United States
Germany
Japan
United Kingdom
China
HLAF (HLA-F) (NM_018950) Human Untagged Clone
CAT＃: SC127080
HLA (untagged)-Human major histocompatibility complex, class I, F (HLA-F), transcript variant 2
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDA12; HLA-5.4; HLA-CDA12; HLAF |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_018950, the custom clone sequence may differ by one or more nucleotides
ATGGCGCCCCGAAGCCTCCTCCTGCTGCTCTCAGGGGCCCTGGCCCTGACCGATACTTGGGCGGGCTCCC ACTCCTTGAGGTATTTCAGCACCGCTGTGTCGCGGCCCGGCCGCGGGGAGCCCCGCTACATCGCCGTGGA GTACGTAGACGACACGCAATTCCTGCGGTTCGACAGCGACGCCGCGATTCCGAGGATGGAGCCGCGGGAG CCGTGGGTGGAGCAAGAGGGGCCGCAGTATTGGGAGTGGACCACAGGGTACGCCAAGGCCAACGCACAGA CTGACCGAGTGGCCCTGAGGAACCTGCTCCGCCGCTACAACCAGAGCGAGGCTGGGTCTCACACCCTCCA GGGAATGAATGGCTGCGACATGGGGCCCGACGGACGCCTCCTCCGCGGGTATCACCAGCACGCGTACGAC GGCAAGGATTACATCTCCCTGAACGAGGACCTGCGCTCCTGGACCGCGGCGGACACCGTGGCTCAGATCA CCCAGCGCTTCTATGAGGCAGAGGAATATGCAGAGGAGTTCAGGACCTACCTGGAGGGCGAGTGCCTGGA GTTGCTCCGCAGATACTTGGAGAATGGGAAGGAGACGCTACAGCGCGCAGATCCTCCAAAGGCACACGTT GCCCACCACCCCATCTCTGACCATGAGGCCACCCTGAGGTGCTGGGCCCTGGGCTTCTACCCTGCGGAGA TCACGCTGACCTGGCAGCGGGATGGGGAGGAACAGACCCAGGACACAGAGCTTGTGGAGACCAGGCCTGC AGGGGATGGAACCTTCCAGAAGTGGGCCGCTGTGGTGGTGCCTCCTGGAGAGGAACAGAGATACACATGC CATGTGCAGCACGAGGGGCTGCCCCAGCCCCTCATCCTGAGATGGGAGCAGTCTCCCCAGCCCACCATCC CCATCGTGGGCATCGTTGCTGGCCTTGTTGTCCTTGGAGCTGTGGTCACTGGAGCTGTGGTCGCTGCTGT GATGTGGAGGAAGAAGAGCTCAGATAGAAACAGAGGGAGCTACTCTCAGGCTGCAGTGTGA >OriGene 5' read for NM_018950 unedited
GTCAACTTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGTCATGGCGC CCCGAAGCCTCCTCCTGCTGCTCTCAGGGCCCTGGCCCTGACCGATACTTGGGCGGGCTC CCACTCCTTGAGGTATTTCAGCACCGCTGTGTCGCGGCCCGGCCGCGGGGAGCCCCGCTA CATCGCCGTGGAGTACGTAGACGACACGCAATTCCTGCGGTTCGACAGCGACGCCGCGAT TCCGAGGATGGAGCCGCGGGAGCCGTGGGTGGAGCAAGAGGGGCCGCAGTATTGGGAGTG GACCACAGGGTACGCCAAGGCCAACGCACAGACTGACCGAGTGGCCCTGAGGAACCTGCT CCGCCGCTACAACCAGAGCGAGGCTGGGTCTCACACCCTCCAGGGAATGAATGGCTGCGA CATGGGGCCCGACGGACGCCTCCTCCGCGGGTATCACCAGCACGCGTACGACGGCAAGGA TTACATCTCCCTGAACGAGGACCTGCGCTCCTGGACCGCGGCGGACACCGTGGCTCAGAT CACCCAGCGCTTCTATGAGGCAGAGGAATATGCAGAGGAGTTCAGGACCTACCTGGAGGG CGAGTGCCTGGAGTTGCTCCGCAGATACTTGGAGAATGGGAAGGAGACGCTACAGCGCGC AGATCCTCCAAAGGCACACGTTGCCCACCACCCCATCTCTGACCATGAGGCCACCCTGAG GTGCTGGGCCCTGGGCTTCTACCCTGCGGAGATCACGCTGACCTGGCAGCGGGATGGGGA GGAACAGACCCAGGACACAGAGCTTGTGGAGACCAGGCCTGCAGGGGATGGAACCTTCCA GAATGGGCCGCTGTGGTGGTGGCTTCTGGAGAGGACAGAGATCACTGCCTGTGCACACGA GGGCTGCCCAGCCCTATNCTGGATGGGACAGTCTCCCAGCCACATCCCATGTGGGCTTGT >OriGene 3' read for NM_018950 unedited
CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTATAAAAAGAATATTTTTATTCTT TATTTTTAAAATTTATGTATGTTCGTGAGGCACAAGTGCAATTCTGCTACATTGATACCT TGCTTCTCAGTCCCACACAAGGAAGCTGTCTCACACTGCAGCCTGAGAGTAGCTCCCTCT GTTTCTATCTGAGCTCTTCTTCCTCCACATCACAGCAGCGACCACAGCTCCAGTGACCAC AGCTCCAAGGACAACAAGGCCAGCAACGATGCCCACGATGGGGATGGTGGGCTGGGGAGA CTGCTCCCATCTCAGGATGAGGGGCTGGGGCAGCCCCTCGTGCTGCACATGGCATGTGTA TCTCTGTTCCTCTCCAGAAGGCACCACCACAGCGGCCCACTTCTGGAAGGTTCCATCCCC TGCAGGCCTGGTCTCCACAAGCTCTGTGTCCTGAGTCTGTTCCTCCCCATCCCGCTGCCA GGTCAGCGTGATCTCCGCAGGGTAGAAGCCCAGGGCCCAGCACCTCAAGGTGGCCTCATG GTCAGAGATGGGGTGGTGGGCAACGTGTGCCCTTGAGGATCTGCGCGCTGTAACGTCTCC CTTCCATTCTCCAAGTATCTGCGGAGCAACTCCAGGCACTCGCCCTCCAGTAAGTCCTGA ACTCCTCTGCTTATTTCTCTGCCTCATAGAAACCGCTGGTGATCTGAGCCACGGTGTCCG CCGCGGTCCAGGAGCGCAGTCCTCGTCAAGGAATGTATCCTTGCCGCGTACCGTGCTGTG ATACCCGCGAGGAGCGTTCGTGGGCCCATGTGCACCATTCTCCCTGGAGGTGGAAACCAC CCCCTTCTGTTGTACCGCGGACAGTTCCTAGGCCCTCGGACATTGGCGTGGCCTTCGTAC CTGGGGCCCTCCAAATGGGCCCCTTGGCTCCACAGTTCCGGTTCCTTCGGATCGCGGTGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018950 |
| Insert Size | 1140 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018950.1, NP_061823.1 |
| RefSeq Size | 1188 bp |
| RefSeq ORF | 1089 bp |
| Locus ID | 3134 |
| UniProt ID | P30511 |
| Domains | MHC_I, ig, IGc1 |
| Protein Families | Transmembrane |
| Protein Pathways | Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Type I diabetes mellitus, Viral myocarditis |
| Gene Summary | This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate 3' exon, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217084 | HLA (Myc-DDK-tagged)-Human major histocompatibility complex, class I, F (HLA-F), transcript variant 2 |
CNY 5,856.00 |
|
| RC217084L3 | Lenti-ORF clone of HLA (Myc-DDK-tagged)-Human major histocompatibility complex, class I, F (HLA-F), transcript variant 2 |
CNY 5,890.00 |
|
| RC217084L4 | Lenti-ORF clone of HLA (mGFP-tagged)-Human major histocompatibility complex, class I, F (HLA-F), transcript variant 2 |
CNY 5,890.00 |
|
| RG217084 | HLA (tGFP-tagged) - Human major histocompatibility complex, class I, F (HLA-F), transcript variant 2 |
CNY 7,456.00 |
