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PCF11 (NM_015885) Human Untagged Clone
CAT＃: SC127458
PCF11 (untagged)-Human PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae) (PCF11)
CNY 15,968.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127458 sequence for NM_015885 edited (data generated by NextGen Sequencing)
ATGTCAGAGCAGACGCCGGCCGAGGCCGGTGCTGCGGGGGCCCGGGAGGACGCCTGTCGG GATTATCAGTCATCGCTCGAAGACCTGACCTTCAATAGCAAGCCGCACATCAATATGCTG ACCATTCTAGCCGAGGAGAACCTGCCCTTCGCCAAGGAGATCGTCTCTCTCATCGAGGCC CAAACCGCCAAGGCTCCTTCCTCAGAGAAGCTTCCTGTTATGTACCTTATGGATTCTATT GTGAAAAACGTTGGAAGAGAGTATCTCACTGCCTTTACTAAAAATCTAGTTGCAACATTT ATTTGTGTGTTTGAAAAGGTGGATGAAAATACTAGGAAAAGTTTATTTAAGTTACGTTCT ACATGGGATGAAATATTCCCTTTGAAGAAACTTTATGCCCTGGATGTCAGAGTCAATTCA TTAGATCCTGCTTGGCCTATTAAACCTCTACCCCCCAATGTGAATACGTCTAGCATCCAT GTGAATCCTAAATTTTTAAATAAATCGCCCGAGGAGCCTTCAACACCTGGTACAGTGGTC AGTTCCCCTAGCATCTCCACTCCTCCAATTGTTCCTGATATACAAAAGAATCTTACACAA GAACAACTAATAAGGCAGCAGTTACTGGCAAAGCAAAAACAGTTGTTAGAACTTCAGCAG AAAAAGCTGGAGCTTGAGCTAGAGCAAGCTAAGGCACAGTTGGCAGTTTCTCTTAGTGTT CAGCAGGAAACATCCAATTTAGGTCCTGGATCTGCACCATCCAAATTACATGTTTCACAG ATTCCCCCTATGGCAGTTAAAGCTCCTCATCAGGTTCCTGTGCAATCTGAGAAAAGCCGT CCAGGACCATCCTTACAAATTCAGGATTTAAAAGGAACTAACCGGGATCCTCGTCTGAAC AGGATAAGCCAACATTCTCATGGAAAAGATCAGAGTCACAGGAAAGAATTTCTAATGAAC ACATTAAACCAGTCTGATACTAAGACAAGTAAAACTATACCCTCTGAAAAACTAAATTCA TCCAAGCAAGAAAAAAGTAAATCAGGTGAAAAAATAACCAAGAAAGAACTTGACCAATTA GATTCTAAATCGAAATCGAAATCGAAATCACCCTCACCTTTGAAAAACAAATTATCTCAC ACAAAAGACTTGAAAAATCAAGAATCGGAAAGTATGAGGTTGTCTGATATGAACAAGAGA GATCCAAGATTAAAAAAACATCTTCAGGATAAGACCGATGGCAAAGATGATGATGTGAAA GAGAAGAGAAAAACTGCAGAAAAAAAGGATAAAGATGAGCACATGAAGTCATCCGAACAC AGACTGGCTGGAAGTAGAAATAAAATCATAAATGGCATTGTACAAAAACAGGATACAATA ACAGAAGAGTCAGAAAAACAGGGGACAAAACCAGGGAGATCGAGTACTAGAAAGCGATCA AGATCTCGATCACCCAAGTCTAGGTCACCAATTATACATTCCCCAAAGAGAAGAGATAGG CGGTCACCCAAACGAAGGCAAAGAAGTATGTCTCCAACATCGACACCTAAAGCTGGAAAG ATTCGCCAATCTGGAGCTAAGCAGTCACATATGGAAGAGTTTACACCACCTTCTAGGGAA GACAGAAATGCTAAGAGAAGTACTAAACAGGATATTCGGGATCCAAGGCGAATGAAAAAG ACTGAAGAGGAGCGACCACAAGAAACTACAAATCAGCATTCTACAAAGTCAGGCACTGAA