Nck beta (NCK2) (NM_003581) Human Untagged Clone

CAT#: SC126794

NCK2 (untagged)-Human NCK adaptor protein 2 (NCK2), transcript variant 1



  "NM_003581" in other vectors (6)

CNY 7,220.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
NCK2 mouse monoclonal antibody,clone OTI4H7
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GRB4; NCKbeta
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_003581, the custom clone sequence may differ by one or more nucleotides


ATGACAGAAGAAGTTATTGTGATAGCCAAGTGGGACTACACCGCCCAGCAGGACCAGGAGCTGGACATCA
AGAAGAACGAGCGGCTGTGGTTGCTGGACGACTCCAAGACGTGGTGGCGGGTGAGGAACGCGGCCAACAG
GACGGGCTATGTACCGTCCAACTACGTGGAGCGGAAGAACAGCCTGAAGAAGGGCTCCCTCGTGAAGAAC
CTGAAGGACACACTAGGCCTCGGCAAGACGCGCAGGAAGACCAGCGCGCGGGATGCGTCCCCCACGCCCA
GCACGGACGCCGAGTACCCCGCCAATGGCAGCGGCGCCGACCGCATCTACGACCTCAACATCCCGGCCTT
CGTCAAGTTCGCCTATGTGGCCGAGCGGGAGGATGAGTTGTCCCTGGTGAAGGGGTCGCGCGTCACCGTC
ATGGAGAAGTGCAGCGACGGTTGGTGGCGGGGCAGCTACAACGGGCAGATCGGCTGGTTCCCCTCCAACT
ACGTCTTGGAGGAGGTGGACGAGGCGGCTGCGGAGTCCCCAAGCTTCCTGAGCCTGCGCAAGGGCGCCTC
GCTGAGCAATGGCCAGGGCTCCCGCGTGCTGCATGTGGTCCAGACGCTGTACCCCTTCAGCTCAGTCACC
GAGGAGGAGCTCAACTTCGAGAAGGGGGAGACCATGGAGGTGATTGAGAAGCCGGAGAACGACCCCGAGT
GGTGGAAATGCAAAAATGCCCGGGGCCAGGTGGGCCTCGTCCCCAAAAACTACGTGGTGGTCCTCAGTGA
CGGGCCTGCCCTGCACCCTGCGCACGCCCCACAGATAAGCTACACCGGGCCCTCGTCCAGCGGGCGCTTC
GCGGGCAGAGAGTGGTACTACGGGAACGTGACGCGGCACCAGGCCGAGTGCGCCCTCAACGAGCGGGGCG
TGGAGGGCGACTTCCTCATTAGGGACAGCGAGTCCTCGCCCAGCGACTTCTCCGTGTCCCTTAAAGCGTC
AGGGAAGAACAAACACTTCAAGGTGCAGCTCGTGGACAATGTCTACTGCATTGGGCAGCGGCGCTTCCAC
ACCATGGACGAGCTGGTGGAACACTACAAAAAGGCGCCCATCTTCACCAGCGAGCACGGGGAGAAGCTCT
ACCTCGTCAGGGCCCTGCAGTGA


>OriGene 5' read for NM_003581 unedited
TGTAACTTCAGAGGTGTAATTAGCTGAAAACATCATCGTTTTGAAGAGTTCTGCGTTTTG
CCAGTCACCTCTCAACTGTGTGCCAAAGAAGGACTCCATGAAAGATGACAGAAGAAGTTA
TTGTGATAGCCAAGTGGGACTACACCGCCCAGCAGGACCAGGAGCTGGACATCAAGAAGA
ACGAGCGGCTGTGGTTGCTGGACGACTCCAAGACGTGGTGGCGGGTGAGGAACGCGGCCA
ACAGGACGGGCTATGTACCGTCCAACTACGTGGAGCGGAAGAACAGCCTGAAGAAGGGCT
CCCTCGTGAAGAACCTGAAGGACACACTAGGCCTCGGCAAGACGCGCAGGAAGACCAGCG
CGCGGGATGCGTCCCCCACGCCCAGCACGGACGCCGAGTACCCCGCCAATGGCAGCGGCG
CCGACCGCATCTACGACCTCAACATCCCGGCCTTCGTCAAGTTCGCCTATGTGGCCGAGC
GGGAGGATGAGTTGTCCCTGGTGAAGGGGTCGCGCGTCACCGTCATGGAGAAGTGCAGCG
ACGGTTGGTGGCGGNGCAGCTACAACGGGCAGATCGGCTGGTTCCCCTCCAACTACGTCT
TGGAGGAAGTGGACGAAGCGGCTGCGGAGTCCCCAGCTTCCTGAGCCTGCGCAAGGCGCC
TCCCTGAGCATGGCCAGGGCTCCGCGTGCTGAATGTGTCCAAACGCTTACCCCTTCACTT
TATCCCGGAGGAGACCTAACTTTCAAAAGGGGAAACATGGAGTGATTGAAAAC
>OriGene 3' read for NM_003581 unedited
NAAATTTGACTTGGACCCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTCC
TTGGCATAATACTTTTTTTTATTACAATATCCAAAAAACTGGGTATGCAAGTTTAGGGGA
TCTCAAGACCCCTTCTTCAATCGTAGGAATGTGCCATCTCAAGACGTTATATTTAAACTA
TAAAGAAGTTCAAATGTAAAGGAAAAAGAAAATGCAATTTCTTCATAAACATTCTGTGTG
TCTCTTACTACCAATCACATACTCTTTTGTAAACCCTGAAAAATTTCCCTGTAAATAATA
TATATATATATAGTGTGTGTGTGTGTGTGTGTGTGTGCACGTGTGTGTGTGTATAAAGTG
TTGGTAGCTCCCTTCCCCAAAGATCAGCTGTTTCCCTTAATCATCTGTATTAGTGTCAAC
AAACGGCTACAGATACATATTTCTTTGAGAATTAAATACATAATTGTGAGATTCACACAA
GCCGAAGGGCAAGAGGAAAAAGCACTATGTGGATGGCACATTTGGGGTAAATTACCAAAA
CATCTGTCTTTCTGAGTCACCGGTTCTCTCTTTTGCAGACTGCCTTTCCAGGAAATTCTT
TCTTTATTGCAAGCACAGCCCCAAAGGCACTGGGCAGCCCCCTCACTGCCATCGCACCCG
GTGGCTCCAGCATTGCTATCACCAAACCTTTCGGCTCCCTGCCACACCCGGGTACCCCTG
AAGCGCCACAGGACATGNGCCGAGGCCTGCTAGGACTGGGGCCCCCGCCCCTGTTTTCCC
CACCCCTCTCGACGCCCCACAAACCGACGCCTGCCCTGGCGGCCCCCCAGGCCTCCCCCT
CGCCCTCCCGTCTCCCTCCTTCCTTCCCAGGGCGCCCTCCTCTCCTCTCACTATCCCCGC
CCCCCCACCCCCCTCCT
Restriction Sites NotI-NotI     
ACCN NM_003581
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003581.2, NP_003572.2
RefSeq Size 2698 bp
RefSeq ORF 1143 bp
Locus ID 8440
UniProt ID O43639
Domains SH2, SH3
Protein Families Druggable Genome
Protein Pathways Axon guidance, ErbB signaling pathway, Pathogenic Escherichia coli infection, T cell receptor signaling pathway
Gene Summary This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (A). Variants 1 and 2 both encode isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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