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HLAE (HLA-E) (NM_005516) Human Untagged Clone
CAT＃: SC127254
HLA (untagged)-Human major histocompatibility complex, class I, E (HLA-E)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HLA-6.2; QA1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127254 sequence for NM_005516 edited (data generated by NextGen Sequencing)
ATGGTAGATGGAACCCTCCTTTTACTCCTCTCGGAGGCCCTGGCCCTTACCCAGACCTGG GCGGGCTCCCACTCCTTGAAGTATTTCCACACTTCCGTGTCCCGGCCCGGCCGCGGGGAG CCCCGCTTCATCTCTGTGGGCTACGTGGACGACACCCAGTTCGTGCGCTTCGACAACGAC GCCGCGAGTCCGAGGATGGTGCCGCGGGCGCCGTGGATGGAGCAGGAGGGGTCAGAGTAT TGGGACCGGGAGACACGGAGCGCCAGGGACACCGCACAGATTTTCCGAGTGAATCTGCGG ACGCTGCGCGGCTACTACAATCAGAGCGAGGCCGGGTCTCACACCCTGCAGTGGATGCAT GGCTGCGAGCTGGGGCCCGACGGGCGCTTCCTCCGCGGGTATGAACAGTTCGCCTACGAC GGCAAGGATTATCTCACCCTGAATGAGGACCTGCGCTCCTGGACCGCGGTGGACACGGCG GCTCAGATCTCCGAGCAAAAGTCAAATGATGCCTCTGAGGCGGAGCACCAGAGAGCCTAC CTGGAAGACACATGCGTGGAGTGGCTCCACAAATACCTGGAGAAGGGGAAGGAGACGCTG CTTCACCTGGAGCCCCCAAAGACACACGTGACTCACCACCCCATCTCTGACCATGAGGCC ACCCTGAGGTGCTGGGCCCTGGGCTTCTACCCTGCGGAGATCACACTGACCTGGCAGCAG GATGGGGAGGGCCATACCCAGGACACGGAGCTCGTGGAGACCAGGCCTGCAGGGGATGGA ACCTTCCAGAAGTGGGCAGCTGTGGTGGTGCCTTCTGGAGAGGAGCAGAGATACACGTGC CATGTGCAGCATGAGGGGCTACCCGAGCCCGTCACCCTGAGATGGAAGCCGGCTTCCCAG CCCACCATCCCCATCGTGGGCATCATTGCTGGCCTGGTTCTCCTTGGATCTGTGGTCTCT GGAGCTGTGGTTGCTGCTGTGATATGGAGGAAGAAGAGCTCAGGTGGAAAAGGAGGGAGC TACTCTAAGGCTGAGTGGAGCGACAGTGCCCAGGGGTCTGAGTCTCACAGCTTGTAA Clone variation with respect to NM_005516.5 >OriGene 5' read for NM_005516 unedited
TCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGAGGCTGGGATCATGGTAGATGGAAC CCTCCTTTTACTCCTCTCGGAGGCCCTGGCCCTTACCCAGACCTGGGCGGGCTCCCACTC CTTGAAGTATTTCCACACTTCCGTGTCCCGGCCCGGCCGCGGGGAGCCCCGCTTCATCTC TGTGGGCTACGTGGACGACACCCAGTTCGTGCGCTTCGACAACGACGCCGCGAGTCCGAG GATGGTGCCGCGGGCGCCGTGGATGGAGCAGGAGGGGTCAGAGTATTGGGACCGGGAGAC ACGGAGCGCCAGGGACACCGCACAGATTTTCCGAGTGAATCTGCGGACGCTGCGCGGCTA CTACAATCAGAGCGAGGCCGGGTCTCACACCCTGCAGTGGATGCATGGCTGCGAGCTGGG GCCCGACGGGCGCTTCCTCCGCGGGTATGAACAGTTCGCCTACGACGGCAAGGATTATCT CACCCTGAATGAGGACCTGCGCTCCTGGACCGCGGTGGACACGGCGGCTCAGATCTCCGA GCANAAGTCANATGATGCCTCTGAGGCGGAGCACCAGAGAGCCTACCTGGAAGACACATG CGTGGAGTGGGCTCACAATACCTGGAGAAGGGAAGGAGACGCTGCTTCACCTGGAGCCCC CAAAGACACACGTGACTCACCCCCCATCTCTGACATGAGGCCACCCTGAGTGCTGGCCCC TGGCTTNCTACCTGCGAGATACACTGACCTGCCACAGATGGGGAGGCCATACCAGACACG AGCTCGTGAGACAGCCTGCAGGGATGGACCTTCANAGTGGCACTGGGTGGTGCCTCTTGA AGAACNAGATCCGTGCATTGCACATGAGGGCTCCCGAGCCTACCTGAATGGAGCGTTCCA GCACATCCATGGGGATATGCTGCTGGTNNCTGATCGGCCTGACTGGTGTGTGGATGAGAA AAC >OriGene 3' read for NM_005516 unedited
CTATGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTTTTATTTT TTGAGATGGCGTTTCACCCTGTCACCCAGGCTGGAGTGCAGTCGCACAATCCTGGCTCAC TGCAACCTCCGCCTCCCGGGTTGACGCCATTCTCCTGCCTCCGCCTCCTGAGTAGCTGGG ACTACAGGCCCGTGCCTCTGCGCCCAGCTAATTTTTTGTATTTTTTAGTAGAGACGGGGT TTCACCGTGTTAGCCAGGATGGTTTCGATCTCCTGACCTCGTGATCTGCCCTCCTCGGCC TCCCTAAGTGCTGGGATTACAGGCATGAGCTGCCGCACTCTGCCCAGGTTCTTATTTTTA ATAGAAAAGAGAACAGGGAAGGAACACAGGTCAGTGTGAGGAAGGGGGTCATGGTATACA CAGAGGTGGACTGTTTCTCTACCTCCTCACATTGTGCTAACAGGGACACAGACAGATTCA GAGGCCCTTGCAAAAAGAGAAGCCAGAGTCCCCTAAGACACATAGGGGAGGCGTGAGGAA ATCCTGCATCTCAGTCGCACACAAGGCAGCTGTGCATCTCAGTCGCACACAAGGCAGCTG TCTCAGGCTTTACAAGCTGTGAGACTCAGACCCCTGGGCACTGTCGCTCCACTCAGCCTT AGAGTACCTCCCTCCTTTTCCACCTGAGCTCTTTTTCCTCCATATCACAGCAGCCACCAC AGCTCCAGAGACCACAGATCCAAGGAGAACCAGCCAGCAATGATGCCCACGATGGGGTAT GTGGGCCTGGAAACCCGCTTCCATCTCAAGTGACGGCCTCGGGGTACCCCTCATGTTGAC ATGCACGTGTATCTCTGCTTCTTTCAGAGGGCCCACACAACTGCCCATTTTGGAAGTTCA TCCCTGAAGCCTGTTTCCAGACTCGGGCCGGGTATGCCCTCCATCTGCTGCAGCCATGGA ATCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005516 |
| Insert Size | 1780 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005516.4, NP_005507.3 |
| RefSeq Size | 1701 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 3133 |
| UniProt ID | P13747 |
| Domains | MHC_I, ig, IGc1 |
| Protein Families | Transmembrane |
| Protein Pathways | Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis |
| Gene Summary | HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205939 | HLA (Myc-DDK-tagged)-Human major histocompatibility complex, class I, E (HLA-E) |
CNY 3,656.00 |
|
| RC205939L1 | Lenti ORF clone of Human major histocompatibility complex, class I, E (HLA-E), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205939L2 | Lenti ORF clone of Human major histocompatibility complex, class I, E (HLA-E), mGFP tagged |
CNY 6,056.00 |
|
| RC205939L3 | Lenti ORF clone of Human major histocompatibility complex, class I, E (HLA-E), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205939L4 | Lenti ORF clone of Human major histocompatibility complex, class I, E (HLA-E), mGFP tagged |
CNY 6,056.00 |
|
| RG205939 | HLA (tGFP-tagged) - Human major histocompatibility complex, class I, E (HLA-E) |
CNY 5,256.00 |
|
| SC324555 | HLA (untagged)-Human major histocompatibility complex, class I, E (HLA-E) |
CNY 3,656.00 |
