Rad6 (UBE2A) (NM_181777) Human Untagged Clone

CAT#: SC127386

UBE2A (untagged)-Human ubiquitin-conjugating enzyme E2A (UBE2A), transcript variant 3



  "NM_181777" in other vectors (4)

CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
UBE2A Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HHR6A; MRXS30; MRXSN; RAD6A; UBC2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_181777, the custom clone sequence may differ by one or more nucleotides


ATGTTCCATCCAAATGTCTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCAA
CCTATGATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGATGAACCCAATCCCAATAGTCCAGC
AAACAGCCAGGCTGCTCAGCTGTACCAGGAGAACAAACGGGAATATGAAAAGCGTGTTTCTGCAATAGTA
GAACAAAGCTGGCGTGATTGTTGA


>OriGene 5' read for NM_181777 unedited
TCATAATTTGTATACGACTCACTATAGGCGGCACGCGAATTCGCACCAGGCGGTTCCTCT
GGGTGCTTCCGCCTCCCCTTCTCCTGCTTCTCCAGCCTCTTCGGCCTCCTCGCCCGCCGC
GGGAACCCGAGACCCCAGTGTATGCCCCACCCCTGACCCCGCTCGCGACATGTCCACCCC
GGCTCGGCGGCGCCTCATGCGGGACTTCAAGAGGTTGCAGGAGGATCCTCCAGCCGGAGT
CAGCGGGGCTCCGTCCGAGAACAACATAATGGTGTGGAACGCGGTCATTTTCGGGCCTGA
AGGGACCCCGTTTGAGGATGGAACATTTAAACTTACAATAGAATTCACTGAAGAATATCC
AAATAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCCATCCAAATGTCTATGCAGA
TGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCAACCTATGATGTGTCTTC
CATTCTAACATCCATACAGTCTCTGTTGGATGAACCCAATCCCAATAGTCCAGCAAACAG
CCAGGCTGCTCAGCTGTACCAGGAGAACAAACGGGAATATGAAAAGCGTGTTTCTGCAAT
AGTAGAACAAAGCTGGCGTGATTGTTGACCCCAGGTACAGTTTATAGAAGCTGGCCATAA
GATAAAATATATTGATGTGTTTGTCACCTCCCTACTCCTGTCATTACATTTACTGTATTA
AAAGCGAAATAACTGGTGTGCTGTTTCCATCCTTCTTGCCAAGGTTTACTACCCCTTCTA
CCCTCTCCTTAAACATCAGAAACACCCTCTATGAAATAAATGTACTGTACCTGGGTTACT
TNGCAAAATACTATGGCTCAGNTNNTCTGTTGGATTCATTTCCAGNTTCAGGCAGNTATT
TTTATTATGGACTCTAAGCTTTAGAAGAAATGTATACAGAAGTGCTATGCTNACCTGAGA
C
Restriction Sites NotI-NotI     
ACCN NM_181777
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_181777.1, NP_861442.1
RefSeq Size 2678 bp
RefSeq ORF 234 bp
Locus ID 7319
Protein Families Druggable Genome
Protein Pathways Ubiquitin mediated proteolysis
Gene Summary The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Transcript Variant: This variant (3) lacks several 5' exons but has an alternate 5' segment, as compared to variant 1. It uses a downstream in-frame start codon and encodes isoform 3, which has a shorter N-terminus than that of isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...