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GCDH (NM_013976) Human Untagged Clone
CAT＃: SC126971
GCDH (untagged)-Human glutaryl-CoA dehydrogenase (GCDH), nuclear gene encoding mitochondrial protein, transcript variant 2
CNY 6,840.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACAD5; GCD |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_013976, the custom clone sequence may differ by one or more nucleotides
ATGGCCCTGAGAGGCGTCTCCGTGCGGCTGCTGAGCCGCGGACCCGGCCTGCACGTCCTTCGCACGTGGG TCTCGTCGGCGGCGCAGACCGAGAAAGGCGGGAGAACACAGAGCCAACTGGCTAAGTCCTCGCGTCCCGA GTTTGACTGGCAGGACCCGCTGGTGCTGGAGGAGCAGCTGACCACAGATGAGATCCTCATCAGGGACACC TTCCGCACCTACTGCCAGGAGAGACTCATGCCTCGCATCCTGTTGGCCAATCGCAACGAAGTTTTTCATC GGGAGATCATTTCGGAGATGGGGGAGTTGGGTGTGCTGGGCCCCACCATCAAAGGATATGGCTGTGCTGG GGTTTCGTCTGTGGCCTATGGGCTCCTGGCCCGAGAGCTGGAGCGGGTGGACAGTGGCTACAGGTCGGCG ATGAGTGTCCAGTCCTCCCTCGTCATGCACCCTATCTATGCCTATGGCAGCGAGGAACAGCGGCAGAAGT ACCTGCCCCAGCTGGCCAAGGGGGAGCTCCTGGGCTGCTTCGGGCTCACAGAGCCCAACAGCGGAAGTGA CCCCAGCAGCATGGAGACCAGAGCCCACTACAACTCATCCAACAAGAGCTACACCCTCAATGGGACCAAG ACCTGGATCACGAACTCGCCTATGGCCGATCTGTTTGTAGTGTGGGCTCGGTGTGAAGATGGCTGCATTC GGGGCTTCCTGCTGGAGAAGGGGATGCGGGGTCTCTCGGCCCCCAGGATCCAGGGCAAGTTCTCGCTGCG GGCCTCAGCCACAGGCATGATCATCATGGACGGTGTGGAGGTGCCAGAGGAGAATGTGCTCCCTGGTGCA TCCAGCCTGGGGGGTCCCTTCGGCTGCCTGAACAACGCCCGGTACGGCATCGCGTGGGGCGTGCTTGGAG CTTCGGAGTTCTGCTTGCACACAGCCCGGCAGTACGCCCTCGACAGGATGCAGTTTGGTGTCCCACTGGC CAGGAACCAGCTGATTCAGAAGAAGCTGGCAGACATGCTCACTGAGATTACCCTGGGCCTTCACGCCTGC CTGCAGCTCGGCCGCTTGAAGGACCAGGACAAGGCTGCCCCCGAGATGGTTTCTCTGCTGAAGAGGAATA ACTGTGGGAAAGCCCTGGACATCGCCCGCCAGGCCCGAGACATGCTGGGGGGGAATGGGATTTCTGACGA GTATCACGTGATCCGGCACGCCATGAACCTGGAGGCCGTGAACACCTACGAAGTCGTTCAGATGTGTTCC TTAAAAAGAAGATGGAATTCTCTGTAG >OriGene 5' read for NM_013976 unedited
TGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGGAACCGGGAGGTACTACC AGGTCGCCGTCGTTGCTCCGCTCGCTCTGAGAGAGCATGGCCCTGAGAGGCGTCTCCGTG CGGCTGCTGAGCCGCGGACCCGGCCTGCACGTCCTTCGCACGTGGGTCTCGTCGGCGGCG CAGACCGAGAAAGGCGGGAGAACACAGAGCCAACTGGCTAAGTCCTCGCGTCCCGAGTTT GACTGGCAGGACCCGCTGGTGCTGGAGGAGCAGCTGACCACAGATGAGATCCTCATCAGG GACACCTTCCGCACCTACTGCCAGGAGAGACTCATGCCTCGCATCCTGTTGGCCAATCGC AACGAAGTTTTTCATCGGGAGATCATTTCGGAGATGGGGGAGTTGGGTGTGCTGGGCCCC ACCATCAAAGGATATGGCTGTGCTGGGGTTTCGTCTGTGGCCTATGGGCTCCTGGCCCGA GAGCTGGAGCGGGTGGACAGTGGCTACAGGTCGGCGATGAGTGTCCAGTCCTCCCTCGTC ATGCACCCTATCTATGCCTATGGCAGCGAGGAACAGCGGCAGAAGTACCTGCCCCAGCTG GCCAACGGGGAGCTCCTGGGCTGCTTCGGGCTCACAGAGCCCAACAGCGGGAAGTGACCC CAGCAGCATGGAGACCAGAGCCCACTACAACTCATCCAACAAGAGCTACACCCTCAATGG GACCAAGACCTGGATCACGAACTCGCCTATGGCCGATCTGTTTGTAGTGTGGCCTCCGTG TGAAGATGCCTGCATTCGGNGCTTCCTGCTGGAGAAGGGATGCGGGTCTCTCGCCCCCCA GATCAGNGGCAGTCTCGCTGCGGCCTCAGCACAGGCTGACATATGGACGTGTGGAGTGCC CAGGAGATTGCTCCCTGTGCATCAGCG >OriGene 3' read for NM_013976 unedited
