ATF 4 (ATF4) (NM_182810) Human Untagged Clone

CAT#: SC127457

ATF4 (untagged)-Human activating transcription factor 4 (tax-responsive enhancer element B67) (ATF4), transcript variant 2



  "NM_182810" in other vectors (7)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
ATF4 mouse monoclonal antibody, clone OTI1H9
    • 100 ul

CNY 1,999.00
CNY 3,600.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CREB-2; CREB2; TAXREB67; TXREB
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC127457 sequence for NM_182810 edited (data generated by NextGen Sequencing)
ATGACCGAAATGAGCTTCCTGAGCAGCGAGGTGTTGGTGGGGGACTTGATGTCCCCCTTC
GACCCGTCGGGTTTGGGGGCTGAAGAAAGCCTAGGTCTCTTAGATGATTACCTGGAGGTG
GCCAAGCACTTCAAACCTCATGGGTTCTCCAGCGACAAGGCTAAGGCGGGCTCCTCCGAA
TGGCTGGCTGTGGATGGGTTGGTCAGTCCCTCCAACAACAGCAAGGAGGATGCCTTCTCC
GGGACAGATTGGATGTTGGAGAAAATGGATTTGAAGGAGTTCGACTTGGATGCCCTGTTG
GGTATAGATGACCTGGAAACCATGCCAGATGACCTTCTGACCACGTTGGATGACACTTGT
GATCTCTTTGCCCCCCTAGTCCAGGAGACTAATAAGCAGCCCCCCCAGACGGTGAACCCA
ATTGGCCATCTCCCAGAAAGTTTAACAAAACCCGACCAGGTTGCCCCCTTCACCTTCTTA
CAACCTCTTCCCCTTTCCCCAGGGGTCCTGTCCTCCACTCCAGATCATTCCTTTAGTTTA
GAGCTGGGCAGTGAAGTGGATATCACTGAAGGAGATAGGAAGCCAGACTACACTGCTTAC
GTTGCCATGATCCCTCAGTGCATAAAGGAGGAAGACACCCCTTCAGATAATGATAGTGGC
ATCTGTATGAGCCCAGAGTCCTATCTGGGGTCTCCTCAGCACAGCCCCTCTACCAGGGGC
TCTCCAAATAGGAGCCTCCCATCTCCAGGTGTTCTCTGTGGGTCTGCCCGTCCCAAACCT
TACGATCCTCCTGGAGAGAAGATGGTAGCAGCAAAAGTAAAGGGTGAGAAACTGGATAAG
AAGCTGAAAAAAATGGAGCAAAACAAGACAGCAGCCACTAGGTACCGCCAGAAGAAGAGG
GCGGAGCAGGAGGCTCTTACTGGTGAGTGCAAAGAGCTGGAAAAGAAGAACGAGGCTCTA
AAAGAGAGGGCGGATTCCCTGGCCAAGGAGATCCAGTACCTGAAAGATTTGATAGAAGAG
GTCCGCAAGGCAAGGGGGAAGAAAAGGGTCCCCTAG

