NNT (NM_182977) Human Untagged Clone
CAT#: SC126852
NNT (untagged)-Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 2
CNY 7,568.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | GCCD4 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_182977, the custom clone sequence may differ by one or more nucleotides
ATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTCGTGTCCTCTACTTAGCAATTTGGGGTCCTGTA AGGGTCTACGTGTGAAGAAGGATTTTTTACGAACATTTTATACTCACCAAGAACTGTGGTGTAAAGCGCC TGTAAAACCAGGAATTCCATATAAGCAACTGACTGTTGGAGTCCCCAAAGAGATATTCCAAAATGAGAAG CGAGTGGCATTGTCTCCTGCTGGTGTTCAGAACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAATCGG GTGCGGGCGAAGCTTCCAAGTTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAATCCAAGGGGCAAA GGAAGTGCTGGCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGTTAATCCAACATTAGGTGTTCAT GAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAGTTTTATTTACCCAGCCCAAAATCCAGAGTTGC TAAATAAACTTTCCCAAAGAAAAACTACAGTTCTGGCAATGGACCAGGTTCCAAGAGTCACAATTGCTCA GGGATATGATGCGCTAAGCTCCATGGCCAACATTGCGGGTTATAAGGCTGTTGTCCTAGCAGCAAATCAT TTTGGACGTTTTTTTACTGGTCAGATCACAGCTGCTGGAAAAGTTCCTCCAGCTAAGATTCTGATAGTTG GTGGTGGTGTTGCTGGGCTTGCTTCTGCAGGCGCAGCAAAGTCGATGGGTGCAATTGTTCGAGGATTTGA CACAAGAGCTGCAGCTTTGGAACAGTTCAAGTCTCTTGGTGCTGAGCCCTTGGAGGTGGACTTGAAGGAA TCTGGTGAGGGACAAGGAGGATATGCAAAAGAGATGTCCAAAGAGTTCATTGAAGCTGAAATGAAACTCT TTGCTCAACAATGCAAGGAGGTAGACATCCTTATCAGCACAGCACTTATTCCAGGTAAAAAAGCTCCAGT TTTATTTAATAAAGAAATGATTGAGTCAATGAAGGAAGGTTCAGTTGTTGTGGATTTAGCTGCTGAGGCT GGTGGAAACTTTGAAACCACTAAGCCAGGAGAACTCTACATTCATAAGGGAATTACTCACATAGGCTACA CAGACCTGCCCAGCCGAATGGCCACTCAGGCCAGCACCCTATATTCCAACAACATCACCAAACTCCTGAA GGCCATCAGCCCGGACAAAGATAATTTTTATTTTGATGTGAAAGATGACTTTGACTTTGGTACGATGGGT CATGTCATTAGAGGAACTGTAGTGATGAAAGATGGTAAAGTGATTTTCCCAGCTCCCACACCGAAAAATA TTCCTCAAGGTGCCCCAGTAAAACAGAAGACAGTGGCTGAGCTGGAAGCTGAAAAAGCAGCTACCATTAC ACCCTTCAGGAAGACAATGTCAACGGCTTCTGCATATACAGCAGGTCTCACAGGGATACTGGGTTTGGGC ATTGCGGCTCCCAATCTAGCCTTTTCTCAGATGGTGACCACTTTTGGCTTGGCTGGCATTGTGGGGTATC ATACCGTCTGGGGAGTGACCCCTGCTCTCCACTCACCACTGATGTCTGTGACAAATGCAATCTCAGGGCT GACTGCAGTTGGTGGGTTGGCACTGATGGGAGGACATTTGTATCCTTCCACAACTTCTCAGGGCCTTGCT GCTCTTGCTGCATTCATATCCTCTGTCAACATTGCAGGTGGCTTTCTGGTGACTCAGAGAATGCTGGACA TGTTCAAGCGTCCCACTGACCCCCCAGAATACAACTACCTGTACCTGCTCCCTGCCGGCACCTTTGTTGG TGGATATTTAGCTGCCCTCTACAGTGGTTATAACATTGAACAGATCATGTACCTAGGCTCGGGTTTGTGC