DUT (U90223) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	dUTPase
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC126787 sequence for U90223 edited (data generated by NextGen Sequencing) ATGACTCCCCTCTGCCCTCGCCCCGCGCTCTGCTACCATTTCCTTACGTCTCTGCTTCGC TCAGCGATGCAAAACGCGCGAGGNACGGCAGAGGGCCGAAGCCGCGGTACTCTCCGGGCC AGGCCCGCCCCTCGGCCGCNNGCGGCGCAGCACGGGATTCCCCGGCCGCTGTCCAGCGCT GGCCGCCTGAGCCAAGGCTGCCGCGGAGCCAGTACAGTCGGGGCCGCTGGCTGGAAGGGC GAGCTTCCTAAGGCGGGGGGAAGCCCGGCGCCGGGGCCGGAGACACCCGCCATTTCACCC AGTAAGCGGGCCCGGCCTGCGGAGGTGGGCGGCATGCAGCTCCGCTTTGCCCGGCTCTCC GAGCACGCCACGGCCCCCACCCGGGGCTCCGCGCGCGCCGCGGGCTACGACCTGTACAGT GCCTATGATTACACAATACCACCTATGGAGAAAGCTGTTGTGAAAACGGACATTCAGATA GCGCTCCCTTCTGGGTGTTATGGAAGAGTGGCTCCACGGTCAGGCTTGGCTGCAAAACAC TTTATTGATGTAGGAGCTGGTGTCATAGATGAAGATTATAGAGGAAATGTTGGTGTTGTA CTGTTTAATTTTGGCAAAGAAAAGTTTGAAGTCAAAAAAGGTGATCGAATTGCACAGCTC ATTTGCGAACGGATTTTTTATCCAGAAATAGAAGAAGTTCAAGCCTTGGATGACACCGAA AGGGGTTCAGGAGGTTTTGGTTCCACTGGAAAGAATTAA Clone variation with respect to U90223.1 84 c=>n;140 c=>n;141 g=>n 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for U90223 unedited TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAGCCTGGCGCACG TCCGGAGGTGCCGAGGACCCAACCAGCCCAAACTCTGGGGGAAATGACTCCCCTCTGCCC TCGCCCCGCGCTCTGCTACCATTTCCTTACGTCTCTGCTTCGCTCAGCGATGCAAAACGC GCGAGGCGCACGGCAGAGGGCCGAAGCCGCGGTACTCTCCGGGCCAGGCCCGCCCCTCGG CCGCGCCGCGCAGCACGGGATTCCCCGGCCGCTGTCCAGCGCTGGCCGCCTGAGCCAAGG CTGCCGCGGAGCCAGTACAGTCGGGGCCGCTGGCTGGAAGGGCGAGCTTCCTAAGGCGGG GGGAAGCCCGGCGCCGGGGCCGGAGACACCCGCCATTTCACCCAGTAAGCGGGCCCGGCC TGCGGAGGTGGGCGGCATGCAGCTCCGCTTTGCCCGGCTCTCCGAGCACGCCACGGCCCC CACCCGGGGCTCCGCGCGCGCCGCGGGCTACGACCTGTACAGTGCCTATGATTACACAAT ACCACCTATGGAGAAAGCTGTTGTGAAAACGGACATTCAGATAGCGCTCCCTTCTGGGGT GTNTATGGAAGAGTGGCTCCACGGTCAGGCTTGGCTGCAAAACACTNTATTGATGTANGA GCTGGTGTCATAGATGAAGATTATGAGGAAATGGNTGGGTGTTGTACTGGTTAATTTTGG CANAAANNAGTTTGAGTCANANAGGTGATCGAATTGACAGCTCATTTGCGAACGATTTTT TATCCAGAATANAAGAAGTTCAAGCTTGGATACACCNAAGGGTCCAGGGGAGGTTTTTGG TTCACTGGGAAGAATTNAAATTATGCCCAGACAGAAAACAGAGAGTCTACCCTTTTTCTA AAAAAAAAAAAAAC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for U90223 unedited GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAGAAAAAGTATGACTTCTT GTTTTCTGTTCTTGGCATAAATTTTAATTCTTTCCAGTGGAACCAAAACCTCCTGAACCC CTTTCGGTGTCATCCAAGGCTTGAACTTCTTCTATTTCTGGATAAAAAATCCGTTCGCAA ATGAGCTGTGCAATTCGATCACCTTTTTTGACTTCAAACTTTTCTTTGCCAAAATTAAAC AGTACAACACCAACATTTCCTCTATAATCTTCATCTATGACACCAGCTCCTACATCAATA AAGTGTTTTGCAGCCAAGCCTGACCGTGGAGCCACTCTTCCATAACACCCAGAAGGGAGC GCTATCTGAATGTCCGTTTTCACAACAGCTTTCTCCATAGGTGGTATTGTGTAATCATAG GCACTGTACAGGTCGTAGCCCGCGGCGCGCGCGGAGCCCCGGGTGGGGGCCGTGGCGTGC TCGGAGAGCCGGGCAAAGCGGAGCTGCATGCCGCCCACCTCCGCAGGCCGGGCCCGCTTA CTGGGTGAAATGGCGGGTGTCTCCCGCCCCGGCGCCGGGCTTCCCCCGCCTTAGGAAGCT CGCCCTTCCAGCCAGCGGCCCCGACTGTACTGGCTCCGCGGCAGCCTTTGCTTTGGGCGG CCAACCGCTGGACACGCGCCCGGGGAAATCCCCGTGCCTGCCCGGCGCCGCTCTAGGGGC GTGCCCGTGTCCCGAAAAGCACCCCGGTCTTGCGGCCTTTGTCCCCCCCCCTTCCCGGTT TTCCACCCTCTAATAACCTATACACCCCACCGATCGTCACTACCCCCCCCTCTGACTCCT TTCGTAGCACACCAGTTCTTCTCCACTTACACATTACTTTATATTCATCCCCCACCCACC CTTCTCCCGTTCCTCGTTATACAAATTACCAATTTCTCCCAAATCCGTCCACTTCT
Restriction Sites	NotI-NotI
ACCN	U90223
Insert Size	940 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	U90223.1, AAB94642.1
RefSeq Size	960 bp
RefSeq ORF	960 bp
Locus ID	1854
Domains	dUTPase
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Pyrimidine metabolism
Gene Summary	This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]

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