CCAAAGGAGAATGTAGAAAACTGGCAAAGTTCCAAGTCTGCCAAAAGATGGAAATCTGGT TGGGAAGAAAATAAAAGCTTACAACAGGTTGATGAACATAGTAAACCTCCTCATCTGAGG CATAGGGAGAGCTGGTCAAGCACTAAAGGAATTTTATCACCTCGAGCCCCAAAGCAGCAA CAGCATCGATTAAGTGTAGATGCCAATCTTCAGATTCCTAAAGAGTTAACTCTTGCAAGC AAAAGAGAATTACTTCAAAAGACGAGTGAACGTTTAGCATCTGGTGAAATTACACAGGAT GACTTCCTTGTTGTTGTGCATCAAATTCGACAGCTATTTCAGTATCAAGAAGGTGTGCGA GAAGAGCAGAGATCTCCATTCAATGATCGTTTTCCACTTAAGCGACCTCGATATGAAGAT TCAGATAAACCATTTGTAGATAGTCCAGCATCAAGATTCGCCGGCCTGGATACAAATCAG CGACTTACAGCTTTAGCTGAAGACAGACCGTTATTTGATGGACCTAGTAGGCCATCAGTA GCAAGAGATGGCCCAACGAAGATGATTTTTGAAGGACCCAATAAATTAAGCCCTCGAATT GATGGACCTCCCACACCAGCTTCTCTTCGGTTTGATGGGTCACCAGGACAAATGGGGGGA GGAGGCCCTTTGAGATTTGAGGGGCCACAAGGTCAGCTAGGAGGTGGGTGTCCTTTGAGA TTTGAAGGTCCTCCAGGACCAGTGGGGACACCTCTGCGGTTTGAGGGCCCAATTGGTCAA GCAGGAGGAGGTGGTTTTCGGTTTGAAGGTTCCCCTGGTCTGAGGTTTGAGGGATCTCCA GGTGGTTTGAGATTTGAGGGACCAGGAGGCCAGCCTGTGGGTGGTCTGAGGTTTGAGGGA CATCGTGGTCAACCTGTGGGTGGTCTAAGGTTTGAGGGACCTCATGGTCAGCCTGTGGGT GGACTTAGATTTGATAATCCCCGAGGTCAGCCTGTAGGTGGACTTAGATTTGAGGGGGGT CATGGTCCATCAGGGGCTGCGATTAGGTTTGATGGACCTCATGGTCAGCCAGGAGGTGGA ATCAGATTTGAGGGCCCTTTGCTACAGCAAGGGGTTGGAATGAGGTTTGAGGGCCCCCAT GGTCAGTCAGTAGCTGGTCTGAGATTTGAGGGACAACATAATCAACTTGGTGGGAACCTT AGGTTTGAGGGTCCACATGGTCAGCCAGGGGTTGGTATCAGGTTTGAAGGCCCTTTAGTC CAACAAGGAGGTGGAATGAGGTTTGAGGGTCCTTCTGTACCAGGAGGTGGCCTGAGAATT GAAGGGCCTCTGGGTCAAGGTGGTCCAAGATTTGAAGGTTGTCATGCTTTAAGGTTTGAT GGGCAGCCAGGTCAGCCGTCACTCTTGCCAAGATTTGATGGATTACATGGTCAGCCAGGT CCTAGATTTGAAAGGACTCCTGGTCAGCCAGGCCCTCAGAGGTTTGATGGACCACCTGGA CAGCAGGTTCAACCCAGATTTGACGGTGTACCTCAAAGATTTGATGGTCCACAACATCAG CAAGCATCAAGGTTTGATATTCCTCTTGGTCTTCAAGGCACAAGATTTGACAATCATCCT TCACAAAGGCTTGAATCAGTATCTTTCAATCAGACTGGTCCATATAATGATCCACCTGGC AATGCTTTTAATGCCCCATCCCAAGGACTACAGTTCCAAAGACATGAACAAATATTTGAT TCACCTCAAGGACCAAATTTTAATGGACCACATGGCCCTGGAAACCAGAGTTTCTCTAAT CCACTTAACAGAGCTTCTGGACACTATTTTGATGAAAAAAATCTTCAGAGTTCTCAATTT GGAAACTTTGGCAATATACCTGCTCCAATGACAGTAGGAAATATTCAGGCATCTCAACAG GTTCTGAGTGGTGTTGCTCAGCCAGTAGCTTTTGGTCAAGGACAACAGTTTTTACCAGTT CATCCACAAAATCCTGGATTTGTTCAGAATCCTTCAGGAGCCCTCCCTAAGGCATATCCT