Clone variation with respect to NM_182810.1
65 a=>c
>OriGene 5' read for NM_182810 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGTGGAAGAATCCCC
TGGTCTCCGTGAGCGTCCATTTTGTGGAACCTGAGTTGCAAGCAGGGAGGGGCAAATACA
ACTGCCCTGTTCCCGATTCTCTAGATGGCCGATCTAGAGAAGTCCCGCCTCATAAGTGGA
AGGATGAAATTCTCAGAACAGCTAACCTCTAATGGGAGTTGGCTTCTGATTCTCATTCAG
GCTTCTCACGGCATTCAGCAGCAGCGTTGCTGTAACCGACAAAGACACCTTCGAATTAAG
CACATTCCTCGATTCCAGCAAAGCACCGCAACATGACCGAAATGAGCTTCCTGAGCAGCG
AGGTGTTGGNTGGGGGACCTTGATGTCCCCCTTCGACCCGTCGGGTTTGGGGGCTGAAGA
AAGCCTAGGTCTCTTAGATGATTACCTGGAGGTGGCCAAGCACTTCAAACCTCATGGGTT
CTCCAGCGACAAGGCTAAGGCGGGCTCCTCCGAATGGCTGGCTGTGGATGGGTTGGGTCA
GTCCCTCCAACAACAGCAAGGAGGATGCCTTCTCCGGGACAATTGGNATGTTGGAGAAAA
TGGATTGAAAGAGTTCGACTTGGATGCCCTGTTGGGGTATAGATGACCTGGAAACCATGC
CAGATGACCTTCTGACCACGTTGGATGACACTTGTGATCTCTTTGCCCCCCTAGTCCANG
AGACTNAATAGCAGCCCCCCCAGACGGTGAACCCANTGGCCATCTCCCAGAAAGTTTACC
ANACCCGACCAGGTTGCCCCCTTCACCTTCTACACCTCTTCCCTTCCCCAAGGGGGCCTG
TCCTCCTCCAGACATNCCCTTANTTAAAACCGGCCGGGGAGGGGTATCCTGGAGAACAAG
GA
>OriGene 3' read for NM_182810 unedited
NNNNNNNNNNNNNNNTTTTAACTTTGAACCGCGGNCCGCATNCTANGATCGAGTTTTTTT
TTTTTTTTTTTACTTTCCCTACAAAAAATTTATTGGGAACACACAGCTACAGCACTCTAT
GTACAAGCACATTGACGCTCCTGACTATCCTCAACTAGGGGACCCTTTTCTTCCCCCTTG
CCTTGCGGACCTCTTCTATCAAATCTTTCAGGTACTGGATCTCCTTGGCCAGGGAATCCG
CCCTCTCTTTTAGAGCCTCGTTCTTCTTTTCCAGCTCTTTGCACTCACCAGTAAGAGCCT
CCTGCTCCGCCCTCTTCTTCTGGCGGTACCTAGTGGCTGCTGTCTTGTTTTGCTCCATTT
TTTTCAGCTTCTTATCCAGTTTCTCACCCTTTACTTTTGCTGCTACCATCTTCTCTCCAG
GAGGATCGTAAGGTTTGGGACGGGCAGACCCACAGAGAACACCTGGAGATGGGAGGCTCC
TATTTGGAGAGCCCCTGNTANAGGGGCTGTGCTGAGGAGACCCCANAAAGACTCTGGGCT
CATACAGATGCCCTATCATTATCTGAAGGGGTGTCTTCCTCCTTTATGCACTGAGGGAAC
ATGGCAACGTAAGCANTGTANTCCTGCCTTCCTATCTTCTTTAATGATATCCACTTAACT
GGCCANCTTTAAACTAAAGGAATGATCTTGAGGGGAAGACAGGACCCCCGGGGAAAGGGG
AAAAGTTTGAAAAATGGGAAGGGGGCACCCTGGTCGGTTTTGGAAAACTTTTGGGAAAAT
GCCAATGGGGTTACCGCTGGGGGGGCCTCTTTTTAATCTCCGGGACTTAGGGGGCAAAAA
ACACCAGTGTCCCCCCACGTGGCAAAAGCAATTGGCTGGGGTCCCGGCATTTTACCCCAG
GGGGTTCCAAGGAAAACCCTTAAACCATTTTCCCAAACCAATTTGGCCCGAAAAGCTTCT
CA
Restriction Sites NotI-NotI     
ACCN NM_182810
Insert Size 1410 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_182810.1, NP_877962.1
RefSeq Size 1420 bp
RefSeq ORF 1056 bp
Locus ID 468
UniProt ID P18848
Protein Families Transcription Factors
Protein Pathways GnRH signaling pathway, Long-term potentiation, MAPK signaling pathway, Neurotrophin signaling pathway, Prostate cancer
Gene Summary This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]
Transcript Variant: This variant (2) lacks an internal segment in the 5' UTR, compared to variant 1. The protein translation of this variant is regulated by two upstream open reading frames (PMID: 15277680). Both variants 1 and 2 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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