ALP (PDLIM3) (BC001017) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ALP
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC001017, the custom clone sequence may differ by one or more nucleotides CGGAGTGGCTGCCCTGCGCGGGGACACTCAGAGCCCGGTGGGCGGGAGGAAGGCGGCATGCCCCAGACGG TGATCCTCCCGGGCCCTGCGCCCTGGGGCTTCAGGCTCTCAGGGGGCATAGACTTCAACCAGCCTTTGGT CATCACCAGGATTACACCAGGAAGCAAGGCGGCAGCTGCCAACCTGTGTCCTGGAGATGTCATCCTGGCT ATTGACGGCTTTGGGACAGAGTCCATGACTCATGCTGATGCGCAGGACAGGATTAAAGCAGCAGCTCACC AGCTGTGTCTCAAAATTGACAGGGGAGAAACTCACTTATGGTCTCCACAAGTATCTGAAGATGGGAAAGC CCATCCTTTCAAAATCAACTTAGAATCAGAACCACAGGAATTCAAACCCATTGGTACCGCGCACAACAGA AGGGCCCAGCCTTTTGTTGCAGCTGCAAACATTGATGACAAAAGACAGGTAGTGAGCGCTTCCTATAACT CGCCAATTGGGCTCTATTCAACTAGCAATATACAAGATGCGCTTCACGGACAGCTGCGGGGTCTCATTCC TAGCTCACCTCAAAAGACGGGAACTACTTTGAACACAAGCATAATATTCGGCCCAAACCTTTCGTGATCC CGGGCCGAAGCAGGTCATAAGCGCTGAGACTGTGGAGTTTGCAGTGCATGCTTCTTAAATCAGTTCTTAC TAATCATCAAGAAAAGTAACTGGTAGATGTTTGGAGTGGCTTTTCTCATTGCGAAGCTTATTTCAAAAGC AAAAAGAAACAAACTCACTTGGCAGAGAACAGAAGCCAAATAACTTGCATTTCCAAGAGTAAATATTGAC AAGATGACAGAGCTGTGAAATTGTATGCAATCACCACGTGTATTTATAGCGAACACCCCTGAGGCTCTGG GGATCCCATCCAGAACACTGTGACCTTTGAGTCGGGCAGTCAGTATGTCTATCCCAGGTCAGGAAAGCCC TGCCAGCAGGAGCGAGCATGGAAACCAGCCCCGGTTAGGAGGAAGGAATCAGGTGAACAAGCCAGAATGA TAAGGAGCAAGCTCTTCCTTCTCGTCTCTCAGTAGAGAAACTGATCATCTTCAGATCATCCTTTTCAAAT ATCTTCTCTAGAAATTGAACTCCAACCTAAGGGGAAAGAAAATAATGCCCTGTATTGCCAAATCAGGAAA CAGTGACTTCAGGAAACCTCTGTTCCATCCTTCTGACCTCAGCTAAGCCAGTCAGCCCACAGGAAGGAAG CTGGTGGTTAGACCGGATTGATTCCGACAGGAACATTGAATATGATTTACCGCAGCTGTTGCAAACTGAT GGCCCATAGGTGGATTTATTTCACAGGCATGTTTTATTTAATTCTTTTGTTTAAAAAAAAAAAAAGAATT TAAGTGCCTTTAGACAGAACATTTAAACTCACCATCTTGTCAAAATGCACATCACTCTTAAGTGTGTGAG ACACGGCCCATTTTCATACATGTGTGACCTCCTGGCCCCTCTAGACACCCGAGCTTATGACTCCTGTCAT AAGCTGCGGATAGCAGGTCATCCCTTATGTATGATTTCCATGTCCAGACCTTTCAGGAAGCTACAGGCAG AGCCAGGTGATTGTCTCAAAATATGCCAGACAATTCTTGTGATATCAGATCCAGAAAGGAGCCCTGGACC AGAGGAGCAGGAGAAAGGCGTTAAGAAGGCAACCATGAGCAAACACATCTTCCCCCCCCTAGAACGTGAG CTCAGTGTCTGCAGGGGCCTGGTCTGACTGGCTCAACATTATACCCCCAGCACCTAGAAATGAGACAGAA TAAATGCTCAATAAGCGACCATCTACAAACAGACAAAATAAATGCCCAATAAATGCTCACTGGATAAAGG AATCAAATCCTGAGGGTGCACAACATTTCCCAAGCAAGTGGCAAGAAGAGTCCAGAGCCGTCTCGCTCCC GATGCTGGCAGCTCTGTGTCCTCTCCTGCTTCTCTCTATGTGTTCCCTCGTTCATACTCTGTTCTCATCT GCATCTTTTCCGTTTCTCCACTTTCAACAGCTTCCCCACCCCAACCCAACCCCAATAACAAAACACTGGA TTTAAAGTTAAAAGGATATGATATTTTACTGTTGAATATATACAACTTCCCAAAGAAGCATAGTAAAATT AATCACTTGCATTCTTTTCAGGTTAGTGTGACTGAGTCCACCCACACAAGCTCTGGGGCTTCAGAAGGCT TGAATAAAGTGATACGTTAGAGTAACACATATCCTGCCTTTACTAAAAACCTATAACATTTAATTTTATA AAAAGTCAGGAAAGTCAGAGAATCCTAGGTATATAATCACCTTTTAAAAAAATTTTTCATTTTAACTTAA ACATAGGGATAATGGCAAGCCACTCATAATGTTGTCATTTGAAGATCACACCTTTTAATGTAGCTGAATT TGGAAGAGAGAAGGAGAGAAACAGAAATGAGAAGGTTTGCTTAAAAAACAGATGCCAGTGCACAAATCTT