United States
Germany
Japan
United Kingdom
China
UBE2H (NM_182697) Human Untagged Clone
CAT＃: SC127291
UBE2H (untagged)-Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 2
CNY 1,200.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | E2-20K; GID3; UBC8; UBCH; UBCH2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127291 sequence for NM_182697 edited (data generated by NextGen Sequencing)
ATGTCATCTCCCAGTCCGGGCAAGAGGCGGATGGACACGGACGTGGTCAAGCTCATCGAG AGTAAACATGAGGTTACGATCCTGGGAGGACTTAATGAATTTGTAGTGAAGTTTTATGGA CCACAAGGAACACCATATGAAGGCGGAGTATGGAAAGTTAGAGTGGACCTACCTGATAAA TACCCTTTCAAATCTCCATCTATAGATCTTACCAATATATTTGAGTCCTTCCTGCCTCAG TTATTGGCCTATCCTAACCCCATAGATCCTCTCAATGGTGACGCTGCAGCCATGTACCTC CACCGACCAGAAGAATACAAGCAGAAAATTAAAGAGTACATCCAGAAATACGCCACGGAG GAGGCGCTGAAAGAACAGGAAGAGGGTACCGGGGACAGCTCATCGGAGAGCTCTATGTCT GACTTTTCCGAAGATGAGGCCCAGGATATGGAGTTGTAG Clone variation with respect to NM_182697.2 >OriGene 5' read for NM_182697 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGGCCGACTGAG CAGGAGTCGTCCGCTTATAGTGGAGGCTGCTAGGAGCCGGTCAGAGGGTGAGTGGGGAGC AGGCGAGCGAGCCACAGCAGGCAGGAGGGAGCGTCGCGCGGCGCAGAGGAGCGCCGGGGC ATGGCCGCGGCGGAACCACAGCCGGAGAGGGGCAGCCCGAGCCGGGCGCCGCAGGGTCCC CACCCCCACCCGGCCGGACAGTGCCCGGTGTTCCCCCGTGCGGGGGGCGGCGGCGGCGGC TGACGGGACCGCCGGGACCGCGGGGGTGTTGCCACCTCCACCGAGGAGCCGGCGCGGTCG CGGGCTCCTCAGAGCCGGGGCCCCGGGCCCGTGACAGACGGTCCGAGGAATGGAGAGAGG CGGCGGCGACACCATGTCATCTCCCAGTCCGGGCAAGAGGCGGATGGACACGGACGTGGT CAAGCTCATCGAGAGTAAACATGAGGTTACGATCCTGGGAGGACTTAATGAATTTGTAGT GAAGTTTTATGGACCACAACGAACACCATATGAAGGCGGAGTATGGAAAGTTAGAGTGGA CCTACCTGATAAATACCCTTTCAAATCTCCATCTATAGGATTCATGAATAAAATTTTCCA TCCCAACATTGATGAAGCGTCACGAACTGCGTGTCTAGATGTAATTAATCAAACTTGGAC AGCTCTCTATGATCTTACCAATATATTTGAGTGCTTGCTGCCTCAGTTATTGGCCTATCC TAACCCCATAGATCCTCTGCATGGATGACGCTGCAGGCATGTACCTTCACCGACCAGATG AATACAGCNGAATATGAAGAGTGCATTCAGAATTACGCACNGGAGGATGCGCTGCAAGAA >OriGene 3' read for NM_182697 unedited
ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAAAAAAAACCTTTTGC TTCATGCCCGTAATTAACATAAAGTAAGTAAATTCTTTGTCTTTTTATTTAGGAAAATAA TCATGCTAAAAGCAAGTTGTACTTTATATAGATTTTCAAACAAAAGATTGATTGGGCTTT TTGAATAAAAAAGATAGGGTATCTTCCAACCTCACTGACTTAAACCTTGTGGAATACAGC ACTTCTAAACTAAAAACACACGTCAAAAAGAAAAATTAAATAATCCCAGTGGGATGCTCA AAAATCGTCTTTAGAAGGTTTTAGGATTGGCTAATTCTGCTATTATCTTATTGGCTGCGG CAAAAAAAGAACACAGAGCTGCTGTCTAAAATTTCTCCAAACTCCCCTCCGAGTTCAGCA GGCAACACTCAGATTTGAAAGAAACTCTGCAGCAACCTGGACATATGGTTAGCTGAGTCG ACATTTGATTAGCAGGCATTTTAAACTGATTTCCTCTTAACATACGCAGGAGAGAAAACA TTTTCTTCCTGCAGGGCATGTTCTACTACATCATATTTCTGAATCCCTTCTTCCTATTCG GTCACTCTCTGAAAAAAGCTGGTTTGTTTTTCCCATGTAGAATCACTCTGGGTACAAGAA CCTTTATTTTGTCCCATTATTATTTGTTTTAATNTAGAACCCTGTGTTGGGTCTAANATA CCCATTTCCCACTCCCACCAGCAGATGATGACCAAGCTGCTGCCCAGAGCAGGAGAAAAA GGGAAAACCTAACCATAACTGTAGGCCAGTACCCAGGCCTTTGCATTCCCACGTGGGTCG CCTCCTAAGTTTAATGCTCATGACCGCTTGTCCACGAAGCGGCCGCCTTTGGTAGGGAAT ATAAATCTGAACCTTTTTAAACAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_182697 |
| Insert Size | 2820 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_182697.1, NP_874356.1 |
| RefSeq Size | 2635 bp |
| RefSeq ORF | 459 bp |
| Locus ID | 7328 |
| UniProt ID | P62256 |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (2) lacks two consecutive in-frame exons in the coding region, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213095 | UBE2H (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 2 |
CNY 1,200.00 |
|
| RC213095L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213095L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG213095 | UBE2H (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 2 |
CNY 4,370.00 |
