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Glycerol kinase (GK) (NM_000167) Human Untagged Clone
CAT＃: SC126764
GK (untagged)-Human glycerol kinase (GK), transcript variant 2
CNY 3,912.00
CNY 8,460.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GK1; GKD |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126764 sequence for NM_000167 edited (data generated by NextGen Sequencing)
ATGGCAGCCTCAAAGAAGGCAGTTTTGGGGCCATTGGTGGGGGCGGTGGACCAGGGCACC AGTTCGACGCGCTTTTTGGTTTTCAATTCAAAAACAGCTGAACTACTTAGTCATCATCAA GTAGAAATAAAACAAGAGTTCCCAAGAGAAGGATGGGTGGAACAGGACCCTAAGGAAATT CTACATTCTGTCTATGAGTGTATAGAGAAAACATGTGAGAAACTTGGACAGCTCAATATT GATATTTCCAACATAAAAGCTATTGGTGTCAGCAACCAGAGGGAAACCACTGTAGTCTGG GACAAGATAACTGGAGAGCCTCTCTACAATGCTGTGGTGTGGCTTGATCTAAGAACCCAG TCTACCGTTGAGAGTCTTAGTAAAAGAATTCCAGGAAATAATAACTTTGTCAAGTCCAAG ACAGGCCTTCCACTTAGCACTTACTTCAGTGCAGTGAAACTTCGTTGGCTCCTTGACAAT GTGAGAAAAGTTCAAAAGGCCGTTGAAGAAAAACGAGCTCTTTTTGGGACTATTGATTCA TGGCTTATTTGGAGTTTGACAGGAGGAGTCAATGGAGGTGTCCACTGTACAGATGTAACA AATGCAAGTAGGACTATGCTTTTCAACATTCATTCTTTGGAATGGGATAAACAACTCTGC GAATTTTTTGGAATTCCAATGGAAATTCTTCCAAATGTCCGGAGTTCTTCTGAGATCTAT GGCCTAATGAAAGCTGGGGCCTTGGAAGGTGTGCCAATATCTGGGTGTTTAGGGGACCAG TCTGCTGCATTGGTGGGACAAATGTGCTTCCAGATTGGACAAGCCAAAAATACGTATGGA ACAGGATGTTTCTTACTATGTAATACAGGCCATAAGTGTGTATTTTCTGATCATGGCCTT CTCACCACAGTGGCTTACAAACTTGGCAGAGACAAACCAGTATATTATGCTTTGGAAGGT TCTGTAGCTATAGCTGGTGCTGTTATTCGCTGGCTAAGAGACAATCTTGGAATTATAAAG ACCTCAGAAGAAATTGAAAAACTTGCTAAAGAAGTAGGTACTTCTTATGGCTGCTACTTC GTCCCAGCATTTTCGGGGTTATATGCACCTTATTGGGAGCCCAGCGCAAGAGGGATAATC TGTGGACTCACTCAGTTCACCAATAAATGCCATATTGCTTTTGCTGCATTAGAAGCTGTT TGTTTCCAAACTCGAGAGATTTTGGATGCCATGAATCGAGACTGTGGAATTCCACTCAGT CATTTGCAGGTAGATGGAGGAATGACCAGCAACAAAATTCTTATGCAGCTACAAGCAGAC ATTCTGTATATACCAGTAGTGAAGCCCTCAATGCCCGAAACCACTGCACTGGGTGCGGCT ATGGCGGCAGGGGCTGCAGAAGGAGTCGGCGTATGGAGTCTCGAACCCGAGGATTTGTCT GCCGTCACGATGGAGCGGTTTGAACCTCAGATTAATGCGGAGGAAAGTGAAATTCGTTAT TCTACATGGAAGAAAGCTGTGATGAAGTCAATGGGTTGGGTTACAACTCAATCTCCAGAA AGTGGTATTCCATAA Clone variation with respect to NM_000167.5 >OriGene 5' read for NM_000167 unedited
ATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCGGCCTCGATCTCTGGACTCG TCACCTGCCCCTCCCCCTCCCGCCGCCGTCACCCAGGAAACCGGCCGCAATCGCCGGCCG ACCTGAAGCTGGTTTCATGGCAGCCTCAAAGAAGGCAGTTTTGGGGCCATTGGTGGGGGC GGTGGACCAGGGCACCAGTTCGACGCGCTTTTTGGTTTTCAATTCAAAAACAGCTGAACT ACTTAGTCATCATCAAGTAGAAATAAAACAAGAGTTCCCAAGAGAAGGATGGGTGGAACA GGACCCTAAGGAAATTCTACATTCTGTCTATGAGTGTATAGAGAAAACATGTGAGAAACT TGGACAGCTCAATATTGATATTTCCAACATAAAAGCTATTGGTGTCAGCAACCAGAGGGA AACCACTGTAGTCTGGGACAAGATAACTGGAGAGCCTCTCTACAATGCTGTGGTGTGGCT