CUG BP1 (CELF1) (AK056024) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP; hNab50; NAB50; NAPOR
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK056024, the custom clone sequence may differ by one or more nucleotides TAAAGTGCAGCTCAAACGTTCGAAGAATGACAGCAAGCCCTACTGAGCGTGCTCCCCTCTGAGACTGGAG TGAGAGGGTCTTCTGGTAAGTGGGGGAGGAGCACCCTTAATGATTCGAAGCCCTGAGGCTGTGTGTTGAC AGCCCTGGACCCTGATCCCTGCCACTCTCGCAGGCACAGCTTGCCCTGAAGACTCGGCTACTGCCTTCTG TGGGAGTTTCGCTTCGTACAGAGGACAAGTTTGTGCTTTGGTTTCCAGTGTTTTACTTTGGAGTTTAGGT GCCATATCCTGAGGTTTTTTTTGTTTTTGTTTTTGTTTCTCCTTTATTTAAAGTTTGCTGTGTTTGTAAC CAGTGTGTGTTGAGAAGGACCAACACCAAACCACCCTGGGGAAGGGGGACAGGGAAACATTTAACACAGA CGATGATCAGGATTTGCCCCAAACCACCCCAAGAGAGAGGACTGAGTGGAACAAAAAAGTGACCCCCAGA ACCTCTCTTAGTGTGAGGGTGGGTAGAATGAGAACTGACACCTGGGAGCTGTGGGGAGCAGAGCGGCTTT GGGGAAGAGTGGAGTGACTAGACACCTAATGCCCTGGCAGCTGGAGACTCAAACTCCTGTACAGCTACCT TCTGGGAAATAGTTTTTGACACCTATTTTTCAGATTCTTGTCCGGAATTTCTGCTGCTCTTTTCAAAAAA GGGCATTAACAATTCTCTGGAAATAAAGCACCTGTTAGCCTGACATATGCAAAAAGCAGGGCGGCATCAC CCATTACGAGCTCCCCCAGCCAGCAGTCAGTATTGGATTGGCCTTGCCTGGCTGGTGGCAGTTTGGGAGA AACAGCCAAAGAGGTTAGTTTATTTCAACACCAAATTAGACCATGGCCCATTTCCAACGGGTCTCTTTAA AGGCCTCTATGGAATACATTGCCTGGTTTCCTTCTTTGCAGTTCACCACAGCAAGGAATGTCACCTCCAT CCCAAGCCACCATTTTCTCATGAAGGCAAATCCAAGAAGGGCTTGCAGTTCTTGCTGAAGGGGGTACCAT TTGTGGGCAGAGTGATCAATACCATCTAGTTGGGGGAGGAGGAGCTTATTTCTTGGTGTACTTGAATCAG AAGGTCCCTGCAAGCCAGTATGCTTCATTTGCCAGTGGCCAGAAATTCTCCCTTGCCTCCTTGATTGAGG TGTCCCAGATGTAGTATTCCCACAGGGGTCTGGCAGGCCCCTCCTGTAACCACTCCAGTCACATTTTCTG CTCTTGAGGCAGAGGTGACATCAGGACGTTTACAGCCTCCACATGAATTGAGTGTTCATTTACCTCAGTA TTACCGTGTTCATTTTTGTCCTCGTGCTACAGTTAGCTCCCTGCCGCCTCTTGCAGGTCTTACTTCAGCT CTTGCCTGTGACCCACACAGCTTCTGGGCTCTGCCTCTGCTCTAAGAAGTGCTGTGGGGGTAGAGAACCA GGAAGGACATGCTGTTGGGAATGTACACCTGGGCAGAGGTGGCCCTGTTAAGATGTGACTGTAGCCACAG CCAGGGATGGGATCCCTGGGTCTCTGGAATCTGAAACCTCAACAAATACCAGTCTTGACCCCTAGCAGCA GAGATAAGAATAAAGGGGTTGGTTTGCTATTAAGTCAGATGGGGGCTCTCTCCTTGTCATGCTGTCCCTG TGGGTAACAGACAGGGATTGACCATCTTACTGTTGTACAGGTAGCTGGTGGTGTTGGATTATCCCATTGA AGCTGGGACAGCTGTTCCTTTCTCATAGTGATAACTTGGGTCTGTTGTCCTCTAAGGAACGTGAAACATA CACTTCTTAAGCTGAACCACGTAAACTTGAAATTCTGCGCACTGGGAGAAATGTGTCCTGTGTTTCAAAG GATAAAACTGTTCTAAAGGCTTCCAGGGTCATGATGGGATTTTTTAAATAAATGCAAAAAAT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK056024 unedited TACGACTACTATAGGGCGGCCGCGAATTCGCACGAGGCACCTGTTAGCCTGACATATGCA AAAAGCAGGCGGCATCACCCATTACGAGCTCCCCCAGCCAGCAGTCAGTATTGGATTGGC CTTGCCTGGCTGGTGGCAGTTTGGGAGAAACAGCCAAAGAGGTTAGTTTATTTCAACACC AAATTAGACCATGGCCCATTTCCAACGGGTCTCTTTAAAGGCCTCTATGGAATACATTGC CTGGTTTCCTTCTTTGCAGTTCACCACAGCAAGGAATGTCACCTCCATCCCAAGCCACCA TTTTCTCATGAAGGCAAATCCAAGAAGGGCTTGCAGTTCTTGCTGAAGGGGGTACCATTT GTGGGCAGAGTGATCAATACCTCTTAGTTGGGGGAGGAGGAGCTTATTTCTTGGTGTACT TGAATCAGAAGGTCCCTGCAAGCCAGTATGCTTCATTTGCCAGTGGCCAGAAATTCTCCC TTGCCTCCTTGATTGAGGTGTCCCAGATGTAGTATTCCCACAGGGGTCTGGCAGGCCCCT CCTGTAACCACTCCAGTCACATTTTCTGCTCTTGAGGCAGAGGTGACATCAGGACGTTTA CAGCCTCCACATGAATTGAGTGTTCATTTACCTCAGTATTACCGTGTTCATTTTTGTCCT CGTGCTACAGTTAGCTCCCTGCCGCCTCTTGCANGTCTTACTTCAGCTCTTGCCTGTGAC CCACACAGCTTCTGGGCTCTGCCTCTGCTCTAAGAAGTGCTGTGGGGGTAGAAGACCAGG AAGGACATGCTGTTGGGAATGTACACCTNNGGCAGAGTGGCCCTGTTAAGATGTGACTGT AGCCCAGCCAGGGATGGGATCCCTGGGTCTCTGAATCTGAAACCTCACAATACAGTCTTG ACCCTACAGCAAAAGAATAAAGGGGGTGTTGCTATAGTAGATGGGCTTCTCTGTATCTGC CTGTGGACANACAGAN
Restriction Sites	NotI-NotI
ACCN	AK056024
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK056024.1
RefSeq Size	1952 bp
RefSeq ORF	1952 bp
Locus ID	10658
Protein Families	Druggable Genome
Gene Summary	Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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