GNPTAB (AK056137) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GNPTA; ICD
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK056137, the custom clone sequence may differ by one or more nucleotides AGACCCCGGCGGCGGCGGCGGCGGCTCAGGCTCCTCGGGGCGTGGCGTGGCGGTGAAGGGGTGATGCTGT TCAAGCTCCTGCAGAGACAGACCTATACCTGCCTGTCCCACAGGTATGGGCTCTACGTGTGCTTCTTGGG CGTCGTTGTCACCATCGTCTCCGCCTTCCAGTTCGGAGAGGTGGTTCTGGAATGGAGCCGAGATCAATAC CATGTTTTGTTTGATTCCTATAGAGACAATATTGCTGGAAAGTCCTTTCAGAATCGGCTTTGTCTGCCCA TGCCGATTGACGTTGTTTACACCTGGGTGAATGGCACAGATCTTGAACTACTGAAGGAACTACAGCAGGT CAGAGAACAGATGGAGGAGGAGCAGAAAGCAATGAGAGAAATCCTTGGGAAAAACACAACGGAACCTACT AAGAAGAGTGAGAAGCAGTTAGAGTGTTTGCTAACACACTGCATTAAGGTGCCAATGCTTGTCCTGGACC CAGCCCTGCCAGCCAACATCACCCTGAAGGACCTGCCATCTCTTTATCCTTCTTTTCATTCTGCCAGTGA CATTTTCAATGTTGCAAAACCAAAAAACCCTTCTACCAATGTCTCAGTTGTTGTTTTTGACAGTACTAAG GATGTTGAAGATGCCCACTCTGGACTGCTTAAAGGAAATAGCAGACAGACAGTATGGAGGGGCTACTTGA CAACAGATAAAGAAGTCCCTGGATTAGTGCTAATGCAAGATTTGGCTTTCCTGAGTGGATTTCCACCAAC ATTCAAGGAAACAAATCAACTAAAAACAAAATTGCCAGAAAATCTTTCCTCTAAAGTCAAACTGTTGCAG TTGTATTCAGAGGCCAGTGTAGCGCTTCTAAAACTGAATAACCCCAAGGATTTTCAAGAATTGAATAAGC AAACTAAGAAGAACATGACCATTGATGGAAAAGAACTGACCATAAGTCCTGCATATTTATTATGGGATCT GAGCGCCATCAGCCAGTCTAAGCAGGATGAAGACATCTCTGCCAGTCGTTTTGAAGATAACGAAGAACTG AGGTACTCATTGCGATCTATCGAGAGGCATGCACCATGGGTTCGGAATATTTTCATTGTCACCAACGGGC AGATTCCATCCTGGCTGAACCTTGACAATCCTCGAGTGACAATAGTAACACACCAGGATGTTTTTCGAAA TTTGAGCCACTTGCCTACCTTTAGTTCACCTGCTATTGAAAGTCACATTCATCGCATCGAAGGGCTGTCC CAGAAGTTTATTTACCTAAATGATGATGTCATGTTTGGGAAGGATGTCTGGCCAGATGATTTTTACAGTC ACTCCAAAGGCCAGAAGGTTTATTTGACATGGCCTGTGCCAAACTGTGCCGAGGGCTGCCCAGGTTCCTG GATTAAGGATGGCTATTGTGACAAGGCTTGTAATAATTCAGCCTGCGATTGGGATGGTGGGGATTGCTCT GGAAACAGTGGAGGGAGTCGCTATATTGCAGGAGGTGGAGGTACTGGGAGTATTGGAGTTGGACAGCCCT GGCAGTTTGGTGGAGGAATAAACAGTGTCTCTTACTGTAATCAGGGATGTGCGAATTCCTGGCTCGCTGA TAAGTTCTGTGACCAAGCATGCAATGTCTTGTCCTGTGGGTTTGATGCTGGCGACTGTGGGCAAGATCAT TTTCATGAATTGTATAAAGTGATCCTTCTCCCAAACCAGACTCACTATATTATTCCAAAAGGTGAATGCC TGCCTTATTTCAGCTTTGCAGAAGTAGCCAAAAGAGGAGTTGAAGGTGCCTATAGTGACAATCCAATAAT TCGACATGCTTCTATTGCCAACAAGTGGAAAACCATCCACCTCATAATGCACAGTGGAATGAATGCCACC ACAATACATTTTAATCTCACGTTTCAAAATACAAACGATGAAGAGTTCAAAATGCAGATAACAGTGGAGG TGGACACAAGGGAGGGACCAAAACTGAATTCTACAGCCCAGAAGGGTTACGAAAATTTAGTTAGTCCCAT AACACTTCTTCCAGAGGCGGAAATCCTTTTTGAGGATATTCCCAAAGAAAAACGCTTCCCGAAGTTTAAG AGACATGATGTTAACTCAACAAGGAGAGCCCAGGAAGAGGTGAAAATTCCCCTGGTAAATATTTCACTCC TTCCAAAAGACGCCCAGTTGAGTCTCAATACCTTGGATTTGCAACTGGAACATGGAGACATCACTTTGAA AGGATACAATTTGTCCAAGTCAGCCTTGCTGAGATCATTTCTGATGAACTCACAGCATGCTAAAATAAAA AATCAAGCTATAATAACAGATGAAACAAATGACAGTTTGGTGGCTCCACAGGAAAAACAGGTTCATAAAA GCATCTTGCCAAACAGCTTAGGAGTGTCTGAAAGATTGCAGAGGTTGACTTTTCCTGCAGTGAGTGTAAA AGTGAATGGTCATGACCAGGGTCAGAATCCACCCCTGGACTTGGAGACCACAGCAAGATTTAGAGTGGAA ACTCACACCCAAAAAACCATAGGCGGAAATGTGACAAAAGAAAAGCCCCCATCTCTGATTGTTCCACTGG AAAGCCAGATGAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK056137 unedited CTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGTGGCGGTGAAGGGGTGATGCTGT TCAAGCTCCTGCAGAGACAGACCTATACCTGCCTGTCCCACAGGTATGGGCTCTACGTGT