NNT (NM_182977) Human Untagged Clone

CAT#: SC126852

NNT (untagged)-Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 2



  "NM_182977" in other vectors (4)

CNY 7,568.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-NNT Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GCCD4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_182977, the custom clone sequence may differ by one or more nucleotides


ATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTCGTGTCCTCTACTTAGCAATTTGGGGTCCTGTA
AGGGTCTACGTGTGAAGAAGGATTTTTTACGAACATTTTATACTCACCAAGAACTGTGGTGTAAAGCGCC
TGTAAAACCAGGAATTCCATATAAGCAACTGACTGTTGGAGTCCCCAAAGAGATATTCCAAAATGAGAAG
CGAGTGGCATTGTCTCCTGCTGGTGTTCAGAACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAATCGG
GTGCGGGCGAAGCTTCCAAGTTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAATCCAAGGGGCAAA
GGAAGTGCTGGCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGTTAATCCAACATTAGGTGTTCAT
GAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAGTTTTATTTACCCAGCCCAAAATCCAGAGTTGC
TAAATAAACTTTCCCAAAGAAAAACTACAGTTCTGGCAATGGACCAGGTTCCAAGAGTCACAATTGCTCA
GGGATATGATGCGCTAAGCTCCATGGCCAACATTGCGGGTTATAAGGCTGTTGTCCTAGCAGCAAATCAT
TTTGGACGTTTTTTTACTGGTCAGATCACAGCTGCTGGAAAAGTTCCTCCAGCTAAGATTCTGATAGTTG
GTGGTGGTGTTGCTGGGCTTGCTTCTGCAGGCGCAGCAAAGTCGATGGGTGCAATTGTTCGAGGATTTGA
CACAAGAGCTGCAGCTTTGGAACAGTTCAAGTCTCTTGGTGCTGAGCCCTTGGAGGTGGACTTGAAGGAA
TCTGGTGAGGGACAAGGAGGATATGCAAAAGAGATGTCCAAAGAGTTCATTGAAGCTGAAATGAAACTCT
TTGCTCAACAATGCAAGGAGGTAGACATCCTTATCAGCACAGCACTTATTCCAGGTAAAAAAGCTCCAGT
TTTATTTAATAAAGAAATGATTGAGTCAATGAAGGAAGGTTCAGTTGTTGTGGATTTAGCTGCTGAGGCT
GGTGGAAACTTTGAAACCACTAAGCCAGGAGAACTCTACATTCATAAGGGAATTACTCACATAGGCTACA
CAGACCTGCCCAGCCGAATGGCCACTCAGGCCAGCACCCTATATTCCAACAACATCACCAAACTCCTGAA
GGCCATCAGCCCGGACAAAGATAATTTTTATTTTGATGTGAAAGATGACTTTGACTTTGGTACGATGGGT
CATGTCATTAGAGGAACTGTAGTGATGAAAGATGGTAAAGTGATTTTCCCAGCTCCCACACCGAAAAATA
TTCCTCAAGGTGCCCCAGTAAAACAGAAGACAGTGGCTGAGCTGGAAGCTGAAAAAGCAGCTACCATTAC
ACCCTTCAGGAAGACAATGTCAACGGCTTCTGCATATACAGCAGGTCTCACAGGGATACTGGGTTTGGGC
ATTGCGGCTCCCAATCTAGCCTTTTCTCAGATGGTGACCACTTTTGGCTTGGCTGGCATTGTGGGGTATC
ATACCGTCTGGGGAGTGACCCCTGCTCTCCACTCACCACTGATGTCTGTGACAAATGCAATCTCAGGGCT
GACTGCAGTTGGTGGGTTGGCACTGATGGGAGGACATTTGTATCCTTCCACAACTTCTCAGGGCCTTGCT
GCTCTTGCTGCATTCATATCCTCTGTCAACATTGCAGGTGGCTTTCTGGTGACTCAGAGAATGCTGGACA
TGTTCAAGCGTCCCACTGACCCCCCAGAATACAACTACCTGTACCTGCTCCCTGCCGGCACCTTTGTTGG
TGGATATTTAGCTGCCCTCTACAGTGGTTATAACATTGAACAGATCATGTACCTAGGCTCGGGTTTGTGC
TGTGTCGGTGCCTTGGCTGGCCTCTCCACCCAGGGAACAGCACGTCTTGGCAATGCACTGGGCATGATTG
GGGTTGCTGGAGGACTGGCAGCCACCCTCGGAGTCCTAAAACCGGGCCCAGAATTACTAGCTCAGATGTC
TGGAGCGATGGCTTTGGGTGGTACCATTGGATTGACAATTGCCAAACGCATCCAGATTTCTGATTTACCT
CAATTAGTTGCTGCTTTTCACAGTTTAGTGGGTTTGGCAGCTGTACTTACTTGCATAGCTGAGTACATTA
TAGAATATCCACATTTTGCTACGGATGCAGCAGCAAATCTCACCAAGATTGTGGCCTACCTCGGCACTTA
CATTGGTGGCGTCACCTTTAGTGGGTCTCTCATTGCCTATGGAAAATTGCAGGGTCTCCTGAAATCTGCC
CCTCTCCTACTGCCTGGAAGGCACTTACTCAATGCAGGCTTACTGGCTGCTAGTGTGGGCGGGATAATCC
CATTCATGGTGGACCCAAGCTTTACTACTGGCATCACCTGTCTGGGTTCAGTGTCTGCTCTCTCTGCTGT
CATGGGTGTGACTTTGACAGCTGCTATTGGGGGTGCTGACATGCCCGTCGTTATCACTGTGCTGAACAGC
TACTCAGGCTGGGCCCTGTGTGCAGAGGGCTTCCTGCTCAACAACAATCTGCTGACCATCGTGGGTGCAC
TCATAGGCTCGTCTGGTGCTATCCTGTCATACATCATGTGTGTGGCAATGAATCGCTCCCTGGCTAATGT
GATTCTTGGAGGCTATGGCACCACTTCAACAGCTGGTGGAAAACCCATGGAAATTTCTGGCACACATACG
GAAATCAACCTTGACAATGCAATTGACATGATTCGAGAAGCTAATAGCATTATTATTACACCAGGCTATG
GTCTCTGTGCAGCCAAAGCTCAATACCCCATTGCTGATTTGGTAAAGATGCTCACTGAGCAAGGCAAAAA
AGTCAGGTTTGGAATTCACCCAGTTGCAGGCCGAATGCCTGGTCAGCTTAATGTGCTGCTGGCTGAGGCT
GGTGTGCCATATGACATTGTGTTGGAAATGGATGAGATCAACCATGATTTTCCAGATACTGATTTGGTCC
TTGTAATTGGAGCTAATGACACTGTTAATTCAGCAGCTCAAGAAGATCCCAACTCTATTATTGCAGGCAT
GCCAGTCCTTGAGGTCTGGAAATCAAAGCAGGTGATTGTTATGAAGAGGTCTTTGGGTGTTGGCTATGCT
GCAGTGGACAATCCAATCTTCTACAAACCTAACACGGCCATGCTTCTAGGTGATGCCAAGAAAACATGTG
ACGCGCTCCAGGCGAAAGTTAGAGAATCCTATCAGAAGTAA