GATAATCATCTCAGTCAGGTGGATGTAAATGAATTGTTTTCAAAATTGCTAAAAACAGGA ATTCTCAAATTGTCCCAAACTGATTCAGCTACAACACAAGTAAGTGAAGTAACTGCTCAG CCTCCCCCTGAAGAGGAGGAAGATCAAAATGAAGATCAAGATGTTCCAGATCTTACTAAT TTTACAGTTGAAGAATTGAAACAACGTTATGACAGTGTTATAAATCGACTGTACACTGGT ATTCAGTGTTACTCTTGTGGAATGAGGTTTACAACATCACAGACAGATGTTTATGCAGAT CATTTGGACTGGCATTATCGGCAAAATAGAACTGAAAAGGATGTTAGCCGAAAAGTCACT CATAGACGTTGGTACTACAGTTTAACAGACTGGATAGAATTTGAGGAGATAGCTGATCTG GAAGAACGGGCAAAGAGCCAGTTTTTTGAAAAGGTGCATGAAGAAGTTGTGCTCAAAACT CAAGAGGCTGCTAAAGAAAAAGAGTTCCAAAGTGTACCTGCTGGACCAGCTGGAGCAGTT GAGAGTTGTGAAATCTGTCAAGAACAATTTGAACAATACTGGGATGAAGAAGAGGAGGAA TGGCATTTGAAAAATGCTATTAGAGTAGATGGAAAGATTTATCATCCATCATGTTATGAA GATTATCAAAATACATCTTCATTTGATTGTACACCATCTCCCAGCAAGACACCAGTTGAA AACCCCTTGAATATTATGTTGAACATTGTCAAAAACGAATTGCAGGAACCCTGTGACAGT CCCAAAGTTAAGGAAGAACGAATTGATACACCACCAGCTTGTACAGAGGAAAGCATAGCA ACACCCTCTGAAATTAAAACAGAAAATGACACAGTCGAGTCAGTTTAA Clone variation with respect to NM_015885.3 >OriGene 5' read for NM_015885 unedited
GTGGAGAAAGAAGCTTCTGTGGCGGCTGGAAGTGGACGGAGATCACCCGCGAGACGGCGG CGTTTCATACCCGAGGTTCCCCCTGTGTCGTCCCCCATCCCCCCTCCGCGGTCAGCATGT GGTGAAGCCGGAGCCGCGAGAGAGCCGGGGAGAGGAAGAGGAGTCGGAAGGGAGGCGGGG TATCCAGAGCGGCTTCAGCTTCAGCTGCAGCGGACCTCGGAGGGGGGCCGCGGCGCAATG TCAGAGCAGACGCCGGCCGAGGCCGGTGCTGCGGGGGCCCGGGAGGACGCCTGTCGGGAT TATCAGTCATCGCTCGAAGACCTGACCTTCAATAGCAAGCCGCACATCAATATGCTGACC ATTCTAGCCGAGGAGAACCTGCCCTTCGCCAAGGAGATCGTCTCTCTCATCGAGGCCCAA ACCGCCAAGGCTCCTTCCTCAGAGAAGCTTCCTGTTATGTACCTTATGGATTCTATTGTG AAAAACGTTGGAAGAGAGTATCTCACTGCCTTTACTAAAAATCTAGTTGCAACATTTATT TGTGTGTTTGAAAAGGTGGATGAAAATACTACGAAAAGTTTATTTAAGTTACGTTCTACA TGGGATGAAATATTCCCTTTGAAGAAACTTTATGCCCTGCATGTCAGAGTCAATTCATTA GATCCTGCTTGGCCCTATTAACCTCTTACCCCCCATGTGAATACGTCTAGCATTCATGTG AATCCTTAATTTTTAAATAAATCGCGCGAGGAAGCCTTCACACCTG >OriGene 3' read for NM_015885 unedited
TTTCTTTTTTACATGTTGAATTTAACTCTCAGGTGTCCATCTAAGTCAAAACCAACAAAA GAAAAAAGTTAACCATATCTCAAGCAAACATTCTTACAGATTCCCGTTACTTAAAATAGA AATATGTTCATCTGCATTAAATCATTTGACAATTTGAAAAAGTCTTTAAAAAAAAAATCA CCTTTAGTACTTAAATATATTTGTTAATTGTTTGATGGCATCAGTGTCCAAGTTCAAGAA TAAAACTTTGCATCATTAGATATTTTCAAAAGTGAACATCTGGATGGTTCTCTATACCTG