GCTTCTTGGGCGTCGTTGTCACCATCGTCTCCGCCTTCCAGTTCGGAGAGGTGGTTCTGG AATGGAGCCGAGATCAATACCATGTTTTGTTTGATTCCTATAGAGACAATATTGCTGGAA AGTCCTTTCAGAATCGGCTTTGTCTGCCCATGCCGATTGACGTTGTTTACACCTGGGTGA ATGGCACAGACCTTGAACTACTGAAGGAACTACAGCAGGTCAGAGAACAGATGGAGGAGG AGCAGAAAGCAATGAGAGAAATCCTTGGGAAAAACACAACGGAACCTACTAAGAAGAGTG AGAAGCAGTTAGAGTGTTTGCTAACACACTGCATTAAGGTGCCAATGCTTGTCCTGGACC CAGCCCTGCCAGCCAACATCACCCTGAAGGACCTGCCATCTCTNTATCCTTCTTTTCATT CTGCCAGTGACATTNTCAATGTTGCAAAACCAAAAAACCCTTCTACCAATGTCTCAGTTG TTGNTTTTGACAGTACTAAGGGATGTGAAGATGCCCACTCTGNACTGCTTTAAGGAATAG CAGACAGACAGTATGGAGGGGCTACTTTGACACAGATAAAGAGTCCCTGGATTAGTGCTA AATGCAGATTTGGCTTTCCTGAGTGGATTTCCACCACATTCAGGAAACAAATTCACTAAA ACAAAATTGCCCAGAAATCTTTCTCTAAGTCAACTGNTGCAANTGATTCAAAGCCANTGT ANCGCTCTAAACTGNATACCCCAAGGATTTTCAGAAAATGATAGCAACCTAGAGACATGA CCCTTTGTGAAANGACTGACCATAGTCTGCATTTATTATGGNATCTGACGCATCAGCCAT CTAGCAGATGAGACTCTCTGCCATCGTTTAGATACAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK056137 unedited TTTAGCTTGNACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTCATC TGGCTTTCCAGTGGAACAATCAGAAATGGGGGCTTTTCTTTTGTCACATTTCCGCCTATG GTTTTTTGGGTGTGAGTTTCCACTCTAAATCTTGCTGTGGTCTCCAAGTCCAGGGGTGGA TTCTGACCCTGGTCATGACCATTCACTTTTACACTCACTGCAGGAAAAGTCAACCTCTGC AATCTTTCAGACACTCCTAAGCTGTTTGGCAAGATGCTTTTATGAACCTGTTTTTCCTGT GGAGCCACCAAACTGTCATTTGTTTCATCTGTTATTATAGCTTGATTTTTTATTTTAGCA TGCTGTGAGTTCATCAGAAATGATCTCAGCAAGGCTGACTTGGACAAATTGTATCCTTTC AAAGTGATGTCTCCATGTTCCAGTTGCAAATCCAAGGTATTGAGACTCAACTGGGCGTCT TTTGGAAGGAGTGAAATATTTACCAGGGGAATTTTCACCTCTTCCTGGGCTCTCCTTGTT GAGTTAACATCATGTCTCTTAAACTTCGGGAAGCGTTTTTCTTTGGGAATATCCTCAAAA AGGATTTCCGCCTCTGGAAGAAGTGTTATGGGACTAACTAAATTTTCGTAACCCTTCTGG GCCGTAGAATTCAGTTTTGGTCCCTCCCTTGTGTCCACCTCCACTGTTATCTGCATTTTG AACTCTTCATCGCTTGTATTTTGAAACGTGAGATAAAATGTTTTGTGGCGGCATTCATTC CACTGTGCATTATGAGGTGGATGGCTTTCCACCTTGTTGGCATACAACCATGTCCAATTA TTGGATTGTCACTTTGGCCCCCTTCACTCCTCTTTGGCTACTCTGCAAACTGAAAAAAGC GGCATCCCCTTTTGAAAATATATGAGTCTGGCTCGAAN
Restriction Sites	NotI-NotI
ACCN	AK056137
Insert Size	2760 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK056137.1, BAB71102.1
RefSeq Size	2603 bp
RefSeq ORF	2603 bp
Locus ID	79158
Domains	NL
Protein Families	Transmembrane
Protein Pathways	Lysosome
Gene Summary	This gene encodes two of three subunit types of the membrane-bound enzyme N-acetylglucosamine-1-phosphotransferase, a heterohexameric complex composed of two alpha, two beta, and two gamma subunits. The encoded protein is proteolytically cleaved at the Lys928-Asp929 bond to yield mature alpha and beta polypeptides while the gamma subunits are the product of a distinct gene (GeneID 84572). In the Golgi apparatus, the heterohexameric complex catalyzes the first step in the synthesis of mannose 6-phosphate recognition markers on certain oligosaccharides of newly synthesized lysosomal enzymes. These recognition markers are essential for appropriate trafficking of lysosomal enzymes. Mutations in this gene have been associated with both mucolipidosis II and mucolipidosis IIIA.[provided by RefSeq, May 2010]

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