATAACTGGTTTGATGACCGAGCCTGGTACCCTTTG GGACGAATCATTGGAGGAACAATTTACCCAGGTTTAATGATCACCTCTGCTGCAATCTAC CATGTACTCCATTTTTTCCACATCACCATCGACATTCGGAATGTCTGTGTGTTCCTGGCC CCTCTCTTCTCCTCCTTCACCACCATCGTCACGTACCACCTTACCAAAGAGCTCAAGGAT GCAGGGGCTGGGCTTCTTGCTGCTGCCATGATTGCTGTAGTTCCTGGATATATCTCCCGA TCTGTGGCTGGCTCCTATGATAATGAAGGGATTGCCATCTTTTGCATGCTACTCACCTAC TACATGTGGATCAAGGCAGTAAAGACTGGTTCCATCTGTTGGGCAGCTAAGTGTGCCCTT GCTTATTTCTACATGGTCTCGTCATGGGGAGGTTATGTGTTCCTGATCAACTTAATTCCT CTCCACGTCCTCGTGCTGATGCTCACAGGCCGTTTCTCTCACCGGATCTATGTGGCCTAC TGTACTGTTTACTGCCTGGGCACTATACTTTCTATGCAGATCTCCTTTGTGGGTTTCCAG CCTGTCCTTTCATCAGAGCACATGGCAGCCTTTGGGGTCTTTGGTCTCTGCCAGATCCAT GCCTTTGTGGATTACCTGCGCAGCAAGTTGAATCCACAACAATTTGAAGTTCTTTTCCGG AGCGTCATCTCTCTGGTAGGCTTTGTCCTTCTCACCGTGGGAGCTCTCCTCATGCTGACA GGAAAAATATCTCCCTGGACGGGGCGTTTCTACTCGCTGCTGGATCCCTCTTATGCTAAG AACAACATCCCCATCATTGCTTCTGTGTCTGAGCATCAGCCCACAACCTGGTCCTCATAC TATTTTGACCTGCAGCTCCTCGTCTTCATGTTTCCAGTTGGCCTCTATTACTGCTTTAGC AACCTGTCTGATGCCCGGATTTTTATCATCATGTATGGTGTGACCAGCATGTACTTTTCA GCTGTAATGGTGCGTCTAATGCTAGTGTTGGCACCTGTTATGTGCATTCTCTCTGGCATT GGAGTCTCCCAGGTGCTGTCCACATACATGAAGAATCTGGACATAAGTCGTCCAGACAAG AAGAGCAAGAAGCAACAGGATTCCACCTACCCTATTAAGAATGAAGTGGCAAGTGGGATG ATACTGGTCATGGCTTTCTTTCTCATCACCTACACCTTTCATTCAACCTGGGTGACCAGT GAGGCCTACTCTTCTCCGTCCATTGTACTATCTGCCCGTGGTGGGGATGGCAGTAGGATC ATATTTGATGACTTCCGAGAAGCATATTATTGGCTTCGTCATAATACTCCAGAGGATGCG AAGGTCATGTCCTGGTGGGATTATGGCTATCAGATTACAGCTATGGCAAACCGAACAATT TTAGTGGACAATAACACATGGAATAATACCCATATTTCTCGAGTAGGGCAGGCAATGGCG TCCACAGAGGAAAAAGCCTATGAGATCATGAGGGAGCTCGATGTCAGCTATGTGCTGGTC ATTTTTGGAGGCCTCACTGGGTATTCCTCTGATGATATCAACAAGTTTCTTTGGATGGTC CGGATTGGAGGGAGCACAGATACAGGCAAACATATCAAGGAGAATGACTATTATACTCCA ACTGGGGAGTTCCGTGTGGACCGTGAAGGTTCTCCAGTGCTGCTCAACTGCCTCATGTAC AAGATGTGTTACTATCGCTTTGGACAGGTTTACACAGAAGCCAAGCGTCCTCCAGGCTTT GACCGTGTCCGAAATGCTGAGATTGGGAATAAAGACTTTGAGCTTGATGTCCTGGAGGAA GCATATACCACAGAACATTGGCTGGTCAGGATATACAAGGTAAAGGACCTGGATAATCGA GGCTTGTCAAGGACATAA Clone variation with respect to NM_152713.3 >OriGene 5' read for NM_152713 unedited
GATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGATGGCTGAGG GAGCCCCGCGGATCGTTTAGGAAAGCCGGCCAGCTGATCGTCGTGTGTTGCCACCCATTC ATGTCAAGATGACTAAGTTTGGATTTTTGCGATTGTCCTATGAGAAGCAGGACACACTTT TGAAGCTTCTCATTCTGTCAATGGCTGCTGTATTATCCTTCTCCACTCGTCTGTTTGCTG TCCTGAGATTTGAAAGTGTTATCCATGAGTTTGATCCGTACTTTAATTATCGGACTACCA GGTTCCTGGCTGAGGAGGGGTTTTATAAATTCCATAACTGGTTTGATGACCGAGCCTGGT ACCCTTTGGGACGAATCATTGGAGGAACAATTTACCCAGGTTTAATGATCACCTCTGCTG CAATCTACCATGTACTCCATTTTTTCCACATCACCATCGACATTCGGAATGTCTGTGTGT TCCTGGCCCCTCTCTTCTCCTCCTTCACCACCATCGTCACGTACCACCTTACCAAAGAGC TCAAGGATGCNAGGGGCTGGGCTTCTTGCTGCTGCCATGATTGCTTGTAGTTCCTGGATA TATCTCCCGATCTGGGGCTGGCTCCTATGATAATGAAAGGGATTGCCATCTTTTGCATGC TACTCACCTACTACATGGTGGATCAAGGCAGTAAGGACTGGTTCCATTCTGTTGGCAGCC TAAGTGTGCCCTTGCTTATTCTACATGGTCCGCTTGGGGGGGGGATTGTGGGTCCTGAAA ACTAATTCTCTCCCGTCCTCGGGCGAGCTACCGGGCCGTTCTTCCCGATTCTATTGGCCA CGGACTGGTTACTGCTGGCACTATCTTTAATCAATTCTTTTTGGGGGTCCCGCCCGCCTT CT >OriGene 3' read for NM_152713 unedited
GGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAATCCCCCCCTTTATTTGCCCAAAGAA AACCCCTGACTTTTTTTTGGGGAACCGGTTAAAAATTAAAAAAATTTTTTTTTTGGGCCC ACCCCTTTCTGCCCAAAAAAAAATTTCCAAACAATTTTGAGGCCCTCCAGTTTTGTTTTT ACAAACGGGTTTTTAAAAAAAAAAAAAAAAAAAATTTTTCAACGCCCTAAAGGGGAGGGG CTCAGGGGAAAACTTTTAAAAGGGGGGCGGGACTTTTTTGTCCTTGACAACCCTAAATTT TCCAGGCCCTTTACCTTGTTTTTCCGGACCACCCAAGGTTTGGGGGTATATGCTTCCTCC AAAACATAAAGCTAAAAGTTTTTTTTCCCAATTTAAAATTTTGGGACCCGGTAAAACCCT GGAGGACCCTTGGTTTTTGGGAAAACCTGCCCAAAGGGATAGTAACCCATTTTGTCCATG GGGGAGTTGACCACCCCTGGAAAACCTTCCGGGCCCACACGGAACTCCCCAGGGGGAGTA AAAAAGCCCTTCTCCTTGATATGTTGGCCTGTATTTGGGCCCCCTCCAATCCGGACCATC CAAAAAAACTTGTTGATTTCTTCAGAGGATTCCCCGGTGAGGGCCCCAAAAATGACCAGC CAATAGCTGGAATCNGGGCTCCCTCAAGAACCAATAGGCTTTTCCCTCTGGGACCCCTTT GCCGGCCCCACTGAAAAAATAGGGGTTTATCCATGGGGTTTTGCCCACTAAATGGCGGGT TGCCTTCCTGGATTTGAAGCCTTATCCCACCAGAATGACCTCTCATCTTGGGAGTTATGA CAAACCAATATAGCTTTGGAAGCATCAATGACCTCGGCCTCCCCCACGCAATATACTACG GAAAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_152713 |
| Insert Size | 2560 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_152713.2, NP_689926.1 |
| RefSeq Size | 2516 bp |
| RefSeq ORF | 2118 bp |
| Locus ID | 3703 |
| UniProt ID | P46977 |
| Domains | STT3 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| Gene Summary | The protein encoded by this gene is a catalytic subunit of the N-oligosaccharyltransferase (OST) complex, which functions in the endoplasmic reticulum to transfer glycan chains to asparagine residues of target proteins. A separate complex containing a similar catalytic subunit with an overlapping function also exists. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mutations in STT3A and STT3B cause two congenital disorders of glycosylation
,Shiteshu Shrimal, Bobby G. Ng, Marie-Estelle Losfeld, Reid Gilmore, and Hudson H. Freeze,
Hum. Mol. Genet., Jul 2013; 10.1093/hmg/ddt312.
,PubMed ID 23842455
[STT3A]
|
|
OST4 is a subunit of the mammalian oligosaccharyltransferase required for efficient N-glycosylation
,Audrey Dumax-Vorzet, Peristera Roboti, and Stephen High,
J. Cell Sci., Jun 2013; 126: 2595 - 2606.
,PubMed ID 23606741
[STT3A]
|
|
Keratinocyte-associated protein 2 is a bona fide subunit of the mammalian oligosaccharyltransferase
,Peristera Roboti and Stephen High,
J. Cell Sci., Jan 2012; 125: 220 - 232.
[STT3A]
|
|
Diagnosis of Suspicious Thyroid Nodules Using Four Protein Biomarkers
,Janete M. Cerutti, Flavia R.M. Latini, Claudia Nakabashi, Rosana Delcelo, Victor P. Andrade, Marcelo João Amadei, Rui M.B. Maciel, Flavio C. Hojaij, Donna Hollis, Jennifer Shoemaker, and Gregory J. Riggins,
Clin. Cancer Res. 2006 Jun 1;12(11 Pt 1):3311-8.
[STT3A]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201991 | STT3A (Myc-DDK-tagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A) |
CNY 5,168.00 |
|
| RC201991L1 | Lenti ORF clone of Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A), Myc-DDK-tagged |
CNY 7,568.00 |
|
| RC201991L2 | Lenti ORF clone of Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A), mGFP tagged |
CNY 7,700.00 |
|
| RC201991L3 | Lenti ORF clone of Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC201991L4 | Lenti ORF clone of Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A), mGFP tagged |
CNY 7,700.00 |
|
| RG201991 | STT3A (tGFP-tagged) - Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A) |
CNY 6,768.00 |