GAGATGTGTTTGTGTATATATATATATATATCTGCAAAATATGTCCTATTTTATCTTTAA AATTTTTTCAAAATCTGGCTTTTCTATTTGAGAAAAGCCCTACGGTGCTCTCTCGGAACC CCCTTTTGAAGGATGAAACCTCAAACTTGCTTCTTTAATCAGTGGGAGGCTACTAGAAAA AAAATCTTATGTTGAGGTTGCTGTTCAATTCCATTTTTACCCAATAAAAAGTACAGAAAC TATTTGATGGTGTGCATAAATATTACACTCCATGAATAGCAAAAATATTTTTTATTTATA ATTTACAGTTGCACAGTGAAAAATCGTACATTTTTACACATACAATTTCATTTCGTTAAA TTTGCACACATCTACAGAACTTTCACACAACAAGCACCTGAAACTCTGCAGGCAGTAGGA AATGCAAATACTAAAATGTATTTATTAACCANATGATAGCTGAAGGCCCTGCCTCAGCCA GACAATNTATATAGATATGTCTATATATACATAANAAAATTCTTCACCTTCTAGNATCCA CAGCAGCTTTTTTAANAAAGAAAACATACTTTCTCATTNTATTTAACTGACTCGACTGGG TCATTTCCTGTTNTATTNCAGAGGNTGTGCTATGCTTTCCTCTGACAGCTGGNGNGGATC AATCCGTCTTCCTTACTTTGGAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015885 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015885.2, NP_056969.2 |
| RefSeq Size | 5834 bp |
| RefSeq ORF | 4668 bp |
| Locus ID | 51585 |
| UniProt ID | O94913 |
| Domains | RPR |
| Gene Summary | The protein encoded by this gene binds to CLP1 to form pre-mRNA cleavage factor IIm. The encoded protein is necessary for efficient Pol II transcription termination and may be involved in degradation of the 3' product of polyA site cleavage. [provided by RefSeq, Oct 2016] Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208928 | PCF11 (Myc-DDK-tagged)-Human PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae) (PCF11) |
CNY 15,960.00 |
|
| RC208928L1 | Lenti-ORF clone of PCF11 (Myc-DDK-tagged)-Human PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae) (PCF11) |
CNY 7,600.00 |
|
| RC208928L2 | Lenti-ORF clone of PCF11 (mGFP-tagged)-Human PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae) (PCF11) |
CNY 7,600.00 |
|
| RC208928L3 | Lenti-ORF clone of PCF11 (Myc-DDK-tagged)-Human PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae) (PCF11) |
CNY 7,600.00 |
|
| RC208928L4 | Lenti-ORF clone of PCF11 (mGFP-tagged)-Human PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae) (PCF11) |
CNY 7,600.00 |
|
| RG208928 | PCF11 (tGFP-tagged) - Human PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae) (PCF11) |
CNY 6,270.00 |
