CERK (NM_182661) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ceramide kinase; dA59H18.2; dA59H18.3; DKFZp434E0211; FLJ21430; FLJ23239; hCERK; KIAA1646; lipid kinase LK4; LK4; LK4, hCERK, FLJ21430, FLJ23239, KIAA1646, MGC131878, dA59H18.2, dA59H18.3, DKFZp434E0211; MGC131878; OTTHUMP00000028554
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_182661, the custom clone sequence may differ by one or more nucleotides ATGCAACGTGGCCCTGCTTCAGGTGATCCGCGGGAGGGGCCTCCACGCCAGCGCCGGGAAGGCTGCTGGG GCCTCCACACCTGCCTCATCACGGCGGCGAGGCTACGACAATCCGGCTGGGAGCATGACCTTGGCGTCTG TTCTGGGAGCACGGATGATAAGCTCTGGAAGCTGGCAGTGTGTAAAGCACTGGCAAGTTTGTTACTGTTA AAATGTCAAATACCAATGCTTTATATCGACGCGAAGTGCTTAACACAGCCGGGCTTGGGGGCAGTCAGGA GGAAGCTGGCCATCCGTGGAGGAGGGGCCGGTCCTGGACTCCCGCAGGACTCCTCTGAGGCAGGGCCTGA AGTCTGTACACGTGGTCCAGATTTGTCCTTGTCTTTTCTTCACACTGAGTTCTCTATATTTATTGAACAT CTTGTCCTTTTAAGCCAGAGTAGTGTAAACTGCGTCTCGGATGTCTGTCTTTTGCCTCGAAGCCACGATG GATCGCTGGTTTCCTCTGCAGCGCGAGGGCTCCGGCGACCAGAGGATTCTTCCCGGAAGGCATTCCTGCC GCGCTCCCCGGGGCACCCCTCAATTGTGTACTACGTCCTTGTTTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_182661 unedited ATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCCTGGAACTGCGACGGGGAGG TCCTGCACAGCCCTGCCATCGAGGTCAGAGTCCACTGCCAGCTGGTTCGACTCTTTGCAC GAGGAATTGAAGAGAATCCGAAGCCAGACTCACACAGCTGAGAAGCCGGCGTCCTGCTCA CAAACTGGGAAAGTGTGAAAACTATTTAAGATAATTATTACAGACCAATTATGTTGATAT ATACATTTAAATGTAGAAATTTATTTTTGATAGTTAAATCTTGATTTTAGAAGAAAACCC TTTTGTCAACAATTTTGTGTACATATTTGGCATTTTCAGTTCTGTACGCATCTGCGGGTT GCAGCCCACGCCGCTTACTCTCAGCGGATGCAGCTGCTCACTTGGGGGCACTGGCCTCTT AGGTTTTAACGATGTCAACAGTGTAGTTTAGAAAATGGCCCGTTAGTGGCTCTATTGCAA TAATGTTAGGGACATTATATGATTTCCACGCAGGTCACACCATCTGGGCCTGAGGTAGCA GTGGGTCACTTTGATCCACTTTGCAGGACTTATTCTGTAACGGTTTGTGGCCAAGTTTTG GGAAGTGGTTGATTCTCTTTGCCTTCATTTCACCTTCCTCTTCGTTTACGGTTAGGACAT NCGCTGCTTGATCCTTACAATACTGTGCAACTGCAATGCACGTGGCCCTGCTTCAGGTGA ATCGCGGGAGGGGGCCTCCGCCACGCCGGNAGGCTGCTGGGGCCTNCACACTGCCTATCA CGGGGCGAGGCTCGACATNCGCTGGAGATACCTGGGGGTCTGTTCTGAGCACGAGATACT CTGAACTGGGCATGGTAAGCTGGCAAGTTTGTCTGTTTAATGTAATACATGCTAATNNAC CCAGGTAAACACGGCTTTGGGCATAGAAGATGCTTCCTTGGGGGGGGGGGGGGCCTGTCT CCAACCCTTAGGGGGGGAGTTTCACGGGGCAAATTTTGTTCTCTAACGTTTTTA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_182661 unedited GATTACTTTTTTTCGTGGTCTTTCTCAAACATTTNCANANCAAATAATGCCATTTTCTAA TCTTGAAACCAGCAAGATTAGAAAGAGAATGAGGCCATCCTTTCACTCAGACTTTCCCAG TTACTCAAGATTGTATTCAGGAACGAAGTCAAACTCGGCCTCACTTCTTAATTAGAATAA ACTAGAATCAGGTCCATTGCATAACTGCTAATTGTTTTGCACCAGGGAGCCACAAAGGAA GCTAAAACTAGCCTTTCTAAGTTATCCTAAGTTTTTACTATCTAACCAGCACTTCTGATT TTTTTTCCCTTAAAACTCTAAAGTACAGGTGTTGTATGTCTGCCGCATCTGAAAGGCTGC CCCGGAAATCAGAGCCTATCTAGCGGGAATGTCTGGGCAGTCACCCCCATCCTGCGCGCA GATAGCAAAGGGCTGCTGGCAGGTGTCAGACACACGGTACCGCAACAGCAGTCCCGAGGT CTGAGATTCTGAATGTCAGCAGGTGGCTGGAGAGTATCACCAGAGCCACTCAGGAACCAG CCATGCGGAATGGCTGGGTTTCTTATTGAACATGAAATGCTTCCTCTTTGGCCTGGACTG TTTTGCTAAAATTGTTAGTGGGTTTTGGGGCAGGCCAGTGTATGGGAAACTTTTTTTTTT GTTGGGGGGGGGGCGATGGAGTCTCCCTCTGTTGCCCAGGCCTGGAGTGCAGTGGCGCAA TCTTGGCTCACCGCAACCTCCACCTCTTTCTTGGNGTCAAGCGATTCTCCTGCCTCAGCA TNCCTAGTAGCTGAGAATACAGGGCGTGCGCCCAACAGACTGGGCTAAGNTNGTGTATTT TTAGTAGAGGACGAGGTTTCACCATGTTTGGGCAAGCTGATCTCAACTCCTGACCTTTAG TGATCCACCGCCTCGGCTTCCAAGTGCTGGGATNCAGGCGTGAGCACCGGCCCAGCGGGA ACATTACAGGGACATAGCCTANAGAAACTTTGGGAACTTTGGAGGACCAATCN
Restriction Sites	NotI-NotI
ACCN	NM_182661
Insert Size	2850 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_182661.1, NP_872602.1
RefSeq Size	3040 bp
RefSeq ORF	606 bp
Locus ID	64781
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Sphingolipid metabolism
Gene Summary	CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]

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