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GPR85 (NM_018970) Human Untagged Clone
CAT＃: SC127481
GPR85 (untagged)-Human G protein-coupled receptor 85 (GPR85), transcript variant 2
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SREB; SREB2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127481 sequence for NM_018970 edited (data generated by NextGen Sequencing)
ATGGCGAACTATAGCCATGCAGCTGACAACATTTTGCAAAATCTCTCGCCTCTAACAGCC TTTCTGAAACTGACTTCCTTGGGTTTCATAATAGGAGTCAGCGTGGTGGGCAACCTCCTG ATCTCCATTTTGCTAGTGAAAGATAAGACCTTGCATAGAGCACCTTACTACTTCCTGTTG GATCTTTGCTGTTCAGATATCCTCAGATCTGCAATTTGTTTCCCATTTGTGTTCAACTCT GTCAAAAATGGCTCTACCTGGACTTATGGGACTCTGACTTGCAAAGTGATTGCCTTTCTG GGGGTTTTGTCCTGTTTCCACACTGCTTTCATGCTCTTCTGCATCAGTGTCACCAGATAC TTAGCTATCGCCCATCACCGCTTCTATACAAAGAGGCTGACCTTTTGGACGTGTCTGGCT GTGATCTGTATGGTGTGGACTCTGTCTGTGGCCATGGCATTTCCCCCGGTTTTAGACGTG GGCACTTACTCATTCATTAGGGAGGAAGATCAATGCACCTTCCAACACCGCTCCTTCAGG GCTAATGATTCCTTAGGATTTATGCTGCTTCTTGCTCTCATCCTCCTAGCCACACAGCTT GTCTACCTCAAGCTGATATTTTTCGTCCACGATCGAAGAAAAATGAAGCCAGTCCAGTTT GTAGCAGCAGTCAGCCAGAACTGGACTTTTCATGGTCCTGGAGCCAGTGGCCAGGCAGCT GCCAATTGGCTAGCAGGATTTGGAAGGGGTCCCACACCACCCACCTTGCTGGGCATCAGG CAAAATGCAAACACCACAGGCAGAAGAAGGCTATTGGTCTTAGACGAGTTCAAAATGGAG AAAAGAATCAGCAGAATGTTCTATATAATGACTTTTCTGTTTCTAACCTTGTGGGGCCCC TACCTGGTGGCCTGTTATTGGAGAGTTTTTGCAAGAGGGCCTGTAGTACCAGGGGGATTT CTAACAGCTGCTGTCTGGATGAGTTTTGCCCAAGCAGGAATCAATCCTTTTGTCTGCATT TTCTCAAACAGGGAGCTGAGGCGCTGTTTCAGCACAACCCTTCTTTACTGCAGAAAATCC AGGTTACCAAGGGAACCTTACTGTGTTATATGA Clone variation with respect to NM_018970.6 252 t=>c >OriGene 5' read for NM_018970 unedited
TTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTTACTGAGAGCTCC ATTTCTGGAAAGCCTTACAAGACTGAGGAATATCAGACTGCGAATCACCGGGAACGGTTC CTTTGCAGCACAGAAGCAATCTCTCTCCCCATCTTCGCATATTCTGATGGCAAAACAAGT GGAAGAAAAGAGGAAGCATGACTGCAGATCAGATCAGTTCTCTTTGTGGATTATATTTTC AGTAAAATGTATGGATCTATCTTTTCCTTGTTCTTATATCTAGATCATGAGACTTGACTG AGGCTGTATCCTTATCCTCCATCCATCTATGGCGAACTATAGCCATGCAGCTGACAACAT TTTGCAAAATCTCTCGCCTCTAACAGCCTTTCTGAAACTGACTTCCTTGGGTTTCATAAT AGGAGTCAGCGTGGTGGGCAACCTCCTGATCTCCATTTTGCTAGTGAAAGATAAGACCTT GCATAGAGCACCTTACTACTTCCTGTTGGATCTTTGCTGTTCAGATATCCTCAGATCTGC AATTTGTTTCCCATTTGTGTTCAACTCTGTCAAAAATGGCTCTACCTGNACTTATGGGAC TCTGACTTGCAAAGTGATTGCCTTTCTGGNGGTTTTGTCCTGTTTCCACACTGCTTTCAT GCTCTTCTGCATCAGTGTCACCAGATACTTAGCTATCGCCCATCACCGCTTCTATACAAG AGGCTGACCTTNTGGACGTGTCTGGCTGTGATCTGTATGGTGTGGACTCTGTCTGTGGCC ATGGCATTTCCCCCGTTTTAGACGTGGCACTTACTCATTCATAGGNANGAAAGATCATGC ACCTTNCCACACCGNTNCCTTAGGGCTAATGATCCCTTAAGATNATGCTGCTNCTGGCC >OriGene 3' read for NM_018970 unedited
TTATGGACCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCATTGAAACA TTTCATTGATTTATTTTCCTTATTTATAGGGATATTCTCTTTACAGAAGTACTATAGCAA TATGGAATTCTGTGTTATTAGATTCCTAATCTTAATTATCAAATACCAAAAAAAAATAAA CTTCACAAAAACCAACCAATAAACAAAACAACAGAAAGCAAACAAAAATAATAACTTTTT CTAGTTTTGTCATAATGTTAGTGTAAACCATTCTTCTTACAAGTTGTATATTTAGAACCC CAAATTTCTATGGTACTATGCTCCAAATTAAAGTGTGATGTTTATATTAAAATTCTGAGC CAATTAACTAAGCAGGTACACATCTCTAAATACTACTGGCATTTAAGAATAATAGAGGGA TGATTATAATAAACTACCAAATAATGCTTTTACAGATAATATTATGTCAGTTTATCAGCA TTATGTTGATTATCATTCATACATTTCCAGTCTGGAATATAGTAGCTACAAGAATCAAAA AGCAAAACCATAATAAAGCTAGTCTTCCTAGTTTGTGCTTGCCTCCCAAATAAAAAAACG TTGATATGTGCCAAAAATGTAAAACTTATTTCAAGAAATTAACCAGCTACACCGGTAAAT TTTGCAAATGCCACTTTATTTTGAAGTCACAATCTGTATCTAAAGATGAGACTACAGACT TTAATGTATTTTTAAATACCAGAACTCATATTTAAGCTTTCCAAGTTTAACCTGAATTAT TTAAATTACCATCCCCTGAATGGGCAGAAAGTTAAACAGACTGGGCCCTTCAAAAATAAA TGTTTACAAATGGGGAGGAGGCCTACTAAGGTGGGGGTCCCCTTGACGCTGACCTAAAAT AAAAAAAACAACTGGCCGGTTTTAAAATGTAAAAGGGGGCACCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018970 |
| Insert Size | 3780 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018970.3, NP_061843.2 |
| RefSeq Size | 4919 bp |
| RefSeq ORF | 1113 bp |
| Locus ID | 54329 |
| UniProt ID | P60893 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | Members of the G protein-coupled receptor (GPCR) family, such as GPR85, have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli, such as neurotransmitters, hormones, or light, induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins, or G proteins (Matsumoto et al., 2000 [PubMed 10833454]).[supplied by OMIM, Aug 2008] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All variants (1-4) encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205181 | GPR85 (Myc-DDK-tagged)-Human G protein-coupled receptor 85 (GPR85), transcript variant 2 |
CNY 3,656.00 |
|
| RC205181L1 | Lenti ORF clone of Human G protein-coupled receptor 85 (GPR85), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205181L2 | Lenti ORF clone of Human G protein-coupled receptor 85 (GPR85), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC205181L3 | Lenti ORF clone of Human G protein-coupled receptor 85 (GPR85), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205181L4 | Lenti ORF clone of Human G protein-coupled receptor 85 (GPR85), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG205181 | GPR85 (tGFP-tagged) - Human G protein-coupled receptor 85 (GPR85), transcript variant 2 |
CNY 5,256.00 |