TGTGTCGGTGCCTTGGCTGGCCTCTCCACCCAGGGAACAGCACGTCTTGGCAATGCACTGGGCATGATTG GGGTTGCTGGAGGACTGGCAGCCACCCTCGGAGTCCTAAAACCGGGCCCAGAATTACTAGCTCAGATGTC TGGAGCGATGGCTTTGGGTGGTACCATTGGATTGACAATTGCCAAACGCATCCAGATTTCTGATTTACCT CAATTAGTTGCTGCTTTTCACAGTTTAGTGGGTTTGGCAGCTGTACTTACTTGCATAGCTGAGTACATTA TAGAATATCCACATTTTGCTACGGATGCAGCAGCAAATCTCACCAAGATTGTGGCCTACCTCGGCACTTA CATTGGTGGCGTCACCTTTAGTGGGTCTCTCATTGCCTATGGAAAATTGCAGGGTCTCCTGAAATCTGCC CCTCTCCTACTGCCTGGAAGGCACTTACTCAATGCAGGCTTACTGGCTGCTAGTGTGGGCGGGATAATCC CATTCATGGTGGACCCAAGCTTTACTACTGGCATCACCTGTCTGGGTTCAGTGTCTGCTCTCTCTGCTGT CATGGGTGTGACTTTGACAGCTGCTATTGGGGGTGCTGACATGCCCGTCGTTATCACTGTGCTGAACAGC TACTCAGGCTGGGCCCTGTGTGCAGAGGGCTTCCTGCTCAACAACAATCTGCTGACCATCGTGGGTGCAC TCATAGGCTCGTCTGGTGCTATCCTGTCATACATCATGTGTGTGGCAATGAATCGCTCCCTGGCTAATGT GATTCTTGGAGGCTATGGCACCACTTCAACAGCTGGTGGAAAACCCATGGAAATTTCTGGCACACATACG GAAATCAACCTTGACAATGCAATTGACATGATTCGAGAAGCTAATAGCATTATTATTACACCAGGCTATG GTCTCTGTGCAGCCAAAGCTCAATACCCCATTGCTGATTTGGTAAAGATGCTCACTGAGCAAGGCAAAAA AGTCAGGTTTGGAATTCACCCAGTTGCAGGCCGAATGCCTGGTCAGCTTAATGTGCTGCTGGCTGAGGCT GGTGTGCCATATGACATTGTGTTGGAAATGGATGAGATCAACCATGATTTTCCAGATACTGATTTGGTCC TTGTAATTGGAGCTAATGACACTGTTAATTCAGCAGCTCAAGAAGATCCCAACTCTATTATTGCAGGCAT GCCAGTCCTTGAGGTCTGGAAATCAAAGCAGGTGATTGTTATGAAGAGGTCTTTGGGTGTTGGCTATGCT GCAGTGGACAATCCAATCTTCTACAAACCTAACACGGCCATGCTTCTAGGTGATGCCAAGAAAACATGTG ACGCGCTCCAGGCGAAAGTTAGAGAATCCTATCAGAAGTAA >OriGene 5' read for NM_182977 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCAGCGCCGCGGG GCCCAAGCCCGGGTCTGCCAGCGCGACGTCCTCTCGCGGCCCTCAGGGCACAGCCCAAGG CTGTCAGCCTCCCGGCCCAGTGATTTGCCTTCAAGGAAACTGGGGAGTCAGAAAATTGGG AACTCATATCAACATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTCGTGTCCTCT ACTTAGCAATTTGGGGTCCTGTAAGGGTCTACGTGTGAAGAAGGATTTTTTACGAACATT TTATACTCACCAAGAACTGTGGTGTAAAGCGCCTGTAAAACCAGGAATTCCATATAAGCA ACTGACTGTTGGAGTCCCCAAAGAGATATTCCAAAATGAGAAGCGAGTGGCATTGTCTCC TGCTGGTGTTCAGAACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAATCGGGTGCGGG CGAAGCTTCCAAGTTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAATCCAAGGGGC AAAGGAAGTGCTGGCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGTTAATCCAAC ANTTAGGTGTTCATGAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAGTTTTATTT ACCCAGCCCAAAATCCAGAGTTGCTAAATAAACTTTCCCAAGAAAAACTACAGTTCTGGC AATGGACCAGGTTCCAAGAGTCACAATTGCTCANGGATATGATGCGCTAAGCTCCATGGC CACATTGCGGGTTATAAGGGCTGNTGTCCTAGCACANATCATTTTGGNACGTTTTTACTG GTCAGAAACAGCTGTTGGAAAAGTTCCTCAGCTAGATCTGAAGTGGGGG >OriGene 3' read for NM_182977 unedited