TAGCTTGNACCCGCCCGCTTTCTAGNGATCAGTTTTCCTTTTTTTTTTTTTCAAAGTGCA GGTCTTTATTTTCCTCTCCCTCACTCTGCTCCAACACCCAGCATAAGGCACTACCCCCAG ATGGGAGGGAGGGAGGGCGCACTGTGAACTCAGTGTGAGGGGGTCAGCTGCAGCAGACGG AGTTGCTTCCATGTCACTCTCCTCTCAAGAGAAGCTGCTATTTCAGGGTAAATGGAGTCT GCTCTCATCCATGGTTAAAAGTGGATTGAGACGCTCTACAGAGAATTCCATCTTCTTTTT AAGGAACACATCCGAACGACTTCAGAAGGGAAATTTTGATATTTAAAAATCAGAGTCTCT CACTTCCCACTCCATCCGCCACCTCCCTTTCTAAGCTCAGAGCACAGCGCTCCTACGGTC CAGCCAGGCATCTCTCCAGAAAGGGGCTTGAGAGTTTCGGGCCCCTGATGGAGCGGCTCA CTTGCTGGCCGTGAACGCCTGGATTCCCGTGATAGCTCTCCCAAGGATCAGGGCGTGAAT GCCATGTGTACCTTCGTAGGTGTTCACGGCCTCCAGGTTCATGGCGTGCCGGATCACGTG ATACTCGTCAGAAATCCCATTCCCCCCCAGCATGTCTCGGGCCTGGCGGGCGATGTCCAG GGCTTTCCCACAGTTATTCCTCTTCAGCAGAGAAACCATCTCGGGGGCAGCCTTGTCCTG GTCCTTCAAGCGGCCGAGCTGCAGGCAGGCGTGAAGGCCCAGGTAATCTCAGTGAGCATG TCTGCCAGCTTCTTCTGATCAGCTGGCTCTGGCCAGTGGAACACAACTGCATCTGTCGAG GCGTCTGCCCGGCTGTGTGCAGCATACTCCGAGCTCAGCCACGCCCACCGATGCGACCGG GCGTTGTCAGGACCCGAAGGCCCCCAGCCTGTAGCCCAGGAACCATCCTCTCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_013976 |
| Insert Size | 1830 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013976.2, NP_039663.1 |
| RefSeq Size | 1607 bp |
| RefSeq ORF | 1287 bp |
| Locus ID | 2639 |
| UniProt ID | Q92947 |
| Domains | Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N |
| Protein Families | Druggable Genome |
| Protein Pathways | Fatty acid metabolism, Lysine degradation, Metabolic pathways, Tryptophan metabolism |
| Gene Summary | The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Mutations in this gene result in the metabolic disorder glutaric aciduria type 1, which is also known as glutaric acidemia type I. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 12. [provided by RefSeq, Mar 2013] Transcript Variant: This variant (2) lacks a segment that results in a frameshift in the 3' coding region, compared to variant 1. The resulting isoform (b, also known as the short isoform) has a distinct and shorter C-terminus, compared to isoform a. This variant lacks publicly available transcript support but is supported by data in PubMed ID:8541831. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213591 | GCDH (Myc-DDK-tagged)-Human glutaryl-CoA dehydrogenase (GCDH), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 3,656.00 |
|
| RC213591L3 | Lenti-ORF clone of GCDH (Myc-DDK-tagged)-Human glutaryl-CoA dehydrogenase (GCDH), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5,890.00 |
|
| RC213591L4 | Lenti-ORF clone of GCDH (mGFP-tagged)-Human glutaryl-CoA dehydrogenase (GCDH), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5,890.00 |
|
| RG213591 | GCDH (tGFP-tagged) - Human glutaryl-CoA dehydrogenase (GCDH), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 4,370.00 |
