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FLIP (CFLAR) (NM_003879) Human Untagged Clone
CAT＃: SC127121
CFLAR (untagged)-Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 1
CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; cFLIP; CLARP; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; MRIT |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127121 sequence for NM_003879 edited (data generated by NextGen Sequencing)
ATGTCTGCTGAAGTCATCCATCAGGTTGAAGAAGCACTTGATACAGATGAGAAGGAGATG CTGCTCTTTTTGTGCCGGGATGTTGCTATAGATGTGGTTCCACCTAATGTCAGGGACCTT CTGGATATTTTACGGGAAAGAGGTAAGCTGTCTGTCGGGGACTTGGCTGAACTGCTCTAC AGAGTGAGGCGATTTGACCTGCTCAAACGTATCTTGAAGATGGACAGAAAAGCTGTGGAG ACCCACCTGCTCAGGAACCCTCACCTTGTTTCGGACTATAGAGTGCTGATGGCAGAGATT GGTGAGGATTTGGATAAATCTGATGTGTCCTCATTAATTTTCCTCATGAAGGATTACATG GGCCGAGGCAAGATAAGCAAGGAGAAGAGTTTCTTGGACCTTGTGGTTGAGTTGGAGAAA CTAAATCTGGTTGCCCCAGATCAACTGGATTTATTAGAAAAATGCCTAAAGAACATCCAC AGAATAGACCTGAAGACAAAAATCCAGAAGTACAAGCAGTCTGTTCAAGGAGCAGGGACA AGTTACAGGAATGTTCTCCAAGCAGCAATCCAAAAGAGTCTCAAGGATCCTTCAAATAAC TTCAGGCTCCATAATGGGAGAAGTAAAGAACAAAGACTTAAGGAACAGCTTGGCGCTCAA CAAGAACCAGTGAAGAAATCCATTCAGGAATCAGAAGCTTTTTTGCCTCAGAGCATACCT GAAGAGAGATACAAGATGAAGAGCAAGCCCCTAGGAATCTGCCTGATAATCGATTGCATT GGCAATGAGACAGAGCTTCTTCGAGACACCTTCACTTCCCTGGGCTATGAAGTCCAGAAA TTCTTGCATCTCAGTATGCATGGTATATCCCAGATTCTTGGCCAATTTGCCTGTATGCCC GAGCACCGAGACTACGACAGCTTTGTGTGTGTCCTGGTGAGCCGAGGAGGCTCCCAGAGT GTGTATGGTGTGGATCAGACTCACTCAGGGCTCCCCCTGCATCACATCAGGAGGATGTTC ATGGGAGATTCATGCCCTTATCTAGCAGGGAAGCCAAAGATGTTTTTTATTCAGAACTAT GTGGTGTCAGAGGGCCAGCTGGAGGACAGCAGCCTCTTGGAGGTGGATGGGCCAGCGATG AAGAATGTGGAATTCAAGGCTCAGAAGCGAGGGCTGTGCACAGTTCACCGAGAAGCTGAC TTCTTCTGGAGCCTGTGTACTGCGGACATGTCCCTGCTGGAGCAGTCTCACAGCTCACCA TCCCTGTACCTGCAGTGCCTCTCCCAGAAACTGAGACAAGAAAGAAAACGCCCACTCCTG GATCTTCACATTGAACTCAATGGCTACATGTATGATTGGAACAGCAGAGTTTCTGCCAAG GAGAAATATTATGTCTGGCTGCAGCACACTCTGAGAAAGAAACTTATCCTCTCCTACACA TAA Clone variation with respect to NM_003879.5 >OriGene 5' read for NM_003879 unedited
GGGCACGTACACTATATTTGTAATACGACTCACTATAGGGCGGCCGCGCATAGCAGAAAC AGCGAGCTTGCAGCCTCACCGACGAGTCTCACTAAAAGGACTCCCGGAGCTAGGGGTGGG GACTCGGCCTCACACAGTGAGTGCCGGCTATTGGACTTTTGTCCAGTGACAGCTGAGACA ACAAGGACCACGGGAGGAGGTGTAGGAGAGAAGCGCCGCGAACAGCGATCGCCCAGCACC AAGTCCGCTTCCAGGCTTTCGGTTTCTTTGCCTCCATCTTGGGTGCGCCTTCCCGGCGTC TAGGGGAGCGAAGGCTGAGGTGGCAGCGGCAGGAGAGTCCGGCCGCGACAGGACGAACTC CCCCACTGGAAAGGATTCTGAAAGAAATGAAGTCAGCCCTCAGAAATGAAGTTGACTGCC TGCTGGCTTTCTGTTGACTGGCCCGGAGCTGTACTGCAAGACCCTTGTGAGCTTCCCTAG TCTAAGAGTAGGATGTCTGCTGAAGTCATCCATCAGGTTGAAGAAGCACTTGATACAGAT GAGAAGGAGATGCTGCTCTTTTTGTGCCGGGATGTTGCTATAGATGTGGTTCCACCTAAT GTCAGGGACCTTCTGGGATATTTACGGGGAAAGAGGTAAGCTGTCTGTCGGGGACTTGGC TGAACTGCTCTACAGAGTGAGGCGAATTGGCCTGCTCAAACGTATCTTGGAACATGGACA GAAAGCTGTGGAGACCCACCTTGCTCGGAACCCTCACCTTGGTTTCGGACTATTAAATGC TGGATGGCAAGATTGGTGAGGGATTTGGATAAACCTGAGGTGTCCCCATAAATTTTCCTC ATGAAGGATTCAATGGGCCGGGGCAAGATAACCAGGAAAAAAATT >OriGene 3' read for NM_003879 unedited
NGGTATTACTATGNACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTAAAG AGAGGGATCTTGCTATATTGCCCAGGTTGGTATGCAGTGGCTATTCGTAGGCACAATCAC AGCATGCTATGACCCTGAACTCCTGGGTTCAAAAGATCCTCCCACCTCAGCCTCCCAAGT AACTGGGAATACAGGTACCCACACCCACACCCAGCTAATTTTTGTATTTTTACTAGGGAC AGCGTTTACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTGAAGTGATCTGCCCTCC TTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTACGCCCAGCCTTTTGGTTTC TTATGTGTAGGAGAGGATAAGTTTCTTTCTCAGAGTGTGCTGCAGCCAGACATAATATTT CTCCTTGGCAGAAACTCTGCTGTTCCAATCATACATGTAGCCATTGAGTTCAATGTGAAG ATCCAGGAGTGGGCGTTTTCTTTCTTGTCTCAGTTTCTGGGAGAGGCACTGCAGGTACAG GGATGGTGAGCTGTGAGACTGCTCCAGCAGGGACATGTCCGCAGTACACAGGCTCCAGAA GAAGTCAGCTTCTCGGTGAACTGTGCACAGCCCCTCGCTTCTGAGCCTTGAATTCCACAT TTCTTCATCGCTGATCGCATCCACCTTCAAGAGGCTGCTGTCCTCCAGCTGGGCCTCTGA CACCACATAGTTCTGGATAAAAACATCTTTGGCTTCCCTGCTTGAAAAGGGCATGAATTC TCCCTGAACATCCTCCTGAAGTGATGCAGGGGGAGCCCTTGATGAGTCTGGTCCCACCAT ACACACTCTGGGGAGCCTCTTTGGGCTACCAGGACACCACCAAAGCTGCGGTAATACTCG GTGCTCGGGCATACCGGCAAATTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003879 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003879.3, NP_003870.3 |
| RefSeq Size | 2243 bp |
| RefSeq ORF | 1443 bp |
| Locus ID | 8837 |
| UniProt ID | O15519 |
| Domains | DED, CASc, ICE_p20 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Apoptosis |
| Gene Summary | The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (1) represents use of an alternate first exon and encodes the longest protein isoform (1). Variants 1 and 2 both encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Suppression of cFLIP by Lupeol, a Dietary Triterpene, Is Sufficient to Overcome Resistance to TRAIL-Mediated Apoptosis in Chemoresistant Human Pancreatic Cancer Cells
,Imtiyaz Murtaza, Mohammad Saleem, Vaqar Mustafa Adhami, Bilal Bin Hafeez, and Hasan Mukhtar,
Cancer Res., Feb 2009; 69: 1156 - 1165.
[CFLAR]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200608 | CFLAR (Myc-DDK-tagged)-Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 1 |
CNY 5,488.00 |
|
| RC200608L1 | Lenti ORF clone of Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC200608L2 | Lenti ORF clone of Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200608L3 | Lenti ORF clone of Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC200608L4 | Lenti ORF clone of Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG200608 | CFLAR (tGFP-tagged) - Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 1 |
CNY 7,088.00 |