GGGGNNNNNNAAAATNTTGACTTGNACCGCGGNNCCGCATTCTNANGATCGAGTTTTTTT TTTTTTTTTTTGCCAACTGATGAACTCTTTTATTAGTCAAAGTTGAATTCCACTCAAGTG CACTTAATATAGTACTACACATTATAAGTTGATCGAAATTAAAGAGATCTCCAATTAGAA ATGGGATCTTCAAAAATACTATTACTAATTTTCAAGTTGTATGTTTCATTGGTATTCTAA TGAAACATATAAAGCAAATGATTTGTTTCCAGCATCTCTGAAAGATTATTTAGTCCTTTA ATGTATTAAAATAGTAGTGAGAAAATGTGCCCTTGAAATATAGACTCTGAAGTACAATTT AAAAAATTAAAACTGATTTGATATGATTAATTCAAACTGCGGTTGAATTGCAAGGGGACA GCTCAGAAGTTCATAAAAGTTTCAAATTAGTCACATTTGTTGAAAAAAAAAACATAAAAT GCAGGTGCCTGCAATGTTTACATTGTGTCAGATATTTCAGAGCCATGTATAATGTTGTCT TGAAATCCTAAACACTTAGTTTTTTAAAACATTTCTGGTTACTGAGGAGGCATAAAAAAT CCACAGGACTACATCTAAAATGAAGGCTGTCTTTCTCCTTTTATAAATACTTTTGATTCC TAACACAAAAATATGCACAGAAAGACATTTAGAATTTTCAATCCTTTTTAAAAATGAGGG ATACTGCTTTTGAAAATCTCTCTAACAGTTTTTGCTTGCCTTCTTCANCTCATTTTCTCC AAGAGCTTGCTAAGATGGCATCACCTGTTACTGAACTTTATTGCCTGGTCCAAACTGCGA GGTGGCATATAGCTACAGCTGATCTTAAATTACTCTGAAGGATCCTACCTCGCTGGACCC GACATGTTTCTGGCATACTAAACATGGCGGGTAGTTGTAAAA |
Restriction Sites | NotI-NotI |
ACCN | NM_182977 |
Insert Size | 4180 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_182977.1, NP_892022.1 |
RefSeq Size | 4232 bp |
RefSeq ORF | 3261 bp |
Locus ID | 23530 |
UniProt ID | Q13423 |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism |
Gene Summary | This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Restoration of Mitochondrial NAD(+) Levels Delays Stem Cell Senescence and Facilitates Reprogramming of Aged Somatic Cells
,Son, MJ;Kwon, Y;Son, T;Cho, YS;,
Stem Cells
,PubMed ID 27428041
[NNT]
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Other Versions
SKU | Description | Size | Price |
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RC224048 | NNT (Myc-DDK-tagged)-Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 7,560.00 |
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RC224048L3 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 10,260.00 |
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RC224048L4 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 10,260.00 |
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RG224048 | NNT (tGFP-tagged) - Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 9,160.00 |