>OriGene 5' read for NM_182977 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCAGCGCCGCGGG
GCCCAAGCCCGGGTCTGCCAGCGCGACGTCCTCTCGCGGCCCTCAGGGCACAGCCCAAGG
CTGTCAGCCTCCCGGCCCAGTGATTTGCCTTCAAGGAAACTGGGGAGTCAGAAAATTGGG
AACTCATATCAACATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTCGTGTCCTCT
ACTTAGCAATTTGGGGTCCTGTAAGGGTCTACGTGTGAAGAAGGATTTTTTACGAACATT
TTATACTCACCAAGAACTGTGGTGTAAAGCGCCTGTAAAACCAGGAATTCCATATAAGCA
ACTGACTGTTGGAGTCCCCAAAGAGATATTCCAAAATGAGAAGCGAGTGGCATTGTCTCC
TGCTGGTGTTCAGAACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAATCGGGTGCGGG
CGAAGCTTCCAAGTTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAATCCAAGGGGC
AAAGGAAGTGCTGGCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGTTAATCCAAC
ANTTAGGTGTTCATGAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAGTTTTATTT
ACCCAGCCCAAAATCCAGAGTTGCTAAATAAACTTTCCCAAGAAAAACTACAGTTCTGGC
AATGGACCAGGTTCCAAGAGTCACAATTGCTCANGGATATGATGCGCTAAGCTCCATGGC
CACATTGCGGGTTATAAGGGCTGNTGTCCTAGCACANATCATTTTGGNACGTTTTTACTG
GTCAGAAACAGCTGTTGGAAAAGTTCCTCAGCTAGATCTGAAGTGGGGG
>OriGene 3' read for NM_182977 unedited
GGGGNNNNNNAAAATNTTGACTTGNACCGCGGNNCCGCATTCTNANGATCGAGTTTTTTT
TTTTTTTTTTTGCCAACTGATGAACTCTTTTATTAGTCAAAGTTGAATTCCACTCAAGTG
CACTTAATATAGTACTACACATTATAAGTTGATCGAAATTAAAGAGATCTCCAATTAGAA
ATGGGATCTTCAAAAATACTATTACTAATTTTCAAGTTGTATGTTTCATTGGTATTCTAA
TGAAACATATAAAGCAAATGATTTGTTTCCAGCATCTCTGAAAGATTATTTAGTCCTTTA
ATGTATTAAAATAGTAGTGAGAAAATGTGCCCTTGAAATATAGACTCTGAAGTACAATTT
AAAAAATTAAAACTGATTTGATATGATTAATTCAAACTGCGGTTGAATTGCAAGGGGACA
GCTCAGAAGTTCATAAAAGTTTCAAATTAGTCACATTTGTTGAAAAAAAAAACATAAAAT
GCAGGTGCCTGCAATGTTTACATTGTGTCAGATATTTCAGAGCCATGTATAATGTTGTCT
TGAAATCCTAAACACTTAGTTTTTTAAAACATTTCTGGTTACTGAGGAGGCATAAAAAAT
CCACAGGACTACATCTAAAATGAAGGCTGTCTTTCTCCTTTTATAAATACTTTTGATTCC
TAACACAAAAATATGCACAGAAAGACATTTAGAATTTTCAATCCTTTTTAAAAATGAGGG
ATACTGCTTTTGAAAATCTCTCTAACAGTTTTTGCTTGCCTTCTTCANCTCATTTTCTCC
AAGAGCTTGCTAAGATGGCATCACCTGTTACTGAACTTTATTGCCTGGTCCAAACTGCGA
GGTGGCATATAGCTACAGCTGATCTTAAATTACTCTGAAGGATCCTACCTCGCTGGACCC
GACATGTTTCTGGCATACTAAACATGGCGGGTAGTTGTAAAA
Restriction Sites NotI-NotI     
ACCN NM_182977
Insert Size 4180 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_182977.1, NP_892022.1
RefSeq Size 4232 bp
RefSeq ORF 3261 bp
Locus ID 23530
UniProt ID Q13423
Protein Families Transmembrane
Protein Pathways Metabolic pathways, Nicotinate and nicotinamide metabolism
Gene Summary This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. [provided by RefSeq, Sep 2016]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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