AAAGAATTATAAGGCCTGGTGCGATGGCTCACACCTGTAATCCCAGCACTTTGGGAGACAGAGGTGGGCG AATCACAAGGTCAGGAGTTCGAGACCATCCTGGCCAACATGGTGTAACCCCATGTCTACCAAAAATACAA AAAATTAGCTGGGCATGGTGGCGGGCACCTGTAATCCCAGCTACTGGGGAGGCTGAGGCAGGAGAATCGC CTGAGCCTGGGAGGCGGAGGTTGTAGTGAGCTGAGATCATGCCACTGCAACAAGCGTGAGACTCCATCTC AAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001017 unedited TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGTGGCTGCCC TGCGCGGGGACACTCAGAGCCCGGTGGGCGGGAGGAAGGCGGCATGCCCCAGACGGTGAT CCTCCCGGGCCCTGCGCCCTGGGGCTTCAGGCTCTCAGGGGGCATAGACTTCAACCAGCC TTTGGTCATCACCAGGATTACACCAGGAAGCAAGGCGGCAGCTGCCAACCTGTGTCCTGG AGATGTCATCCTGGCTATTGACGGCTTTGGGACAGAGTCCATGACTCATGCTGATGCGCA GGACAGGATTAAAGCAGCAGCTCACCAGCTGTGTCTCAAAATTGACAGGGGAGAAACTCA CTTATGGTCTCCACAAGTATCTGAAGATGGGAAAGCCCATCCTTTCAAAATCAACTTAGA ATCAGAACCACAGGACGGGAACTACTTTGAACACAAGCATAATATTCGGCCCAAACCTTT CGTGATCCCGGGCCGAAGCAGGTCATAAGCGCTGAGACTGTGGAGTTTGCAGTGCATGCT TCTTAAATCAGTTCTTACTAATCATCAAGAAAAGTAACTGGTAGATGTTTGGAGTGGCTT TTCTCATTGCGAAGCTTATTTCAAAAGCAAAAAGAAACAAACTCACTTGGCAGAAGACAG AAGCCAAAATAACTTGCATTTCCAAGAGTAAATATTGACAAGATGACAGAGCCTGTGAAA TTGTATGCAATCACCACGTGTATTTATAGCGAACACCCCTGAGGCTCTGGGGATCCCTAC AATACACTGTGACCTTTGAGTCGGGCAGTCAGTATGTCTATCCCCAGTCAAGTACAGCCC TGCCCACANGAGCGAGCCTGGGAACCCGCCCCGGTTAGGAGGAAGGATCCAGTGACAAGC CCTAATGAAAAGACCAACCTCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC001017 unedited ATGGAACGGCGGCCGCAATCTAGNGTCGAGTTTTTTTTTTTTTTTTTTGAGATGGAGTCT CACGCTTGTTGCAGTGGCATGATCTCAGCTCACTACAACCTCCGCCTCCCAGGCTCAGGC GATTCTCCTGCCTCAGCCTCCCCAGTAGCTGGGATTACAGGTGCCCGCCACCATGCCCAG CTAATTTTTTGTATTTTTGGTAGACATGGGGTTACACCATGTTGGCCAGGATGGTCTCGA ACTCCTGACCTTGTGATTCGCCCACCTCTGTCTCCCAAAGTGCTGGGATTACAGGTGTGA GCCATCGCACCAGGCCTTATAATTCTTTAAGATTTGTGCACTGGCATCTGTTTTTTAAGC AAACCTTCTCATTTCTGTTTCTCTCCTTCTCTCTTCCAAATTCAGCTACATTAAAAGGTG TGATCTTCAAATGACAACATTATGAGTGGCTTGCCATTATCCCTATGTTTAAGTTAAAAT GAAAAATTTTTTTAAAAGGTGATTATATACCTAGGATTCTCTGACTTTCCTGACTTTTTA TAAAATTAAATGTTATAGGTTTTTAGTAAAGGCAGGATATGTGTTACTCTAACGTATCAC TTTATTCAAGCCTTCTGAAGCCCCAGAGCTTGTGTGGGTGGACTCAGTCACACTAACCTG AAAAGAATGCAAGTGATTAATTNTACTATGCTTCTTTGGGAAGNTGTATATATTCAACAG TAAAATATCATATCCTTTTAACCTTTAATCCAGTGTTTTGGTATTGGNGTTGGGTTGNCG TGGGAAAGCTGTTGAAAAGTGGAAAACCGCAAAGAGCCGATGAGAACGAGTTGAACGAGG GAACACTACAGAGAGCGGGAAGGACCAAGCTGCCACCTCGGGACGAGACGCTCTGACTTT CTGCATTGCTTGGAAAGTTGGCACCTAGGATTTGATCTTATCGGGACATTATGCCTTTTT TGCTGTTGCAAGGCG
Restriction Sites	NotI-NotI
ACCN	BC001017
Insert Size	3180 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001017.1
RefSeq Size	2819 bp
RefSeq ORF	2819 bp
Locus ID	27295
Domains	PDZ, ZM
Gene Summary	The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Aberrant alternative splicing of this gene may play a role in myotonic dystrophy. [provided by RefSeq, Apr 2012]

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