TGATCTAAGAACCCAGTCTACCGTTGAGAGTCTTAGTAAAAGAATTCCAGGAAATAATAA CTTTGTCAAGTCCAAGACAGGCCTTCCACTTAGCACTTACTTCAGTGCAGTGAAACTTCG TTGGCTCCTTGACAATGTGAGAAAAGTTCAAAGGCCGTTGAAGAAAACGAGCTCTTTNTG GGACTATTGATTCATGGCTATTTTGNAGTTTGACAGGAGAGTAATGGGAGTGTCCACTGT ACAGATGTAACAATGCAGTAGGACTATGCTTTTCACATTCATTTTTGGAATGGGATAAAC ACTCTGCGAATTTTTTGGATTCATGGNAATCTTCCATGTTCCGGAGTCTTTTGAAACTAT GCTATGAAATCTTCATACTAAACTTGGCCTTGAAGGGGCATTTTTGGGTTTTAGGGGACA CTTCTTTTGTGGACAAATGTTTCCTGGGCAGCAAAATGGAAAGAGGTTTCTTTTACGCAC AACGGGTTTTTTATT >OriGene 3' read for NM_000167 unedited
TATTTTGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAGAACAAGG GGTCAATTTATTTTAAGTAAATTTGTCATTAAATATCAATGTGTACAATTTCAAACATTT ATAAATCTTCCAATTTATAGAAAAATTAATGTAATCTTAAAATTATTTGAACAGTGGCTT TCACAATTATTTACAATGAACTCAACCTACTTTTGTAGAAACATTTATGTTTAAAAGTAC CCCTGCCACAAAGTGATCATTTCACAGTACCTTTCTATAATATTTATGTCACTTAAGTGG TGGCTGCATCAATAAAACCTAGCAGATTCAATAAATTAGAAACAGCACAATCAGAACAGT CAATAAAGCTACCATTGATTCCAAACAAAACAGAACCTTTTATAGTAAGATATAAATGTT TTCCCATTCAGCACTCATTGAAAAAAATAATTAGGAGGCAATCTTTTCTTCACTGTTTAC TACAAAACAAGGTAATTTTAAGATATTTTTAAAAATTGATGTTAAGAAGCAGGATTAGGT AACTTCTTATAATATGTGACATTAAATATTTTGAGAAATTCTTGGTTTCATTGGAAGGTA TTTGTAATGATAAACCTTCTTTCAGTTATAATGAGATACTAAGCAGAGGTAATTCTGTCA TCCAGATAGCAATTTCTATTAACATTGATACATTTGACTGAAGAGATTCTGGCTTATTCT ATATTTTTCTACTAGGATTTACCTTTTTGAAACCTCTACCCCTCATAGGATCAAATATGC TAAATTAATCAAAAAAAAAACCATTGAATATTGTTGGGTTGAAACATATTACTGAATATC CACAGTCTCTCTTTTTAGCTTAACATAATAAAAAGGAGGAAAGAATATTTGTATCCACCA AAAATTTCTTTTGACAAACAAAATTAAGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000167 |
| Insert Size | 3520 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000167.3, NP_000158.1 |
| RefSeq Size | 3573 bp |
| RefSeq ORF | 1575 bp |
| Locus ID | 2710 |
| UniProt ID | P32189 |
| Domains | FGGY |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Metabolic pathways, PPAR signaling pathway |
| Gene Summary | The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. This results in a shorter isoform (b) missing a 6 aa protein segment compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213688 | GK (Myc-DDK-tagged)-Human glycerol kinase (GK), transcript variant 2 |
CNY 3,904.00 |
|
| RC213688L3 | Lenti ORF clone of Human glycerol kinase (GK), transcript variant 2, Myc-DDK-tagged |
CNY 6,370.00 |
|
| RC213688L4 | Lenti ORF clone of Human glycerol kinase (GK), transcript variant 2, mGFP tagged |
CNY 6,370.00 |
|
| RG213688 | GK (tGFP-tagged) - Human glycerol kinase (GK), transcript variant 2 |
CNY 5,504.00 |
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