GGA2 (NM_138640) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ADP-ribosylation factor binding protein 2; FLJ20966; golgi associated, gamma adaptin ear containing, ARF binding protein 2; KIAA1080; VEAR; VEAR, FLJ20966, KIAA1080; VHS domain and ear domain-containing protein
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_138640, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGACCGCGGTGGCGGCGGCTGTGGCGGGAACCGAGTCGGCCCAGGGTCCCCCGGGCCCGGCAG CGTCGCTGGAGCTGTGGCTGAACAAAGCCACAGACCCAAGCATGTCGGAACAGGATTGGTCAGCTATCCA GAATTTCTGTGAGCAGGTGAACACTGACCCCAATGGCCCCACACATGCGCCCTGGCTACTGGCCCACAAG ATCCAGTCTCCGCAAGAGAAGGAAGCTCTTTATGCCTTAACGGTGCTGGAGATGTGCATGAACCACTGTG GGGAGAAGTTCCACAGCGAGGTGGCCAAATTTCGTTTCCTGAACGAACTGATCAAAGTGTTGTCCCCAAA GTACCTGGGGTCCTGGGCCACAGGAAAAGTTAAAGGAAGAGTCATTGAAATACTCTTCAGTTGGACAGTC TGGTTTCCGGAAGACATCAAGATTCGAGACGCTTATCAGATGCTGAAGAAACAAGGAATTATAAAACAAG ACCCTAAACTACCAGTGGATAAAATCTTACCCCCACCATCTCCCTGGCCCAAGAGCTCCATCTTTGATGC TGATGAAGAAAAGTCCAAGCTCTTTCTCAGTGCCAGTGAGCCCGGCCCTATCCATTTTCCATCCACCATG AACTCCCCAAACCGTTATTCCTTGGACATCAGTATCTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_138640 unedited GGAAGCGTCAAATTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCA GGACGCTACAGCGGCTGAAGAGGCAGTGGCGCCCGCGGCCGCAGCGTCGGGGCTGGAGCG ATGGCGGCGACCGCGGTGGCGGCGGCTGTGGCGGGAACCGAGTCGGCCCAGGGTCCCCCG GGCCCGGCAGCGTCGCTGGAGCTGTGGCTCAACAAAGCCACAGACCCAAGCATGTCGGAA CAGGATTGGTCAGCTATCCAGAATTTCTGTGAGCAGGTGAACACTGACCCCAATGGCCCC ACACATGCGCCCTGGCTACTGGCCCACAAGATCCAGTCTCCGCAAGAGAAGGAAGCTCTT TATGCCTTAACGGTGCTGGAGATGTGCATGAACCACTGTGGGGAGAAGTTCCACAGCGAG GTGGCCAAATTTCGTTTCCTGAACGAACTGATCAAAGTGTTGTCCCCAAAGTACCTGGGG TCCTGGGCCACAGGAAAAGTTAAAGGAAGAGTCATTGAAATACTCTTCAGTTGGACAGTC TGGTTTCCGGAAGACATCAAGATTCGAGACGCTTATCAGATGCTGAAGAAACAAGGAATT ATAAAACAAGACCCTAAACTACCAGTGGATAAAATCTTACCCCCACCATCTCCCTGGCCC AAGAGCTCCATCTTTGATGCTGATGAAGAAAAGTCCAAGCTTCTGACAAGGCTTCTAAAG AGCAACCACCCCGAGGACCTTCAGGCTGCAAACCGGTTAATCAAGAATTTGGTCAAGGAG GAACAAGAAAAATCGGAGAAGGTGTCCAAGAAGGTCAGTGCGGTGNGAGAAGTGCGAAGC CATGTGAAGGTGCTGCNAGAGATGCTGAGCATGTACCGCAGGCCAGGGCAGGCCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_138640 unedited TTGGCCGCGGCCGCAATCTAGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTAAAATATCAGATTTTGGAATTAAAAGCTCCAAACAA GTTTTGCAGTTTCAAAGGGAAAAACCCACCCAGCCAGTTCAGTTGGTTGGACTTAAAAGC CCTCCGCCTAGCCATTTAAGCCAGGCTAAGGTTCCTTTTCCCACCCATCAGGCCAAGCAG GACTTGGCAAACATACACATTCAAGTTCCTAACACACAGTAGGGGCTAAGGGGGAATTGA TTATAAACTTGAATTTTTCCATCAACAAATATCCACCTTTCCTGTCCAGCTTGCCTAAAA TTTTAAGGTTTTTTTTTTTTTGAGGCAGCTAAGCTTCTACATCCTTCATGAAGTTTCCTT TACTTTTCGACAAAAAACAGTTCCCTTTAGGCCTATCATTTTTAAAGGCAACTGGTTTTC CATTTTCCACTTACTCAACACTCCACAAAGAACACTTGCTACATGGTATTTATTCAAGGA CTGGATATTTAGTCACCACCTCAATCACTGCTAAGCTCTCTTCATGGCTATCTGTACCAG TTTCCCATTTTAAACTGGGACGTCAACTGGCAAGGGCTGGTTGTTGGCCAATTCATCATG ACTGACATGGTGGGTCACAAAGAAGCCTCCATAAAATGCTGTGAAGGAAAAGGATGAGGA TGGAAATGAGGATCCTTGAAAAGCCCAATACTGGTTGCCCAACCATGGACCTGAGGCCTG AATAAGGCCTTGGAACTGTTTTCCCTTTTGCCAAGAACTTTGTCCAGGGGCGATTTAAAG GCCAAGGGGAGAAGCAATGATTGGCCCCCAGGGGGGGCCTCAGGTTTATGGCAGCCACTC CCTGGAGACGGGGCACAAGCATCCTATTAAAAAACTTCCACAAACCACACAAAACTTTGG TTATGAAAG
Restriction Sites	NotI-NotI
ACCN	NM_138640
Insert Size	3630 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_138640.1, NP_619581.1
RefSeq Size	5078 bp
RefSeq ORF	669 bp
Locus ID	23062
Domains	GAT, Alpha_adaptinC2
Protein Pathways	Lysosome
Gene Summary	This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) contains an additional exon between exons 6 and 7 and lacks a downstream sequence segment, as compared to the longer transcript variant 1. This variant encodes isoform 2 which includes an early in-frame stop codon, resulting in a truncated protein.

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